• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1095-1100
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• 2008

The role of exopolysaccharides (EPSs) from a plant growth-promoting rhizobacterium, Burkholderia gladioli IN26, on elicitation of induced systemic resistance was investigated. A purified EPS induced expression of PR-1a::GUS on tobacco and elicited induced resistance against Colletotrichum orbiculare on cucumber. The maximum level of disease protection was noted when seeds were soaked in 200 ppm of the EPS. Our results indicate that EPS from specific rhizobacteria can elicit induced resistance and suggest that bacterial EPS might be a useful elicitor of resistance under field conditions.

• The Plant Pathology Journal
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• 제21권1호
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• pp.7-12
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• 2005

Plant growth-promoting rhizobacteria (PGPR) are a wide range of root-colonizing bacteria with the capacity to enhance plant growth and control plant pathogens. Here we review recent progress that indicate some PGPR strains release a blend of volatile organic compounds (VOCs) that promote growth in Arabidopsis seedlings and induce resistance against Erwinia carotovora subsp. carotovora. In particular, the volatile components 2,3-butanediol and acetoin released exclusively from the PGPR strains triggered the greatest level of growth promotion and induced systemic resistance. Pharmacological applications of 2,3-butanediol promoted the plant growth and induced resistance, while bacterial mutants blocked in 2,3-butanediol and acetoin synthesis was devoid of growth-promotion and induced resistance capacities. The results suggested that the bacterial VOCs play a critical role in the plant growth promotion and induced resistance by PGPR. Using transgenic and mutant lines of Arabidopsis, we provide evidences that the signal pathway activated by volatiles from one PGPR strain is dependent on cyto-kinin activation for growth promotion and dependent on an ethylene-signaling pathway for induced pathogen resistance. This discovery provides new insight into the role of bacterial VOCs as initiators of both plant growth promotion and defense responses in plants.

• 약학회지
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• 제26권3호
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• pp.169-174
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• 1982

Several strains of bacteria having resistance to macrolide antibiotics were isolated. EMR-1, one of them, exhibited the induced resistance to macrolide antibiotics and this microorganism was identified as a bacterium belong to Bacillus species. The subinhibitory concentration of erythromycin or oleandomycin induced strong resistance to both erythromycin and oleandomycin themselves and to other macrolide antibiotics such as leucomycin, spiramycin and josamycin. The effective concentration of inducer, erythromycin was $0.0016-0.2\mu$g/ml. The inactivating enzyme of these antibiotics was not produced by EMR-1.

• 전기학회논문지P
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• 제59권4호
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• pp.434-439
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• 2010

This study fabricated a simulation facility which reduced the structure of a current distribution line to 50:1 in order to analyze the induced lightning shielding effect of a 22.9kV-Y distribution line according to ground resistance capacity, grounding locations, etc. When installing an overhead ground wire, the standard for grounding a distribution line with a current of 22.9kV-Y requires that ground resistance in common use with the neutral line be maintained less than $50\Omega$every 200m span. The reduced line for simulation had 7 electric poles and induced lightning was applied to the ground plane 2m apart from the line in a direction perpendicular to it using an impulse generator. If induced voltage occurred in the line and induced current flowed through the line due to the applied current, the induced voltage and current of the 'A' phase were measured respectively using an oscilloscope. When all 7 electric poles were grounded with a ground resistance of less than $50\Omega$ respectively, the combined resistance of the line was $7.4\Omega$. When an average current of 230A was applied, the average induced voltage and current measured were 1,052V and 13.8A, respectively. Under the same conditions, when the number of grounding locations was reduced, the combined resistance as well as induced voltage and current showed a tendency to increase. When all 7 electric poles were grounded with a ground resistance of less than $100\Omega$, the combined resistance of the line was $14.9\Omega$. When an average current of 236A was applied, the average induced voltage and current of the 'A' phase calculated were 1,068V and 15.6A, respectively. That is, in this case, only the combined resistance was greater than when all 7 electrical poles were grounded, and the induced voltage and current were reduced. Therefore, it is thought that even though ground resistance is slightly higher under a construction environment with the same conditions, it is advantageous to ground all electric poles to ensure system safety.

• Integrative Medicine Research
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• 제2권4호
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• pp.131-138
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• 2013

The skeletal muscle in our body is a major site for bioenergetics and metabolism during exercise. Carbohydrates and fats are the primary nutrients that provide the necessary energy required to maintain cellular activities during exercise. The metabolic responses to exercise in glucose and lipid regulation depend on the intensity and duration of exercise. Because of the increasing prevalence of obesity, recent studies have focused on the cellular and molecular mechanisms of obesity-induced insulin resistance in skeletal muscle. Accumulation of intramyocellular lipid may lead to insulin resistance in skeletal muscle. In addition, lipid intermediates (e.g., fatty acyl-coenzyme A, diacylglycerol, and ceramide) impair insulin signaling in skeletal muscle. Recently, emerging evidence linking obesity-induced insulin resistance to excessive lipid oxidation, mitochondrial overload, and mitochondrial oxidative stress have been provided with mitochondrial function. This review will provide a brief comprehensive summary on exercise and skeletal muscle metabolism, and discuss the potential mechanisms of obesity-induced insulin resistance in skeletal muscle.

• The Plant Pathology Journal
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• 제22권4호
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• pp.339-347
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• 2006

Riboflavin caused induction of systemic resistance in chickpea against Fusarium wilt and charcoal rot diseases. The dose effect of 0.01 to 20 mM riboflavin showed that 1.0 mM concentration was sufficient for maximum induction of resistance; higher concentration did not increase the effect. At this concentration, riboflavin neither caused cell death of the host plant nor directly affected the pathogen's growth. In time course observation, it was observed that riboflavin treated chickpea plants were inducing resistance 2 days after treatment and reached its maximum level from 5 to 7 days and then decreased. Riboflavin had no effect on salicylic acid(SA) levels in chickpea, however, riboflavin induced plants found accumulation of phenols and a greater activities of phenylalanine ammonia lyase(PAL) and pathogenesis related(PR) protein, peroxidase was observed in induced plant than the control. Riboflavin pre-treated plants challenged with the pathogens exhibited maximum activity of the peroxidases 4 days after treatment. Molecular weight of the purified peroxidase was 42 kDa. From these studies we demonstrated that riboflavin induced resistance is PR-protein mediated but is independent of salicylic acid.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2006년도 추계학술대회논문집
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• pp.208-211
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• 2006

Flow resistance induced by each element in suction nozzle of a vacuum cleaner with a rotary fan is investigated numerically and its relation with flow-induced noise is examined. Flow resistance and vorticity in suction nozzle are calculated and it is found that they are closely related with flow-induced noise. From numerical results, it is suggested that reduction of flow resistance effectively increases all-suction performance and decreases sound pressure level of noise generated in the suction nozzle.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.280-286
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• 2007

We evaluated a commercial biopreparation of plant growth-promoting rhizobacteria (PGPR) strains Bacillus subtilis GB03 and B. amyloliquefaciens IN937a formulated with the carrier chitosan (Bio Yield) for its capacity to elicit growth promotion and induced systemic resistance against infection by Cucumber Mosaic Virus (CMV) and Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana. The biopreparation promoted plant growth of Arabidopsis hormonal mutants, which included auxin, gibberellic acid, ethylene, jasmonate, salicylic acid, and brassinosteroid insensitive lines as well as each wild-type. The biopreparation protected plants against CMV based on disease severity in wild-type plants. However, virus titre was not lower in control plants and those treated with biopreparation, suggesting that the biopreparation induced tolerance rather than resistance against CMV. Interestingly, the biopreparation induced resistance against CMV in NahG plants, as evidenced by both reduced disease severity and virus titer. The biopreparation also elicited induced resistance against P. syringae pv. tomato in the wild-type but not in NahG transgenic plants, which degrade endogenous salicylic acid, indicating the involvement of salicylic acid signaling. Our results indicate that some PGPR strains can elicit plant growth promotion by mechanisms that are different from known hormonal signaling pathways. In addition, the mechanism for elicitation of induced resistance by PGPR may be pathogen-dependent. Collectively, the two-Bacilli strain mixture can be utilized as a biological inoculant for both protection of plant against bacterial and viral pathogens and enhancement of plant growth.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7913-7917
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• 2014

Apoptotic cell death plays a predominant role in histone deacetylase (HDAC) inhibitor-induced cytotoxicity. Nuclear morphological changes and activation of apoptotic executors are involved in CTS203-induced cell death. However, emerging issues of HDAC inhibitor-resistance have been observed in patients. Herein, MCF-7 cells were continuously exposed to CTS203 until the derived cells could proliferate normally in its presence. The newly obtained CTS203-resistant cells were nominated as MCF-7/203R. Compared to MCF-7 original cells, the MCF-7/203R cells were less sensitive to CTS203-induced apoptosis, with a minimal 6-fold higher $IC_{50}$ value. In contrast, the expression of Beclin-1 was dramatically up-regulated, positively correlated to the acquisition of CTS203-resistance. Our results revealed the participation of autophagy in acquired HDAC inhibitor-resistance and further identified Beclin-1 as a promising target for anti-drug resistance.

• 운동영양학회지
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• 제14권2호
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• pp.75-80
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• 2010

We evaluated the effect of the ginsenoside Re on insulin resistance of glucose transport in muscles of rats made insulin resistant with a high fat diet. After a week of adaptation period to the laboratory environment, 40 male wistar rats were randomly assigned into 2 groups (Chow diet group; CD, n = 20, High fat diet group; HFD, n = 20). After 5-week of high fat diet, Food was removed after 6:00 PM the day before the experiment. The following morning, rats were anesthetized by an intraperitoneal injection of pentobarbital sodium (50 mg/kg body wt), and the soleus muscles were removed. Before incubation, the soleus muscle was split longitudinally into strips with an average weight of 15~20 mg. After the muscle dissection was completed, the abdominal cavity was opened, and the epididymal, mesenteric, and retroperitoneal fat pads were removed and weighed. Treatment of muscles with ginsenoside Re alone had no effect on glucose transport. The high fat diet resulted in ~50% decreases glucose transport rate in soleus muscles. Treatment of muscles with ginsenoside Re in vitro for 90 min completely reversed the high fat diet-induced insulin resistance of glucose transport in soleus muscles. This effect of ginsenoside Re is specific for insulin stimulated glucose transport, as Re treatment did not reverse the high fat diet-induced resistance of skeletal muscle glucose transport to stimulation by contraction. Our results show that the ginsenoside Re induces a remarkably rapid reversal of high fat diet-induced insulin resistance of muscle glucose transport.