• 한국식품위생안전성학회지
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• 제16권3호
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• pp.200-205
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• 2001

시중 유통중인 유 및 유제품의 AFM$_1$의 오염수준을 파악하기 위해 분석한 결과 우유의 경우는 원유(0.083 ppb)에 비해 시유의 AFM$_1$농도(0.047 ppb)가 낮게 나타났으나 이는 살균의 효과라기보다 집유 과정에서의 희석 때문인 것으로 보이며, 탈지분유의 경우 AFM$_1$의 농도가 0.24 ppd로 높게 나타났으나 섭취시 희석되므로 우유와 큰 차이는 없을 것으로 생각되었다. 아이스크림제품의 경우 AFM$_1$오염농도는 0.020 pub로 나타났다. 이상의 결과는 미국 FDA의 허용기준치인 0.5 ppb보다 월등히 낮은 수치로 나타난 것이다. 국내 시판 우유 중 AFM$_1$오염수준을 평가하기 위해 본 연구의 결과와 기존의 국내 연구 결과를 토대로 Monte-Carlo 시뮬레이션을 시행하였다. 불확실성과 다양성을 고려하기 위해 fitting 절차를 거쳤으며, 시판 우유 중 AFM$_1$의 발생정도(prevalence)를 추정하기 위해 beta distribution을, 우유에서의 AFM$_1$오염농도(concentration)를 추정하기 위해 triangular distribution을 적용한 결과, 국내 시판 우유에서 AFM$_1$의 발생 가능한 오염수준(contamination level)은 최소(5% percentile) 0.0214, 평균(50% percentile) 0.0946 및 최대(95% percentile) 0.1888 ppb로 나타났다. 전체적으로 미국의 AFM$_1$허용기준치인 0.5 ppb보다는 상당히 낮은 수준이지만 시판우유의 80.4%근 유럽의 기준인 0.05ppb보다 높은 수준으로 예측되어 유럽의 허용기준치를 초과하는 것으로 나타났다.

• 한국식품영양학회지
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• 제28권1호
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• pp.47-59
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• 2015

In this study, we investigated the dietary habits, snacks, and self-purchasing snacks (SPS) intake behaviors of 519 elementary school students (boys=239, girls=280). Obesity was significantly higher (p<0.05) in boys (24.8%) than in girls (14.7%) and the proportion of underweight subjects was higher compared to normal or other weight groups for both the boys and the girls. There were 7.5% of the subjects in the group that always skipped breakfast, and the main reason of skipping breakfast was insufficient time (51.9%). The snack intake frequency was once or twice per week for 23.1% of the subjects and three or four times per week for 25.1%. The SPS intake frequency was the subjects zero for 35.6% of the highest level of the responders, while 6.8% of the respondents took more than once SPS per day. 59.6% of the respondents consumed SPS due to hunger while 15.0% consumed SPS out of boredom. The SPS was purchased from supermarkets in 34.5% of the cases, from convenience stores in 24.1% and from snack corners in 20.0% of cases or from a store near school in 14.5% of the cases. Analysis of SPS behaviors according to obesity index showed that parent's opinion of 'permission to buy SPS as needed' had a significant effect in 64.5% over weight subjects compared to only 53.7% in underweight groups. The subjects who used more than 3/4 of their pocket money to buy SPS was higher in the overweight groups (16.4%) than in the underweight groups (7.0%) and normal weight groups (9.8%). The favorite snacks and SPS were milk and yogurt for 45.7% of the subjects, fruits for 42.7%, ice cream for 26.4%, fruit juices for 23.8%, sweet stuff for 16.4%, frozen dessert for 8.9%, and chocolate or candy for 8.1% in descending order. The intake frequency of milk, yogurt, and fruit juices was higher in the underweight groups, but the intake frequency of sweet stuff, frozen dessert, and chocolate or candy was higher in over weight groups. The intake of frozen dessert was more than four times higher in the overweight groups than in the underweight groups. In conclusion, dietary habits, snacks, and SPS intake behaviors were similar between the boys and the girls and obesity groups, but most students appeared to have a high preference for intake snacks and SPS. Therefore, education for appropriate snacks intake habits will be beneficial for improving their dietary habits and health.

• 대한가정학회지
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• 제26권3호
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• pp.53-68
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• 1988

This article concerned with the nutritional status and the taste of the lunch box of 311 middle school girls in Kwangju City from May 18 to June 12 in 1987. The purpose of this research was making materials to show direction of the education about nutrition by checking relationships among the nutritional status for the lunch box, the taste of food and the environmental factors. The results observed in the study were as follows: 1. The intake of nutrition from the lunch box and the ratio between the recommended dietary allowance and the contained nutrients in the lunch box as follows. Calorie(603 Kcal, 78.6%), protein (21.7g, 93.1%) Animal protein (10.3g, 132.1%) calcium (151.7 g, 56.9%) Ferrum 93.1 mg, 51.7%), Vitamin a (129.3 RE, 55.4%) Vitamin B1 (0.29 mg, 72.5%), Vitamin B (0.26 mg, 55.3%) Niacin (4.7 mg, 94.0%), Vitamin C 913.2 mg, 79.0%). Except animal protein, all the recommended dietary allowance. 2. According to the intake of calorie, the ratio of taken carbohydrate, protein and fat was 77.7 : 15.5 : 6.8. The intake of protein was desirable but most of calorie depended on carbohydrate. Among the total intake of protein, the ratio of animal protein was 47.5%, which was a high rate. 3. Most of calorie (75%), was taken from staples, protein (41.5%), vitamin B1 (48.3%) were taken at the same rate from staples and side dishes. Most of fat, calcium, ferrum, vitamin A, vitamin B, and vitamin C, was taken from side dishes. 4. In taking among the five basic food groups, proportion of the grain fourth food group was the highest, light color vegetable the third food groups, proportion of the grain fourth food group was the highest, light color vegetable the third food group was second, and Calcium food the second food group was the lowest. 5. As the staples, students liked tchajangmyon, mandu and ttokkuk as written order. They disliked Kongbap and Patpap. As the side dishes for the lunch box, they liked kimchi, ham, sausage, cuttlefish, dried slices of filefish, eggs and green seaweed as written order. As a side dishes they liked Laver, Cucumber, Squid, lettuce, Potatoes. They disliked pork fat, cow's intestines, cow's liver, Crussian carp, pickled fish. The favorite snack was fruits, ice cream, hamburger, Chocolate and milk. 6. In taking condition of the principal food, rice rate (65.6%) was the most, and mixed food was 5 or 10%. 7. Favorite cooking was frying, roasting and kimch. But disagreeable cooking was pickling. 8. Favorite food was what was pungent but disagreeable food was what was salty. 9. the higher parents educational background was, the higher their income was. Also the more various the information about the nutrition was, the better the intake of nutrition was. 10. The preference tendency influenced in choosing the side dish of the Lunch box. The higher the preference tendency of the girl students became, the better their nutrition condition became.

• Asian-Australasian Journal of Animal Sciences
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• 제28권11호
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• pp.1649-1656
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• 2015

Gene expression profiling has offered new insights into postmortem molecular changes associated with meat quality. To acquire reliable transcript quantification, high quality RNA is required. The objective of this study was to analyze integrity of RNA isolated from chicken skeletal muscle (pectoralis major) and its capability of serving as the template in quantitative real-time polymerase chain reaction (qPCR) as a function of postmortem intervals representing the end-points of evisceration, carcass chilling and aging stages in chicken abattoirs. Chicken breast muscle was dissected from the carcasses (n = 6) immediately after evisceration, and one-third of each sample was instantly snap-frozen and labeled as 20 min postmortem. The remaining muscle was stored on ice until the next rounds of sample collection (1.5 h and 6 h postmortem). The delayed postmortem duration did not significantly affect $A_{260}/A_{280}$ and $A_{260}/A_{230}$ ($p{\geq}0.05$), suggesting no altered purity of total RNA. Apart from a slight decrease in the 28s:18s ribosomal RNA ratio in 1.5 h samples (p<0.05), the value was not statistically different between 20 min and 6 h samples ($p{\geq}0.05$), indicating intact total RNA up to 6 h. Abundance of reference genes encoding beta-actin (ACTB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine-guanine phosphoribosyltransferase (HPRT), peptidylprolylisomerase A (PPIA) and TATA box-binding protein (TBP) as well as meat-quality associated genes (insulin-like growth factor 1 (IGF1), pyruvate dehydrogenase kinase isozyme 4 (PDK4), and peroxisome proliferator-activated receptor delta (PPARD) were investigated using qPCR. Transcript abundances of ACTB, GAPDH, HPRT, and PPIA were significantly different among all postmortem time points (p<0.05). Transcript levels of PDK4 and PPARD were significantly reduced in the 6 h samples (p<0.05). The findings suggest an adverse effect of a prolonged postmortem duration on reliability of transcript quantification in chicken skeletal muscle. For the best RNA quality, chicken skeletal muscle should be immediately collected after evisceration or within 20 min postmortem, and rapidly preserved by deep freezing.

• Journal of Nutrition and Health
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• 제20권5호
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• pp.309-317
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• 1987

우리나라 아동의 비만실태를 파악하기 위한 연구의 일환으로 전국의 국민학교 5학년 10.751명의 체격분포를 조사하였다. 건강기록부를 이용하요 1학년때 부터 학년까의 키, 몸무게를 분석하고 설문지를 이용하여 체격과 관련된 일반 현황 및 식품섭취 빈도를 조사하였다 1)조사대상아의 어머니 중 29%가 직업을 갖고 있었으며 출, 퇴근시간이 어린이의 영양관리를 제대로 할 수 없는 경우가 많았다. 조사대상아의 5.8%가 거의 매일 결식하였으며 건강을 위해 약제를 복용하는 경우가 26%였다. 2)키는 4학년까지는 남학생이 우세 하다가 5학년때는 남학생이 137.1cm, 여학생이 137.6cm 였다. 몸무게는 남녀 각각 31.5kg, 31.2kg였으며 키, 몸무게의 10, 25, 50, 70 ,90percentile 분포에 해당하는 값이 제시되었다. 고학년이 될수록 남학생의 키의 성장속도는 둔화된 반면 여학생은 오히려 증가였다. 몸무게는 학년이 높아지면서 남녀 모두 증가폭이 커졌으며 여학생의 증가폭이 더 컸다. 키와 몸무게를 이용하여 5년간 BMI, RI, RW 갑이 계산되었다. 3) 49% 아동이 우유를 매일 섭취 하였으며 과자, 계란, 아이스크림, 초코렛의 섭취빈도가 높은 반면 치즈, 햄, 두유의 섭취빈도는 매우 낮았다.

• 동아시아식생활학회지
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• 제24권3호
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• pp.315-324
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• 2014

The purpose of this study was to investigate the dietary behaviors, eating habits, and nutrient intakes of elementary students with atopic dermatitis. Dietary conditions during infancy, dietary regularity, dietary behaviors, eating habits, and daily nutrient intakes were surveyed and compared between children with (WAD) and without (WOAD) atopic dermatitis using the 24-hr recall method. The ratio of children who responded 'I eat regular meals' was significantly lower in the WAD compared to WOAD group (76.4% vs. 81.7%, p<0.05). The ratio of children who answered 'I eat processed foods or fast foods more than once a week' (p<0.01) and 'I take nutritional supplements' (p<0.001) was significantly higher in the WAD compared to WOAD group. The scores for 'I eat milk and its products everyday' (1.4 vs. 1.5, p<0.05), 'I eat dishes fried or stirred with oil more than twice a week' (2.0 vs. 2.1, p<0.05), and 'I eat snacks such as ice cream, cake, snacks, carbonated beverages more than twice a week' (1.9 vs. 2.0, p<0.05) were significantly lower in the WAD group than in the WOAD group. Daily energy intakes were 1,859.1 kcal and 1,829.5 kcal in WAD and WOAD children, respectively, with no significant difference. However, daily intakes of fat (p<0.05), phosphorus (p<0.05), and vitamin $B_2$ (p<0.05) in WAD children were significantly higher than in WOAD children. To sum up, elementary students with atopic dermatitis had irregular eating habits and higher intake frequency of processed or fast foods, milk and its products, dishes fried or stirred with oil, and snacks compared to normal children. Dietary guidance and nutritional education are required to improve irregular eating habits and induce adequate nutrient intakes in children with atopic dermatitis.

• Journal of Nutrition and Health
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• 제1권2호
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• pp.107-115
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• 1968

Number of aspects, not only nutritional but social as well as political involved in human starvation pose nowadays global problems. In order to help establish the minimum nutritional requirements in the daily life of a man and to free people as well from either undernourishment, malnutrition or even starvation many workers have devoted themselves so far on the research programs to know what and how number of metabolic events take place in animals in vivo. It is the purpose of the present paper to examine in effect to what extent both of the protein and nucleic acids (DNA & RNA) together with an enzyme, guanine deaminase, which converts guanine into xanthine and in turn ends up to uric acid as an end product, undergo changes, quantitatively during acute starvation, using the mouse as an experimental animal. The mouse was strictly inhibited from taking foods except drinking water ad libitum and was sacriflced 24, 48, and 72 hours following starvation thus acutely induced. The animals consisted of two experimental groups, one control and another starvation groups, each being consisted of 6-24 mice of whose body weights ranged in the vicinity of 10 g. The animals were sacriflced by a blow on the head, followed by immediate excision of their livers into ice-cold distilled water, washing adherent blood and other contaminant tissues. The liver was minced foramin, by an all-glass homogenizer immersing it in an ice-bath, followed by subsequent fractionatin of the homogenate (10% W/V in 0.25M sucrose solution made up with 0.05M phosphate buffer of pH 7.4). For the liver protein and guanine deaminase assay, the 10% homogenate was centrifuged at 600 x g for 10 minutes to eliminate the nuclear fraction; and for the estimation of DNA and RNA, the homogenate was prepared by the addition of 10% trichloroacetic acid in order to free the homogenate from the acid-soluble fraction, the remaining residue being delipidate by the addition of alcohol and dried in vacuo for later KOH (IN) hydrolysis. The changes in body and liver wegihts during acute starvation were checked gravimetrically. Protein contents in the liver were monitored by the method of Lowry et al; and guanine deaminase activities were followed by the assay of liberated ammonia from the substrate utilizing the Caraway's colorimetry. The extraction of both DNA and RNA was performed by the Schmidt-Thannhauser's method, which was followed by Marmur's method of purification for DNA and by Chargaff's method of purification for RNA. The determinations of both DNA and RNA were carried out by the diphenylamine reaction for the former and by the orcinol reaction for the latter. The following resume was the results of the present work. 1. It was observed that the body as well as liver weights fall abruptly during starvation, and that the loss of body weight showed no statistical correlation with the decreases in the content of liver protein. 2. The content of liver protein and activity of liver guanine deaminase activity as well decline dramatically, and the specific activities of the enzyme (activity/protein), however, decreased gradually as starvation proceeded. 3. Both of the nucleic acids, DNA and RNA, showed decrements in the liver of mouse during acute starvation; the latter, however, being more striking in the decline as compared to the former. 4. The decreases in the liver protein content as resulted from the acute starvation had no statistically significant correlation with the decrements of DNA in the same tissue, but had regressed with a significant statistical correlation with the fall of RNA in the tissue. 5. The decrease in the activity of guanine deaminase in the liver of mouse during acute starvation was functionally more proportional to the decrease in RNA than DNA, and moreover correlated with the changes in the content of the liver protein. 6. The possible mechanisms involved during in this acute starvation as bring the decreases in the contents of DNA, protein, and guanine deaminase were discussed briefly.

• Journal of Korean Dental Science
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• 제10권1호
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• pp.10-21
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• 2017

Purpose: The purpose of this study was to investigate the electrochemical characteristics of nanotubular Ti-25Nb-xZr ternary alloys for dental implant materials. Materials and Methods: Ti-25Nb-xZr alloys with different Zr contents (0, 3, 7, and 15 wt.%) were manufactured using commercially pure titanium (CP-Ti), niobium (Nb), and zirconium (Zr) (99.95 wt.% purity). The alloys were prepared by arc melting in argon (Ar) atmosphere. The Ti-25Nb-xZr alloys were homogenized in Ar atmosphere at $1,000^{\circ}C$ for 12 hours followed by quenching into ice water. The microstructure of the Ti-25Nb-xZr alloys was examined by a field emission scanning electron microscope. The phases in the alloys were identified by an X-ray diffractometer. The chemical composition of the nanotube-formed surfaces was determined by energy-dispersive X-ray spectroscopy. Self-organized $TiO_2$ was prepared by electrochemical oxidation of the samples in a $1.0M\;H_3PO_4+0.8wt.%$ NaF electrolyte. The anodization potential was 30 V and time was 1 hour by DC supplier. Surface wettability was evaluated for both the metallographically polished and nanotube-formed surfaces using a contact-angle goniometer. The corrosion properties of the specimens were investigated using a 0.9 wt.% aqueous solution of NaCl at $36^{\circ}C{\pm}5^{\circ}C$ using a potentiodynamic polarization test. Result: Needle-like structure of Ti-25Nb-xZr alloys was transform to equiaxed structure as Zr content increased. Nanotube formed on Ti-25Nb-xZr alloys show two sizes of nanotube structure. The diameters of the large tubes decreased and small tubes increased as Zr content increased. The lower contact angles for nanotube formed Ti-25NbxZr alloys surfaces showed compare to non-nanotube formed surface. The corrosion resistance of alloy increased as Zr content increased, and nanotube formed surface showed longer the passive regions compared to non-treatment surface. Conclusion: It is confirmed that corrosion resistance of alloy increased as Zr content increased, and nanotube formed surface has longer passive region compared to without treatment surface.

• Ocean and Polar Research
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• 제24권2호
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• pp.123-134
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• 2002

Seasonal variations of settling particles and metal fluxes were monitored at a nearshore site of Marian Cove, King Geroge Island, Antarctica from 28th February 1998 to 22nd January 2000. Near-bottom sediment traps were deployed at 30m water depth of the cove, and sampling bottles were recovered every month by SCUBA divers. Total particulate flux and metal concentrations were determined from the samples. Total particulate flux showed a distinct seasonality, high in austral summer and low in austral winter: the highest flux $(21.97g\;m^{-2}d^{-1})$ was found in February of 1999, and the lowest $(2.47g\;m^{-2}d^{-1})$ in September of 1998, when sea surface was frozen completely. Lithogenic particle flux accounted for 90% of the total flux, and showed a significantly negative correlation with the thickness of snow accumulation around the study site. It was suggested that the most of the lithogenic particles trapped in the bottles was transported by melt water stream from the surrounding land. Fluxes of Al, Fe, Ti, Mn, Zn, Cii, Co, Ni, Cr, Cd, and Pb showed similar seasonal variations with the total flux, and their averaged fluxes were 34000, 9000,960, 180, 13.8, 17.6, 3.0,2.1, 5.4, 0.02, and $1.5nmol\;m^{-2}d^{-1}$ respectively. Among the metals, Cu and Cd showed the most noticeable seasonal patterns. The Cd flux correlated positively with the fluxes of biogenic components while the Cu flux correlated with both the lithogenic and biogenic particle fluxes. The Cu flux peak in the late summer is likely related to a substantial amount of inflow of ice melt water laden with Cu-enriched lithogenic particles. On the other hands, the Cd flux peak in the early spring may be associated with the unusually early occurred phytoplankton bloom.

• 한국제4기학회지
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• 제8권1호
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• pp.9-22
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• 1994

홀로세 동안의 한반도 식생사와 환경변천사를 고식물상, 고기후, 고고학 고문헌등 다 양한 자료를 활용하여 복원하였다. 10,000년 전부터 6,700년전까지 중부 동해안 지방에는 온 대성 낙엽 활엽수가 점차 증가하였으며 이에 따라 서늘한 기후를 선호하는 나무들은 8,000 년 전을 기점으로 급격히 감소하였다 6,700년 전부터 4,500년 전에는 비교적 온난한 기후를 반영하는 참나무류와 잎이 2개인 소나무류등이 번성하여 약 8,000년전부터 4,500년전까지가 후빙기에 들어서 가장 따뜻했던 시기로 추측된다, 4,500년 전부터 1,400년 전까지 중부지방 에는 서늘한 기후를 선호하는 식물들이 그리고 남부 지방에는 온난한 기후를 좋아하는 식물 들이 각각 분포하여 식생과 기후의 남북차가 뚜렷했음을 알수 있다. 재배되는 벼의 화분과 초본식물의 꽃가루 및 포자의 출현은 인위적인 삼림 파괴가 커져감을 나타낸다. 1,400년 전 부터는 온대성 나무들이 급격히 감소하는 반면 서늘한 기후를 선호하는 전나무속이나 잎이 5개인 소나무 등이 출현하여 기후가 한랭해졌음을 나타내며 이는 아마도 소빙기의 출현과도 연관되어 있을것으로 본다. 한반도 중부 이남지방의 서해안과 동해안의 식생을 시, 공간적 측면에서 복원한 후 비교 검토하였다, 한반도 서해안의 경우 6,250년 전부터 1,500년 전까지 오리나무속이 우점하는 식생이 지속되었으나 1,500년 전부터는 소나무속이 이를 대치하였다 반면에 동해안에서는 10,000년 전에서 2,000년 전까지 참나무속과 소나무속이 주로 분포하여 으나 2,000년전부터는 소나무속이 우점하였다, 1425년 이래 기록된 고문헌들을 활용하여 역 사 시대 동안의 산지 식무과 재배 식물의 분포역 그리고 분포의 시대적 변천과정을 파악할 수 있었다.