• 동의생리병리학회지
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• 제26권2호
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• pp.166-174
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• 2012

To determine the effects of Mahaengeuigam-tang(MHEGT) on obesity, the obesity-related factors (gastrin, CGRP, ghrelin, glucagon-like peptide-1, insulin, orexin, leptin, serotonin, NPY) were investigated in the stomach, pancreas, brain of mice by immunohistochemical methods for 4 weeks after Mahaengeuigam-tang(MHEGT) administration. The change of boy weight decreased in MHEGT administered group than that of control group. The immunohistochemical density of the gastrin and CGRP positive cells on pylorus of stomach increased in MHEGT administered group than that of control group. The number of ghrelin immunoreactive cells on stomach decreased in MHEGT administered groups than that of control group. The immunohistochemical density of GLP-1 in the pancreas decreased in MHEGT administered group than that of control group. The immunohistochemical density of insulin positive cells in the pancreas decreased in MHEGT administered group than that of control group. The immunohistochemical density of orexin and NPY positive neurons in the diencephalon was slightly stronger in MHEGT administered group than that of control group. The immunohistochemical density of serotonin and leptin positive neurons was stronger in MHEGT administered group than that of control group. These results demonstrate that Mahaengeuigam-tang(MHEGT) increased the immunohistochemical density of factors related to appetite inhibitors, and decreased the immunohistochemical density of factors related to stimulator of food intake in stomach, pancreas and brain.

• 대한소아치과학회지
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• 제23권2호
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• pp.559-574
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• 1996

The purpose of this study was to determine whether there was any correlation between temporomandibular joint dysfunction and structure of the mandibular condyle. Weanling rats had their masseter muscles resected and immunohistochemical findings were observed with a light microscope. The results obtained were as follows : 1. The condylar cartilage region was divided into articular, proliferating, cartilage cell and hypertrophic cell layers according to cell morphology. 2. In light microscopic views, the proliferating and cartilage cell layers of the experimental group decreased gradually and at the 8th week significantly. 3. In immunohistochemical staining for type I and II collagen, a reaction was detected in the lower part of proliferating cell and cartilage cell layers. In the cartilage cell layers, a stronger cellular reaction was present. Immunohistochemical staining for type II collagen reacted more strongly than that of type I collagen. 4. In immunohistochemical staining for proteoglycan, the staining of the experimental group resembled the control group and gradually showed a weak reaction. The proliferating and cartilage cell layers reacted more strongly than the hypertrophic cell layer. 5. In immunohistochemical staining for proliferating cell nuclear antigen(PCNA), the strong reaction was detected in the nucleus of the proliferating cell layer both in control and experimental groups. But the thickness of the proliferating layer decreased in experimental group, consequently the reaction of the experimental group was reduced more than that of the control group.

• Journal of Korean Neurosurgical Society
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• 제41권6호
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• pp.367-370
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• 2007

Objective: Pituitary adenomas are common neurological lesions believed to account for 10% to 15% of all primary brain tumors. There can be diagnostic confusion due to discordance of the preoperative endocrine and the postoperative immunohistochemical diagnosis. In this study, the rate of discordance between preoperative and postoperative findings and their clinical implications were investigated. Methods: From March 2005 to March 2006, 26 patients who underwent surgery for a pituitary adenoma were enrolled in this study. The preoperative pituitary hormone level and postoperative immunohistochemical results were compared and analyzed. Results: The median age of the patients was 38 years [range 15-66 years]. The male to female ratio was 8 to 18. The endocrine evaluation showed 16 hormonally-active and 10 hormonally-inactive adenomas. The immunohistochemical findings showed : 13 prolactin-positive, 1 GH-positive, 1 FSH-positive, 8 pleurihormone-positive and 3 stain-negative adenomas. The percentage of discordance observed between the preoperative endocrine and postoperative immunohistochemical diagnosis was 54%. Nine of 10 endocrine non-functioning adenomas showed : 3 PRL positive, 1 GH positive, 2 PRL+GH positive, 1 TSH+FSH positive, 1 FSH+ACTH+PRL positive and 1 FSH+LH+PRL positive adenomas by immunohistochemistry. Three endocrine PRL+GH secreting adenomas showed 2 PRL positive and 1 FSH+GH positive by immunohistochemistry. One endocrine PRL secreting and 1 GH secreting adenoma showed 1 PRL+ TSH positive and 1 GH+PRL positive by immunohistochemistry, respectively. The diagnosis of the other 12 pituitary adenomas showed concordance. Conclusion : The results of this study showed 54% discordance rate between the preoperative endocrine and postoperative immunohistochemical diagnosis for pituitary adenomas.

• 대한핵의학회지
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• 제38권6호
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• pp.511-515
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• 2004

목적: 갑상선세포에서 요오드의 섭취는 갑상선호르몬 합성의 첫 단계이며, sodium iodide symporter (NIS)라는 세포막 단백질에 의해 이루어지는 것으로 알려져 있고 NIS 유전자가 클로닝됨으로써 NIS 발현을 직접 관찰하는 것이 가능해졌다. 하지만, 갑상선암을 비롯한 갑상선 결절과 정상 조직에서의 NIS 발현은 검사방법과 대상에 따라 아주 다양한 결과를 보이고 있다. 따라서 이 연구에서는 갑상선암을 비롯한 여러 갑상선질환에서 NIS의 발현여부와 정도를 두 가지 서로 다른 RT-PCR방법과 면역조직화학염색법으로 조사하여 비교하였다. 대상 및 방법: 갑상선절제술을 받은 32명의 조직을 이용하였다. 술후 병리학적 진단은 유두암 19명, 여포암 1명, 수질암 1명, 선종 4명, 선종양 갑상선종 7명이었다. RT-PCR방법을 이용하여 갑상선글로불린, NIS, 갑상선과산화효소의 발현을 관찰하였고, 항NIS 항체를 이용하여 면역조직화학염색을 시행하여 NIS 발현 정도를 반정량적으로 평가하고, 그 결과를 각각 비교하였다. 결과: RT-PCR의 결과에서 유두암 19명중 10예에서 NIS의 발현이 있었다. 여포암 1예와 수질암 1예는 NIS 발현이 없었다. 선종 4명중 2예, 선종양 갑상선종 7명중 4예에서 NIS의 발현이 있었다. 면역조직화학염색법으로는 유두암 19명중 15예에서 발현이 있었고, 여포암 1예는 발현이 없었다. 선종 4예중 3예, 선종양 갑상선종 7예중 6예에서 발현이 있었다. RT-PCR방법에 의한 NIS의 발현 정도와 면역조직화학염색의 정도를 반정량적으로 평가하여 비교한 결과 각 검사의 결과 사이에 유의한 양의 상관관계가 있었다(p<0.001). 결론: RT-PCR방법과 면역조직화학염색법으로 조사한 NIS의 발현 정도는 양의 상관관계가 있었으나, RT-PCR방법에 비해 면역조직화학염색법으로 NIS발현을 더 많이 찾을 수 있었다.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.518-528
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• 1995

The immunohistochemical study has been performed on the distribution of receptors for various growth factors in the newly forming granulation tissues following the guided tissue regeneration procedures. Two specimens from 2 different patients were collected from the newly forming granulation tissues at 2 weeks following GTR procedures using Gore-tex menbrane and rubber dam, respectively. For immunohistochemical localization of each recptor, anti-platelet-derived growth factor $receptor-{\alpha}$, anti-platelet-derived growth factor $receptor-{\beta}$. anti-insulin-like growth factor receptor, anti-basic fibroblast growth factor receptor, anti-transforming growth $factor-{\beta}$ receptor and anti-fibronectin receptor were incubated onto the specimens as primary antibodies. After the reaction, FITC-conjugated second antibodies have been applied. When the total numbers of immunoreactive cells and the true positive cells were counted, there were high variability among receptors tested in the present study. The mean number of immunoreactive cells were highest in the case for anti-IFG-1 receptor. However the number of true positive cells were highest in the case for $TGF-{\beta}$ receptor. The present investigation indicated that the receptor for $TGF-{\beta}$ were stongly expressed in the newly forming granulation tissues following the guided tissue regeneration therapy.

• 한국동물위생학회지
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• 제13권1호
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• pp.32-43
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• 1990

In order to investigate the clinico-pathological and immunohistochemical changes in the rats infected with Aujeszky’s disease virus(ADV), 100 heads of 4 weeks-old rats were inoculated intraperitoneally and intranasally, with the domestically isolated ADV, NYJ-1-87 strain, at $10^{3.0}$ or $10^{5.0}$$TCID_ {50}$/0.2ml. Results obtained through the experiments were summarized as follows : 1. Clinical signs such as dulness, anorexia, pruritus, fascial edema, dyspnea and ataxia were observed from the 2nd day and died at the 3rd to 5th day after ADV inoculation. By necropsy, congestion and hemorrhage were observed in the abdominal organs, while no specific changes were detected in the other organs. 2. In histopathological observation, degeneration and necrosis of the nervous cells, non-suppurative meningoencephalitis, microgliosis and perivascular cuffing were manifested in central nerve system but no specific changes were observed in the other organs. 3. By immunohistochemical staining using peroxidase antiperoxidase, the positive cells were detected in the tissues of kideny, spleen, urinary bladder and lung.

• 대한수의학회지
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• 제34권2호
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• pp.349-359
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• 1994

To elucidate pathogenicity to hamster of encephalomyocarditis virus $K_3$ strain that was isolated in Korea from the swine with reproductive failures, adult male syrian hamsters were experimentally infected intraperitoneally with the virus at $10^{7.0}\;TCID_{50}/0.1ml$ and pathological and immunohistochemical studies were performed. The results obtained through the experiment were as follows. 1. Clinical signs such as depression, unkempt hair and bilateral parlysis of hind limbs were observed. 2. At necropsy, mild congestion was observed in the cerebrum, liver, kidney and lung, and atrophy was evident in testis. 3. In microscopic observation, degeneration and necrosis of the nervous cells and perivascular mononuclear cell infiltration were manifested in central nerve system, and various degrees of degeneration and necrosis of parenchymal cells were detected in pancreas, lacrimal gland, liver, kidney and testis. 4. In immunohistochemical observation, strong positive reactions were observed in degenerated parenchymal cell of testis, and weak positive reactions, in hepatocytes.

• Applied Microscopy
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• 제21권1호
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• pp.46-62
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• 1991

The intermediate filament is one of the most important constituents of the intracytoplasmic cytoskeleton microtubule, actin, myosin and intermediate filament. It is composed of keratin, desmin, vimentin, neurofilament and glial filament, and has important role as a cellular marker, epithelial or mesenchymal origin. So it will be important to differentiated from some poorly or undifferentiated neoplasm to provide adequate therapeutic modalities. This study was performed by using immunohistochemical staining and electron microscopic observation to find out intermediate filaments of epithelial and non-epithelial tumor cells evaluate the degree of differentiation in tumors and therefore to provide some diagnostic and therapeutic modalities. The materials consisted of 83 epithelial and non-epithelial elements bearing 23 normal control, 28 epithelial tumors, and 32 non-epithelial tumors, that are resected for definite treatment at Chosun University Hospital from June, 1988 to June, 1990. Immunohistochemical stain for keratin, desmin and vimentin, and electron microscopic study were performed in all cases. The results obtained were as follows. 1. Immunohistochemical stain for intermediate filament were very useful diagnostic aid for differentiated epithelial tumor to non-epithelial tumor in diagnostic neoplasia. 2. In the electron microscopic finding, the size of intermediate filaments were possible differentiated to cell components of epithelial tumor and non-epithelial tumors.

• 한국동물위생학회지
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• 제17권1호
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• pp.67-80
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• 1994

8-10 week old ICR mice were infected intracerebrally and intraperitoneally with different encephalomyocarditis virus(K$_3$, $K_{11}$, ATT-VR 129) to observe histopathological and immunohistochemical change. Results obtained throuh the experiments were summarized as follows : 1. No differences in clinical signs by the virus strains and the inoculation routes were found. Mice infected with EMCV showed clinical signs after 3 days of inoculation. Main clinical signs were tremors, convulsions, circling movement, and uni or bilateral hindleg paralysis followed by death on the 3-8 days. In general, most of the infected animals died or recovered closely on the 8th day of postinoculation. 2. At necropsy, petechial and ecchymotic hemorrhages in lung were observed and no specific findings in other were observed. 3. In histopathological observation, neuroal cell degeneration perivascular mononucear cell in-filtration gliosis were appeared in central nervous system. Myocarditis with myocardial degeneration and necrosis, calcification were observed along with acinar cell necrosis of exocrine glands in pancreas, severe glomerulonephritis in kidney. Also, focal necrosis of hepatocytes and interstitial pneymonia hyperemia, hemorrhages in lungs were observed. 4. By immunohistochemical staining using ABCIT method, the positive cells were recognized in intracytoplasm of acinar cell in pancreas and intracytoplasm of neuronal cells in cerebrum.

• 한방안이비인후피부과학회지
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• 제27권4호
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• pp.87-100
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• 2014

Objectives : This study is aimed to analyse immunohistochemical research trend on psoriasis and consider the results in terms of Korean medicine. Methods : We analyse both immunohistochemical research papers and Korean medical papers on psoriasis, which I searched using KISS(Korean studies Information Service System) and OASIS(Oriental Medicine Advanced Searching Integrated System). Results : Psoriasis is characterized by hyperporliferation of the epidermis, inflammatory cell accumulation and increased tortuosity and dilatation of dermal papillary blood vessels. Recently, angiogenesis is considered to play a major role in pathogenesis of psoriasis. Conclusion : In Korean medicine, blood-tonifying, blood-cooling and blood-activating medicinal herbs have generally been used to treat psoriasis. I consider these herbs which also treat vessel problem can have an effect on angiogenesis of psoriasis.