한국어병학회지 (Journal of fish pathology)

  • 제26권3호
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  • Pages.163-171
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  • 2013
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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In-situ hybridization 법을 사용한 양식 넙치, Paralichthys olivaceus의 바이러스 감염 질병 특성 고찰

Histopathologic Characterization of Viral Pathogens in Cultured Olive Flounder, Paralichthys Olivaceus, using in-situ Hybridization Methods

  • 도정완 (국립수산과학원 병리연구과) ;
  • 이남실 (국립수산과학원 전략연구단) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 김경길 (국립수산과학원 전략연구단) ;
  • 최혜승 (국립수산과학원 병리연구과) ;
  • 박정우 (울산대학교 생명과학부) ;
  • 김이청 (국립수산과학원 동해연구소 해역산업과)
  • Do, Jeong Wan (Pathology Division, National Fisheries Research & Development Institute (NFRDI)) ;
  • Lee, Nam-Sil (New Strategy Research Center, NFRDI) ;
  • Jung, Sung Hee (Pathology Division, National Fisheries Research & Development Institute (NFRDI)) ;
  • Kim, Kyung-Kil (New Strategy Research Center, NFRDI) ;
  • Choi, Hye Sung (Pathology Division, National Fisheries Research & Development Institute (NFRDI)) ;
  • Park, Jeong Woo (Department of Biological Sciences, University of Ulsan) ;
  • Kim, Yi Cheong (Aquaculture Industry Division, East Sea Fisheries Research Institute, NFRDI)
  • 투고 : 2013.07.29
  • 심사 : 2013.09.26
  • 발행 : 2013.12.31
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초록

PCR (polymerase chain reaction) 법은 신속하고 정확하여 바이러스성 질병진단을 위해 널리 사용되지만 조직병리학적인 정보를 제공하지 못한다. 반면에 in-situ hybridization (ISH) 법을 사용하면 바이러스를 빠르게 검출할 수 있을 뿐 아니라 조직에서의 분포도 알 수 있다. 본 연구에서는 RSIV, VHSV, 그리고 VNN 바이러스들의 조직내 분포 및 조직 병리학적인 특성을 확인하기 위하여 이 바이러스들에 감염된 에 감염된 양식 넙치의 어류의 다양한 조직들을 대상으로 ISH 법을 적용하였다. 그 결과 이들 세 종류의 바이러스가 각각 다른 조직 및 세포들에 감염함을 확인 할 수 있었다. 본 연구 결과는 ISH법이 어류 병원성 바이러스의 신속 검출 뿐 아니라 조직 병리학적인 특성 확인에도 유용함을 제시한다.

Polymerase chain reaction (PCR) is the most rapid and widely used method to detect viral pathogens. However, this method does not provide histopathologic nature of the virus. In situ hybridization (ISH) with oligonucleotide probes is attractive because it is a rapid method for detection and identification of viral pathogens at sites of tissue infection. In order to understand the histopathologic characterictics of Red sea bream iridovirus (RSIV), viral-hemorrhagic septicemia (VHS) virus and viral nervous necrosis (VNN) virus to cultured olive flounder, we her applied ISH method to various kinds of olive flounder tissues with PCR-positive for these three viruses. We found that these viruses showed different tissue tropism and were detected from different cell types. Our results suggest that ISH is useful not only in rapid detection of viral pathogens but also in understanding the histopathologic characters of specific viral pathogens.

키워드

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피인용 문헌

  1. A Study About Analysis Results for Kudoa septempunctata (Myxosporea: Multivalvulida) in Tissue at Olive Flounder, using PCR (polymerase chain reaction) and Histological Methods vol.35, pp.4, 2017, https://doi.org/10.11626/KJEB.2017.35.4.468
  2. Examination about Infection form of Kudoa septempunctata in muscle of olive flounder (Paralichthys olivaceus) using molecular and morphological methods vol.31, pp.1, 2013, https://doi.org/10.13000/jfmse.2019.2.31.1.154