Browse > Article

Expression of Sodium-Iodide Symporter (NIS) in Thyroid Nodules: Comparison of RT-PCR and Immunohistochemical Staining Methods  

Bae, Sang-Kyun (Department of Nuclear Medicine, Inje University College of Medicine)
Lee, Kang-Dae (Department of Head & Neck Surgery, Kosin University College of Medicine)
Chang, Hee-Kyung (Department of Pathology, Kosin University College of Medicine)
Publication Information
The Korean Journal of Nuclear Medicine / v.38, no.6, 2004 , pp. 511-515 More about this Journal
Abstract
Purpose: The sodium-iodide symporter (NIS) expression is an important factor in determining the sensitivity of radioiodine therapy in well-differentiated thyroid cancers. Several previous studies for the expression of NIS in thyroid tissues show diverse results. To investigate whether there is difference between methods in determining the expression of NIS in thyroid tissues of patients with thyroid nodules, we measured the expression ot NIS using two different methods (RT-PCR and immunoshistochemical staining) and compared the results. Materials & Methods: We measured the expression of NIS by reverse transcriptase-polymerase chain reaction (RT-PCR) and also by immunohistochemical staining using anti-NIS antibody in thyroid cancers and other benign thyroid diseases. We compared the results of each method. We included 19 papillary carcinomas, 1 follicular carcinoma, 7 medullary carcinoma, 4 adenomas and 7 nodular hyperplasias. Results: By RT-PCR analysis, 10 of 19 papillary carcinomas expressed NIS, but 1 follicular cancer didn't express NIS. By immunohistochemical staining, 15 of 19 papaillary carcinomas express NIS, but 1 follicular lancer didn't express NIS. There was a significant correlation between the semiquautitative results of RT-PCR and immunohistochemical staining of NIS expression. (p<0.01) Conclusion: Our data demonstrated that the expression of NIS in thyroid cancers and other benign diseases investigated by RT-PCR and immunohistochemical staining correlated well each other. However, by immunohistochemical staining, more NIS expression was found.
Keywords
Sodium-iodide symporter; RT-PCR; Immunohistochemical stain;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Arturi F, Russo D, Schlumberger M, du Villard JA, Caillou B, Vigneri P, et al. Iodide symporter gene expression in human thyroid tumors. J Clin Endocrinol Metab 1998;83:2493-6   DOI   ScienceOn
2 Ryu KY, Senokozlieff ME, Smanik PA, Wong MG, Siperstein AE, Duh QY, et al. Development of reverse transcription-competitive polymerase chain reaction method to quantitate the expression levels of human sodium iodide symporter. Thyroid 1999;9:405-9   DOI   ScienceOn
3 Park HJ, Kim JY, Park KY, Gong G, Hong SJ, Ahn IM. Expressions of human sodium iodide symporter mRNA in primary and metastatic papillary thyroid carcinomas. Thyroid 2000;10:211-7   DOI   ScienceOn
4 Smanik PA, Ryu KY, Theil KS, Mazzaferri EL, Jhiang SM. Expression, exon-intron organization, and chromosome mapping of the human sodium iodide symporter. Endocrinology 1997;138:3555-8   DOI   ScienceOn
5 Mian C, Lacroix L, Alzieu L, Nocera M, Talbot M, Bidart JM, et al. Sodium iodide symporter and pendrin expression in human thyroid tissues. Thyroid 2001;11:825-30   DOI   ScienceOn
6 Liou MJ, Lin JD, Chan EC, Liu FH, Chao TC, Weng HF. Detection of mrna of sodium iodide symporter in benign and malignant human thyroid tissues. Cancer Lett 2000;160:75-80   DOI   ScienceOn
7 Saito T, Endo T, Kawaguchi A, Ikeda M, Nakazato M, Kogai T, et al. Increased expression of the $Na^+/I^-$ symporter in cultured human thyroid cells exposed to thyrotropin and in Graves' thyroid tissue. J Clin Endocrinol Metab 1997;82:3331-6   DOI   ScienceOn
8 Kaminsky SM, Levy O, Salvador C, Dai G, Carrasco N. $Na^+/I^-$ symport activity is present in membrane vesicles from thyrotropindeprived non-I(-)-transporting cultured thyroid cells. Proc Natl Acad Sci U S A 1994;91:3789-93   DOI   ScienceOn
9 Dulgeroff AJ, Hershman JM. Medical therapy for differentiated thyroid carcinoma. Endocr Rev 1994;15:500-15   PUBMED
10 Goldman JM, Line BR, Aamodt RL, Robbins J. Influence of triiodothyronine withdrawal time on $^{131}I$ uptake postthyroidectomy for thyroid cancer. J Clin Endocrinol Metab 1980;50:734-9   DOI   PUBMED
11 Saito T, Endo T, Kawaguchi A, Ikeda M, Katoh R, Kawaoi A, et al. Increased expression of the sodium/iodide symporter in papillary thyroid carcinomas. J Clin Invest 1998;101:1296-300   DOI   ScienceOn
12 Castro MR, Bergert ER, Beito TG, McIver B, Goellner JR, Morris JC. Development of monoclonal antibodies against the human sodium iodide symporter: Immunohistochemical characterization of this protein in thyroid cells. J Clin Endocrinol Metab 1999;84:2957-62   DOI   PUBMED
13 Ringel MD, Anderson J, Souza SL, Burch HB, Tambascia M, Shriver CD, et al. Expression of the sodium iodide symporter and thyroglobulin genes are reduced in papillary thyroid cancer. Mod Pathol 2001; 14:289-96   DOI   ScienceOn
14 Caillou B, Troalen F, Baudin E, Talbot M, Filetti S, Schlumberger M, et al. $Na^+/I^-$ symporter distribution in human thyroid tissues: An immunohistochemical study. J Clin Endocrinol Metab 1998;83:4102-6   DOI   PUBMED
15 Schlumberger M, Charbord P, Fragu P, Gardet P, Lumbroso J, Parmentier C, et al. Relationship between thyrotropin stimulation and radioiodine uptake in lung metastases of differentiated thyroid carcinoma. J Clin Endocrinol Metab 1983;57:148-51   DOI   PUBMED
16 Smanik PA, Liu Q, Furminger TL, Ryu K, Xing S, Mazzaferri EL, et al. Cloning of the human sodium lodide symporter. Biochem Biophys Res Commun 1996;226:339-45   DOI   PUBMED   ScienceOn
17 Lazar V, Bidart JM, Caillou B, Mahe C, Lacroix L, Filetti S, et al. Expression of the $Na^+/I^-$ symporter gene in human thyroid tumors: A comparison study with other thyroid-specific genes. J Clin Endocrinol Metab 1999;84:3228-34   DOI   PUBMED
18 Spitzweg C, Joba W, Schriever K, Goellner JR, Morris JC, Heufelder AE. Analysis of human sodium iodide symporter immunoreactivity in human exocrine glands. J Clin Endocrinol Metab 1999;84:4178-84   DOI   PUBMED
19 Castro MR, Bergert ER, Goellner JR, Hay ID, Morris JC. Immunohistochemical analysis of sodium iodide symporter expression in metastatic differentiated thyroid cancer: Correlation with radioiodine uptake. J Clin Endocrinol Metab 2001;86:5627-32   DOI   ScienceOn
20 Kogai T, Endo T, Saito T, Miyazaki A, Kawaguchi A, Onaya T. Regulation by thyroid-stimulating hormone of sodium/iodide symporter gene expression and protein levels in FRTL-5 cells. Endocrinology 1997;138:2227-32   DOI   ScienceOn
21 Jhiang SM, Cho JY, Ryu KY, DeYoung BR, Smanik PA, McGaughy VR, et al. An immunohistochemical study of $Na^+/I^-$ symporter in human thyroid tissues and salivary gland tissues. Endocrinology 1998;139:4416-9   DOI   ScienceOn
22 Spitzweg C, Heufelder AE. Update on the thyroid sodium iodide symporter: A novel thyroid antigen emerging on the horizon. Eur J Endocrinol 1997;137:22-3   DOI   ScienceOn
23 Dai G, Levy O, Carrasco N. Cloning and characterization of the thyroid iodide transporter. Nature 1996;379:458-60   DOI   ScienceOn
24 Trouttet-Masson S, Selmi-Ruby S, Bernier-Valentin F, Porra V, Berger-Dutrieux N, Decaussin M, et al. Evidence for transcriptional and posttranscriptional alterations of the sodium/iodide symporter expression in hypofunctioning benign and malignant thyroid tumors. Am J Pathol 2004;165:25-34   DOI   PUBMED   ScienceOn
25 Castro MR, Bergert ER, Beito TG, Roche PC, Ziesmer SC, Jhiang SM, et al. Monoclonal antibodies against the human sodium iodide symporter: Utility for immunocytochemistry of thyroid cancer. J Endocrinol 1999; 163:495-504   DOI   ScienceOn
26 Min JJ, Chung JK, Lee YJ, Jeong JM, Lee DS, Jang JJ, et al. Relationship between expression of the sodium/iodide symporter and 131I uptake in recurrent lesions of differentiated thyroid carcinoma. Eur J Nucl Med 2001;28:639-45   DOI   ScienceOn