• 한국약용작물학회지
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• 제21권4호
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• pp.255-261
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• 2013

The purpose of this study was to examined the antioxidant activities by water and 70% ethanol extract from durian (Durio zibethinus.) seed, sarcocarp and peel. Durian extract were studied for reducing sugar content, polyphenol content, superoxide dismutase (SOD) like activity, electron donating ability, nitrite scavenging ability, flavonoid content, hydroxy radical scavenging activity. Reducing sugar content were increased peel > sarcocarp > seed. Total polyphenol, flavonoid content, DPPH radical scavenging ability and SOD like activity were increased seed > peel > sarcocarp. Total polyphenol content was relatively high as $21.90{\pm}0.50mg/g$ in the ethanol extract of the seed. DPPH radical scavenging ability was relatively high as $62.08{\pm}2.63%$ in the water extract of the seed. Nitrite scavenging ability was no significant difference. Hydroxy radical scavenging activity was increased seed > peel > sarcocarp, was relatively high as $58.27{\pm}1.13%$ in the water extract of the seed.

• 한국식품과학회지
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• 제35권5호
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• pp.988-993
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• 2003

대파에 함유된 항산화 물질을 구명하고자 대파를 3가지 다른 방법으로 추출하여 대파 착즙액, 열수추출물, MeOH 추출물을 얻었다. 이들 추출물을 각각 용매분획하여 얻어진 EtOAc 가용 산성획분을 silica gel adsorption column chromatography로 정제하여 얻어진 활성획분을 GC-MS 분석하였다. 그 결과, 대파 착즙액과 열수추출물에서는 succinic acid ,fumaric acid, 4-hydroxybenzoic acid, 4-hydroxy-3-methoxy-benzoic acid, 4-hydroxycinnamic acid 그리고 4-hydroxy-3-methoxycinnamic acid의 6종 화합물이 동정되었으며, MeOH 추출물로부터는 4-hydroxybenzoic acid, 4-hydroxy-3-methoxy-benzoic acid, 4-hydroxycinnamic acid, 4-hydroxy-3-methoxy-cinnamic acid의 4종 화합물이 동정되었다. 동정된 이들 화합물을 대상으로 DPPH radical-scavenging 활성을 검정한 결과, 4-hydroxy-3-methoxycinnamic acid가 가장 강한 항산화 활성을 나타냈다. 또한 각각의 대파추출물에 함유된 항산화 물질의 함량을 GC에 의해 정량분석한 결과, 대파 착즙액과 열수추출물 및 MeOH 추출물 모두 강한 항산화 활성을 나타내는 4-hydroxy-3-methoxycinnamic acid가 가장 많이 함유되어 있었다.

• 한국식품영양과학회지
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• 제38권4호
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• pp.462-469
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• 2009

제주재래종 감귤류 10종에 대해서 수확시기별 총 폴리페놀 함량과 활성산소종 소거활성 변화를 조사하였다. 총 폴리페놀 함량은 지각과 홍귤이 미숙과인 8월 하순과 9월 하순에 200 mg% 내외로 가장 높았고 대부분의 감귤종이 수확시기가 늦어질수록 감소하는 경향을 보였다. 수확시기별 감귤과피의 superoxide anion radical 소거활성은 홍귤과 지각에서 60% 이상으로 가장 높았고 사두감이 가장 낮은 활성을 보였으며 홍귤과 지각을 제외한 감귤류의 수확시기별 차이는 크지 않았다. Hydrogen peroxide 소거활성은 8월 하순에 사두감이 73.8%로 가장 높은 소거활성을 나타내었고 그 다음에 지각이 거의 70%의 소거활성을 보였으며, 대부분의 감귤종이 수확시기가 늦어질수록 감소하는 경향을 보였다. Hydroxy radical 소거활성은 대부분의 재래감귤종의 과피에서 비교적 높은 활성을 보였으며 지각과 당유자가 8월 하순에 각각 75.1%와 74.6%로 가장 높은 소거활성을 보였고, 그 외 감귤종도 60% 이상의 소거능을 보였으며 수확시기별 차이는 거의 없었다. 감귤이 성숙됨에 따라 hydrogen peroxide 소거능, superoxide anion 라디칼 소거능은 감소하는 경향을 보이나 nitric oxide 라디칼 소거활성은 증가하는 추세를 보였고, 병귤이 58.4%로 가장 활성이 높았으며 다른 활성산소종에서 활성이 높았던 사두감, 지각, 당유자가 활성이 가장 낮았다. 본 실험결과 제주재래종 감귤 중 특히 지각이 대부분의 활성산소종에 높은 소거활성을 보였으며, 이에 대한 구체적인 연구가 진행될 필요성이 있을 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권2호
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• pp.255-258
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• 2005

Several commercial yogurt starter cultures (L. acidophilus LA5, L. casei 01, L. acidophilus LA100, L. bulgaricus LB207 and L. rhamnosus GG744) were investigated for antioxidant activity by using in vitro assays. From the results of the present work, all strains tested showed varying degrees of antioxidant activity. L. bulgaricus LB207 showed the highest antioxidant activity, with a linoleic acid peroxidation inhibition of 81.3%. Hydroxy radical scavenging activity, ferrous iron chelating activity, reducing power and superoxide dismutase (SOD) activity were also studied. L. bulgaricus LB207 showed the highest hydroxy radical scavenging activity and L. casei 01 showed the highest chelating activity. L. bulgaricus LB207 and L. acidophilus LA100 showed good reducing power. All the strains in this study showed low SOD activity. The results of the present work suggest that antioxidant activity of L. bulgaricus LB207 due to its strong hydroxy radical scavenging activity and reducing power.

• 대한약침학회지
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• 제12권2호
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• pp.7-12
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• 2009

Objectives : The objective of this study was to compare the antioxidant effects among cultivated wild ginseng and ginseng extracts. Methods : In vitro antioxidant activities were examined by superoxide and hydroxyl radical scavenging activities of ginseng and cultivated wild ginseng extracts. Results : 1. In the superoxide radical scavenging activities of ginseng and cultivated wild ginseng extracts, antioxidant activities of cultivated wild ginseng extracts was showed higher than cultivated ginseng in the concentration of 0.25 and $0.50mg/m{\ell}$. 2. In the hydroxyl radical scavenging activities of ginseng and cultivated wild ginseng extracts, antioxidant activities of cultivated wild ginseng extracts was showed higher than cultivated ginseng in the concentration of 1.0, 2.5, and $5.0mg/m{\ell}$. Conclusions : In summary, the results of this study demonstrate that cultivated wild ginseng extracts had higher antioxidant activities to cultivated ginseng.

• Natural Product Sciences
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• 제6권3호
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• pp.135-138
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• 2000

MeOH extract of Cuscuta chinensis seeds was fractionated with n-hexane, EtOAc and BuOH successively, and antioxidant activities were tested for all fractions using DPPH free radical scavenging method. In the tested fractions, EtOAc fraction showed high antioxidant activity$(EC_{50},\;50\;{\mu}g)$ From the EtOAc fraction, five compounds have been isolated. On the basis of spectral data, these compounds were identified as ${\beta}-sitosterol$, methyl 4-hydroxy-3,5-dimethoxycinnamate, ${\beta}-sitosterol-3-O-{\beta}-D-glucopyranoside$, caffeic acid, quercetin, kaempferol and calycopteretin. Among these compounds, ${\beta}-sitosterol$ and ${\beta}-sitosterol-3-O-{\beta}-D-glucopyranoside$ showed no antioxidant activity. $EC_{50}$ values of methyl 4-hydroxy-3,5-dimethoxycinnamate, caffeic acid, quercetin, kaempferol and calycopteretin were 0.6, 8, 19, 17 and $12\;{\mu}g$, respectively.

• Archives of Pharmacal Research
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• 제28권9호
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• pp.1023-1026
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• 2005

A new furofuran lignan (1) was isolated from the n-butanol fraction of the methanolic extract of the leaves of Petasites japonicus (Sieb. et Zucc.) Maxim. (Compositae). The structure of compound 1 was determined to be $2{\alpha}-(4'-hydroxy-3'-methoxyphenyl)-6{\alpha}-(4"-hydroxy-3"-methox­yphenyl)-8{\alpha}-hydroxy-3, 7-dioxabicyclo[3.3.0]octane\;4'-O-({\beta}-D-glucopyranoside)$ by spectroscopic methods including 2D-NMR. In further studies, it was found that the compound 1 expressed an antioxidant activity in DPPH radical scavenging assay, and moreover, ameliorated the seizure in kainic acid-treated mice.

• 생명과학회지
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• 제27권10호
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• pp.1121-1129
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• 2017

4종의 식용 조류, 참김(Porphyra tenera), 미역(Undaria pinnatifida), 톳(Sargassum fusiforme), 잎파래(Enteromorpha linza)의 에탄올 추출물에 대해 ABTS+ 소거활성, 하이드록시 라디칼(OH), 산화질소(NO), 철 이온 환원력(FRAP)을 조사하였다. ABTS+ 소거활성은 Brand-Williams 등의 방법에 따랐다. 톳의 ABTS+ 소거활성은 8.0 mg/ml 일 때 61.8%로 가장 높은 반면 잎파래는 동일 농도에서 35.7%로 가장 낮았다. 참 김과 미역은 ABTS+에 대해 유사한 활성 저해를 나타내었다. 하이드록시 라디칼의 저해활성은 톳 > 참김 > 미역 > 잎파래 순이었다. 산화질소는 8.0 mg/ml 추출물일 때 톳 > 참김 > 미역 > 잎파래 순이었다. 3.5% 소금과 4종의 조류 추출물을 첨가한 밀가루 반죽을 국수틀에 뽑아낸 후 5분간 끓였을 때 전반적으로 4개의 산화제에 대해 활성저해가 저하되었다. 톳 8.0 mg/ml를 첨가한 국수의 경우 산화질소 활성저해는 27.2%였다. 미역 8.0 mg/ml을 첨가한 국수는 철 이온 환원력이 31.5%였다. 종합적으로 톳 추출물이 ABTS+, OH, NO, FRAP에 대해 활성저해가 가장 높았다. 본 연구 결과 자연적으로 분포하는 참김, 미역, 톳, 파래가 국수의 항산화력 증진에 도움을 준다고 사료되었다.

• Biomolecules & Therapeutics
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• 제13권3호
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• pp.150-155
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• 2005

The ethanolic extract of chestnut (Castanea crenata S. et Z., Fagaceae) inner shell (CISE) and one of its components, ellagic acid (EA), were evaluated for their protective effects against 1, 1-diphenyl-2-picryl hydrazine (DPPH) free radical generation and hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line. CISE and EA were shown to possess the free radical scavenging effect against DPPH radical generation, significantly. They were also found to strongly inhibit hydrogen peroxide-induced DNA damage from Chinese hamster lung (CHL) cell, assessed by single cell gel electrophoresis assay and 8-hydroxy -2'-deoxy guanosine (8-OH-2'dG) assay. Furthermore, topical application of CISE [$12.5\%$(w/w) cream] and ellagic acid [$1.0\%$(w/w) cream] for 14 days potently inhibited malondialdehyde (MDA) formation of mouse dorsal skin (a marker of lipid peroxidation) induced by ultraviolet B exposure. Therefore, CISE and its component, ellagic acid, may be the useful natural antioxidants by scavenging free radicals, inhibition of lipid peroxidation and protecting oxidative DNA damage when topically applied.

• 한국미생물·생명공학회지
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• 제45권3호
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• pp.200-208
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• 2017

Actinobacteria are prolific producers of a large number of natural products with diverse biological activities. In the present study, an actinobacterium isolated from sunflower rhizosphere soil sample collected from Medak, Andhra Pradesh, South India was identified as Amycolatopsis thermoflava strain SFMA-103. A pigmented secondary metabolite in culture broth was extracted by using methanol and it was further purified by silica gel column chromatography with methanol-chloroform solvent system. Structural elucidation studies based on UV-visible, 1D and 2D-NMR, FT-IR, and mass spectroscopic analyses confirmed the structure as 1-methoxy-3-methyl-8-hydroxy-anthraquinone. It showed significant in vitro anticancer activity against lung cancer and lymphoblastic leukemia cells with $IC_{50}$ values of 10.3 and $16.98{\mu}M$, respectively. In addition, 1-methoxy-3-methyl-8-hydroxy-anthraquinone showed good free radical scavenging activity by DPPH method with an $EC_{50}$ of $18.2{\mu}g/ml$. It also showed other promising superoxide radical scavenging, nitric oxide radical scavenging and inhibition of lipid peroxidation activities. This is a first report of anti-proliferative and antioxidant activities of 1-methoxy-3-methyl-8-hydroxy-anthraquinone isolated from A. thermoflava strain SFMA-103 which may find potential application in biotechnological and pharmaceutical fields.