Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Embryo Transfer with frozen Embryos in the Dog

개에서 동결수정란의 이식

  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

To investigate the usability of frozen canine embryos for embryo transfer in the dog, 19 donors, 3 recipients, and 6 male dogs were used for the experiment. Natural mating or artificial insemination was performed for breeding the bitches in natural estrus. Vaginal smear test along with progesterone titre test were performed to detect the appropriate mating time and the bitches were bred twice during 3-6days following LH surge. Embryo collection was done on 8, 9-11, 12-13 days after the second mating to collect morula and blastocyst. Embryos were frozen using a programmable freezer and preseued in LNE tank. Embryos were thawed in 37$^{\circ}C$ water for 15 seconds and transferred into each uterine horn within 30 minutes. Embryos were collected from 13 bitches of 19 donors(68.4%) and the collected embryos were from between 9 and 13 days after 2nd mating. Embryos were produced both by natural mating(60.0%, 9115) and AI with frozen semen(100.0%, 4/4). Embryos were collected from the donors weighed between 2.5 and 30 kg and their age was from 1.5 to 3 years. 52 embryos were collected from 13 donors and the mean number of embryos was four. The stage of embryos was from 2-cell to gastrula and morulae were colledted mostly from 10 to 11 days after 2nd mating. Embryos were collected evenly from each uterine horn and the rate of embryo collection for the number of corpus luteum was 83.9%. Embryos were transferred to 3 recipients(morula 8, blastocyst 1, gastrula 8), however, no offspring was produced.

Keywords

References

  1. Vet.Med. Small Anim Clin v.75 A surgical method for collecting canine embryos after induction of estrus and ovulation with exogenous gonadotropins Archbald LF;Baker BA;Clooney LL(et al.)
  2. Am J Vet Res v.49 Use of pulsatile intravenous administration of gonadotropin - releasing hormone to induce fertile estrus in bitches Cain JL;Cain GR;Feldman EC(et al.)
  3. Current Veterinary therapy XE small animal pratice Induction of estrus and ovulation in the bitch Cain JL;Kirk RW(ed.);Bonagura JD(ed.)
  4. Proceedigns of the Amercan Society of Animal Science v.337 Hormone Levels during the estrous cycle, pregnancy and pseudopregnancy in the Labrador bitch(abstract) Chakraborty PK;Panko WB;Seager SWJ
  5. verterinary obstetrics and genital diseases(theriogenology) Embryo transfer in dogs and cats Drost M;Roberts SJ(ed.)
  6. Current therapy in theriogenology Induction estrus in the bitch. Evans LE;Morrow DA(ed.)
  7. Canine and feline endocrinology and reproductionl Feldman EC;Nelson RW
  8. Biol Reprod Suppl v.1 Surgical collection and transfer of canine embryos Kinney GM;Pennycook JW;Schriver MD(et al.)
  9. Current therapy in theriogenology 2 Embryo transfer in laboratory animals Kraemer. DC;Bowen MJ;Morrow DA(ed.)
  10. Theriogenology v.11 Embryo transfer in the non-human primate, feline and canine Kraemer DC;Flow BI;Schriver MD(et al.) https://doi.org/10.1016/S0093-691X(79)80018-7
  11. Veterinary anesthetia(2nd ed.) Lumb WV;Jones EW
  12. Jpn. J. Vet. Sci v.47 no.1 Induction of estrus in bitches with exogenous gonadotropins, and pregnancy rate and blood progesterone profils Nakao T;Aoto Y;fukushima S(et al.) https://doi.org/10.1292/jvms1939.47.17
  13. Vetrinary obsterics and genital disease(thero-genology) Roberts. SJ
  14. JAVMA v.184 Efforts to induce estrus in the bitch, using pituitary gonadotropins Shillie VM;Thatcher MJ;Simmons KJ
  15. Jpn J Anim Reprod v.26 Studies on the reproduction in the dog. A trial of ova transplantation in dogs Takeishi M;Akai R;Tsunekane T(et al.) https://doi.org/10.1262/jrd1977.26.151
  16. Am J Vet Res v.38 Induction of estrus and ovulation in the bitch, using exogenous gonadotropins Thun R;Watson P;Jackson GL
  17. Jpn.J.Vet.Sci. v.51 Effects of prostaglandin F2 α -analogue administration on luteal function, implantation of embryos and maintenance of pregnancy in bitches Tsutsui T;Kasakami E;Orima H(et al.) https://doi.org/10.1292/jvms1939.51.496
  18. Jpn.J.Vet.Sci v.51 Embryo transfer in the cat during the non-breeding Season Tsutsui T;Sato M;Kurosawa N(et al.) https://doi.org/10.1292/jvms1939.51.871
  19. Jpn.J.Vet.Sci v.51 An experimental trial on embryo transfer in the dog Tsutsui T;Shimada K;Nichi M(et al.) https://doi.org/10.1292/jvms1939.51.797
  20. Jpn.J.Vet.Sci v.44 Effects of prostaglandin F2 α on implantation and maintenance of pregnancy in the dog Tsutsui T;Takatani H;Hirose O(et al.) https://doi.org/10.1292/jvms1939.44.403
  21. Vet Rec v.135 no.23 A new method of paternity testing for dog, based on miocrosatellite sequences Zajc I;Mellersh C;kelly EP;Sampson J
  22. 대한수의학회지 v.34 no.2 자연발정견 및 인공발정 유도견에서 수정란이식 김용준