• 한국발생생물학회지:발생과생식
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• 제21권3호
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• pp.229-235
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• 2017

In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved ($17^{\circ}C$ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from $11.0{\pm}2.3beats/s$ (fresh sperm) to $5.7{\pm}1.8beats/s$ and increased the flagellar bending angle from $19.8^{\circ}{\pm}13.8^{\circ}$ (fresh) to $30.6^{\circ}{\pm}15.6^{\circ}$. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques.

• 한국임상수의학회지
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• 제26권4호
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• pp.376-378
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• 2009

2007년 8월 26일 생인 7개월된 숫송아지에게 음경전위술을 실시했다. 음경의 방향을 바꿔 정상적인 교미를 불가능하게 함으로써 경산우와 처녀우군에 도입이 되면 후각을 이용하여 발정을 탐지하게 되고 수의사에게 인공수정 혹은 수정란이식의 최적시간을 제공하게 될 것이다. 본 보고는 어린 숫송아지에게 실시되는 음경전위술법을 설명한 것이다.

• 한국가축번식학회지
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• 제13권2호
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• pp.70-78
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• 1989

Mouse eggs recovered from oviducts at one hourly intervals between 10 and 20 hours after administration of hCG were fixed, stained and then investigated the rate of in vitro fertilization and nuclear maturation. In case of out- bred ICR mice, ovulations were occured between 11 and 13 hours after hCG injection. The stages of in vitro maturation of eggs recovered from female mice at various times after hCG injection were metaphase I, anaphase I, telophase I and metaphase II. However the majority was metaphase I(17.6 to 44.4%) and metaphase II(42.9 to 80.0%) stage. When the eggs were inseminated with epididymal spermatozoa, the fertilization rate was declined as the egg recovery time after hCG administration was delayed. That is, the proportion of eggs undergoing fertilization became higher(68.1 to 77.4%) in the eggs at 12 to 15hr after injection of hCG than those(17.5 to 56.4) at 16 to 20 hr after injection of hCG. Also, when nuclear maturation of the unfertilized eggs were observed at 8 hours after insemination, the majority was in metaphase I and metaphase II and no anaphase I and telophase I were observed.

• 한국가축번식학회지
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• 제13권1호
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• pp.1-6
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• 1989

A field survey was conducted to obtain the data on reproductive performation of Korean native cattle. The data for this study were taken from 6,461 breeding records of cows raising at general farms in 8 Hanwoo(native cattle) pure breeding areas from June, 1984 through November, 1985. The recycling rate within 30 days, 31 to 60 days, and 61 to 90 days postpartum was 7.5%, 40.0%, and 32.8%, and it amounted to 80.3%, while the non-recyling rate up to 120days postcalving was 8.4%. Conception rate according to insemination(AI) frequencies was 65.7% at 1st AI, 21.3% at 2nd AI, and 8.4% at 3rd AI, respectively. It amounted to 95.4% up to 3rd AI. Clving rate during the spring time was the highest, 39.6%, and tended to be reduced according to summer(31.6%), autumn (16.3%), and winter (12.5%). Spring and summer were the highest seasons for pregnancy and their gestation rates are 33.7% in spring and 39.2% in winter, respectively. The days from calving to estrus and conception, and calving interval of the cows, which calved two or three times, tended to be reduced. However, the more parities, the more they are extended. According to parities, gestation length and services per conception showed irregular tendencies.

• 한국가축번식학회지
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• 제8권2호
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• pp.79-86
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• 1984

This cepseiment was carried out to cerify the effect of thawing methods and preservative temperature on the sperm motility and fertility after thawing semen with plastic straws in fresh and warm water. Sperm motility in vitro stored at room temperature after thawing were conducted by the various storage hours. A field trial after thawing semen with warmed water in straws from Cheju native cows involving 4 technicians and 800 cows first (or second) services gave the following results. The thawing methods of warmed water for one minute in semen motility were considerably higher than that in iced water during 12 hours after thawing semen, however, the sperm survival index of ice-water shwed a better results according as the time passed away, but not significant differences. Preservative temperaure at 5$^{\circ}C$ (iced water) after thawing gave significantly better results than that of thawed at 3$0^{\circ}C$ (warmed water). The N R rate to 175 inseminations with semen thawed at 15-2$0^{\circ}C$ (fresh water) was 82.8%, 80.9% for 610 inseminations thawed in warm water. Conception rate ofthe semen thawed in warm water for 10-60 secs gave no significant difference among storage hours, because the semen used to be inseminated within one hour almost, but in decreased when semen thawed at the period of one minute over.

• Clinical and Experimental Reproductive Medicine
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• 제3권2호
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• pp.29-34
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• 1976

동물에서 정상적인 임신유지를 함에 필요한 홀몬의 양을 측정하기 위하여 토끼에서 인공수정을 실시한 다음날 양쪽 ovary를 제거하고, 홀몬대치요법(replace therapy)에 의해서 인공적으로 임신상태를 분만시까지 유지시키면서 competitive protein-binding assay method을 이용하여 혈중 progestin의 농도를 측정하여 정상 임신토끼와 비교하였던 바 다음과 같은 결과를 얻었다. 1) 정상임신토끼에 있어서 혈중 progestin의 평균 농도 변화는 제 1일에 $1.7{\sim}2.7$ ng/ml으로 시작해서 차차 증가되어 제$13{\sim}15$일까지는 $19.8{\sim}25.3$ ng/ml까지 상승하였다가 그후 분만시까지 서서히 감소되었다. 2) 홀몬 대치요법에 의해서 인공적으로 임신을 유지시킨 토끼의 혈중 progestin 농도는 정상토끼의 progestin 농도의 변화와 매우 비슷한 변화곡선을 나타내었다.

• 한국수산과학회지
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• 제19권6호
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• pp.575-580
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• 1986

1986년초 저자들은 불임 3배체 무지개송어의 대량 생산을 위하여 수정난을 $27^{\circ}C$로 열처리하였다. 그 결과 $50\%$ 전후의 3배체 무지개송어가 유도되었으며 유도된 3배체 송어의 초기 성장률은 여타 보고들과 같이 정상 2배체와 유사하였다. 앞으로 본 방법을 수정개량하여 처리함으로써 산업적으로 중요한 불임 무지개송어의 생산성을 높일 수 있으리라 사료된다.

• 한국임상수의학회지
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• 제19권1호
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• pp.73-79
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• 2002

To investigate the usability of frozen canine embryos for embryo transfer in the dog, 19 donors, 3 recipients, and 6 male dogs were used for the experiment. Natural mating or artificial insemination was performed for breeding the bitches in natural estrus. Vaginal smear test along with progesterone titre test were performed to detect the appropriate mating time and the bitches were bred twice during 3-6days following LH surge. Embryo collection was done on 8, 9-11, 12-13 days after the second mating to collect morula and blastocyst. Embryos were frozen using a programmable freezer and preseued in LNE tank. Embryos were thawed in 37$^{\circ}C$ water for 15 seconds and transferred into each uterine horn within 30 minutes. Embryos were collected from 13 bitches of 19 donors(68.4%) and the collected embryos were from between 9 and 13 days after 2nd mating. Embryos were produced both by natural mating(60.0%, 9115) and AI with frozen semen(100.0%, 4/4). Embryos were collected from the donors weighed between 2.5 and 30 kg and their age was from 1.5 to 3 years. 52 embryos were collected from 13 donors and the mean number of embryos was four. The stage of embryos was from 2-cell to gastrula and morulae were colledted mostly from 10 to 11 days after 2nd mating. Embryos were collected evenly from each uterine horn and the rate of embryo collection for the number of corpus luteum was 83.9%. Embryos were transferred to 3 recipients(morula 8, blastocyst 1, gastrula 8), however, no offspring was produced.

• 한국임상수의학회지
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• 제24권2호
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• pp.142-149
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• 2007

The purpose of this study was to develop a computerized software program to help for reproductive management in dairy cows. The developed software program in the study is compatible with a window 95/98 or XP system. Data in the system were recorded, stored, and processed from two sources: 1) Data downloaded monthly from the database of the Korean Dairy Herd Improvement Association (milk yields, milk somatic cell counts, milk fat, protein, lactose and urea nitrogen content). 2) Data recorded by the farmer or veterinarians by the time (body condition score, heats, inseminations, veterinary diagnosis and treatments). These data indices after processing by computerized dairy management system were presented by numerical or graphical display. The presented data were obtained from three dairy farms with more than 50 milking cows. The presented reports of this program using milk fat, protein, urea nitrogen, and somatic cell counts enabled the dairy producer and veterinarians to monitor the protein-energy balance and feeding management practice, and for distribution of diseases (mastitis, metabolic and reproductive disorder) in individual cows. The presented analytical reports of this program also included herd average of reproductive indices such as day to first insemination, days open, and inseminations per conception. This software program will assist in analysis, interpretation and demonstrate the results of reproductive trials conducted in dairy herds.

• 한국동물생명공학회지
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• 제36권1호
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• pp.45-50
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• 2021

In recent years, various methods of measuring body temperature have been developed using wireless biosensors to facilitate an early detection of pregnancy and parturition in cows. However, there are no studies on real-time monitoring of cattle body temperature throughout pregnancy. Therefore, we investigated the daily mean ruminal temperature in pregnant cows throughout pregnancy using a ruminal bio-capsule sensor and then evaluated the temperature variation between pregnant and non-pregnant cows. In pregnant cows, the mean and standard deviation of ruminal temperature was 38.86 ± 0.17℃. Ruminal temperature in pregnant cows slowly decreased until 180 to 190 days after artificial insemination and after that, the temperature increased dramatically until just before parturition. Furthermore, the means ruminal temperature was significantly different between pregnant and nonpregnant cows. The mean and standard deviation of ruminal temperature were as follows: 38.68 ± 0.01℃ from days 80 to 100, 38.78 ± 0.02℃ from days 145 to 165, 38.99 ± 0.45℃ from days 200 to 220, 39.14 ± 0.38℃ from days 250 to 270 before parturition. Therefore, our results could provide useful data for early detection of pregnancy and parturition in Korean cows.