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A Study of Pseudomonas putida Fed-batch Culture  

김인호 (충남대학교 화학공학과)
김희정 (충남대학교 화학공학과)
송재양 (충남대학교 화학공학과)
Publication Information
KSBB Journal / v.17, no.3, 2002 , pp. 307-310 More about this Journal
Abstract
In order to obtain high density seed cells for biofiltration, we studied batch and fed-batch culture of P. putida. Studies were carried out to find optimum fermentation conditions such as pH, concentration of glucose and agitation speed. Specific growth rate of P. putida was dependent on agitation speed and a high rpm of 300 was necessary to carry out the efficient aerobic growth of P. putida. Specific growth rate was highest at pH 7. Feeding glucose and yeast extract continuously at the initial growth phase was the most effective way to get high cell density of P. putida.
Keywords
VOC; Pseudomonas putida; Fed-batch; Culture;
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