• Title/Summary/Keyword: initial cell density

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Nitrogen removal from wastewaters without carbon sources using microalgae

  • Lee, Kwang-Yong;Lee, Choul-Gyun
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.553-556
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    • 2000
  • Possibility of biological nitrogen treatment was tested in wastewaters with low C/N ratio. Chlorella kessleri was inoculated at $10^6\;cell/mL$ of initial density in two different artificial wastewaters: one that contained glucose for organic carbon source and the other without carbon source. Nitrate could be successfully reduced below 10 mg $NO_3/mL$ from initial nitrate concentration of 560 mg $NO_3/mL$ in 10 days even in the wastewater without carbon source, This 98% removal of nitrate without extra organic carbon source lights up the future of biological wastewater treatment, where the insufficient ability of nitrogen removal is a major problem.

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Hydrogen photoproduction by the synchronously grown marine unicellular cyanobacterium Synechococcus sp. Miami BG 043511 under extremely high oxygen concentration

  • Yih, Won-Ho;Takeyama, Haruko;Mitsui, Akira
    • Journal of the korean society of oceanography
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    • v.31 no.1
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    • pp.18-22
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    • 1996
  • The effect of exogenous oxygen on hydrogen photoproduction was examined in the synchronously grown cells of marine Synechococcus sp. Miami BG 043511 under conditions of high cell density (0.6-0.8 mg chl-${\alpha}$ $ml^{-1}$) and high light intensity (1000 ${\mu}$E $m^{-2}$ $s^{-1}$). Hydrogen evolution after 20-h incubation did not decline under the initial oxygen concentrations up to 20%, but declined by half under 34% oxygen. 50% and 100% oxygen gas phase did not completely inhibit the hydrogen photoproduction during 40-h incubations. After 2-day pretreatment under 100% exogenous oxygen the hydrogen photoproduction capabilities were not irreversibly inhibited, which was demonstrated in the subsequent 9-day incubation under initial 0, 50 and even under 100% oxygen gas phase. This strain could be useful for developing a hydrogen photoproduction system under atmospheric oxygen concentration.

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Improvement of Power Generation of Microbial Fuel Cells using Maximum Power Point Tracking (MPPT) and Automatic Load Control Algorithm (최대전력점추적방법과 외부저항 제어 알고리즘을 이용한 미생물연료 전지의 전력생산 최대화)

  • Song, Young Eun;Kim, Jung Rae
    • KSBB Journal
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    • v.29 no.4
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    • pp.225-231
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    • 2014
  • A microbial fuel cell (MFC) and bioelectrochemical systems are novel bioprocesses which employ exoelectrogenic biofilm on electrode as a biocatalyst for electricity generation and various useful chemical production. Previous reports show that electrogenic biofilms of MFCs are time varying systems and dynamically interactive with the electrically conductive media (carbon paper as terminal electron acceptor). It has been reported that maximum power point tracking (MPPT) method can automatically control load by algorithm so that increase power generation and columbic efficiency. In this study, we developed logic based control strategy for external load resistance by using $LabVIEW^{TM}$ which increases the power production with using flat-plate MFCs and MPPT circuit board. The flat-plate MFCs inoculated with anaerobic digester sludge were stabilized with fixed external resistance from $1000{\Omega}$ to $100{\Omega}$. Automatic load control with MPPT started load from $52{\Omega}$ during 120 hours of operation. MPPT control strategy increased approximately 2.7 times of power production and power density (1.95 mW and $13.02mW/m^3$) compared to the initial values before application of MPPT (0.72 mW and $4.79mW/m^3$).

Effect of Lactobacillus acidophilus based probiotic product supplementation on the blood profile, fecal noxious gas emission, and fecal shedding of lactic acid bacteria and coliform bacteria in healthy adult Beagle dogs

  • Sun, Hao Yang;Kim, In Ho
    • Korean Journal of Agricultural Science
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    • v.47 no.3
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    • pp.437-443
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    • 2020
  • The aim of this study was to evaluate the effect of Lactobacillus acidophilus probiotic (LAP) product supplementation on the blood profile, fecal noxious gas emission, and fecal shedding of lactic acid bacteria and coliform bacteria in healthy adult Beagle dogs. In total, 14 Beagle dogs with an average initial body weight of 10.19 ± 0.61 kg were randomly assigned into two dietary treatments,with and without LAP supplementation, for a 28-day feeding trial. At the end of the experiment, there was no significant (p > 0.05) difference in the concentration of serum total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), white blood cell (WBC), red blood cell (RBC), blood lymphocyte percentage, fecal hydrogen sulfide (H2S) and total mercaptans (R.SH) emission, and fecal coliforms counts. However, the serum concentrations of the triglyceride and fecal ammonia (NH3) emission of the LAP treatment were significantly (p < 0.05) decreased in the group compared with the CON dogs. Fecal total lactic acid bacteria counts were significantly (p < 0.05) increased in the LAP treatment. In conclusion, the supplementation of LAP in Beagle dog diets could decrease the blood triglyceride level and enhance the gut Lactobacillus count which may have positive effects on dogs.

The electrochemical Characteristics on the Anode Material of Lithium Ion Secondary Batteries with Discharge Voltage (방전전압에 따른 리튬 이온 2차전지용 음극물질의 전기화학적 특성)

  • Park, Jong-Gwang;Han, Tae-Hui;Jeong, Dong-Cheol;Im, Seong-Hun;Han, Byeong-Seong
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.49 no.6
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    • pp.328-334
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    • 2000
  • A lithium ion secondary battery using carbon as a negative electrode has been developed. Further improvements to increase the cell capacity are expected by modifying the structure of the carbonaceous material. There are hopes for the development of large capacity lithium ion secondary batteries with long cycle, high energy density, high power density, and high energy efficiency. In the present paper, needle cokes from petroleum were examined as an anode of lithium ion secondary battery. Petroleum cokes, MCL(Molten Caustic Leaching) treated in Korea Institute Energy Research, were carbonized at various temperatures of 0, 500, 700, $19700^{\circ}C$ at heating rate of $2^{\circ}C$/min for lh. The electrolyte was used lM liPF6 EC/DEC (1:1). The voltage range of charge & discharge was 0.0V(0.05V) ~ 2.0V. The treated petroleum coke at $700^{\circ}C$ had an initial capacity over 560mAh.g which beyond the theoretical maximum capacity, 372mAh/g for LiC6. This phenomena suggests that carbon materials with disordered structure had higher cell capacity than that the graphitic carbon materials.

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A Prediction of Chip Quality using OPTICS (Ordering Points to Identify the Clustering Structure)-based Feature Extraction at the Cell Level (셀 레벨에서의 OPTICS 기반 특질 추출을 이용한 칩 품질 예측)

  • Kim, Ki Hyun;Baek, Jun Geol
    • Journal of Korean Institute of Industrial Engineers
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    • v.40 no.3
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    • pp.257-266
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    • 2014
  • The semiconductor manufacturing industry is managed by a number of parameters from the FAB which is the initial step of production to package test which is the final step of production. Various methods for prediction for the quality and yield are required to reduce the production costs caused by a complicated manufacturing process. In order to increase the accuracy of quality prediction, we have to extract the significant features from the large amount of data. In this study, we propose the method for extracting feature from the cell level data of probe test process using OPTICS which is one of the density-based clustering to improve the prediction accuracy of the quality of the assembled chips that will be placed in a package test. Two features extracted by using OPTICS are used as input variables of quality prediction model because of having position information of the cell defect. The package test progress for chips classified to the correct quality grade by performing the improved prediction method is expected to bring the effect of reducing production costs.

A Study of Pseudomonas putida Fed-batch Culture (Pseudomonas putida의 유가배양연구)

  • 김인호;김희정;송재양
    • KSBB Journal
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    • v.17 no.3
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    • pp.307-310
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    • 2002
  • In order to obtain high density seed cells for biofiltration, we studied batch and fed-batch culture of P. putida. Studies were carried out to find optimum fermentation conditions such as pH, concentration of glucose and agitation speed. Specific growth rate of P. putida was dependent on agitation speed and a high rpm of 300 was necessary to carry out the efficient aerobic growth of P. putida. Specific growth rate was highest at pH 7. Feeding glucose and yeast extract continuously at the initial growth phase was the most effective way to get high cell density of P. putida.

Analysis of the Bone-remodeling Process Considering Stimuli Delivery Cell Model (자극전달세포 모델을 고려한 골 재형성 해석)

  • Moon Hee-Wook;Kim Young-Eun
    • Journal of the Korean Society for Precision Engineering
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    • v.23 no.6 s.183
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    • pp.180-186
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    • 2006
  • To investigate the bone remodeling phenomenon around implant device, 3-D mathematical simulation model was developed. Strain energy density from the finite element method was chosen for the indicator for remodeling process. Recursive calculations continued until converged results between FEM and mathematical model. For a osteo-integration example, bone-remodeling process in a implanted tibia of beagle was adapted. Calculated results indicated that the bone densities around screw pitch were increased which indicates firm fixations between the bone and implant. Screw design parameters have an influence on initial stability of the implant rather than remodeling process.

Removal of Cochlodinium polykrikoides using a novel material produced from sediment - A field study

  • Song, Young-Chae;Sivakumar, S.;Ko, Sung-Chung;Hwang, Eung-Ju;Jo, Q-Tae
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2007.12a
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    • pp.200-201
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    • 2007
  • The present study was conducted in three fields at Namhae to examine the removal efficiency of organic free, heavy metal immobilized sediment on Cochlodinium polykrikoidesand and on sea water quality. The present study results concluded that removal efficiency was depends on the initial number of red tide cells. There was no drastic change in the sea water quality after sediment spray. For the comparison of effectiveness of betonite, zeolite and lime were mixed with sediment did not show any marked difference in removal. Finally, the present study evaluated 50g/$m^{2}$ sediment is sufficient to remove 100% cells density, even though various environmental factors are interfering the mechanism.

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High Density Cultivation of Methylobacillus sp. SK1 in Fed-Batch System (Methylobacillus sp. SK1의 고농도 유가배양)

  • 이형춘;이계호김시욱
    • KSBB Journal
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    • v.5 no.3
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    • pp.269-277
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    • 1990
  • Methylobacillus sp. SK1, an obligate methylotroph, was cultivated in a fed-batch culture using DO as a methanol feeding indicator with a micro computer-aided control system. While 2.07g/l of cell density was obtained after 13 hr in the batch culture (initial methanol concentration: 1.0%(v/v)),45.3g/l of cell density was obtained after 17 hr by feeding methanol and metal ions in the fed-batch culture with oxygen supply. The high-density biomass was obtained in short cultuivation time by fed-batch culture with feedback control, and consequently the biomass productivity was significantly increased. It was mainly due to extension of logarithmic growth period by methanol feeding without methanol inhibition and intensive aeration without DO limitation with microcomputer-aided control system.

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