• 해운물류연구
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• 제34권4호
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• pp.545-563
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• 2018

본 연구의 목적은 VLCC(Very Large Crude Oil Carrier) 운임에 영향을 미치는 주요 결정요인의 장기적 탄성치를 추정하는 것이다. 이를 위해 본 연구는 영국 해운 전문 기업인 클락슨이 공표하는 연간 VLCC 운임을 종속변수로, 원유(Crude oil) 물동량, VLCC 선복량, 벙커유 가격, Libor 금리를 설명변수로 사용하였다. 본 연구는 벡터오차수정모형(Vector Error Correction Model; VECM)을 사용하여 운임 결정 장기균형함수를 추정하였으며, 추정결과 물동량 1.0% 증가 시 운임 6.4% 증가, 선복량 1.0% 증가 시 운임 1.9% 감소, 벙커유 가격 1.0% 증가 시 운임 0.3% 감소, 금리 1.0% 증가 시 운임은 0.18% 증가하는 것으로 나타났다. 벙커유 가격의 경우 일반적인 직관과 반대되는 마이너스(-) 부호로 계수가 추정되었는데, 이는 설명변수 중 벙커유 가격이나 금리 등의 2차 변수가 운임에 미치는 영향력은 적은 반면 직접적인 수급 변수가 운임을 결정하는 주요 요인이기 때문인 것으로 이해된다. 후속연구에서 컨테이너선, 건화물선 등 다른 선종들을 대상으로 연구를 수행하고 다양한 선종별 운임의 결정요인을 비교 분석하는 것이 필요하다.

• 지역연구
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• 제35권1호
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• pp.47-57
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• 2019

본 연구의 목적은 계량분석을 통해 우리나라 지역내총생산에 영향을 미치는 주요 요인별 영향력을 분석하는 것이다. 본 연구는 분석대상 지역으로 대구광역시를 선정했는데 대구광역시는 영남 지역의 중추기능을 지속적으로 수행해 왔으며, 우리나라 섬유 화학제품 등의 수출 전략기지로 지위하고 있다. 또한 영남 지역에 도달하는 주요 수입화물의 기종점 역할을 수행하는 등 이처럼 대구 지역은 우리나라 수출입 무역 확대와 국가경제 성장에 기여하는 바가 높다. 이를 위해 본 연구는 공적분모형(Co-integration Model)과 벡터오차수정모형(Vector Error Correction Model; VECM)을 사용하여 대구 지역내총생산에 영향을 미치는 장기균형함수를 추정하였다. 본 연구는 우리나라 지역내총생산에 영향을 미치는 주요 요인들의 영향력을 정량적인 방식을 통해 추정하고 장기 균형 시점의 총생산으로부터 괴리가 발생했을 때 얼마나 빠른 속도로 장기균형으로 수렴하는가를 추정하였다는 점에서 의의가 있다.

• 한국항공우주학회지
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• 제50권1호
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• pp.67-74
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• 2022

일반적으로 동역학 방정식을 이용하여 항공기의 거동을 분석하기 위해서는 공기 역학적 성분이 필요하고, 이를 위해서는 진 대기속도가 필수적이다. 대기속도는 피토관과 같은 항공기의 탑재 장비를 이용하여 계측할 수 있으나, 상업용 항공기의 계측 데이터는 항공사 내규 관련 보안성 데이터를 포함할 수 있으므로 확보하기 어렵다. 일반인들이 이용할 수 있는 항적 데이터 중 하나로 방송형자동종속감시 데이터가 있으며 항공기의 속도를 대지속도 형태로 제공한다. 대지속도는 진 대기속도와 바람 속도의 합으로 표현된다. 본 논문에서는 일반인들이 획득 가능한 항적, 기상, 지형 데이터를 결합하여 진 대기속도를 추정하는 방법을 제시한다. 각 데이터를 통합하기 위해 좌표계를 일치시킨 후 고도 기준을 평균 해수면 고도로 통일하였다. 또한, 해상도가 가장 낮은 기상 데이터의 격자를 기준으로 보간을 수행하여 항적 데이터의 모든 점에 대한 바람 벡터를 산출하였다. 이러한 과정을 여러 항적에 적용하여 기상, 지형 데이터의 통합을 통해 진 대기속도를 추정하였다.

• Molecules and Cells
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• 제46권4호
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• pp.209-218
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• 2023

In induced pluripotent stem cells (iPSCs), pluripotency is induced artificially by introducing the transcription factors Oct4, Sox2, Klf4, and c-Myc. When a transgene is introduced using a viral vector, the transgene may be integrated into the host genome and cause a mutation and cancer. No integration occurs when an episomal vector is used, but this method has a limitation in that remnants of the virus or vector remain in the cell, which limits the use of such iPSCs in therapeutic applications. Chemical reprogramming, which relies on treatment with small-molecule compounds to induce pluripotency, can overcome this problem. In this method, reprogramming is induced according to the gene expression pattern of extra-embryonic endoderm (XEN) cells, which are used as an intermediate stage in pluripotency induction. Therefore, iPSCs can be induced only from established XEN cells. We induced XEN cells using small molecules that modulate a signaling pathway and affect epigenetic modifications, and devised a culture method which can produce homogeneous XEN cells. At least 4 passages were required to establish morphologically homogeneous chemically induced XEN (CiXEN) cells, whose properties were similar to those of XEN cells, as revealed through cellular and molecular characterization. Chemically iPSCs derived from CiXEN cells showed characteristics similar to those of mouse embryonic stem cells. Our results show that the homogeneity of CiXEN cells is critical for the efficient induction of pluripotency by chemicals.

• Asian-Australasian Journal of Animal Sciences
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• 제27권11호
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• pp.1644-1651
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• 2014

Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine ${\beta}$-casein gene locus using a knock-in vector for the ${\beta}$-casein gene locus. We developed the knock-in vector on the porcine ${\beta}$-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5' arm and 3' arm from the porcine ${\beta}$-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using ${\beta}$-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous ${\beta}$-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.225-231
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• 2005

Five day old cotyledon explants of Cucumber (Cucumis sativus L) cv Poinsett 76 were cocultivated with two Agrobacterium strains (EHA105 and LBA 4404) each carrying GUS as the reporter gene and npt-II as the selection marker gene in the T-DNA region of the vector. Transformed shoots were selected at 150 mg/L kanamycin. A two day cocultivation coupled with $20\;{\mu}M$ acetosyringone increased the frequency (8.2 and 15.4 shoots) of GUS expression in the shoots of transformed plant. Among the two Agrobacterium strains, EHA 105 performed better than LBA 4404 in bringing two-fold increase in transformation efficiency (14%) than LBA 4404 (7.4%). PCR analysis was done to confirm the integration of T-DNA into cucumber genome.

• 소성∙가공
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• 제21권7호
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• pp.441-446
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• 2012

A sequential coupled field analysis of electromagnetic free bulging was performed by using FEM. A 2D axi-symmetric electromagnetic model based on the magnetic vector potential is proposed for the calculation of magnetic field and Lorentz's forces. The Newmark integration method is used to calculate the transient dynamic plastic deformation of sheet during free bulging. In the finite element model, the effect of sheet deformation on the electromagnetic field analysis is taken into consideration. In order to confirm the sequential electromagnetic-mechanical coupling analysis, an experiment with an electromagnetic forming apparatus was conducted. The results showed that the final bulge height of the sheet predicted from the proposed method is in good agreement with experimentally measured height.

• 한국정밀공학회지
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• 제17권12호
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• pp.201-208
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• 2000

A numerical analysis algorithm for thermally loaded structures has been proposed and compared with the general free vibration approach to determine the characteristics of thermal load effects in vibration structures. The field of numerical inspection includes free vibration analysis, transient heat transfer analysis and thermal stress analysis. The key point of the analysis of thermally loaded structure is the method of parallel time integration between transient heat transfer and free vibration simultaneously. The results of the study demonstrate the computation of the specific total external force vector and stiffness matrix. The proposed analysis method can be applied to both heated and cooled structure vibration analysis.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.49-52
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• 2002

High-resolution satellite image processing software should be able to ensure accurate, fast, compact data processing in offline or online environment. In this paper, component software for high-resolution satellite image processing is developed using OpenGIS components and real-time data processing architecture. The developed component software is composed of three major packages, which are data provide package, user interface package, and fast data processing package. The data provider package encodes and decodes diverse image/vector data formats and give identical data access methods to developers. The user interface package supports menus, toolbars, dialogs, and events to use easier. The fast data processing package follows the OpenGIS's data processing standards, which can deal with several processors as components with standard procedural functionalities.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2003년도 춘계학술대회논문집
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• pp.283-287
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• 2003

In recent years, design of an optimal blank shape is very important for sheet metal forming process in the automobile industry because the raw material cost rate is significant part in the automobile industry. With the design of an optimal blank shape, the engineer can protect a blank from an excessive holding force to improve the quality and reduce the ratio of material scrap. Therefore design of an optimal blank shape is inevitable in sheet metal forming process. However, if it causes a complicated shape of blank, it may be difficult to do the blank layout optimally. In this study, we developed software of optimal blank layout connected with the software of optimal blank shape design which was created in the past by the present authors. And by using these softwares, we would like to present the method in order to get optimal utilization ratio easily and precisely within short time for the sequence of works from design to blank layout.