Genomics & Informatics

Korea Genome Organization (한국유전체학회)
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The comparative gene expression concern to the seed pigmentation in maize (Zea mays L.)

  • Sa, Kyu Jin (Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Choi, Ik-Young (Department of Agriculture and Life Industry, Kangwon National University) ;
  • Lee, Ju Kyong (Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University)
  • Received : 2020.05.06
  • Accepted : 2020.07.20
  • Published : 2020.09.30
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Abstract

Maize seed pigmentation is one of the important issue to develop maize seed breeding. The differently gene expression was characterized and compared for three inbred lines, such as the pigment accumulated seed (CM22) and non-pigmented seed (CM5 and CM19) at 10 days after pollination. We obtained a total of 63,870, 82,496, and 54,555 contigs by de novo assembly to identify gene expression in the CM22, CM5, and CM19, respectably. In differentially expressed gene analysis, it was revealed that 7,044 genes were differentially expressed by at least two-fold, with 4,067 upregulated in colored maize inbred lines and 2,977 upregulated in colorless maize inbred lines. Of them,18 genes were included to the anthocyanin biosynthesis pathways, while 15 genes were upregulated in both CM22/5 and CM22/19. Additionally, 37 genes were detected in the metabolic pathway concern to the seed pigmentation by BINs analysis using MAPMAN software. Finally, these differently expressed genes may aid in the research on seed pigmentation in maize breeding programs.

Keywords

Acknowledgement

This study was supported by the Cooperative Research Program for Agriculture Science & Technology Development (project no. PJ013157012020 and PJ0131572020), Rural Development Administration (RDA), and the Golden Seed Project (No. 213009-05-1-WT821, PJ012650012017), Ministry of Agriculture, Food, and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Korea Forest Service (KFS), Republic of Korea.

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