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http://dx.doi.org/10.7847/jfp.2013.26.3.163

Histopathologic Characterization of Viral Pathogens in Cultured Olive Flounder, Paralichthys Olivaceus, using in-situ Hybridization Methods  

Do, Jeong Wan (Pathology Division, National Fisheries Research & Development Institute (NFRDI))
Lee, Nam-Sil (New Strategy Research Center, NFRDI)
Jung, Sung Hee (Pathology Division, National Fisheries Research & Development Institute (NFRDI))
Kim, Kyung-Kil (New Strategy Research Center, NFRDI)
Choi, Hye Sung (Pathology Division, National Fisheries Research & Development Institute (NFRDI))
Park, Jeong Woo (Department of Biological Sciences, University of Ulsan)
Kim, Yi Cheong (Aquaculture Industry Division, East Sea Fisheries Research Institute, NFRDI)
Publication Information
Journal of fish pathology / v.26, no.3, 2013 , pp. 163-171 More about this Journal
Abstract
Polymerase chain reaction (PCR) is the most rapid and widely used method to detect viral pathogens. However, this method does not provide histopathologic nature of the virus. In situ hybridization (ISH) with oligonucleotide probes is attractive because it is a rapid method for detection and identification of viral pathogens at sites of tissue infection. In order to understand the histopathologic characterictics of Red sea bream iridovirus (RSIV), viral-hemorrhagic septicemia (VHS) virus and viral nervous necrosis (VNN) virus to cultured olive flounder, we her applied ISH method to various kinds of olive flounder tissues with PCR-positive for these three viruses. We found that these viruses showed different tissue tropism and were detected from different cell types. Our results suggest that ISH is useful not only in rapid detection of viral pathogens but also in understanding the histopathologic characters of specific viral pathogens.
Keywords
Olive flounder; Paralichthys olivaceus; RSIV; VHSV; VNN; In-situ hybridization;
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