• 한국축산식품학회지
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• 제35권4호
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• pp.466-472
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• 2015

A rapid and simple analytical method, using liquid chromatography tandem mass spectrometry (LC-MS/MS), was developed to detect myo-inositol (MI) in infant formulas. For protein removal: acid hydrolysis and lipid removal through organic solvent extraction. The operating conditions for instrumental analysis were determined based on previously reported analogous methods that used LC-MS/MS. Quantitative analysis was used for the detection limit test, infant formula recovery test, and standard reference material (SRM) 1849a to verify the validity of our LC-MS/MS analytical method, which was developed to quantify MI. For validation, the results of our method were compared with the results of quantitative analyses of certified values. The test results showed that the limit of detection was 0.05 mg/L, the limit of quantitation was 0.17 mg/L, and the method detection limit was 17 mg/kg. The recovery test exhibited a recovery between 98.07-98.43% and a relative standard deviation between 1.93-2.74%. Therefore, the result values were good. Additionally, SRM 1849a was measured to have an MI content of 401.84 mg/kg and recovery of 98.25%, which is comparable to the median certified value of 409 mg/kg. From the aforementioned results, we judged that the instrumental analysis conditions and preparation method used in this study were valid. The rapid analytical method developed herein could be implemented in many laboratories that seek to save time and labor.

• 한국식품위생안전성학회지
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• 제29권3호
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• pp.207-210
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• 2014

본 연구에서는 인터넷에서 판매되는 배달이유식의 위생안전성을 평가하고, B. cereus의 독소유전자 분포를 파악하여 즉석조리식품으로 구분되는 이유식의 B. cereus 기준설정을 위한 기초자료를 제공하고자 하였다. 기타 영 유아식 27건, 즉석조리식품 79건 등 총 106건에 대한 실험결과는 다음과 같다. 1. 이유식 106건의 세균수 검사결과 기타 영 유아식에서는 12건(44%)에서 최대 $2.5{\times}10^3CFU/g$, 즉석조리식품에서는 46건(58%)에서 최대 $1.1{\times}10^6CFU/g$으로 대체적으로 즉석조리식품에서 세균수가 많이 검출되었다. 2. 106건의 검체 중 총 9건에서 B. cereus가 $10{\sim}3.6{\times}10^3CFU/g$ 범위로 검출되었으며 모두 즉석조리식품이었다. 3. B. cereus의 독소유전자 중 cytK는 6주(66.6%), nheA 및 entFM은 8주(88.9%), bceT는 7주(77.8%), hblC는 3주(33.3%)에서 검출되었으며 구토독소인 cer은 2주(22.2%)에서만 증폭밴드가 나타났다. 4. 분리된 균주는 B. cereus와 B. thuringiensis를 구분하기 위한 시험법에서 모두 B. cereus로 나타났다.

• 한국축산식품학회지
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• 제31권6호
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• pp.944-951
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• 2011

본 연구에서는 조제분유 중의 콜레스테롤을 신속하고 정확하게 분석하기 위해 지방 추출 과정을 거치지 않는 직접 검화 방법을 선택하여 분석법 개발을 시도하였다. 조제분유 분말시료를 직접 검화 수기에 취하여 검화 온도, 검화 시간, KOH 농도의 3가지 인자에 대해 콜레스테롤 회수율이 가장 양호하게 나타나는 최적 검화조건을 확립하고, 검화 후 수세과정에서 액액 분배가 용이한 용매 조건도 확립하였다. 또한 콜레스테롤 피크의 완전한 분리를 위한 적정 기기 조건을 확립하였다. 그 결과, 시료 약 2 g에 16 M-KOH 10 mL를 넣고 $90^{\circ}C$에서 60분 가열하여 검화한 후 diethyl ether로 3회 추출하고 hexane을 최종시험용액으로 하여 기기분석을 했을 때의 회수율이 98.80%로서 가장 양호하게 나타났다. 본 연구를 통해 개발된 조제분유의 효율적인 액액분배 및 직접가열 검화법은 일원배치 분산법에 의해 유화가공식품의 콜레스테롤 분석법으로 유효성이 검증되었으며, 아울러 개발된 전처리 방법 및 기기 분석 조건을 활용해 다양한 분석 기관에서 신속 정확하고 효율적인 콜레스테롤 분석을 수행할 수 있을 것으로 기대된다. 또한 산업체의 품질관리 및 검증기관에서 필요시 모니터링에 적극 활용 가능할 것으로 사료되며, 이를 통해 유화가공식품 류의 함량표시 및 규격관리의 정확성과 효율성 증대에 기여하여 제조업체의 정확하고도 효과적인 품질 및 안전성 확보에 기여할 수 있을 것으로 판단된다. 특히 위해 물질에 대한 정확한 함량 판단이 중요한 조제분유 등의 영 유아용 식품의 안전성 확보에 지대한 공헌을 할 수 있을 것으로 기대된다.

• Journal of Community Nutrition
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• 제6권2호
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• pp.78-85
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• 2004

Children are the most nutrition sensitive sub-group of a population. The nutritional status of children should be especially emphasized at all levels. This study was performed to investigate the current status, trend, and influencing factors to malnutrition of infants and children in China. The study was mainly based on the China Health and Nutrition Survey which is a longitudinal study conducted in 8 provinces and the data on growth of children under 7 years of age in 9 cities in China. The result of this study showed that one fifth of the children under 5 years of age are still suffering from stunted growth and one tenth suffering from underweight. The nutrition intervention on children under 2 years of age, especially on those under 18 months should be emphasized. Better supplementary food can improve the nutritional status to decrease the prevalence of stunted children. Therefore, the development of supplementary food should be the priority and should be emphasized with breastfeeding.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.949-955
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• 2021

Previously, our research group isolated Bifidobacterium breve IDCC4401 from infant feces as a potential probiotic. For this study, we evaluated the safety of B. breve IDCC4401 using genomic and phenotypic analyses. Whole genome sequencing was performed to identify genomic characteristics and investigate the potential presence of genes encoding virulence, antibiotic resistance, and mobile genetic elements. Phenotypic analyses including antibiotic susceptibility, enzyme activity, production of biogenic amines (BAs), and proportion of D-/L-lactate were evaluated using E-test, API ZYM test, high-performance liquid chromatography (HPLC), and D-/L-lactic acid assay respectively. The genome of B. breve IDCC4401 consists of 2,426,499 bp with a GC content of 58.70% and 2,016 coding regions. Confirmation of the genome as B. breve was provided by its 98.93% similarity with B. breve DSM20213. Furthermore, B. breve IDCC4401 genes encoding virulence and antibiotic resistance were not identified. Although B. breve IDCC4401 showed antibiotic resistance against vancomycin, we confirmed that this was an intrinsic feature since the antibiotic resistance gene was not present. B. breve IDCC4401 showed leucine arylamidase, cystine arylamidase, α-galactosidase, β-galactosidase, and α-glucosidase activities, whereas it did not show production of harmful enzymes such as β-glucosidase and β-glucuronidase. In addition, B. breve IDCC4401 did not produce any tyramine, histamine, putrescine, cadaverine, or 2-phenethylamine, which are frequently detected BAs during fermentation. B. breve IDCC4401 produced 95.08% of L-lactate and 4.92% of D-lactate. Therefore, our findings demonstrate the safety of B. breve IDCC 4401 as a potential probiotic for use in the food industry.

• 한국식품과학회지
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• 제45권6호
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• pp.791-795
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• 2013

본 연구에서는 유제품 및 육제품에서 Staphylococcus aureus를 검출하기 위한 PCR과 배지배양법을 비교검증 하였다. 우유, 분유, 소시지, 소고기 분쇄육에 S. aureus를 인위적으로 접종시킨 후, 축산물 가공기준 및 성분규격에 따라 10% NaCl이 첨가된 tryptic soy broth(TSB)에서 증균배양 하였으며, Baird Parker agar에 선택배양 하였다. PCR은 TSB에서 1 mL를 채취한 후 DNA를 추출하여 시행하였으며, S. aureus의 23s rRNA를 표적으로 하는 primer를 사용하였다. 의심집락에 대해 coagulase test와 colony PCR을 통한 확인시험을 실시하였다. 실험결과 우유와 분유에서는 배지배양법과 PCR간에 통계학적 유의차가 존재하지 않았다. 소시지와 소고기 분쇄육의 경우에는 PCR이 배지법에 비해 더 많은 양성을 검출하여 높은 통계학적 유의차를 보였다(p<0.05). 연구결과를 종합하면, 유제품 및 육제품에서 PCR을 통한 S. aureus의 검출은 기존 배지배지배양법에 비해 시간과 노동력을 절감할 수 있는 효과적인 방법으로 나타났다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제23권6호
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• pp.511-520
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• 2020

Purpose: Thickened infant formulas reduce regurgitation frequency and volume. Because the digestive tolerance of locust bean gum-containing formulas is controversial, the effectiveness and tolerance of a locust bean gum-thickened formula in infants presenting with regurgitation was evaluated. No other interventions were allowed during the 1 month follow-up period. Methods: We conducted an open, prospective, observational study of a locust bean gum-thickened formula administered to infants presenting with moderate to severe regurgitation according to parents during 1 month. Effectiveness and tolerance were assessed by evaluating gastrointestinal symptoms and quality of life indicators. Results: A total of 2,604 infants with an average age of 9.3±4.3 weeks were included in this 1 month trial. Regurgitation frequency and estimated volume decreased significantly (p<0.001) and the episodes were resolved completely in 48% of the infants. A significant decrease in duration of crying and episodes of gas (p<0.001), with improvement in quality of life parameters, was observed. Stool frequency increased and stool consistency softened (p<0.001) to levels within the physiologic range, consistent with the increased fiber load (0.42 g/100 mL). Conclusion: Locust bean gum-thickened formula decreased infant regurgitation, was well tolerated, and improved parental quality of life. Stool composition and frequency of the infants remained within the physiologic range.

• 한국의류학회지
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• 제39권6호
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• pp.924-937
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• 2015

This study surveyed the recognition and credibility of infant's organic cotton apparel and examined the current quality of children's organic cotton clothes available in the market. This study contributes to improve fabric certification programs and safety standards by understanding customer opinions and actual quality. A customer survey revealed that customers believe that organic cotton products should not include chemicals such as pesticides, fluorescent whitening agents and chemical colors; in addition, customers have high expectations for safety as well as worries about the products' validity. Children's underwear was the most purchased item category among organic cotton products. Current organic cotton certification programs are voluntary with multiple organizations that provide various certification standards. A total of 34.2% of children's organic cotton clothes sold at online and offline stores were not certified, and 10.5% had invalid certification information. This may substantially lower the credibility of organic cotton products and requires immediate change. Examination of organic cotton products showed that products do not meet customer expectations for quality and safety: 3 out of 8 products used accessories (buttons and wappens) that include harmful chemicals such as lead, phthalate and formaldehyde, 6 products used fluorescent whitening agents, and 4 products used other fabrics such as rayon, polyurethane and polyester. GOTS mark is the most recognized organic certification, but it still allows the usage of fluorescent whitening agents, which indicates a gap between customer opinions and actual certification standards. Managing national-level mandatory certification programs like food and setting quality standards that meets customer expectations are required to enhance the credibility of organic cotton products.

• 한국축산식품학회지
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• 제35권6호
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• pp.765-771
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• 2015

The development of a sample preparation method and optimization of the analytical instrumentation conditions were performed for the determination of the vitamin B₁₂ content in emulsified baby foods sold on the Korea market. After removal of the milk protein and fats by chloroform extraction and centrifugation, the vitamin B₁₂ was water extracted from the sample. Following filtration of the solution through a nylon filter, the water-soluble extract was purified by solid-phase extraction using a Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The solution eluted from the cartridge was dried under a stream of nitrogen gas and reconstituted with 1 mL of water. The sample solution was injected into an LC-MS/MS system after optimizing the mobile phase for vitamin B₁₂ detection. The calibration curve showed good linearity with the coefficient of correlation (r²) value of 0.9999. The limit of detection was 0.03 µg/L and the limit of quantitation was 0.1 µg/L. The method of detection limit was 0.02 µg/kg. The vitamin B₁₂ recovery from a spiking test was 99.62% for infant formula and 99.46% for cereal-based baby food. The sample preparation method developed in this study would be appropriate for the rapid determination of the vitamin B₁₂ content in infant formula and baby foods with emulsified milk characteristics. The ability to obtain stable results more quickly and efficiently would also allow governments to exercise a more extensive quality control inspection and monitoring of products expected to contain vitamin B₁₂. This method could be implemented in laboratories that require time and labor saving.

• 한국식품위생안전성학회지
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• 제24권2호
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• pp.162-168
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• 2009

식품안전관련 사건 사고는 점차 대형화되면서 지속적으로 계속 발생하고 있다. 이에 본 연구에서는 1998년 1월부터 최근 2008년 10월까지 국내에서 발생한 주요 식품안전사건사고와 관련하여 다양한 언론매체에서 발표된 자료를 수집하고 그 발생현황에 대한 분석을 시도하였다. 총 1,206건이 발생한 것으로 조사되었으며, 이중 중복내용과 순수 식품안전사건 사고와 관련이 없는 부분을 제외하면 약 11년간 총 569건이 발생한 것으로 조사되었다. 이를 연도별로 보면 평균 51.7건이, 월별로 보면 평균 4.9건이 발생하였다. 주요 원인식품군에 있어 수산식품과 식육 및 육가공품, 과자류, 음료류, 특수영양식품군, 다류, 면류, 장류, 우유 및 유제품 등의 순으로 나타났으며, 식품유형에 있어서는 즉석섭취편의식품, 육류, 과자, 건강보조식품, 침출차, 영아용 조제식 및 육가공품, 인삼제품, 체중조절용 조제식품 등의 순으로 나타났다. 위해요소(원인물질)별로는 화학적 위해요소가 247건(43.4%)으로 가장 많이 발생하였으며, 다음이 생물학적 위해요소로 126건(22.1 %), 물리적 위해요소는 97건(17.0%)의 순으로 나타났다. 위해요소의 발생단계에 대한 분석을 보면, 원료차원에서 가장 많은 364건(64%)이 발생하였고, 다음이 제조가공단계로 151건(26.5%), 유통 및 판매단계가 44건(7.7%)으로 나타났다. 본 연구에서와 같은 자료의 정리 및 분석은 추후 식품안전정책 및 HACCP의무적용 등에 있어 우선적으로 다루어야 할 품목을 선정하는 데 있어 중요한 기초자료로 활용될 수 있을 것이다.