Browse > Article
http://dx.doi.org/10.3746/jkfn.2003.32.6.921

Hypoglycemic Effect of the Methanol Extract of Soybean Sprout in Streptozotocin-Induced Diabetic Rats  

김정인 (인제대학교 식품생명과학부)
강민정 (인제대학교 식품생명과학부)
배세연 (인제대학교 식품생명과학부)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.32, no.6, 2003 , pp. 921-925 More about this Journal
Abstract
To control blood glucose level as close to normal is the major goal of treatment of diabetes mellitus. $\alpha$-glucosidase is the enzyme to digest dietary carbohydrate and inhibition of $\alpha$-glucosidase could suppress postprandial hyperglycemia. The methanol extract of soybean sprout was tested for the inhibitory activities against $\alpha$-glucosidase in vitro. Soybean sprout extract inhibited yeast $\alpha$-glucosidase activity by 24.5% at the concentration of 5 mg/mL. The methanol extract of soybean sprout was subsequently subjected to sequential fractionation with hexane, ethyl acetate, butanol and water. Among the fractions tested ethyl acetate-soluble fraction showed relatively strong inhibition against $\alpha$-glucosidase by 36.3% at the concentration of 5 mg/mL. Acarbose, standard $\alpha$-glucosidase inhibitor, inhibited $\alpha$-glucosidase activity by 40.1%. The ability of soybean sprout extract to lower postprandial glucose was studied in streptozotocin-induced diabetic rats. Starch solution (1 g/kg) with and without the methanol extract of soybean sprout (500 mg/kg) was administered to diabetic rats after an overnight-fast by gastric intubation. A single oral dose of soybean sprout extract inhibited the increase in blood glucose levels significantly at 60, 90, 120, 180 min (p<0.05) and decreased incremental response areas under the glycemic response curve significantly (p<0.05). These results suggest that soybean sprout might exert hypoglycemic effect by inhibiting $\alpha$-glucosidase activity.
Keywords
soybean sprout; ${\alpha}$-glucosidase; hypoglycemic effect; diabetes mellitus;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 MacLennan AH, Wilson DH, Taylor AW. 1996. Prevalence and cost of alternative medicine in Australia. Lancet 347 (9001): 569-573.   DOI   ScienceOn
2 Lau AL, Failla ML. 1984. Urinary excretion of zinc, copper and iron in the streptozotocin-diabetic rats. J Nutr 114: 224-233.
3 Jenkins DJ, Wolever TM, Taylor RH, Ghafari H, Jenkins AL, Barker H, Jenkins MJ. 1980. Rate of digestion of foods and postprandial glycaemia in normal and diabetic subjects. Br Med J 281: 14-17.   DOI
4 Wong S, O'Dea K. 1983. Importance of physical form rather than viscosity in determining the rate of starch hydrolysis in legumes. Am J Clin Nutr 37: 66-70.
5 Tattersall R. 1993. $\alpha$-glucosidase inhibition as an adjunct to the treatment of type 1 diabetes mellitus. Diabet Med 10: 658-662.
6 Watanabe J, Kawabata J, Kurihara H, Niki R. 1997. Isolation and identification of $\alpha$-glucosidase inhibitors from Tochu-cha. Biosci Biotechi Biochem 61: 177-178.   DOI   ScienceOn
7 Korea National Statistical Office. 1999. The cause of death statistics 1998. Annual Report on the Cause of Death Statistics 19.
8 Fujita H, Yamagami T, Ohshima K. 2001. Fermented soybean-derived water-soluble Touchi extract inhibits $\alpha$-glucosidase and is antiglycemix in rats and humans after single oral treatments. J Nutr 13: 1211-1213.
9 Grant G, Edwards JE, Pusztai A. 1995. $\alpha$-amylase inhibitor levels in seeds generally available in Europe. J Sci Food Agric 67: 235-238.   DOI   ScienceOn
10 Mooradian AD, Thurman JE. 1999. Drug therapy of postprandial hyperglycaemia. Drugs 57: 19-29.
11 Haller H. 1998. The clinical importance of postprandial glucose. Diabetes Res Clin Pract 40: S43-S49.   DOI   ScienceOn
12 Steiner G, Haynes F, Yoshino G. 1984. Hyperinsulinemia and in vivo very-low-density lipoprotein triglyceride kinetics. Am J Physio 246: 187-192.
13 Forman LJ, Estilow S, Lewis M, Vasilenko P. 1986. Streptozotocin diabetes alters immunoreactive beta-endorphin levels and pain perception after 8 wk in female rats. Diabetes 35: 1309-1313.   DOI   ScienceOn
14 Fujita H, Yamagami T, Ohshima K. 2003. Long-term ingestion of Touchi extract, $\alpha$-glucosidase inhibitor, by borderline and mild type-2 diabetic subjects is safe and significantly reduces blood glucose levels. Nutr Res 23: 713-722.   DOI   ScienceOn
15 Nishioka T, Kawabata J, Aoyama Y. 1998. Baicalein, an alpha-glucosidase inhibitor from Scutellaria baicalensis. J Nat Prod 61: 1413-1415.   DOI   ScienceOn
16 Yoshiaki Y. 1988. Inhibition of intestinal $\alpha$-glucosidase and postprandial hyperglycemia by Moranoline and its N-alkyl derivatives. Agric Biol Chem 52: 121-128.   DOI
17 Young IR, Stout RW. 1987. Effects of insulin and glucose on the cells of the arterial wall: Interaction of insulin with dibutyryl cyclic AMP and low density lipoprotein in arterial cells. Diabetes Metab 13: 301-306.
18 Korea National Statistical Office. 2002. The cause of death statistics 2001. Annual Report on the Cause of Death Statistics 21.
19 Yu HJ, Song OG. 1985. Dietary therapy for diabetes mellitus. Diabetes Mellitus 9: 21-25.
20 Koivisto VA. 1993. Insulin therapy in type II diabetes. Diabetes Care 16: 29-39.   DOI
21 Jenkins DJ, Wolever TM, Jenkins AL. 1988. Starchy foods and glycemic index. Diabetes Care 11: 149-159.   DOI   ScienceOn
22 Kim SH, Yang JL, Song YS. 1999. Physiological functions of Chongkukjang. Food Industry and Nutr 4: 40-46.   과학기술학회마을
23 Asano N, Oseki K, Tomioka E, Kizu H, Matsui K. 1994. N-containing sugars from Morus alba and their glycosidase inhibitory activities. Carbohydr Res 259: 243-255.   DOI   ScienceOn
24 Kim JS, Kwon CS, Son KH. 2000. Alpha-glucosidase inhibitory activities of some wild vegetable extracts. J Food Sci Nutr 5: 174-176.   과학기술학회마을
25 Asano N, Tomioka E, Kizu H, Matsui K. 1994. Sugars with nitrogen in the ring isolated from the leaves of Morus bombycis. Carbohydr Res 253: 235-245.   DOI   ScienceOn
26 Goldmann A, Milat ML, Ducrot PH. 1990. Tropane derivatives from Calystegia sepium. Phytochemistry 29: 2125-2128.   DOI   ScienceOn
27 Park SH, Lee HS. 1999. Effects of legume supplementation on the gastrointestinal function and diabetic symptoms in streptozotocin-induced diabetic rats. Korean J Nutr 32: 617-627.   과학기술학회마을
28 Madar Z. 1984. Effect of brown rice and soybean dietary fiber on the control of glucose and lipid metabolism in rats. Am J Clin Nutr 43: 388-396.
29 Defronzo RA. 1981. The effect of insulin on renal sodium metabolism. Diabetologia 21: 165-171.
30 Lebovitz HE. 1998. Postprandial hyperglycemic state: importance and consequences. Diabetes Res Clin Pract 40: S27-S28.   DOI   ScienceOn
31 Fujita H, Yamagami T. 2001. Fermented soybean-derived Touchi extract with anti-diabetic effect via $\alpha$-glucosidase inhibitory action in a long-term administration study with $KKA^y$ mice. Life Science 70: 219-227.   DOI   ScienceOn
32 Fujita H, Yamagami T, Ohshima K. 2001. Efficacy and safety of Touchi extract, $\alpha$-glucosidase inhibitor derived from fermented soybeans, in non-insulin-dependent diabetic mellitus. J Nutr Biochem 12: 351-356.   DOI   ScienceOn
33 Elbein AD, Mitchell M, Sanford BA, Fellows LE, Evans SV. 1984. The pyrrolidine alkaloid, 2,5-dihydroxymethyl-3-4-dihydroxypyrrolidine, inhibit glycoprotein processing. J Biol Chem 259: 12409-12413.
34 Hanefeld M. 1998. The role of acarbose in the treatment of non-insulin-dependent diabetes mellitus. J Diabetes Complications 12: 228-237.   DOI   ScienceOn
35 Baron AD. 1998. Postprandial hyperglycemia and $\alpha$-glucosidase inhibitors. Diabetes Res Clin Pract 40: S54-S55.
36 Heo GB. 1985. Exercise therapy for diabetes mellitus. Diabetes Mellitus 9: 5-10.
37 Inoue I, Takahashi K, Noji S, Awata T, Negishi K, Katayama S. 1997. Acarbose controls postprandial hyper-proinsulinemia in non-insulin-dependent diabetes mellitus. Diabetes Res Clin Prac 36: 143-151.   DOI   ScienceOn