• Title/Summary/Keyword: interaction sites

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Fabrication of DNA Chip Using a Hydrophobic Template (소수성 Template를 이용한 DNA칩의 제작)

  • Choi, Yong-Sung;Moon, Jong-Dae;Lee, Kyung-Sup
    • Proceedings of the KIEE Conference
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    • 2006.07c
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    • pp.1315-1316
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    • 2006
  • Microarray-based DNA chips provide an architecture for multi-analyte sensing. In this paper, we report a new approach for DNA chip microarray fabrication. Multifunctional DNA chip microarray was made by immobilizing many kinds of biomaterials on transducers (particles). DNA chip microarray was prepared by randomly distributing a mixture of the particles on a chip pattern containing thousands of m-scale sites. The particles occupied a different sites from site to site. The particles were arranged on the chip pattern by the random fluidic self-assembly (RFSA) method, using a hydrophobic interaction for assembly.

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Development of DNA Chip Microarray Using Hydrophobic Template (소수성 Template를 이용한 DNA Chip Microarray의 개발)

  • Choi, Yong-Sung;Park, Dae-Hee
    • Proceedings of the KIEE Conference
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    • 2004.11a
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    • pp.271-274
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    • 2004
  • Microarray-based DNA chips provide an architecture for multi-analyte sensing. In this paper, we report a new approach for DNA chip microarray fabrication. Multifunctional DNA chip microarray was made by immobilizing many kinds of biomaterials on transducers (particles). DNA chip microarray was prepared by randomly distributing a mixture of the particles on a chip pattern containing thousands of m-scale sites. The particles occupied a different sites from site to site. The particles were arranged on the chip pattern by the random fluidic self-assembly (RFSA) method, using a hydrophobic interaction for assembly.

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Fabrication of Hydrophobic/Hydrophilic Pattern as a Template for DNA Chip Microaray (DNA Chip Microarrays를 위한 template로서 소수성 패턴의 제작)

  • Choi, Yong-Sung;Park, Dae-Hee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.11a
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    • pp.472-475
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    • 2004
  • Microarray-based DNA chips provide an architecture for multi-analyte sensing. In this paper, we report a new approach for DNA chip microarray fabrication. Multifunctional DNA chip microarray was made by immobilizing many kinds of biomaterials on transducers (particles). DNA chip microarray was prepared by randomly distributing a mixture of the particles on a chip pattern containing thousands of m-scale sites. The particles occupied a different sites from site to site. The particles were arranged on the chip pattern by the random fluidic self-assembly (RFSA) method, using a hydrophobic interaction for assembly.

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Molecular Dynamics Simulation for Bilayers of Alkyl Thiol Molecules at Solid-Solid Interfaces

  • 이송희;김한수;박형숙
    • Bulletin of the Korean Chemical Society
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    • v.19 no.10
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    • pp.1047-1054
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    • 1998
  • We present the results of molecular dynamics simulations for three different systems of bilayers of long-chain alkyl thiol [S(CH2)15CH3] molecules on an solid-solid interface using the extended collapsed atom model for the chain-molecule. It is found that there exist two possible transitions: a continuous transition characterized by a change in molecular interaction between sites of different chain molecules with increasing area per molecule and a sudden transition from an ordered lattice-like state to a liquid-like state due to the lack of interactions between sites of chain molecules on different surfaces with increasing distance between two solid surfaces. The third system displays a smooth change in probability distribution characterized by the increment of gauche structure in the near-tail part of the chain with increasing area per molecule. The analyses of energetic results and chain conformation results demonstrate the characteristic change of chain structure of each system.

New record of Folsomia quadrioculata(Tullberg, 1871) and redescription of Folsomia octoculata(Handschin, 1925) from the forest of South Korea

  • Lee, Yun-Sik;Cho, Kijong;Park, Kyung-Hwa
    • Korean Journal of Environmental Biology
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    • v.37 no.1
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    • pp.1-7
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    • 2019
  • During a recent study to understand an interaction of Collembola community and their environmental factors, two species in the genus Folsomia, F. quadrioculata Tullberg 1871 and F. octoculata Handschin 1925, were found in the forest of South Korea. From 2013 to 2015, these two species were sampled from six mountains and F. quadrioculata or F. octoculata was the dominant species in 5 sampling sites among 6 sampling sites. F. quadriocualta and F. octoculata were found in litter of deciduous and pine forest, respectively. Therefore, the major tree species seems to be one of the important factors determining their habitat. F. quadiroculata is newly recorded from the Korean Peninsula and F. octoculata is redescribed based on new materials from South Korea.

Movie Experience Sharing on Social Networking Sites of Cinema: Interplay between Telepresence and Customer Delight

  • Zong-Yi Zhu;Hyeon-Cheol Kim
    • International journal of advanced smart convergence
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    • v.12 no.4
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    • pp.224-236
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    • 2023
  • This study aims to investigate the effects of telepresence on young moviegoers' flow experiences and social interactions, and the impact on consumer delight, trust, and experience sharing behavior on cinema mobile social network site pages. Given the scarcity of telepresence research, indirect telepresence on experience sharing via two experiences and social interactions is also included. The study used pages from Korean cinema mobile social network sites, and 175 Chinese moviegoers residing in Korea participated. We found that telepresence positively impacts the activity in both human-human and human-computer interactions. We further contend that telepresence positively affects perceived enjoyment and attentional focus. However, perceived enjoyment does not significantly affect consumer delight. We found that consumer delight positively influences consumer trust and movie experience sharing. Moreover, we illustrated that telepresence significantly and indirectly influences consumer movie experience-sharing behavior through attention focus and consumer delight. Our results provide crucial insights for future study and practical managerial.

Kinetic Mechanism of Nucleotide Binding to Escherichia coli Transcription Termination Factor Rho: Stopped-flow Kinetic Studies Using ATP and Fluorescent ATP Analogues

  • Kim, Dong-Eun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.1
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    • pp.23-34
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    • 2004
  • Escherichia coli transcription termination factor Rho catalyzes the unwinding of RNA/DNA duplex in reactions that are coupled to ATP binding and hydrolysis. Fluorescence stopped-flow methods using ATP and the fluorescent 2'(3')-O-( N-methylanthraniloyl) derivatives (mant-derivatives) of ATP and ADP were used to probe the kinetics of nucleotide binding to and dissociation from the Rho-RNA complex. Presteady state nucleotide binding kinetics provides evidence for the presence of negative cooperativity in nucleotide binding among the multiple nucleotide binding sites on Rho hexamer. The binding of the first nucleotide to the Rho-RNA complex occurs at a bimolecular rate of 3.6${\times}$10$\^$6/ M$\^$-1/ sec$\^$-1/ whereas the second nucleotide binds at a slower rate of 4.7${\times}$10$\^$5/ M$\^$-1/ sec$\^$-1/ at 18$^{\circ}C$, RNA complexed with Rho affects the kinetics of nucleotide interaction with the active sites through conformational changes to the Rho hexamer, allowing the incoming nucleotide to be more accessible to the sites. Adenine nucleotide binding and dissociation is more favorable when RNA is bound to Rho, whereas ATP binding and dissociation step in the absence of RNA occurs significantly slower, at a rate ∼70- and ∼40-fold slower than those observed with the Rho-RNA complex, respectively.

The linker connecting the tandem ubiquitin binding domains of RAP80 is critical for lysine 63-linked polyubiquitin-dependent binding activity

  • Cho, Hyun-Jung;Lee, Sang-Ho;Kim, Hong-Tae
    • BMB Reports
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    • v.42 no.11
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    • pp.764-768
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    • 2009
  • The tandem ubiquitin-interacting motif (UIM) domain located at the N-terminus of Receptor Associated Protein 80 (RAP80) plays a crucial role in ionizing radiation (IR)-induced DNA damage response. RAP80 translocates to sites of IR-induced DNA damage through interaction of its UIM domain with ubiquitinated H2A and Lys63-linked polyubiquitin chains. The exact mechanism, however, through which RAP80 associates with Lys63-linked polyubiquitin chains is not clear. Here, we show by in vitro GST-pull down assays that modifying the linker region between the tandem ubiquitin binding domains of RAP80 changes the binding affinity for Lys63-linked polyubiquitin chains and affects translocation to sites of DNA breaks. Based on these findings, we suggest that the length of the linker region between the tandem ubiquitin binding domains of RAP80 may be a key factor in the binding of RAP80 with Lys63-linked polyubiquitin chains as well as in the translocation of RAP80 to DNA break sites.

The Application of the Evaluation Models Towards the Internet Business Web Site Model (인터넷 비즈니스 웹사이트 모델별 평가모형의 적용)

  • Son, Dal-Ho;Lim, Jun-Sig
    • IE interfaces
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    • v.17 no.1
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    • pp.33-45
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    • 2004
  • In the web environment for general information seeking and business-to-customer and business-to-business electronic commerce, users are customers. Understanding their expectations and how they feel about the web sites they use is becoming a very serious concern. While the success of a company or an organization is dependent on many factors, its web site plays a central role. This paper described the use of WebQual2.0 to evaluate the various web sites of B2B and B2C. WebQual2.0 has been extended and refined to encompass an interaction-quality literature. Furthermore, this paper described the use of Kano model to evaluate the various web sites which classified by internet business types. The result showed that the inherent characteristics of B2B and B2C web site should be considered in initial site construction. Further, customers in a web domain do not regard all quality factors as equally important and the rankings of important quality factors differ from one web domain to another. This study was focused on the identifying the weights of web quality factors according to the each web site of the internet business types.

Crystal Structure of a Cyclopropane Sorption Complex of Dehydrated Fully $Ca^{2+}$-Exchanged Zeolite X

  • 최은영;김양;송성환
    • Bulletin of the Korean Chemical Society
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    • v.20 no.7
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    • pp.791-795
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    • 1999
  • The crystal structure of a cyclopropane sorption complex of dehydrated fully Ca (2+) -exchanged zeolite X, Ca46Si100Al92O384· 30C3H6 (a = 24.988(4) Å), has been determined by single-crystal X-ray diffraction techniques in the cubic space group Fd3 at 21(1)℃. The crystal was prepared by ion exchange in a flowing stream of 0.05M aqueous Ca(NO3)2 for four days, followed by dehydration at 460℃ and 2×10 (-6) Torr for two days, and exposure to 100 Torr of cyclopropane gas at 21(1)℃. The structure was determined in this atmosphere and refined to the final error indices R1 = 0.068 and R2 = 0.082, with 373 reflections for which I > 3σ (I). In this structure, Ca 2+ ions are located at two crystallographic sites. Sixteen Ca 2+ ions fill the octahedral sites I at the centers of the hexagonal prisms (Ca-O = 2.412(9)Å). The remaining 30 Ca 2+ ions are at sites Ⅱ; each extends 0.46Å into the supercage (an increase of 0.16Å upon C3H6 sorption) where it coordinates to three trigonally arranged framework oxygens at 2.311(8)Å. Each of the 30 cyclopropane molecules was found to complex to Ca 2+ ions at site II by the induced dipole interaction (Ca-C = 2.99(4)Å). All carbon atoms in each cyclopropane molecule are equivalent and equidistant from Ca 2+ ions at site II with which they are associated.