• 한국식품영양과학회지
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• 제32권6호
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• pp.921-925
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• 2003

콩나물 메탄을 추출물의 yeast $\alpha$-glucosidase 저해활성을 in vitro에서 측정한 결과 5 mg/mL 농도에서 24.5%로 나타났다. 콩나물 용매분획 중 ethyl acetate층에서 $\alpha$-glucosidase 저해활성이 36.3%로 가장 높게 나타났다. STZ로 당뇨를 유발한 흰쥐에 있어서 콩나물 추출물의 탄수화물 소화효소 저해활성을 측정하였다. 공복상태의 동물에게 전분(1 g/kg)과 함께 콩나물 메탄올 추출물 (500 mg/kg)을 경구 투여한 경우, 전분(1 g/kg)만 투여 경우에 비해 투여 후 60, 90, 120, 180분에 혈당 증가가 유의적으로 낮았으며 (p<0.05), 식후 혈당 증가곡선의 면적(AUC)도 유의적으로 작게 나타났다(p<0.05). 이상의 결과에서 콩나물 메탄올 추출물은 $\alpha$-glucosidase 저해 활성에 의해 식후 혈당 조절 효과를 나타내어, 콩나물의 섭취는 당뇨병 치료 및 예방에 도움을 줄 수 있는 것으로 사료된다.

• Food Science and Biotechnology
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• 제17권1호
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• pp.130-134
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• 2008

Hypoglycemic effect of onion skin extract in vitro and in vivo was investigated. A methanol extract of onion skin inhibited yeast $\alpha$-glucosidase with an $IC_{50}$ of 0.159 mg/mL. A single oral administration of the onion skin extract (500 mg/kg) significantly lowered the postprandial area under the glucose response curve to starch (1 g/kg, p<0.05). Three-week-old db/db mice were fed an AIN-93G diet or a diet supplemented with a 0.5% onion skin extract for 7 weeks after a 1-week adaptation period. Consumption of onion skin extract significantly reduced the levels of plasma glucose, insulin, and blood glycated hemoglobin as compared with the control group (p<0.05). These findings suggest that onion skin is effective in controlling hyperglycemia in animal models of type 2 diabetes mellitus, at least in part by inhibiting $\alpha$-glucosidase activity.

• 한국식품조리과학회지
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• 제13권5호
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• pp.639-647
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• 1997

The hypoglycemic effects of five Korean wild vegetables, Aralia continentalis (A. con.), Castanea crenata (C. cre.), Xanthium strumarium (K, str.), Alisma canaliculatum (A. can,) and Eupatorium chinense var. simplicifolium for tripartium (E. tri) which have been utilized for the traditional remedies were investigated in this study. Diabetes mellitus was induced in male Sprague-Dawley rats by injections of streptozotocin (STZ) into the tail veins at a dose of 45 mg/kg. Five groups of STZ-induced diabetic rats were fed one of each experimental diet containing 10% of the Korean wild vegetable powder and normal and STZ-control rats were fed the control diet for five weeks. The body weight change, feed efficiency ratio (FER) and organ weights were compared. The plasma levels of glucose, protein, cholesterol, HDL-cholesterol, triglyceride, free fatty acid, and aminotransferase activity were determined. Mineral (Ca, K, Mg, Na, Cu, Fe, Mn and Zn) contents of the Korean wild vegetables were analyzed. The body weight gain was higher in normal, C. cre., A. can. and E. tri. groups than in the diabetic control group. The FER of C. cre., A. can. and E. tri. groups was significantly higher (p.＜0.05) than that of diabetic control group. Liver weight was heavier in A. con., X. str. and A. can. groups compared with the diabetic control group. The weights of kidney were lighter in all five Korean wild vegetable groups than in the diabetic control group. After five weeks, the plasma glucose level tends to be decreased in A. con., A. can. and E. tri. groups. Plasma cholesterol level was decreased the Korean wild vegetables except for X. str. group. Plasma HDL- cholesterol level was significantly higher in A. con., A. can. and E. tri. groups compared with the diabetic control group. Plasma triglyceride and free fatty acid levels were significantly higher in X. str. group compared with the diabetic control group. Mineral contents were higher in E. tri. (Ca, K, Na and Fe). The results suggest that the intakes of A. con., A. can. and E. tri. have a hypoglycemic effect in diabetic rats showing the possibility as the valuable food resources for the prevention of diabetic mellitus.

• Biomolecules & Therapeutics
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• 제5권4호
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• pp.364-368
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• 1997

Onion (Allium cepa Linn) has been reported to have hypoglycemic activity in human and several animal models. In the present study, we performed intraperitoneal glucose tolerance test (IPGTT) in young (1.5mo) and aged (5 mo) rats treated with onion in order to determine whether aging can influence on the anti-hy-perglycemic effect of onion. In addition, we investigated the hypoglycemic effect of onion in streptozotocin- induced diabetic rats treated with aqueous extracts of onion (500 mg/kg, i.p., daily) for 4 weeks. Blood glucose level was determined in fasted and fed rats by using a glucometer (Johnson & Johnson). In glucose tolerance test, blood glucose level was maximally increased 15 min after glucose load (2 g/kg, i.p.), and recovered to the basal level 3 hr after glucose challenge in young and old rats. The maximum blood glucose levels of young and aged rat were 184$\pm$7.49 and 225.2$\pm$ 12.55 mg/dl, respectively. A single i.p. injection of aqueous extract of onion (1 g/kg) 30 min before glucose challenge significantly decreased blood glucose levels at 15, 30, 60, 90 min after glucose load in aged rats, while the administration of onion did not show any significant effect in young rats. In onion-treated diabetic rats, significant hypoglycemic effect (p<0.05) was observed, and the effect was greater in fasted rats than in fed. In conclusion, these results suggest that anti-hyperlycemic effect of onion can be changed by age and fasting.

• 한국식품영양과학회지
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• 제34권6호
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• pp.821-824
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• 2005

누에 동충하초(Paecilomyces japonica) 섭취 후 공복혈당은 $27.5\%$, 식후혈당은 $35.5\%$로 감소하였으며, 복용 중단 2주 후에도 실험전 혈당 수준과 비교할 때 각각 $18.2\%,\;27.2\%$ 저하된 상태를 유지하고 있었다. 이는 복용할 때 뿐 아니라 복용 후 어느 정도 기간까지는 지속적으로 혈당 강하 효과가 있음을 의미한다. 남자 당뇨병 환자의 공복혈당은 $28.5\%$, 식후혈당은 $33.5\%$ 감소되었고, 여자 환자의 공복혈당은 $26.1\%$, 식후혈당은 $37.3\%$ 감소되었다. 이러한 결과로 볼 때 누에 동충하초는 인슬린비의존형 성인 당뇨병 환자의 혈당을 감소시키는데 유용한 기능성 식품으로 활용될 수 있을 것으로 판단된다.

• 대한본초학회지
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• 제32권4호
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• pp.47-52
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• 2017

Objective : Phellinus spp. mushroom is an object of interest because it has excellent anticancer effect. Owing to the similalarities in the morphology, Phellinus linteus and Phellinus baumii are often used as same Sang Hwang Mushroom in the Korean market.. The quality control for mushrooms is needed because there are many differences in the efficacy according to cultivation method and cultivation area. Therefore, a reliable authentication method of these herbal medicine is necessary to compare and measure the amount of beta-glucan which is known to have a hypoglycemic effect, from the mushrooms collected in various regions Methods : 7 samples of medicinal mushrooms supplying phellinus spp. were collected in Korea, China and Cambodia. We investigated the hardness, colors, extract ratio, ${\alpha}-amylase$ and ${\alpha}-glucosidase$ inhibitory activities, glucose transporter 4 (GLUT-4) expressions of water extracts from Phellinus spp and also MTT assay were examined for cell toxicity. Results : The results revealed that Phellinus spp.water Ext.inhibited ${\alpha}-glucosidase$ activity. glucose transporter 4 (GLUT-4), the key insulin signaling pathway transcription factor, was remarkably increased by the Phellinus baumii water extract Conclusions : These results suggest that The more yellowish the mushroom is, the lower the hardness, the more the content of ${\beta}-glucan$ is proportional. Because the more ${\beta}-glucan$, the greater the effect of hypoglycemia. compared to the hypoglycemic effect, Phellinus Baumii grown at hanging on selves for 7 month in the green house is the best.

• Nutrition Research and Practice
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• 제7권5호
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• pp.359-365
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• 2013

Anthocyanin from purple sweet potato (PSP) extracted by microwave baking (MB) and acidified electrolyzed water (AEW) exhibited antioxidant activity. After further purification by macroporous AB-8 resin, the color value of PSP anthocyanin (PSPA) reached 30.15 with a total flavonoid concentration of 932.5 mg/g. The purified extracts had more potent antioxidant activities than the crude extracts. After continuously administering the PSP extracts to 12-mo-old mice for 1 mo, the anti-aging index of the experimental group was not significantly different from that of 5-mo-old mice. To a certain degree, PSPA was also effective for controlling plasma glucose levels in male Streptozocin (STZ)-treated diabetic mice. In addition, the extracts inhibited Sarcoma S180 cell growth in ICR mice. Mice consuming the PSP extracts formed significantly fewer and smaller sarcomas than mice consuming the control diets. The highest inhibition rate was 69.03%. These results suggest that anthocyanin extracts from PSP not only exert strong antioxidant effects in vitro, but also had anti-aging, anti-hyperglycemic, and anti-tumor activities.

• 한국약용작물학회지
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• 제7권4호
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• pp.257-262
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• 1999

건지황과 숙지황의 에탄올 추출물에 대해 생리활성 실험을 하였는데 돌연변이 유발 억제실험에서는 MNNG의 돌연변이원성을 평가한 결과, 숙지황이 높은 억제활성을 나타내었다. 암세포 성장저해에서 건지황과 숙지황 모두 1 g/ l 의 농도에서 50%이상의 억제율을 보였고 특히, 숙지황은 1 g/ l에서 간암세포(Hep3B)의 성장을 61%까지 억제하였다. 또한 혈당강하 및 혈압조절(ACE활성저해)에서도 숙지황이 건지황보다 우수한 생리활성을 나타내 최고 농도(1 g/ l )에서 각각 63.69%, 56.58%의 억제율을 보였다.

• 약학회지
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• 제46권6호
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• pp.411-415
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• 2002

We compared the hypoglycemic effects of Ginseng Radix Alba (GRA) and Mori Folium (MF) in multiple low dose (MLD) streptozotocin(STZ)-induced diabetic rats. In order to induce hyperglycemic state 25 mg/kg of STZ was injected intraperitoneally for 5 consecutive days. SD rats were randomly divided into diabetic control and treatment groups. Treatment groups were administered with either 500 mg/kg of GRA (G500), 500 mg/kg of MF (M500), or 250 mg/kg of GRA mixed with same dose of MF (GM250) for 3 weeks. Blood glucose level and body weight were measured every 5th day. G500 and M500 both significantly reduced blood glucose levels as compared to the diabetic control group (diabetic control, 458.3$\pm$25.4 mg/dl; G500, 275.0$\pm$12.0; M500, 278.0$\pm$15.4; GM250, 324.0$\pm$18.4). While body weight in diabetic control group was decreased slightly after 3 weeks, treatment groups showed gradual increases of body weight during 3 week-period. Plasma insulin level was increased by treatment with GRA, but those levels in M500 and GM250 groups were similar to the diabetic control (normal control, 32.0$\pm$13.9 $\mu$IU/mι; diabetic control, 12.4$\pm$1.9; G500, 17.5$\pm$3.4; M500, 11.1$\pm$3.2; GM250, 10.5$\pm$t3.7). Urine glucose levels were also remarkably reduced in all treatment groups (normal control, 0.0$\pm$0.0 g/day; diabetic control, 11.4$\pm$2.5; G500, 4.9$\pm$0.2; M500, 5.7$\pm$1.6 ; GM250, 8.2$\pm$0.2). At the second and third week of the treatment, food and water intakes were determined. At the third week, food and water intakes were significantly decreased in all treatment groups. Taken together, we may conclude that GRA and MF alone may prevent or delay the development of hyperglycemia, however, synergistic hypoglycemic activity was not be seen in group treated with mixed formula of GRA and MF when compared to GRA or MF alone.

• 한국식품과학회지
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• 제36권3호
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• pp.472-477
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• 2004

한의학에서 당뇨병(소갈) 처방으로 사용되는 육미지황환의 성분 중의 하나인 목단피의 혈당 강하 효과를 조사하기 위해서 목단피를 70% 에탄올로 추출한 후 XAD-4 column으로 분획하여 인슐린 민감성을 향상시키고 ${\alpha}-glucoamylase$ 활성을 억제하는 분획층이 있는지를 조사하였다. 3T3-L1 지방세포에서 소량의 인슐린(1 ng/mL)의 존재하에서 20, 40, 60% 메탄올 분획층은 인슐린 작용을 증가시켜 포도당 흡수를 효과적으로 증가시키는 인슐린 민감성 성분이 함유되어 있었다. 또한 20, 40, 60, 80% 메탄올 분획층에는 ${\alpha}-glucoamylase$의 활성을 억제하여 말토즈와 dextrin의 ${\alpha}-glucoamylase$ 결합이 분해되는 것을 억제시키는 성분이 함유되어 있었다. In vivo에서 실험동물에게 목단피의 70% 메탄올 추출물을 투여한 후 말토즈와 dextrin로 경구부하 검사를 하였을 때 dextrin만이 투여하였을 때에 대조군인 셀룰로즈에 비해 혈당의 상승이 현저하게 낮았고 이것은 dextrin의 소화 흡수의 감소에 의한 것으로 추정된다. 앞으로 효과가 있는 분획층을 더 분리하여 인슐린 민감성 물질과 ${\alpha}-glucoamylase$의 활성을 억제시키는 유효성분을 분리하고자 한다.