Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Single-cell PCR on protargol-impregnated euplotid ciliates: a combined approach of morphological and molecular taxonomy

  • Kim, Se-Joo (Deep-Sea and Marine Georesources Research Department, Korea Ocean Research and Development Institute) ;
  • Choi, Joong-Ki (Department of Oceanography, Inha University) ;
  • Ryu, Seong-Ho (Department of Cell and Developmental Biology, Weill Medical College of Cornell University) ;
  • Min, Gi-Sik (Department of Biological Sciences, Inha University)
  • Received : 2010.11.11
  • Accepted : 2011.02.04
  • Published : 2011.09.30
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Abstract

Ciliates are considered one of the most diverse protozoa and play significant roles in ecology. For successful taxonomic study of these microscopic eukaryotes, a staining procedure is necessary, due mainly to intrinsic difficulties in recognizing characteristics from living cells. Although molecular taxonomy has been used to resolve the ambiguities associated with traditional morphology-based taxonomy, extraction of genomic DNA from stained ciliate cells is not available yet. In the present study, we describe a method to extract genomic DNA from a single protargol-impregnated euplotid cell. By using $HgCl_2$ as a fixative and modulating the exposure time of bleach solution in the protargol impregnation, high-quality genomic DNA can successfully be extracted from a stained single cell with minimal loss of morphological integrity. This technique will contribute to the effectiveness of combined approaches of molecular and morphological taxonomy from single ciliate cells.

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References

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