• Imaging Science in Dentistry
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• 제51권4호
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• pp.429-438
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• 2021

Purpose: This study investigated the accuracy of laser-scanned models and 3-dimensional(3D) rendered cone-beam computed tomography (CBCT) compared to the gold standard (plaster casts) for linear measurements on dental arches. Materials and Methods: CBCT scans and plaster models from 30 patients were retrieved. Plaster models were scanned by an Emerald laser scanner (Planmeca, Helsinki, Finland). Sixteen different measurements, encompassing the mesiodistal width of teeth and both arches' length and width, were calculated using various landmarks. Linear measurements were made on laser-scanned models using Autodesk Meshmixer software v. 3.0 (Autodesk, Mill Valley, CA, USA), on 3D-rendered CBCT models using OnDemand 3D v. 1.0 (Cybermed, Seoul, Korea) and on plaster casts by a digital caliper. Descriptive statistics, the paired t-test, and intra- and inter-class correlation coefficients were used to analyze the data. Results: There were statistically significant differences between some measurements on plaster casts and laser-scanned or 3D-rendered CBCT models (P<0.05). Molar mesiodistal width and mandibular anterior arch width deviated significantly different from the gold standard in both methods. The largest mean differences of laser-scanned and 3D-rendered CBCT models compared to the gold standard were 0.12±0.23 mm and 0.42±0.53 mm, respectively. Most of the mean differences were not clinically significant. The intra- and inter-class correlation results were acceptable for all measurements(>0.830) and between observers(>0.801). Conclusion: The 3D-rendered CBCT images and laser-scanned models were useful and accurate alternatives to conventional plaster models. They could be used for clinical purposes in orthodontics and prostheses.

• Physical Therapy Rehabilitation Science
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• 제6권3호
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• pp.152-157
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• 2017

Objective: The aim of this study was to determine the intra-rater and inter-rater reliability of the dual rehabilitative ultrasound imaging (D-RUSI) when simultaneously measuring muscle thickness changes at rest and during co-contraction of the biceps brachii (BB) and triceps brachii (TB). Design: Cross-sectional study. Methods: This study included 36 healthy participants (23 men, 13 women). The participants sat on a chair in a comfortable position with a cushion placed under their elbow to maintain a 90-degree elbow flexion angle. The muscle thickness of the biceps brachii and triceps brachii was measured twice using the D-RUSI by two examiners during resting and co-contraction states. One week later, the same procedure was performed once again. Results: The intra-class correlation coefficients (ICCs) for intra-rater reliability ranged from 0.887 to 0.989 and the confidence interval was within an acceptable range of 0.778 to 0.994. The standard error of measurement (SEM) values ranged from 0.303 to 0.866 and the minimal detectable change (MDC) values ranged from 0.84 to 2.40. The ICCs for inter-rater reliability ranged from 0.758 to 0.925. The SEM values ranged from 0.702 to 1.486 and the MDC values ranged from 1.95 to 4.12. Conclusions: The use of the D-RUSI of the BB muscle had a very good intra-rater reliability and very good inter-rater reliability at the resting state, and a, good inter-rater reliability at the co-activation state. ICC values showed very good intra-reliability and inter-reliability for the TB muscle. the D-RUSI is a useful tool for simultaneously measuring the thickness of two muscles when the BB is an agonist and the TB is an antagonist during co-activation of the upper arm.

• The Journal of Korean Physical Therapy
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• 제23권4호
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• pp.59-66
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• 2011

Purpose: The objective of this study was to evaluate the inter- and intra-examiner reliability of ultrasonography (US) for measuring the thicknesses and cross-sectional area of the longus colli muscle (LCM) in healthy subjects. Methods: Twenty-five healthy adults participated in this study as subjects and two examiners attended to the study. LCM size was measured at the level of the thyroid cartilage. All subjects were randomly allocated to one of the three different methods. Method 1 was that the examiner A conducts the first measurement and one hour later conducts the second measurement and twenty four hours later conducts the third measurement, respectively. Method 2 was that the examiner B conducts the measurements in the same way as the examiner A. Method 3 was that the examiners A and B randomly conduct the measurements twice at an interval of one hour. Results: The intra-class correlation coefficient (ICC) for the intra-examiner reliability ranged from 0.78 to 0.98 at rest and 0.57 to 0.98 during contraction for the examiner A. The $ICC_{3,3}$ for the intra-examiner reliability ranged from 0.60 to 0.96 at rest and 0.57 to 0.92 during contraction for the examiner B. The $ICC_{2,3}$ for the inter-examiner reliability ranged from 0.80 to 0.91 at rest and 0.84 to 0.93 during contraction. Conclusion: The findings of this study indicate that US is a reliable method for measuring the LCM size.

• Journal of Preventive Medicine and Public Health
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• 제41권3호
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• pp.195-199
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• 2008

Objectives : Malondialdehyde (MDA), a lipid peroxidation by-product, has been widely used as an indicator of oxidative stress. Urinary 2-naphthol, a urinary PAH metabolite, is used as a marker of ambient particulate exposure and is associated with lung cancer and chronic obstructive pulmonary disease. However, the stability and intra-individual variation associated with urinary MDA and 2-naphthol have not been thoroughly addressed. The objective of this study was to assess the stability and intra-individual variation associated with urinary MDA and 2-naphthol. Methods : Urine samples were collected from 10 healthy volunteers (mean age 34, range $27{\sim}42$ years old). Each sample was divided into three aliquots and stored under three different conditions. The levels of urinary MDA and 2-naphthol were analyzed 1) just after sampling, 2) after storage at room temperature ($21^{\circ}C$) for 16 hours, and 3) after storage in a $-20^{\circ}C$ freezer for 16 hours. In addition, an epidemiological study was conducted in 44 Chinese subjects over a period of 3 weeks. The urinary MDA and 2-naphthol were measured by HPLC three times. Results : There was no difference in the levels of urinary MDA and 2-naphthol between the triplicate measurements (n=10, p=0.84 and p=0.83, respectively). The intra-class correlation coefficients (ICC) for urinary MDA and 2-naphthol were 0.74 and 0.42, respectively. However, the levels of PM2.5 in the air were well correlated with the levels of both MDA and 2-naphthol in the epidemiological study. Conclusions : These results suggest that urinary MDA and 2-naphthol remain stable under variable storage conditions, even at room temperature for 16 hours, and indicate that these markers can be used in epidemiological studies involving various sample storage conditions. The intra-CC of urinary 2-naphthol and MDA were acceptable for application to epidemiological studies.

• 한국산학기술학회논문지
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• 제18권5호
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• pp.467-474
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• 2017

본 연구의 목적은 만성 뇌졸중 환자 8자 모양 경로 보행 검사(Figure-of-Eight Walk Test, F8WT)의 측정자간 절대적 신뢰도와 타당도를 알아보고자 하였다. 만성 뇌졸중 환자 36명을 대상으로 하였으며 F8WT 소요 시간의 측정자간 신뢰도는 급간내상관계수(Intra Class Coefficient, $ICC_{2,1}$)를 구하였고, 절대적 신뢰도는 측정의 표준 오차값(Standard Error Measurment, SEM)과 최저 실제 차이(Small Real Difference, SRD)를 구하였다. F8WT 소요시간의 타당도는 뇌졸중 자세 평가 척도(Postural Assessment Scale for Stroke, PASS)와 체간 장애 척도(Trunk Impairment Scale, TIS)간의 스피어만 상관 계수(Spearman Correlation Coefficient)를 구하였다. 연구 결과 F8WT 소요 시간의 측정자간 신뢰도 ICC=0.95(0.91~0.97)로 매우 높았고, SEM과 SRD는 각각 1.02점, 2.82점으로 수용할 만 하였다. F8WT 소요 시간은 PASS(r=-0.82), TIS(r=-0.85)와 유의한 상관관계가 있는 것으로 나타났으며, 뇌졸중 환자의 특이성 장애인 자세 및 체간 조절과 유의한 관련성이 있는 것으로 확인되었다. 따라서 F8WT는 만성 뇌졸중 환자의 보행 능력을 평가하는데 유용한 평가 도구로 사용될 수 있을 것이다.

• Physical Therapy Rehabilitation Science
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• 제5권3호
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• pp.155-161
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• 2016

Objective: This study aimed to investigate the reliability and validity of a personal computer-based muscle viewer (PC-BMW) compared with that of a portable ultrasound (P-US) for measuring upper trapezius (UT) and transversus abdominis (TrA) muscle thickness at rest and during contraction. Design: Observational inter-rater reliability study. Methods: Fifty-five healthy participants (25 men, 30 women) participated in this study. PC-BMW and P-US were randomly measured at the UT and TrA muscles. Two examiners randomly obtained the images of all participants in 3 test sessions lasting 2 days. Intra-class correlation coefficients (ICCs), standard error of measurement, contraction ratio, and correlation were used to estimate reliability and validity. Pearson's correlation coefficients were used to analyze the relationship between muscle thickness measures taken from PC-BMW and P-US. Results: The intra-rater reliability ICCs of UT and TrA muscle thickness for the PC-BMW were >0.995, indicating excellent reliability. Inter-rater reliability ICCs for the PC-BMW ranged from 0.963 to 0.987. The P-US also exhibited high reliability. A high correlation was found between the measurements of the two muscles in PC-BMW and P-US (p<0.01). Conclusions: PC-BMW provides clear and excellent images, is pocket-sized and less expensive than a conventional ultrasound imaging system. PC-BMW can be utilized variously and has the advantage of rehabilitative ultrasound imaging. More research is needed to evaluate the utility of PC-BMW for rehabilitation.

• IMMUNE NETWORK
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• 제23권2호
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• pp.17.1-17.16
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• 2023

Latent membrane protein 2A (LMP2A), a latent Ag commonly expressed in Epstein-Barr virus (EBV)-infected host cells, is a target for adoptive T cell therapy in EBV-associated malignancies. To define whether individual human leukocyte antigen (HLA) allotypes are used preferentially in EBV-specific T lymphocyte responses, LMP2A-specific CD8⁺ and CD4⁺ T cell responses in 50 healthy donors were analyzed by ELISPOT assay using artificial Ag-presenting cells expressing a single allotype. CD8⁺ T cell responses were significantly higher than CD4⁺ T cell responses. CD8⁺ T cell responses were ranked from highest to lowest in the order HLA-A, HLA-B, and HLA-C loci, and CD4⁺ T cell responses were ranked in the order HLA-DR, HLA-DP, and HLA-DQ loci. Among the 32 HLA class I and 56 HLA class II allotypes, 6 HLA-A, 7 HLA-B, 5 HLA-C, 10 HLA-DR, 2 HLA-DQ, and 2 HLA-DP allotypes showed T cell responses higher than 50 spot-forming cells (SFCs)/5×105 CD8⁺ or CD4⁺ T cells. Twenty-nine donors (58%) showed a high T cell response to at least one allotype of HLA class I or class II, and 4 donors (8%) had a high response to both HLA class I and class II allotypes. Interestingly, we observed an inverse correlation between the proportion of LMP2A-specific T cell responses and the frequency of HLA class I and II allotypes. These data demonstrate the allele dominance of LMP2A-specific T cell responses among HLA allotypes and their intra-individual dominance in response to only a few allotypes in an individual, which may provide useful information for genetic, pathogenic, and immunotherapeutic approaches to EBV-associated diseases.

• The Journal of Korean Physical Therapy
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• 제22권4호
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• pp.57-64
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• 2010

Purpose: This study aimed to determine whether there are differences in subtalar joint range of motion (ROM) when using different measurement methods, and to determine inter- and intra-rater reliability of goniometry as used in clinical setting. Methods: Subjects were thirty-one healthy males and females (sixty-two ankles) living in Korea. Three raters with different clinical experiences measured inversion and eversion range of motion of the subtalar joint two times. Measurements were done with subjects prone (open kinetic chain) and standing (closed kinetic chain). Rater and measurement methods were based on analyzing differences in range of motion. Intra-class correlation coefficients (ICCs) were calculated to determine intra-rater and inter-rater reliability. Results: Mean subtalar jont range of motion for inversion ranged from $9.31^{\circ}$ to $11.94^{\circ}$ for eversion, it ranged from $6.73^{\circ}$ to $9.20^{\circ}$. The differences in ROM between raters and between measurement methods were significant (p<0.01). The ICCs for interrater reliability ranged from $0.02^{\circ}$ to $0.20^{\circ}$ for inversion and from $0.23^{\circ}$ to $0.39^{\circ}$ for eversion. Intrarater reliability ranged from $0.32^{\circ}$ to $0.78^{\circ}$ for inversion and from $0.45^{\circ}$ to $0.73^{\circ}$ for eversion. Conclusion: Subtalar joint inversion and eversion ROM show differences for measurement methods low reliability between different raters, and low to high intra-rater reliability within sessions.

• 한국전문물리치료학회지
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• 제21권3호
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• pp.20-27
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• 2014

The purpose of this study was to compare the intra-rater and inter-rater reliability of three methods for measuring iliotibial tract length in 40 knees of 20 subjects. The measurements were performed by two examiners and one assistant. The length of the iliotibial tract was measured using three methods: the first used a modified Ober test with an inclinometer, the second used a modified Ober test with a marker, and the third used active adduction test with a marker in the supine position. Each examiner used the three methods in two sessions. For the first method, the inclinometer value was recorded manually. For the second and third methods, Image J analysis software was used to analyze the photographs taken. The intra-rater and inter-rater reliability of the measured value was assessed by means of intra-class correlation coefficients [ICC (3,1)] and the standard error of measurement. The intra-rater reliabilities of the three methods were excellent (ICC=.97, .95, and .94, respectively), and the inter-rater reliabilities of the first and second methods were fair to good (ICC=.64 and .65, respectively). The inter-rater reliability of the third method was excellent (ICC=.88). Our results suggest that the active adduction test is an objective and qualitative measurement method for measuring iliotibial tract length. We recommend that the active adduction test be used for measuring iliotibial tract length in the clinical setting.

• 한국전문물리치료학회지
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• 제21권3호
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• pp.73-79
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• 2014

The purposes of the current study were to (1) estimate the inter-rater agreement for visual assessment of scapular downward rotation (SDR), (2) develop the scapular downward rotation index (SDRI) as a method to measure SDR objectively and quantitatively, and (3) analyze the intra- and inter-rater reliability of the SDRI. Twenty subjects with scapular downward rotation syndrome (SDRS) were recruited for this study. The visual assessment and the measurement for the SDRI were conducted by two examiners in two sessions each. The SDRI [$(a-b){\div}a{\times}100$] is calculated with the measurement of two linear distances: One is a perpendicular distance from the root of the scapular spine to the thoracic mid-line (a), and the other is a perpendicular distance from the inferior angle of the scapula to the thoracic mid-line (b). Cohen's kappa coefficient was calculated to estimate the inter-rater agreement for visual assessment. Intra-class correlation coefficients (ICCs) with a 95% confidence interval (CI), the standard error of measurement, and minimal detectable differences were calculated to assess intra- and inter-rater reliability of SDR measurement using the SDRI. The results indicated that the kappa coefficient of inter-rater agreement for visual assessment was fair (${\kappa}=.21$). The intra-rater reliability of SDR measurement using the SDRI was excellent for examiner 1 (ICC=.92, 95% CI=.78~.97) and good for examiner 2 (ICC=.82, 95% CI=.55~.93). The inter-rater reliability was moderate (ICC=.73, 95% CI=.32~.89). These findings showed that SDR measurement using the SDRI for subjects with SDRS may be considered reliable and better than the visual assessment.