• Title/Summary/Keyword: increase application

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Expression of Antioxidant Isoenzyme Genes in Rice under Salt Stress and Effects of Jasmonic Acid and ${\gamma}$-Radiation

  • Kim, Jin-Hong;Chung, Byung-Yeoup;Baek, Myung-Hwa;Wi, Seung-Gon;Yang, Dae-Hwa;Lee, Myung-Chul;Kim, Jae-Sung
    • Journal of Applied Biological Chemistry
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    • v.48 no.1
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    • pp.1-6
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    • 2005
  • Analysis of chlorophyll (Chl) fluorescence implicated treatment of 40 mM NaCl decreased maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm), actual quantum yield of PSII (${\Phi}_{PSII}$), and photochemical quenching (qP) in rice, but increased non-photochemical quenching (NPQ). Decreases in Fv/Fm, ${\Phi}_{PSII}$, and qP were significantly alleviated by $30\;{\mu}M$ jasmonic acid (JA), while NPQ increase was enhanced. Transcription levels of antioxidant isoenzyme genes were differentially modulated by NaCl treatment. Expression of cCuZn-SOD2 gene increased, while those of cAPXb, CATb, and CATc genes decreased. JA prevented salt-induced decrease of pCuZn-SOD gene expression, but caused greater decrease in mRNA levels of cAPXa and Chl_tAPX genes. Investigation of vacuolar $Na^+/H^+$ exchanger (NHX2) and 1-pyrroline-5-carboxylate synthetase (P5CS) gene expressions revealed transcription level of NHX2 gene was increased by JA, regardless of NaCl presence, while that of P5CS gene slightly increased only in co-presence of JA and NaCl. Unlike JA, ${\gamma}$-radiation rarely affected expressions of antioxidant isoenzyme, NHX2, and P5CS genes, except for increase in mRNA level of Chl_tAPX and decrease in that of pCuZn-SOD. These results demonstrate enhanced salt-tolerance in JA-treated rice seedlings may be partly due to high transcription levels of pCuZn-SOD, NHX2, and P5CS genes under salt stress.

An Exploratory Study on the Role of Empathy for Facilitating Smart Work (스마트워크 활성화를 위한 감정이입의 역할에 관한 탐색적 연구)

  • Kim, Yong-Young
    • Journal of the Korea Convergence Society
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    • v.8 no.5
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    • pp.201-211
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    • 2017
  • Social scientists have studied interaction between human beings, while computer scientists have expanded the research domain from human-human to human-machine, human-agent, or machine-machine. The reason why an adoption of Smart Work is failed is an anxiety about ICT usage which middle managers have. It is important to explore the concept both to reduce an anxiety on an application and to increase continuance to use it. Therefore this study takes "empathy" as a key factor to play a leading role both to relieve the anxiety about the application and to improve the intention to use it. The data is gathered from a survey of undergraduate who have experience to use MS-Access. The findings show that application empathy decrease the application anxiety, but the empathy increase the continuance mediated by cognitive and affective attitude.

Influence of Nitrogen Application and Shading on the Sterile-type Cold Injury in Rice (질소시비와 차광이 벼 장해형냉해에 미치는 영향)

  • Kim, Hee-Dong;Tetsuo, Satake;Kim, Young-Ho;Kim, Byeong-Hyeon;Ree, Dong-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.3
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    • pp.252-260
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    • 1989
  • This experiment was carried out to clarify the causes of sterility in terms of pollination characteristics for the sterile-type cold damage as influenced by amount of nitrogen application and shading conditions in rice plants. The results obtained are as follows: The number of young microspore per anther was not changed greatly by amount of nitrogen application. The number of ripened pollen grains per anther decreased according to increase in nitrogen application and shading degree, but the anther length and stigma length were not significantly affected by those factors. The number of pollen grains on stigma decreased by intense shading. The fertility decreased with increased nitrogen application and intensified shading. The elongation of auricle distance per day was less in less nitrogen application and intense shading. The internode length of the first and the second from the top were shortened with intense shading, but that of the fourth was elongated. The number of spikelet per panicle decreased with increase in shading intensity.

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Effect of Nitrogen Split Application Methods on Development of Vascular Bundle and Yield Components of Rice Cultivars

  • Lee, Dong-Jin;Chae, Je-Cheon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.4
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    • pp.237-240
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    • 2000
  • This experiment was conducted to evaluate the effect of split application of nitrogen(N) on development of vascular bundle(VB) and yield components of rice. Two cultivars were used in this study; IR58, an indica type and Shinunbongbyeim a japonica type. The number and total cross sectional area of the VB in the peduncle and leaf blade were more and bigger in N split application than 100 percent basal fertilizer. Nitrogen split application at necknode differentiation stage increased the number and size of the VB. Nitrogen split application resulted in increased panicle number with application of N before transplanting and at tillering stage; increased spikelets number with N application at necknode differentiation stage; and increased spikelet fertility and 1000 grain weight with N application at necknode differentiation and heading stages. Grain yield increased 7-10% in N split as compared to all basal application. The total cross sectional area of VB in peduncle closely correlated with the number of spikelets per panicle. Nitrogen management can have an impact on spikelet differentiation through more and bigger VB and increase grain yield potential.

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Effects of Chaenomelis fructus on the Muscle Atrophy Induced by Hindlimb Suspinsion in Rats (목과(木瓜)가 흰쥐의 후지체공(後肢滯空)에 의한 근강축(筋萎縮)에 미치는 영향(影響))

  • Kim, Na-Ri;Kim, Yeong-Gyun;Gwon, Jeong-Nam
    • The Journal of Internal Korean Medicine
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    • v.17 no.1
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    • pp.66-80
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    • 1996
  • This experimental Study was designed to investigate the effect of Chaenomelis Fructus on the muscle atrophy induced by hindlimb suspension in rats. The result are follows; 1. Chaenomelis Fructus significantl inhibited the increase of the activity of CK in serum. 2. Chaenomelis Fructus significantly inhibited the increase of the quantity of creatine in serum. 3. Chaenomelis Fructus significantl inhibited the increase of the activity of aldolase in serum. 4. Chaenomelis Fructus significantly inhibited the increase of the activity of LDH in serum. 5. Chaenomelis Fructus significantl inhibited the increase of the activity of AST in serum. 6. Chaenomelis Fructus inhibited the increase of the activity of ALT in serum. Based on the these results, it is concluded that the clinical application of Fructus chaenomelis can help cure muscle atrophy.

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Effects of Different Application Approaches with Diniconazole on the Inhibition of Stem Elongation and the Stimulation of Root Development of Cylindrical Paper Pot Seedling (생장조절체 처리가 원통형 종이포트묘의 도장 억제 및 근권부 발달에 미치는 영향)

  • Jang, Dong Cheol;Xu, Chan;Kim, Si Hong;Kim, Dae Hoon;Kim, Jae Kyung;Heo, Jae Yun;Vu, Ngoc Thang;Choi, Ki Young;Kim, Il Seop
    • Journal of Bio-Environment Control
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    • v.29 no.4
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    • pp.365-372
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    • 2020
  • This study was conducted to compare the effects of foliar spray and sub-irrigation of the triazole fungicide diniconazole on the regulation of stem elongation and to investigate the stimulation of root system development during the seedling stage. Comparing the two application approaches, there were significant differences in the leaf area, leaf area ratio (LAR), plant height, compactness, fresh shoot and root production, relative growth rate (RGR), and root to shoot ratio (R/S). At the same application concentration, the sub-irrigation showed a better retarding effect on growth than the foliar spray, because the PGR activity of diniconazole in root absorption was higher than that in shoot absorption. For reaching a target of 20% to 30% inhibition rate of stem length, foliar application concentration of diniconazole exceeded 10, however, only approximately 1 was required in the sub-irrigation application. The root system of tomato seedlings responded strongly to diniconazole application. Total root length, root volume, root average diameter, and the number of root tips increased when diniconazole was sub-irrigation application at 1. A reduction in fine roots (diameter range of 0 to 0.3 mm) and an increase in the roots with a diameter range of 0.3 to 0.6 mm was observed, and this may contribute to the increase in average diameter. The increase in root average diameter may be positive because root penetration increases with root diameter. Our results suggested that sub-irrigation maximized the PGR activity of diniconazole to enhance the retarding effect. And it also possible to enhance the tomato seedling root system by diniconazole stimulating with a lower concentration.

Disease Management in Road Trees and Pepper Plants by Foliar Application of Bacillus spp. (Bacillus spp. 엽면살포에 의한 가로수 및 고추의 병 방제)

  • Chung, Joon-hui;Ryu, Choong-Min
    • Research in Plant Disease
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    • v.22 no.2
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    • pp.81-93
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    • 2016
  • Out of plant-associated bacteria, certain plant growth-promoting bacteria (PGPB) have been reported to increase plant growth and productivity and to elicit induced resistance against plant pathogens. In this study, our objective was to broaden the range of applications of leaf-colonizing PGPB for foliar parts of road tress and pepper. Total 1,056 isolates of endospore-forming bacteria from tree phylloplanes were collected and evaluated for the enzymatic activities including protease, lipase, and chitinase and antifungal capacities against two fungal pathogens, Colletotrichum graminicola and Botrytis cinerea. Fourteen isolates classified as members of the bacilli group displayed the capacity to colonize pepper leaves after spraying inoculation. Three strains, 5B6, 8D4, and 8G12, and the mixtures were employed to evaluate growth promotion, yield increase and defence responses under field condition. Additionally, foliar application of bacterial preparation was applied to the road tress in Yuseong, Daejeon, South Korea, resulted in increase of chlorophyll contents and leaf thickness, compared with non-treated control. The foliar application of microbial preparation reduced brown shot-hole disease of Prunus serrulata L. and advanced leaf abscission in Ginkgo biloba L. Collectively, our results suggest that leaf-colonizing bacteria provide potential microbial agents to increase the performance of woody plants such as tree and pepper through spray application.

Buffering Contribution of Mitochondria to the $[Ca^{2+}]_i$ Increase by $Ca^{2+}$ Influx through Background Nonselective Cation Channels in Rabbit Aortic Endothelial Cells

  • Kim, Young-Chul;Lee, Sang-Jin;Kim, Ki-Whan
    • The Korean Journal of Physiology and Pharmacology
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    • v.9 no.1
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    • pp.29-35
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    • 2005
  • To prove the buffering contribution of mitochondria to the increase of intracellular $Ca^{2+}$ level ($[Ca^{2+}]_i$) via background nonselective cation channel (background NSCC), we examined whether inhibition of mitochondria by protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP) affects endothelial $Ca^{2+}$ entry and $Ca^{2+}$ buffering in freshly isolated rabbit aortic endothelial cells (RAECs). The ratio of fluorescence by fura-2 AM ($R_{340/380}$) was measured in RAECs. Biological state was checked by application of acetylcholine (ACh) and ACh ($10{\mu}M$) increased $R_{340/380}$ by $1.1{\pm}0.15$ ($mean{\pm}S.E.$, n=6). When the external $Na^+$ was totally replaced by $NMDG^+$, $R_{340/380}$ was increased by $1.19{\pm}0.17$ in a reversible manner (n=27). $NMDG^+$-induced $[Ca^{2+}]_i$ increase was followed by oscillatory decay after $[Ca^{2+}]_i$ reached the peak level. The increase of $[Ca^{2+}]_i$ by $NMDG^+$ was completely suppressed by replacement with $Cs^+$. When $1{\mu}M$ CCCP was applied to bath solution, the ratio of $[Ca^{2+}]_i$ was increased by $0.4{\pm}0.06$ (n=31). When $1{\mu}M$ CCCP was used for pretreatment before application of $NMDG^+$, oscillatory decay of $[Ca^{2+}]_i$ by $NMDG^+$ was significantly inhibited compared to the control (p<0.05). In addition, $NMDG^+-induced$ increase of $[Ca^{2+}]_i$ was highly enhanced by pretreatment with $2{\mu}M$ CCCP by $320{\pm}93.7$%, compared to the control ($mean{\pm}S.E.$, n=12). From these results, it is concluded that mitochondria might have buffering contribution to the $[Ca^{2+}]_i$ increase through regulation of the background NSCC in RAECs.

Effects of application rates of mineral N and cattle slurry on the dry matter yield and efficiency of N utilization of Reed canarygrass grown in different cutting frequency (예취빈도가 다른 조건에서 무기태 질소와 액상구비의 시용이 Reed canarygrass의 건물수량과 질소이용효율에 미치는 영향)

  • Lee, J.S.;Jo, I.H.
    • Journal of the Korea Organic Resources Recycling Association
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    • v.2 no.2
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    • pp.53-63
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    • 1994
  • In order to investigate the effects of application rates of mineral N and cattle slurry on the dry matter yield of Reed canarygrass grown in different cutting frequency, was was cernpared for the efficiency of N utilization. Annual rates of mineral N and cattle slurry N of 0 (control), 90kg, 180kg, 270kg and 360kg/ha in 3 cuttings, 0, 120kg, 240kg, 360kg and 480kg/ha in 4 cuttings, and 0, 150kg, 300kg, 450kg and 600kg/ha were applied as urea and cattle slurry in 5 cuttings, respectively. The results were summarized as follows: 1. The annual dry matter yields were increased with application rates of mineral N and cattle slurry. The annual dry matter yields of mineral N obtained were 14.4 ton/ha(ranged from 10.46 ton to 16.91 ton/ha) in 3 cuttings, 13.88 ton/ha(ranged from 9.91 ton to 16.53 ton/ha) in 4 cuttings and 15.98 ton/ha(ranged from 12.0 ton to 18.25 ton/ha) in 5 cuttings. The annual dry matter yields of cattle slurry obtained were 12.14ton/ha(ranged from 8.92 ton to 11.79ton/ha) in 3 cuttings, 10.81ton/ha(ranged from 8.92 to 11.79 ton/ha) in 4 cuttings and 12.98ton/ha(ranged from 10.68 ton to 14.85ton/ha) in 5 cuttings. 2. Relative dry matter yield of cattle slurry as compared to mineral N were 84.3%, 77.9% and 81.2% in 3, 4 and 5 cuttings. 3. Average increase in dry matter production(kgDM/kgN) tended to decrease with application rates of mineral N and cattle slurry, and higher cutting frequencies. Average increase in dry matter production obtained were higher values at rates of 30kg/ha/cut in both of mineral N and cattle slurry. Average increase in dry matter production to mineral N were 23.9kg, 18.8kg and 15.2kgin 3, 4 and 5 cuttings, respectively. 4. Average increase in total nitrogen yield(kgTN/kgN) to mineral N obtained were 0.46kg at rates of 60kg/ha/cut in 3 cuttings, and 0.45kg and 0.40kg at rates of 30kg/ha/cut in 4 and 5 cuttings. 5. Average increase in dry matter production(kgDM/kgN) to cattle slurry obtained were 13.7kg and 19.5kg at rates of 30kg/ha/cut in 3 and 4 cuttings, and 9.7kg at rates of 60kg/ha/cut in 5 cuttings. 6. Average increase in total nitrogen yield(kgTN/kgN) to cattle slurry was not concern to the rates of application. Average increase in total nitrogen yield to cattle slurry obtained were 0.11kg, 0.20kg and 0.21kg in 3, 4 and 5 cuttings. 7. Relative average increase in dry matter production of cattle slurry as compared to mineral N were 33.1%, 52.1% and 50.0% in 3, 4 and 5 cuttings. Relative average increase in total nitrogen yield of cattle slurry as compared to mineral N were 28.9%, 51.3% and 55.3% in 3, 4 and 5 cuttings.

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A Study On The Hospital Reservation System And Appointment System Using Smartphone (스마트폰을 이용한 병원 진료예약 및 진료시스템 연구)

  • Seo, Seong-Bum;Lee, Jae-Sang;Koo, Min-Jeong
    • The Journal of the Convergence on Culture Technology
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    • v.3 no.1
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    • pp.29-33
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    • 2017
  • As more than 80% of the entire Korean population uses smartphone, an increasing number of applications are being developed to increase the convenience in daily life. Users are also looking for applications associated with health, such as those that help them confirm their health status. While users are use most frequently a health care application, this study was conducted to review an application that can be used in real time to access hospital services. Namely, to manage the health status of users and patients, book online or offline appointments and go through the appointments. The study was conducted using the application of Baeksok University Hospital.