• Title/Summary/Keyword: inhibition of seed germination

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Identification and Growth Inhibition of Phytotoxic Substances from Tomato Plant (토마토식물의 독성물질 확인과 생장억제작용)

  • 김영식
    • Journal of Plant Biology
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    • v.32 no.1
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    • pp.41-49
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    • 1989
  • Phenolic compounds such as gallic acid, ferulic acid, p-hydroxybenzoic acid, vanillic acid, salicylic acid, tannic acid, and hydroquinone were identified from the aqueous extracts and volatile substances of tomato plant by paper chromatography, high performance liquid chromatography and gas chromatography. The seed germination and seedling growth of the experimental species, lettuce and egg plant, were severely inhibited in 5$\times$10-3M of phenolic reagents identical to those identified from tomato plant. Germination and growth rate of test species in 5$\times$10-4M and 5$\times$10-5M were higher than that of 5$\times$10-3M. Therefore, 5$\times$10-3M of phenolic compounds would be assumed to be threshold concentration for allelopathic effects.

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The Effect of Temperature of Compost on the Germination Inhibition of Weed Seeds during Livestock Manure Composting (가축 분 퇴비화과정의 온도에 따른 잡초종자 발아억제 효과)

  • Jeong, Kwang-Hwa;Kim, Ki-Yong;Lee, Dong-Jun;Lee, Dong-Hyun;Kwag, Jung-Hoon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.4
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    • pp.325-330
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    • 2018
  • This study was carried out to assess the composting temperature effects on germination of curled dock and barnyard millet seeds. After the seeds wrapped in gauze were buried in the compost heap, the seeds were taken out under monitering the temperature for measuring germination rate at 1.5, 3, 10 and 20 days of composting. The germination rate of the seeds was directly related to the temperature of the compost heap regardless of the type of composting method. The seeds that buried in the compost for 1.5 days at a temperature of $60{\sim}70^{\circ}C$ were completely lost their germination ability. However, the germination rate of the seeds retained about 10% even after 20 days when compost temperature was below $51^{\circ}C$. Reulsting data suggests that, it is necessary to keep the compost temperature at least $55^{\circ}C$ for more than 3 days in order to suppress the germination of the seeds. On the other hand, if the temperature of the compost rises by $60{\sim}70^{\circ}C$, the seeds will lose their gemination ability with in 1.5 days. In conclusion, aerobic composting would be more effective in suppressing germination ability of curled dock seed and barnyard millet seeds.

Allelopathic Inhibition by Extracts and Volatiles from Leaf and Seed of Sicklepod (Cassia tora L.) (결명자(決明子)(Cassia tora L.) 엽(葉)과 종자(種子)의 추출물(抽出物)과 휘발성분(揮發成分)의 타감성(他感性) 생육저해작용(生育沮害作用))

  • Lim, Sun-Uk;Kim, Geum-Sook;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.2
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    • pp.160-167
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    • 1992
  • The growth of weeds and some other plants has been considered to be inhibited by sicklepod(Cassia tora L.) sharing the habitat. The study was conducted, for the first time, to propose that this phenomenon is not only due to competition for physical and nutritional conditions but also due to allelopathy. In addition, autotoxicity of sicklepod was examined. The results are summarized as follows. 1. Rice sheath length reduced progressively from 18 % to 36 % with increasing the concentration of treating aqueous extracts of sickle pod seeds, but rice germination was not affected. In contrast, radish hypocotyl length was not reduced by the aqueous extract treatment but radish germination was significantly reduced by 66 % at 1 : 10 and 1 : 5 treatment. 2. Total chlorophyll contents in rice seedling decreased from 50 % to 65 % by treatment of seed aqueous extracts diluted from 1 : 50 to 1 : 5 ratio. 3. Aqueous extracts of sicklepod leaves significantly reduced hypocotyl length and fresh weight in radish and germination in rice, but mung bean was slightly affected by aqueous extracts only in fresh weight. 4. Volatiles from fresh, immature seeds with husk reduced the radish germination and seedling growth and radish root growth appeared to be more sensitive to the exposure to volatiles from fresh immature seeds than both germination and hypocotyl growth. 5. Volatiles from sickepod leaves inhibited germination and growth of radish, rice and mung bean, and seedling growth was more sensitive to volatiles from leaves than germination. 6. Volatiles from sicklepod leaves reduced germination and radicle length of sicklepod itself. 7. Collectively, it is concluded that there are water-soluble and volatile substances responsible for allelopathy in sicklepod.

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Ecological Effects of Zinc and Lead on Plants (식물체에 미치는 연, 아연 ( Pb, Zn ) 의 영향)

  • Park, Bong-Kyu;Kim, Ok-Kyung
    • The Korean Journal of Ecology
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    • v.6 no.2
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    • pp.98-105
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    • 1983
  • This study was carried out to investigate the effects of Zn and Pb concentration on seed germination and plant growth in water and soil culture, and the frequency of chlorosis invegetation and the relationship between plants and soil in the Sambo mine. The inhibition of germination were observed in 1000ppm of Zn, 10ppm of Pb and 5000ppm of Zn + Pb, but germination was more stimulated in 10ppm of Zn than control. The symptoms of chlorosis and abnormality were occurred in plant leaves grown to the soils treated with more than 1000ppm of Pb. Reasons of chlorosis were considered as an antagonistic effect of other metals towards uptake of iron by the plant in Zn treatment. The contents of Zn and Pb in fruits were lower than those of leaves, and that was remarked in case of Pb. With increasing rate of Zn and Pb treatment, chemical components of soils in pot culture were accompanied by slight decrease in pH, total nitorgen and exchangeable K. Chlorotic individuals of 10 species were shown in the areas of the Sambo mine. Chlorotic symptoms were especially extensive and severe in Sophora angustifolia, Populus alba, Spiraea prunifolia, Amorpha fruticota, Lespedeza bicolor and Salix dependens. Plants in the investigated areas grew in soils containing Zn of 311ppm and Pb of 151ppm on an average, and accumulated Zn of 2084ppm and Pb of 49ppm.

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Studies on Dormancy Breaking, Sowing Time and Inhibition of Germination during Storage of Seed in Wasabia japonica MATSUM (고추냉이 종자(種子)의 휴면타파(休眠打破), 저장중(貯藏中) 유아출현억제(幼芽出現抑制) 및 파종기(擺種期)에 관(關)한 연구(硏究))

  • Kim, Sun-Kon;Kim, Dong-Won;Whang, Chang-Ju;Nam, Sang-Sig
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.1
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    • pp.64-67
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    • 1996
  • This experiment was carried out to obtain the information on seed dormancy, germinability during storage in wasabia japonica Matsum. $GA_3$ 100ppm+BA 10ppm was more effective than $GA_3$100ppm alone in breaking the dormancy. Storage of seeds pretreated with growth retardants (CCC, Uniconazol) seemed to be a useful method for preventing germination during storage. The stratification was an effective storage method to germinate the seeds for sowing them in fall.

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Allelopathic Effects of Extracts of Trifolium repens on the Seed Germination and Seedling Growth of Zoysia japonica (토끼풀(Trifolium repens) 추출액이 잔디(Zoysia japonica)의 발아와 생장에 미치는 알레로파시 효과)

  • Lee, Ji-Hun;Kim, In-Taek;Lee, Ho-Jun;Kim, Yong-Ok
    • The Korean Journal of Ecology
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    • v.24 no.3
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    • pp.125-130
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    • 2001
  • The allelopathic effects of aqueous extracts from Trifolium repens were studied. Aqueous extracts of leaves, stems, roots and flowers of T. repens strongly inhibited the seed germination and seedling growth of Zoysia japonica. In general, the higher concentration of the extracts showed the more great inhibition effect. The phenolic compounds extracted from T repens were analysed and identified using gas chromatography. 14 phenolic compounds were isolated from the leaves, stems, roots and flowers: caffeic acid, p-hydroxy benzoic acid, ferulic acid, gallic acid, p-coumaric acid, vanillic acid, trans-cinnamic acid, 2,5 dihydroxy benzoic acid, syringic acid, 2-hydroxy cinnamic acid, benzoic acid, salicylic acid, phloroglucinol and phanylacetic acid. The seed germination and relative growth ratio of Z. japonica by 14 phenolic compounds were inhibited in whole treatment. Stronger growth inhibitor were benzoic acid, salicylic acid and trans-cinnamic acid. From these results it is suggested that the phenolic compounds from T. repens seemed to be responsible for the allelopathic potential.

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Herbicidal and Antifungal Activities of the aqueous extracts of Persicaria longiseta (개여뀌의 제초 및 항균활성 탐색)

  • Choi, Go-Bong;Woo, Seong-Bae;Song, Jin-Young;Kang, Jeong-Hwan;Kim, Tae-Keun;Kim, Hyoun-Chol;Song, Chang-Khil
    • Korean Journal of Organic Agriculture
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    • v.23 no.3
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    • pp.481-495
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    • 2015
  • The study researched germination of the plants and growth of Fungus according to concentration of aqueous extracts in order to provide basic data for developing natural agricultural resources by using Persicaria longiseta. The seed germination of Amaranthus spinosus was inhibited at 25% P. longiseta extract, while Agrostis stolonifera ssp. palustris was not affected at all concentrations tested. Especially, the seed germination rate and fresh weight of Trifolium incarnatum at 20% P. blumei extract were higher than those of control plot. The early growth of most receptor plant seedlings was promoted at 25% and 50% of P. blumei extracts, but the radicle growth of all receptor plants was significantly inhibited at > 25% of P. longiseta extract. The response of receptor plants to P. longiseta extract was different according to the plant species and the plant parts. The growth of plant pathogenic fungus in PDA medium showed an increasing inhibition tendency with increasing concentrations of P. longiseta extract. Especially, P. longiseta extract showed the greatest antimicrobial activity against Phytophthora infestans, Phythium graminicola, and Pythium venterpoolii. The content of total phenolic compound in P. longiseta was higher in leaves (1082.3 mg/L) but lower in roots (228.6 mg/L) and stems (207.8 mg/L), which is an allelopathic chemical. As these results are summarized, P. longiseta have competitive advantage because they release phenolic compounds with allelopathic effect and affect on germination, growth and fungi growth on underground flora compared to native plants and they have eligibility for natural herbicide and germicide.

Effect of Alfalfa Extract, It's Concentration and Absorbents on Germination and Growth of Alfalfa (알팔파 추출물, 추출농도 및 흡착제 처리가 알팔파 발아와 생육에 미치는 영향)

  • Ill Min, Chung;Ki-June Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.5
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    • pp.619-628
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    • 1995
  • Crude aqueous extracts from dried leaves, stems, roots, and flowers from both field grown and greenhouse grown alfalfa plants inhibited alfalfa seed germination and seedling growth. The degree of inhibition was greater in the field grown plant extracts. Flowers extract of field grown plant most inhibited alfalfa germination and seedling growth. In the concentration study, the highest concentration of extract (9.0%, w/v) significantly inhibited total alfalfa seed germination by 50% as compared to control. In partitioning study using pot hydroponic culture of plant biomass into leaves, stems, root, LAR products of LWR and SLA exhibited significant variation among four species. This result support that the inhibitory effect of autotoxic substances presenting in alfalfa tissue may be possible interference with the patitioning of biomass into leaf component relative to the total biomass produced by the alfalfa plant. Toxicity of extract was not reduced by adding activated charcoal, Dowex-50W, amberlite to the extract. Toxic substances existing in most plant tissues but mainly above ground foliage are water soluble and stable and may persist in old alfalfa fields. Thus, it is recommended to remove as much as possible of the above growth parts, especially vegetative stage, before one tries to re-establish alfalfa in former field of alfalfa.

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Varietal Difference of Viviparity and Germination - Inhibition of Rice Hull Extracts (벼 수발아의 품종간 차이와 왕겨 추출물질의 발아억제효과)

  • Kim, Bong Ku;Lee, Dong Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.434-440
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    • 1996
  • This experiment were conducted to elucidate the damage by viviparity during grain filling on grain quality and hulling recovery and to understand the varietal differences of viviparity and the effect of extractive from rice hull of hardly viviparous varieties on seed germination and seedling growth. Ten rice cultivars, such as three indica, four japonica and three Tongil-type rices, were used in this study. The brown rice yield was decreased along with increasing the artificial viviparous treatment periods. At six days after treatment, brown rice yield of Daeseongbyeo was reduce 20% as compared with control. The ratio of head rice was 41.3% at six days after viviparous treatment with $25^{\circ}C$ temperature and over 95% relative humidity, while it was 99.5% in control. Varietal differences of viviparity in three rice groups were ranged from 1.1 to 5.2% in indica rices, 9.2 to 79.7% in japonica rices and 2.1 to 63.7% in Tongil-type rices. Methanol extractives from rice hull of IR36, Shinunbongbyeo and Jungwonbyeo inhibited significantly the germination of Deaseongbyeo.

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Effects of Plant Growth Regulators and Sugars on the $\alpha-Amylase$ Activity in Cotyledons of Germinating Vigna angularis Seeds

  • Hong, Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.2 no.2
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    • pp.159-168
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    • 1998
  • Effect of plant growth regulators and end-product on the enzyme activities in cotyledons of Vigna angularis during germination was investigated by measuring the changes of $\alpha-amylase$ activities in attached and detached cotyledons applied growth regulators and sugars. The higher levels of $\alpha-amylase$ in detached cotyledons than those in cotyledons attached to the embryonic axis were due to both faster synthesis and slower degradation of the enzyme in the detached cotyledons than in the attached cotyledons. Levels of $\alpha-amylase$ activity were reduced by high concentrations of glucose and sucrose, and it is suggested that this effect was caused mostly by osmotic stress and partly by end-product repression. In detached cotyledons exogenously supplied $GA_3,$ IAA, kinetin, or their combinations has a small promotive effect on the developmental patterns of $\alpha-amylase$ activity ABA and uniconazole both prevented the synthesis of $\alpha-amylase$. Glucose inhibition of enzyme activity was partly reversed by the application of $GA_3,$ and CAMP. $GA_3,$ and cAMP seemed to act through a similar mechanism. The addition of inhibitors of protein and RNA synthesis largely prevented the increase of enzyme activity in the presence or absence of exogenous $GA_3,$. The pretreatment experiments with canavanine indicated that the earlier the time of addition was, the lower the amylase activity was.

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