• 소성∙가공
• /
• 제33권2호
• /
• pp.118-122
• /
• 2024

Micro-electromechanical systems (MEMS) based in-situ heating holders have been developed to enable high resolution imaging of heat treatment analysis. However, unlike the standard 3 mm metal disk specimens used in the furnace-based heating holder and general transmission electron microscopy holder, the MEMS-based in-situ heating holder requires thin specimens that can be penetrated by electrons to be transferred onto the MEMS chip. Previously, focused ion beam milling was used to transfer metal specimens, but it has the disadvantage of being expensive and the risk of specimen damage due to gallium ions. Therefore, in this study, we devised a method of transferring metallic materials by ultrasonic treatment using a transmission electron microscopy specimen made by electro jet polishing. A 3mm electropolished metal disk was placed in an appropriate solution, ultrasonicated, and then drop casted. The transfer of the specimen was successful, but it was confirmed that dislocations were formed inside the specimen due to ultrasonic treatment. This study provides a novel method for transferring metallic materials onto MEMS chips, which is cost-effective and less gallium ion damaging to the specimen. The results of this study can be used to improve the efficiency of heat treatment analysis using MEMS-based in-situ heating holders.

• 한국어병학회지
• /
• 제26권3호
• /
• pp.163-171
• /
• 2013

PCR (polymerase chain reaction) 법은 신속하고 정확하여 바이러스성 질병진단을 위해 널리 사용되지만 조직병리학적인 정보를 제공하지 못한다. 반면에 in-situ hybridization (ISH) 법을 사용하면 바이러스를 빠르게 검출할 수 있을 뿐 아니라 조직에서의 분포도 알 수 있다. 본 연구에서는 RSIV, VHSV, 그리고 VNN 바이러스들의 조직내 분포 및 조직 병리학적인 특성을 확인하기 위하여 이 바이러스들에 감염된 에 감염된 양식 넙치의 어류의 다양한 조직들을 대상으로 ISH 법을 적용하였다. 그 결과 이들 세 종류의 바이러스가 각각 다른 조직 및 세포들에 감염함을 확인 할 수 있었다. 본 연구 결과는 ISH법이 어류 병원성 바이러스의 신속 검출 뿐 아니라 조직 병리학적인 특성 확인에도 유용함을 제시한다.

• Transactions on Electrical and Electronic Materials
• /
• 제7권3호
• /
• pp.145-148
• /
• 2006

In this study, the response surface modeling of the pretilt angle control using in-situ photoalignment method with oblique UV exposure .on plastic substrate is investigated. The pretilt angle is the main factor to determine the alignment of the liquid crystal display. The response surface model is used to analyze the variation of the pretilt angle on the various process conditions. Heating temperature and UV exposure time are considered as input factors. The liquid crystal (LC) pretilt angle increased with increasing heating temperature and UV exposure time. The analysis of variance is used to analyze the statistical significance and the effect plots are also investigated to examine the relationship between the process parameters and the response.

• 한국지반신소재학회논문집
• /
• 제10권1호
• /
• pp.1-8
• /
• 2011

연약지반상에 구조물을 축조하게 되면 지반의 지지력 부족, 과대침하량 및 측방변형과 같은 문제점이 발생한다. 팽이말뚝기초공법은 지지력이 다소 부족한 지반에 팽이형 콘크리트 파일을 연약지반기초에 사용하여 지지력 증가와 침하억제, 부등침하방지 등의 효과를 도모하는 연약지반 표면 처리용 강성매트공법이며, 공사비 절감 등의 경제적인 효과와 공사기간 단축, 공사 중 소음 등으로 인한 민원 차단 등의 시공에 따른 편의성을 확보할 수 있다. 따라서 본 연구에서는 현장평판재하시험을 통하여 연약지반상의 현장타설형 팽이말뚝기초의 지지력을 산출하였다. 평판재하시험 분석결과를 통해 기존 연구 및 설계에서 적용하고 있는 제안식과 비교분석하여 현장타설형 팽이말뚝기초를 기초지반에 적용할 시 기초의 합리적인 범위를 산정하여 향후의 설계에 그 결과를 활용하고자 한다.

• 한국농공학회:학술대회논문집
• /
• 한국농공학회 2000년도 학술발표회 발표논문집
• /
• pp.431-437
• /
• 2000

The aim of the work described in this paper is to develope a pavement method for farm road. To this ends, a series of material test are conducted on in-situ soil which was mixed additives-coarse aggregate, polypropylene fiber, excellent soil compound etc. With the laboratory test results, in-situ pavement test was conducted during two years. The serious problem of the pavement is not appeared up to this time. And the measurement of field data is continued presently. The majority merits of this pavement method is low cost and using environmental materials.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권6호
• /
• pp.2823-2828
• /
• 2012

Objective: To explore a new model of in-situ xenograft lymphangiogenesis of human colonic adenocarcinomas in nude mice. Method: On the basis of establishing subcutaneous xenograft lymphangiogenesis model of human colonic adenocarcinoms, in-situ xenografts were established through the in situ growth of the HT-29 human colonic adenocarcinoma cell line in nude mice. The numbers of lymphangiogenic microvessels, the expression of lymphatic endothelial cell markers lymphatic vessel endothelial hyaloronic acid receptor-1 (LYVE-1), D2-40 and the lymphatic endothelial growth factors vascular endothelial growth factor-C (VEGF-C), -D (VEGF-D) and receptor-3 (VEGFR-3) were compared by immunohistochemical staining, Western bolt and quantitative RT-PCR in xenograft in-situ models. Results: Some microlymphatics with thin walls, large and irregular or collapsed cavities and increased LMVD, with strong positive of LYVE-1, D2-40 in immunohistochemistry, were observed, identical with the morphological characteristics of lymphatic vessels and capillaries. Expression of LYVE-1 and D2-40 proteins and mRNAs were significantly higher in xenograpfts in-situ than in the negative control group(both P<0.01). Moreover, the expression of VEGF-C, VEGF-D and VEGFR-3 proteins and mRNAs were significantly higher in xenografts in-situ (both P<0.01), in conformity with the signal regulation of the VEGF-C,-D/VEGFR-3 axis of tumor lymphangiogenesis. Conclusions: In-situ xenografts of a human colonic adenocarcinoma cell line demonstrate tumor lymphangiogenesis. This novel in-situ animal model should be useful for further studying mechanisms of lymph node metastasis, drug intervention and anti-metastasis therapy in colorectal cancer.

• International Journal of Ocean System Engineering
• /
• 제1권2호
• /
• pp.60-67
• /
• 2011

This paper presents an in-situ correction method to compensate for the position error of an autonomous underwater vehicle (AUV) near the sea floor. AUVs generally have an inertial navigation system assisted with auxiliary navigational sensors. Since the inertial navigation system shows drift in position without the bottom reflection of a Doppler velocity log, external acoustic positioning systems, such as an ultra short baseline (USBL), are needed to set the position without surfacing the AUV. The main concept of the correction method is as follows: when the AUV arrives near the sea floor, the vehicle moves around horizontally in a circular mode, while the USBL transceiver installed on a surface vessel measures the AUV's position. After acquiring one data set, a least-square curve fitting method is adopted to find the center of the AUV's circular motion, which is transferred to the AUV via an acoustic telemetry modem (ATM). The proposed method is robust for the outlier of USBL, and it is independent of the time delay for the data transfer of the USBL position with the ATM. The proposed method also reduces the intrinsic position error of the USBL, and is applicable to the in-situ calibration as well as the initialization of the AUVs' position. Monte Carlo simulation was conducted to verify the effectiveness of the method.

• Fisheries and Aquatic Sciences
• /
• 제11권2호
• /
• pp.103-112
• /
• 2008

Compressional sound velocities of shelf sediments in the South Sea of Korea, were measured in situ and in the laboratory for six cores. In situ sound velocity was measured using the Acoustic Lance (frequency of 7.5-15 kHz), while laboratory velocity was measured by the pulse transmission technique (frequency of 1MHz). Physical properties were relatively uniform with sediment depth, suggesting little effect of sediment compaction and/or consolidation. Average in situ velocity at each core site ranged from 1,457 to 1,488 m/s, which was less than the laboratory velocity of 1,503 and 1,604m/s. In muddy sediments the laboratory velocity was 39-47 m/s higher than in situ velocity. In sandy sediments, the difference was greater by an average of 116 m/s. Although the velocity data were corrected by the velocity ratio method based on bottom water temperature, the laboratory velocity was still higher than the in situ velocity (11-21 m/s in muddy sediments and 91 m/s in sandy sediments). This discrepancy may be caused by sediment disturbance during core collection and/or by the pressure of Acoustic Lance insertion, but it was most likely due to the frequency difference between in situ and laboratory measurement systems. Thus, when correcting laboratory velocity to in situ velocity, it is important to consider both temperature and frequency.

• 산업기술연구
• /
• 제28권B호
• /
• pp.111-116
• /
• 2008

Since the hydraulic fracturing field testing method was introduced first to Korean geotechnical engineers in 1994, there have been lots of progresses in a hardware system as well as an interpretation tool. The hydrofracturing system of first generation was the pipe-line type, and it has been developed to a wire-line system at their second generation. The current up-to-date system is more compact and is able to be operated by all-in-one system. With a progress in a hardware system, the software for analyzing in-situ stress regime has also been progressed. The shut-in pressure, which is the most ambiguous parameter to be obtained from hydrofracturing pressure curves, can now be acquired automatically from the various methods. While the hardware and software for hydrofracturing tests are being developed during the last decade, the author could accumulate the field test results which can cover the almost whole area of South Korea. Currently these field data are used widely in a feasibility study or a preliminary design step for tunnel construction in Korea. Regarding the difficulties in a site selection and a test performance for the in-situ stress measurement at an off-shore area, the in-situ stress regime obtained from the field experiences in the land area can be used indirectly for the design of a sub-sea tunnel. From the hydrofracturing stress measurements, the trend of magnitude and direction of in-situ stress field was shown identically with the geological information in Korea.

• 한국재료학회지
• /
• 제21권3호
• /
• pp.174-179
• /
• 2011

Ultra-thin aluminum (Al) and tin (Sn) films were grown by dc magnetron sputtering on a glass substrate. The electrical resistance R of films was measured in-situ method during the film growth. Also transmission electron microscopy (TEM) study was carried out to observe the microstructure of the films. In the ultra-thin film study, an exact determination of a coalescence thickness and a continuous film thickness is very important. Therefore, we tried to measure the minimum thickness for continuous film (dmin) by means of a graphical method using a number of different y-values as a function of film thickness. The raw date obtained in this study provides a graph of in-situ resistance of metal film as a function of film thickness. For the Al film, there occurs a maximum value in a graph of in-situ electrical resistance versus film thickness. Using the results in this study, we could define clearly the minimum thickness for continuous film where the position of minimum values in the graph when we put the value of Rd3 to y-axis and the film thickness to x-axis. The measured values for the minimum thickness for continuous film are 21 nm and 16 nm for sputtered Al and Sn films, respectively. The new method for defining the minimum thickness for continuous film in this study can be utilized in a basic data when we design an ultra-thin film for the metallization application in nano-scale devices.