• 한국자원식물학회지
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• 제23권6호
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• pp.503-512
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• 2010

Anther derived double haploids (DHs) from sweet pepper genotypes ('Special', 'Derby', 'Bossanova', 'Fiesta', 'Debora' and 'Minipaprika') were used to study the agronomic variation in 2006. Ninety-nine successful DHs regenerants (32 from 'Special', 25 from 'Derby', 23 from 'Bossanova', 10 from 'Fiesta', 6 from 'Debora' and 3 from 'Minipaprika') were transplanted at plastic house and studied on their agronomic characters. Variation in agronomic characters was observed within the DHs of each genotype. DHs obtained from 'Derby' and 'Fiesta' exhibited wide variation in fruit yield $plant^{-1}$ whereas averaged fruit yield $plant^{-1}$ was highest in 'Derby' (1608 g) and less variation was observed in DHs of 'Bossanova'. Based on the agronomic characters expressed in DHs population at this environment, SP55, SP56, SP60, and SP116 from 'Special', SP8, SP10, SP14, SP16, and SP34 from 'Derby', SP115, SP119, SP142, SP143, SP196, and SP199 from 'Bossanova', SP41, SP45, and SP114 from 'Fiesta', SP21 from 'Debora' and SP91 from 'Minipaprika' identified as elite inbred lines and these DH lines could be used for commercial hybrids production in sweet pepper. Genetic relationship among the selected inbred lines using molecular markers and their response to diseases are further recommended to study.

• Plant Resources
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• 제1권1호
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• pp.26-32
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• 1998

This paper describes the variations in eight agronomic traits in three unadapted local landraces and an inbred cultivar of corn. To compare the agronomic traits in field evaluation with molecular marker evaluation the genotypes of the plant introduction were also checked by 4 microsatellite-SSR loci. The variations of the eight agronomic traits were higher in the local landrades than in the inbred line. which was substantiated by the high genetic variation in the landrades with microsatellite-SSR loci. The level of genetic variation was also different between landraces. Since the genetic evaluation can be easily quantified by the analysis of microsatellite-SSR loci. the threshold level of genetic homogeneity in the population for parental lines in breeding program can be determined and the effort of maintaining the landrace population would be alleviated. As an example in our analysis. the entry from Whachon should not need the same number of selfing generations as the other two landraces to get the level of inbred state. Since this line showed lowest intra-genetic variation within the population.

• International Journal of Industrial Entomology and Biomaterials
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• 제20권2호
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• pp.79-85
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• 2010

Eight bivoltine inbred lines of silkworm Bombyx mori L. viz., AP8, AP10, AP17 and AP18 with marked female and plain male larvae and AP1, AP54, AP71 and AP72 with all plain larvae synthesized from Chinese commercial hybrids were evaluated for their general combining ability (gca), specific combining ability (sca) and reciprocal effects for eight quantitative traits. The line AP18 was found to be best general combiner by virtue of significant positive and positive gca effects for seven out of eight traits studied followed by the lines AP10, AP8 and AP17. Seven hybrids recorded significant positive and positive sca effects for all the traits under study reflecting the interaction of alleles, additive and non additive gene action and dominant effects expressed by genes. Among the 28 reciprocal hybrids, AP54$\times$AP18 and AP1$\times$AP17 revealed significant reciprocal effects for the six traits followed by AP54$\times$AP8 for five and AP10$\times$AP71 for four traits.

• Genomics & Informatics
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• 제18권3호
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• pp.29.1-29.11
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• 2020

Maize seed pigmentation is one of the important issue to develop maize seed breeding. The differently gene expression was characterized and compared for three inbred lines, such as the pigment accumulated seed (CM22) and non-pigmented seed (CM5 and CM19) at 10 days after pollination. We obtained a total of 63,870, 82,496, and 54,555 contigs by de novo assembly to identify gene expression in the CM22, CM5, and CM19, respectably. In differentially expressed gene analysis, it was revealed that 7,044 genes were differentially expressed by at least two-fold, with 4,067 upregulated in colored maize inbred lines and 2,977 upregulated in colorless maize inbred lines. Of them,18 genes were included to the anthocyanin biosynthesis pathways, while 15 genes were upregulated in both CM22/5 and CM22/19. Additionally, 37 genes were detected in the metabolic pathway concern to the seed pigmentation by BINs analysis using MAPMAN software. Finally, these differently expressed genes may aid in the research on seed pigmentation in maize breeding programs.

• 원예과학기술지
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• 제33권3호
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• pp.443-447
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• 2015

'원교3115호'와 '원교3116호'는 종자와 영양체로 번식이 가능한 $S_9$세대의 근교계 품종으로 잡종강세 현상을 이용한 $F_1$ 품종육성을 위한 중간모본용으로 육성되었다. '원교 3115호'는 'Benihoppe' 유래 자식 계통($S_9$ 세대)으로 초형은 중간 직립형이고 초세는 원 품종보다 약하다. 화아분화는 빠른 편이고 화방당 꽃 수는 10개 내외이다. 과형은 원추형, 과색은 연분홍색이며 평균과중은 11g 내외의 소과종으로 수량은 원품종과 비교하여 매우 낮은 편이다. 이 품종의 가장 큰 특징으로 경도가 대조 품종에 비해 2배 정도 강하다. '원교 3116호'는 'Doyonoka' 유래 자식 계통으로 초형은 직립형이고 초세는 원 품종보다 약하다. 화아분화는 빠른 편이고 화방당 꽃 수는 8개 내외이다. 과형은 편구형, 과색은 적색이며 평균 과중은 12g 내외로 소과종이다. '원교 3116호'는 다른 근교 계통들에 비해 수량성이 높고 과형이 우수하다는 것이 가장 큰 특징이다. 품질에 있어서도 두 품종 모두 당산비가 적절하여 식미가 좋으나, 탄저병과 흰가루병에는 이병성이고 진딧물과 응애 등 해충에 대해서도 다소 약한 경향이다.

• 한국양봉학회지
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• 제32권3호
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• pp.147-154
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• 2017

꿀벌은 화분매개 뿐만 아니라, 양봉산물을 생산하는 주요한 산업곤충 중 하나이다. 최근 농촌진흥청과 예천곤충 연구소에서는 국내 최초로 수밀력 우수 꿀벌 품종인 '장원벌'을 선발하여 보급하고 있다. 본 연구에서는 장원벌 계통 특이 분자 마커 개발을 위해 장원벌 부계인 D계통 특이적인 분자 마커를 개발 하고자 Sequence-Based Genotyping (SBG) 분석을 수행하였다. SGB 분석은 농촌진흥청 국립농업과학원에서 보존 육성중인 A, C, D, E, F 5개 기본종 계통에 대해 수행되었으며, 이를 통해 1,029개 SNP를 확보 할 수 있었다. 이후 A, C, D, F, E 기본종 계통 및 $D{\times}F$ 교배계통에 대한 SNP filtering 및 validation을 통해 최종적으로 AmD6 및 AmD9 두 개의 SNP 마커를 선발 하였으며, genotyping 분석을 통해 AmD9 마커가 장원벌 부계인 D 계통을 100% 구분 할 수 있는 것으로 확인되었다. 본 마커를 통해 D 계통 및 장원벌을 보다 정확하게 판별하고 육종에 활용 할 수 있을 것으로 기대하고 있다.

• 농업과학연구
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• 제33권2호
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• pp.149-157
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• 2006

자식 계통간의 초기생육을 비교 시험한 결과를 요약하면 다음과 같다. 1. "단양찰" 계통은 파종후 출현이 다른 계통보다 2~3일정도 빨랐으며 출아후 2일째에는 다른 계통 보다 생육이 느렸지만 출아후 4일째에 서서히 빨라져서 출아후 8일째에는 15.6cm로 초기에 컸던 "당진/칠보" 자식계통보다 오히려 컸다. 2. 지상부의 생체중은 출아후 2일째에 9계통 모두 차이가 없었으나 출아후 8일째에는 서로간의 차이가 커서 "단양찰" 계통이 0.473g으로 다른 계통들 보다 오히려 무거웠다. 3. 지상부의 건물중 역시 출아후 8일째에 공시계통 중에서 "단양찰" 계통이 0.045g으로 무거웠으며 New Dangjin 계통이 0.018g으로 가장 가벼웠다. 4. 근장은 "단양찰" 계통이 출아후 2일에 12.22cm로 가장 길었고 출아후 4일째에는 28.6cm, 출아후 8일째에는 64.4cm로 다른 계통 보다 길었다. 5. 뿌리의 생체중 역시 "단양찰" 계통이 출아후 2일, 4일, 8일에 각각 0.091g, 0.238g, 0.471g 등으로 다른 계통들 보다 무거웠다. 6. 배유의 생체중은 "단양찰" 계통이 출아후 2일에 0.350g으로 무거웠고 출아후 8일째에는 0.261g으로 평균 0.222g 보다 무거운 편이었다. 그러나 다른 계통에 비하여 출아후 8일에는 배유무게의 감소가 뚜렷하였다. 또한 배유의 건물중도 출아후 2일째에 0.204g, 출아후 4일째에는 0.150g으로 가장 무거웠지만 오히려 출아후 8일째에는 0.084g으로 가벼워 출아후 4일째부터 지상부로 양분의 이동이 빨리 진행 되어 배유의 양분이 급속히 감소되는 현상을 보였다. 7. 초장과 근장은 $0.56^{**}$로 고도의 정의 상관을 보였고 지상부의 생체중은 지상부의 건물중, 뿌리의 생체중 배유의 생체중에서 고도의 정의 상관을 보였으나 배유의 건물중과 뿌리의 건물중과는 $-0.29^{**}$로 고도의 부의 상관을 보였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.153-157
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• 2005

Recently two breeding programs were carried out for isolation of new parental inbred lines in Iran. This study was undertaken in order to estimate the combining ability effects and heterosis of these lines. For this purpose an $8\times8$ diallel cross analysis including eight inbred lines of silkworm with four lines from each program were studied for their five quantitative traits. The results indicated that reciprocal effects appeared insignificant for most of traits. Japanese lines of 111 and 113 were best combiners for shell weight, cocoon weight and shell percentage traits. With respect to pupation rate, Chinese line 110 was the best and according to general combining ability (GCA) and specific combining ability (SCA) variances, it is superior in specific combinations. Hybrid $109\times110$ showed better characters for both productive and viability traits. Hybrids with high heterosis had high SCA too.

• 한국작물학회지
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• 제44권1호
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• pp.70-73
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• 1999

This study was conducted to ascertain the chromosomal locations and effect of quantitative trait loci (QTL) associated with the physical traits of rice (Oryza sativa L.) eating quality. One hundred sixty four recombinant inbred lines (MGRILs) of F$_{11}$ were derived from the cross between Milyang 23 (Tongil type) and Gihobyeo (japonica type). They were evaluated for six physical traits of cooked rice. Transgressive segregation was observed for all examined traits. Significant QTL were detected (LOD$\geq$2.0) in three traits, including single QTL for adhesiveness, gumminess, and chewiness of cooked rice, respectively. Phenotypic variation explained by each QTL ranged from 6.3% to 14.6%. However, no significant QTL was detected for hardness, cohesiveness, and elasticity of cooked rice. Pleiotropic effects of single QTL on different traits are observed.d.

• Journal of Plant Biology
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• 제5권3호
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• pp.21-24
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• 1962

Intergeneric crossings between the inbred line of Brassica pekinensis var. Chongbangkokun and inbred line of Raphanus sativus var. Oolsanjaelae were made using Brassica as female plant, and obtained two individuals of intergeneric hybrids. Morphological characters of the two F1 hybrids are mostly intermediate of the two parental species, and their somatic chromosome number is 19. Meiotic behaviors of the parental plants were normal, while those of F1 plants were extremely irregular. The mean pairing frequencies per cell of hybrids are 1.09II＋16.811(examined in May), and 1.76II＋15.47i(examined in June). Majority of the pollen grain of parent species are normal, whereas those of F1's are invariably abortive. No seed setting was obtained in the hybrid plants left in the open field.