• Molecules and Cells
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• 제39권2호
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• pp.77-86
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• 2016

Cancer is a heterogeneous disease caused by diverse genomic alterations in oncogenes and tumor suppressor genes. Despite recent advances in high-throughput sequencing technologies and development of targeted therapies, novel cancer drug development is limited due to the high attrition rate from clinical studies. Patient-derived xenografts (PDX), which are established by the transfer of patient tumors into immunodeficient mice, serve as a platform for co-clinical trials by enabling the integration of clinical data, genomic profiles, and drug responsiveness data to determine precisely targeted therapies. PDX models retain many of the key characteristics of patients' tumors including histology, genomic signature, cellular heterogeneity, and drug responsiveness. These models can also be applied to the development of biomarkers for drug responsiveness and personalized drug selection. This review summarizes our current knowledge of this field, including methodologic aspects, applications in drug development, challenges and limitations, and utilization for precision cancer medicine.

• 한국의류학회지
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• 제20권5호
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• pp.893-904
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• 1996

The main purpose of this research was to examine the purchase and use behavior of consumers related to washing machines in order to develope strategies to increase competitiveness of washing machines manufactured in Korea. Purchase and use behavior related to washing machines were examined by survey method and detailed information on washing conditions and usage of washing machines were obtained by diary method. 398 questionnaires were analyzed in the survey and 35 housewives participated in keeping the records of their washings for 30 days. The results of the survey showed that price and a tangle of clothes were the most determinant factors for purchase of washing machines and the knowledge related to washing was relatively low The diary method revealed that washing loads and frequencies of washing increase with the household size, and functions of washing machines except water level are infrequently used. Comparisons between the survey and diary results indicated that consistency between the two methods depends on the types of questions.

• 한국IT서비스학회지
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• 제16권3호
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• pp.1-15
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• 2017

This study is aimed to find out which factors among firm-specific resources, industry-based competition, and institutional conditions affect strategies for Korean IT manufacturing companies when they expand their business overseas, especially to Vietnam in this study. Also, it attempts to reveal which strategies among cost reduction, mass production, and localization affect performance of overseas expansion. Based on Strategy Tripod Theory as an integrating point-of-view embracing resource-based view, industry-based view, and institution-based view, research model was developed and tested through the survey of 83 Korean IT manufacturing companies expanded their business to Vietnam. Research findings show that (1) communication competency, industry competitiveness, industry attractiveness, and regulation of partner country affect cost reduction strategy. (2) International experience, communication competency, international orientation, and competitiveness is significantly associated with mass production strategy. (3) And international experience, communication competency, and industry competitiveness affect localization. (4) Ultimately, mass production strategy and localization strategy significantly increase performance of expansion to Vietnam. The theoretical contribution of this study is to adopt an integrative approach based on strategy tripod theory. As practical implications, it suggests factors and strategies that IT manufacturing companies interested in expansion to Vietnam should consider. In sum, we believe this study can contribute to Korean IT manufacturing industry by suggesting key success factors and appropriate strategies for overseas expansion.

• KSBB Journal
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• 제32권1호
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• pp.29-34
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• 2017

The completion of the Human Genome Project that identified all 3 billion base pairs in the human genome can be seen as a step towards understanding the relay of information and intention within an organism, or in other words, the language of life. The faculty of human language, key to differentiating humans from other animate species, works for conveying information to others by mapping meaning to sound based on syntactic structures. This resemblance between life and language has not gone unnoticed; the literature on RNA transcription and translation research regularly uses linguistic metaphors and the biolinguistic perspective of language has also been studied. By examining the biological characteristics of language and the linguistic characteristics of life, this study aims to identify key mechanisms shared between the two systems in order to promote a stronger connection between them. It furthers this goal by pointing out two general messages to which these mechanisms aim, productivity and accuracy, and discovers what lesson these messages give to a human society geared for sustainability.

• Animal cells and systems
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• 제15권3호
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• pp.251-258
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• 2011

Ciliates are considered one of the most diverse protozoa and play significant roles in ecology. For successful taxonomic study of these microscopic eukaryotes, a staining procedure is necessary, due mainly to intrinsic difficulties in recognizing characteristics from living cells. Although molecular taxonomy has been used to resolve the ambiguities associated with traditional morphology-based taxonomy, extraction of genomic DNA from stained ciliate cells is not available yet. In the present study, we describe a method to extract genomic DNA from a single protargol-impregnated euplotid cell. By using $HgCl_2$ as a fixative and modulating the exposure time of bleach solution in the protargol impregnation, high-quality genomic DNA can successfully be extracted from a stained single cell with minimal loss of morphological integrity. This technique will contribute to the effectiveness of combined approaches of molecular and morphological taxonomy from single ciliate cells.

• 한국동물학회지
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• 제33권3호
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• pp.266-275
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• 1990

Onocogene의 일종인 c-raf protein kinase는 세포질 속에 존재하는 serine / threonine-speccific protein이며, 이것은 mitogene signal에 의해 활성화된다. c-raf protein kinase의 구조와 기능은 protein kinase C와 매우 유사한 것으로 생각된다. 면역세포화학적으로 c-raf protein kinase의 signal transduction을 조사하기 위하여 EC-4 세포에 tumor promotor인 12-0-tet-radecanoylphorbol-13-acetae와 mitogenic gactor인 platelet-derived growth factor로 time-course에 따라서 처리하였다. Translocotion되는 c-raf는 먼저 perinuclear membrane에 모이고 그 후에 핵내로 이동되었다. 그런데 TPA와 PDGF로 처리한 c-raf의 translocotion은 각각의 다른 경로를 가짐을 알 수 있었다. TPA와 PAGF을 장기간 처리하였을 때, c-raf protein kinase의 down regulation이 유도됨을 알 수 있었다.

• 한국동물학회지
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• 제33권3호
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• pp.303-309
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• 1990

대장균에서 진핵세포의 펩타이드 호르몬 전구체를 대량으로 생산할 목적으로 T7 overexpression system을 이용하여 angler 어류의 prepro-SRIF I 유전자와 T7 phage coat 단백질 S10 유전자를 결합시켜 일련의 융합유전자를 합성하였다. 이 융합유전자를 가지고 있는 숙주 대장균, BL21 DE3는 3가지 종류의 서로 다른 SRIF 관련 폴리펩타이드를 대량 합성하였다. 본 연구에서는 대량합성된 폴리펩타이드의 특성을 규명하였으며, 펩타이드 호르몬 전구체를 얻기 어려운 이종이에서의 대량발현의 중요성과 표적 펩타이드 호르몬인 SRIF의 적용성을 논의하였다.

• Animal cells and systems
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• 제16권2호
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• pp.121-126
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• 2012

In this study, a neural network (NN) modeling approach has been used to predict the mechanical and geometrical behaviors of mouse embryo cells. Two NN models have been implemented. In the first NN model dimple depth (w), dimple radius (a) and radius of the semi-circular curved surface of the cell (R) were used as inputs of the model while indentation force (f) was considered as output. In the second NN model, indentation force (f), dimple radius (a) and radius of the semi-circular curved surface of the cell (R) were considered as inputs of the model and dimple depth was predicted as the output of the model. In addition, sensitivity analysis has been carried out to investigate the influence of the significance of input parameters on the mechanical behavior of mouse embryos. Experimental data deduced by Fl$\ddot{u}$ckiger (2004) were collected to obtain training and test data for the NN. The results of these investigations show that the correlation values of the test and training data sets are between 0.9988 and 1.0000, and are in good agreement with the experimental observations.

• The Journal of Asian Finance, Economics and Business
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• 제7권10호
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• pp.1015-1024
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• 2020

This inductive study seeks to establish a conceptual background for theoretical development regarding talent misuse in the context of small and medium-sized enterprises (SME). The two objectives for the goal were (1) to explore and build a list of unethical treatment practiced on young talents; (2) to establish new framework for the special context or refining the existing framework on employee mistreatment. For the first goal, an attempt was made to develop a list of mistreating behavior by collecting and analyzing cases of ethical disputes. Analyses on 64 cases of talent mistreatment, namely 'passion pay', was conducted to present a conceptual ground for further investigation. Then, for the second objective, this study has proposed an integrative approach for assessing these ethical elements. The ethical implications were discussed based on an assessment conducted using three theories of ethics. Finally, a cluster analysis further shows the emergence of three groups based on the mistreating behaviors. We observed seven different mistreatment behaviors under four categories of mistreatment practices. Additionally, the cluster analysis results imply that talent mistreatment may be rooted in work characteristics. Organizational size may provide some contextual reasoning, but the extent to which this factor interacts with work characteristics left unclear.

• 디지털융복합연구
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• 제12권5호
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• pp.479-490
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• 2014

본 연구는 보호관찰 청소년들의 대표적인 특성으로 비행의 원인이라고 할 수 있는 낮은 자기효능감 향상을 위한 차문화치료 프로그램을 개발하고 실제 적용을 통해 청소년들이 프로그램 참여 전후에 자기효능감에 어떤 변화과정을 보이는지 검증하였다. 프로그램의 효과성 검증을 위하여 양적 분석 및 질적 분석을 병행한 통합적 접근방식이 실행되었다. 보호관찰 청소년의 자기효능감 향상을 위한 차문화치료 프로그램에서 체계적 관찰을 통해 이루어진 질적 분석 결과, 청소년들의 태도와 적극적 참여 정도 부분이 크게 향상되었다. 또한 양적 분석 결과, 일반적 자기효능감과 사회적 자기효능감의 하위 요인에서 통계적으로 유의미한 향상을 보이는 것으로 나타났다.