Browse > Article

A Two-Strain Mixture of Rhizobacteria Elicits Induction of Systemic Resistance Against Pseudomonas syringae and Cucumber Mosaic Virus Coupled to Promotion of Plant Growth on Arabidopsis thaliana  

Ryu Choong-Min (Laboratory of Microbial Genomics, Systems Microbiology Research Center, KRIBB)
Murphy John F. (Department of Entomology and Plant Pathology, Auburn University)
Reddy M.S. (Department of Entomology and Plant Pathology, Auburn University)
Kloepper Joseph W. (Department of Entomology and Plant Pathology, Auburn University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.2, 2007 , pp. 280-286 More about this Journal
Abstract
We evaluated a commercial biopreparation of plant growth-promoting rhizobacteria (PGPR) strains Bacillus subtilis GB03 and B. amyloliquefaciens IN937a formulated with the carrier chitosan (Bio Yield) for its capacity to elicit growth promotion and induced systemic resistance against infection by Cucumber Mosaic Virus (CMV) and Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana. The biopreparation promoted plant growth of Arabidopsis hormonal mutants, which included auxin, gibberellic acid, ethylene, jasmonate, salicylic acid, and brassinosteroid insensitive lines as well as each wild-type. The biopreparation protected plants against CMV based on disease severity in wild-type plants. However, virus titre was not lower in control plants and those treated with biopreparation, suggesting that the biopreparation induced tolerance rather than resistance against CMV. Interestingly, the biopreparation induced resistance against CMV in NahG plants, as evidenced by both reduced disease severity and virus titer. The biopreparation also elicited induced resistance against P. syringae pv. tomato in the wild-type but not in NahG transgenic plants, which degrade endogenous salicylic acid, indicating the involvement of salicylic acid signaling. Our results indicate that some PGPR strains can elicit plant growth promotion by mechanisms that are different from known hormonal signaling pathways. In addition, the mechanism for elicitation of induced resistance by PGPR may be pathogen-dependent. Collectively, the two-Bacilli strain mixture can be utilized as a biological inoculant for both protection of plant against bacterial and viral pathogens and enhancement of plant growth.
Keywords
Induced systemic resistance; plant growth promotion; Arabidopsis; PGPR;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 13  (Related Records In Web of Science)
연도 인용수 순위
1 Heil, M., A. Hilpert, W. Kaiser, and K. E. Linsenmair. 2000. Reduced growth and seed set following chemical induction of pathogen defence: Does systemic acquired resistance (SAR) incur allocation costs? J. Ecol. 88: 645-654   DOI   ScienceOn
2 Kokalis-Burelle, N., C. S. Vavrina, E. N. Rosskopf, and R. A. Shelby. 2002. Field evaluation of plant growth-promoting rhizobacteria amended transplant mixes and soil solarization for tomato and pepper production in Florida. Plant Soil 238: 257-266   DOI   ScienceOn
3 Kokalis-Burelle, N., M. S. Reddy, and J. W. Kloepper. 2006. Plant growth-promoting rhizobacteria as transplant amendments and their effects on indigenous rhizosphere microorganisms. Appl. Soil Ecol. 31: 91-100   DOI   ScienceOn
4 Murphy, J. F., M. S. Reddy, C.-M. Ryu, J. W. Kloepper, and R. Li. 2003. Rhizobacteria-mediated growth promotion of tomato leads to protection against Cucumber Mosaic Virus. Phytopathology 93: 1301-1307   DOI   ScienceOn
5 Ryu, C.-M., J. F. Murphy, K. S. Mysore, and J. W. Kloepper. 2004. Plant growth-promoting rhizobacteria protect systemically Arabidopsis thaliana against Cucumber Mosaic Virus by a salicylic acid and NPR1-independent and jasmonic acid-dependent signaling pathway. Plant J. 39: 381-392   DOI   ScienceOn
6 Zehnder, G. W., C. Yao, J. F. Murphy, E. R. Sikora, J. W. Kloepper, D. J. Schuster, and J. E. Polston. 1999. Microbeinduced resistance against pathogens and herbivores: Evidence of effectiveness in agriculture, pp. 335-355. In A. A. Agrawal, S. Tuzun, and A. Bent (eds.). Induced Plant Defenses Against Pathogens and Herbivores: Biochemisry, Ecology and Agriculture. APS Press, St. Paul, MN
7 Jetiyanon, K. W., D. Fowler, and J. W. Kloepper. 2003. Broad-spectrum protection against several pathogens by PGPR mixtures under field conditions. Plant Dis. 87: 1390- 1394   DOI   ScienceOn
8 Ryu, C.-M., C.-H. Hu, R. D. Locy, and J. W. Kloepper. 2005. Study of mechanisms for plant growth promotion elicited by rhizobacteria in Arabidopsis thaliana Plant Soil 286: 285- 292
9 Ryu, C.-M., M. Farag, C.-H. Hu, M. S. Reddy, H.-S. Wei, P. W. Pare, and J. W. Kloepper. 2003. Bacterial volatiles promote growth in Arabidopsis. Proc. Natl. Acad. Sci. USA 100: 4927-4932
10 Dietrich, R., K. Ploss, and M. Heil. 2005. Growth responses and fitness costs after induction of pathogen resistance depend on environmental conditions. Plant Cell Environ. 28: 211-222   DOI   ScienceOn
11 Zehnder, G. W., J. F. Murphy, E. J. Sikora, and J. W. Kloepper. 2001. Application of rhizobacteria for induced resistance. Eur. J. Plant Pathol. 107: 39-50   DOI   ScienceOn
12 Kloepper, J. W., C.-M. Ryu, and S. Zhang. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94: 1259-1266   DOI   ScienceOn
13 Vallad, G. E. and R. M. Goodman. 2004. Systemic acquired resistance and induced systemic resistance in conventional agriculture. Crop Sci. 44: 1920-1934   DOI   ScienceOn
14 Jetiyanon, K. and J. W. Kloepper. 2002. Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biol. Contr. 24: 285-291   DOI   ScienceOn
15 Garcia-Ruiz, H. and J. F. Murphy. 2001. Age-related resistance in bell pepper to Cucumber Mosaic Virus. Ann. Appl. Biol. 139: 307-317   DOI   ScienceOn
16 Lee, B. S., H.-B. Lee, S.-W. Choi, H.-S. Yun, and E.-K. Kim. 2005. Effective screening of antagonist for the biological control of soilborne infectious disease (damping-off). J. Microbiol. Biotechnol. 15: 701-709   과학기술학회마을
17 Heil, M. and I. Baldwin. 2002. Costs of induced resistance: Emerging experimental support for a slippery concept. Trends Plant Sci.7: 61-67   DOI   ScienceOn
18 Yan, Z., M. S. Reddy, C.-M. Ryu, J. A. McInroy, M. Wilson, and J. W. Kloepper. 2002. Induced systemic protection against tomato late blight elicited by plant growth-promoting rhizobacteria. Phytopathology 92: 1329-1333   DOI   ScienceOn
19 Tally, A., M. Oostendorp, K. Lawton, T. Staub, and B. Bassi. 1999. Commercial development of elicitors of induced resistance to pathogens, pp. 357-369. In A. A. Agrawal, S. Tuzun, and A. Bent (eds.). Induced Plant Defenses Against Pathogens and Herbivores: Biochemisry, Ecology and Agriculture. APS Press, St. Paul, MN
20 Wei, G., J. W. Kloepper, and S. Tuzun. 1996. Induced systemic resistance to cucumber diseases and rhizobacteria under field conditions. Phytopathology 86: 221-224   DOI
21 Menard, R., S. Alban, P. de Ruffray, F. Jamois, G. Franz, B. Fritig, J. C. Yvin, and S. Kauffmann. 2004. $\beta$-1,3 Glucan sulfate, but not $\beta$-1,3 glucan, induces the salicylic acid signaling pathway in tobacco and Arabidopsis. Plant Cell 16: 3020-3032   DOI   ScienceOn
22 Ryu, C.-M., J.-W. Kim, O.-H. Choi, S. Y. Park, S. H. Park, and C.-S. Park. 2005. Nature of a root-associated Paenibacillus polymyxa from field-grown winter barley in Korea. J. Microbiol. Biotechnol. 15: 984-991   과학기술학회마을
23 Murphy, J. F., G. W. Zehnder, D. J. Schuster, E. J. Sikora, J. E. Polston, and J. W. Kloepper. 2000. Plant growthpromoting rhizobacterial mediated protection in tomato against Tomato Mottle Virus. Plant Dis. 84: 779-784
24 Persello-Cartieaux, F., P. David, C. Sarrobert, M. C. Thibaud, W. Achouak, C. Robaglia, and L. Nussaume. 2001. Utilization of mutants to analyze the interaction between Arabidopsis thaliana and its naturally root-associated Pseudomonas. Planta 212: 190-198   DOI   ScienceOn
25 Ryu, C.-M., C. H. Hu, M. S. Reddy, and J. W. Kloepper. 2003a. Different signaling pathways of induced resistance by rhizobacteria in Arabidopsis thaliana against two pathovars of Pseudomonas syringae. New Phytol. 160: 413- 420   DOI   ScienceOn
26 Pare, P. W., M. A. Farag, C.-M. Ryu, and J. W. Kloepper. 2005. Elicitors and priming agents initiate plant defense responses. Photosynth. Res. 85: 149-159   DOI   ScienceOn
27 Kloepper, J. W., M. S. Reddy, D. S. Kenney, C. Vavrina, N. Kokalis-Burelle, and N. Martinez-Ochoa. 2004b. Theory and applications of rhizobacteria for transplant production and yield enhancement. Proc. XXVI IHC - Transplant Production and Stand Establishment. Eds. S. Nicola, J. Nowak, and C.S. Vavrina. Acta Horticul. 631: 217-219
28 Timmusk, S., B. Nicander, U. Granhall, and E. Tillberg. 1999. Cytokinin production by Paenibacillus polymyxa. Soil Biol. Biochem. 31: 1847-1852   DOI   ScienceOn
29 Kokalis-Burelle, N., C. S. Vavrina, M. S. Reddy, and J. W. Kloepper. 2003. Amendment of muskmelon transplant media with plant growth-promoting rhizobacteria: Effects on seedling quality, disease, and nematode resistance. Hortechnology 13: 476-482
30 Raupach, G. S., L. Liu, J. F. Murphy, S. Tuzun, and J. W. Kloepper. 1996. Induced systemic resistance in cucumber and tomato against cucumber mosaic cucumovirus using plant growth-promoting rhizobacteria (PGPR). Plant Dis. 80: 891-894   DOI   ScienceOn
31 Glick, B. R. 1995. The enhancement of plant growth by freeliving bacteria. Can. J. Microbiol. 41: 109-117   DOI   ScienceOn
32 Raupach, G. S. and J. W. Kloepper. 1998. Mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology 88: 1158- 1164   DOI   ScienceOn