• 제목/요약/키워드: hydroxy fatty acid

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Phytosterylferulate가 米糠油 暗色化에 미치는 影響에 關한 硏究 (The Darkening Effect of Phytosterylferulate on the Rice Bran Oil)

  • 김점식
    • 대한화학회지
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    • 제13권4호
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    • pp.325-332
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    • 1969
  • An attempt has been made to investigate the nature of the characteristic darkening reaction occurring in the rice bran oil during the storage. Several pigments were separated by using column and thin layer chromatography of the pigments was made in the light of the knowledge of absorption spectral behavior. The presence of trace amount of iron and phytosteryl esters of ferulic acid (3-methoxy-4-hydroxy-cinnamic acid) was found to be responsible for the development of the characteristic color in rice bran oil. The model reactions with the ferulate, iron and fatty acids could be suggested the mechanism.

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불포화 지방산과 마그네슘, 칼슘과의 상호작용이 3-Hydroxy-3-Methylglutaryl Co-A reductase 활성에 미치는 영향 (Interaction of dietary Mg(II), Ca(II) and polyunsaturated fatty acids on 3-Hydroxy-3-Methylalutaryl Co-A reductase activity)

  • 남현근
    • 한국응용과학기술학회지
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    • 제1권1호
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    • pp.3-9
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    • 1984
  • Interaction of dietary Magnesium, Calcium and Polyunsaturated fatty acid(vegetable oils)on 3-Hydroxy-3-Methylglutaryl Co-A reductase activity was studied for a period of 30 days using isocalories and isonitrogenous as a basal diet . The subject rabbits were divided into 18 feeding groups. The results are summarized as follows: 1. The ratio of ${\alpha}_1-lipoprotein$ par lipalbumin is 0.34 for control group, 0.38 the highest group fed 0.1 Mg(II) 10ml plus perilla oil and basal diet, the lowest 0.25 group fed 0.1M Mg(II) 5ml plus sesame oil and basal diet. 2. The ratio ratio of ${\alpha}_2-lipoprotein$ per lipalbumin is 0.64 for control group. 0.95 as the highest for the group fed 0.1M Ca(II) 15ml plus sesame oil and basal diet, 0.1M Ca(II) 5ml plus perilla oil and basal diet. 3. The ratio of ${\beta}-lipoprotein$ per lipalbumin is 0.71 for control group, the highest 0.81 for the groups fed 0.1M Mg(II) 10ml plus sesame oil and basal diet, the lowest 0.37 for the group fed 0.1M Mg(II) 15ml plus soybean oil and basal diet. 4. In serum triglyceride, control group was 129.5mg%, the highest 155.4mg% for the group fed 0.1M Ca(II) 5ml plus sesame oil and basal diet, the lowest 85.7mg% for the group fed 0.1M Mg(II) 10ml plus soybean oil and basal diet. 5. In serum cholesterol, control group was 96.7mg%, the highest 152.5mg% for the group fed 0.1M Ca(II) 10ml plus sesame oil and basal diet, the lowest 80.5mg% for the group fed 0.1M Mg(II) 15ml plus soybean oil and basal diet. 6. In case of HMG-CoA reductase activity, control group was 0.95, the highest 0.98 for the group fed 0.1M Ca(II) plus soybean oil and basal diet. 7. Interaction between metal(II) ions and polyunsaturated fatty acid(vegetable oil) are soybean oil>sesame oil>perilla oil, for Mg(II). soybean oil>perilla oil>sesame oil, for Ca(II). Therefore, it is invetigated that the interaction between metal ion and polyunsaturated fatty acid is the higher, the cholesterol level is the lower, and HMG-CoA reductase activity is increased.

Effects of Supplemental Liquid DL-methionine Hydroxy Analog Free Acid in Diet on Growth Performance and Gastrointestinal Functions of Piglets

  • Kaewtapee, C.;Krutthai, N.;Bunchasak, C.
    • Asian-Australasian Journal of Animal Sciences
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    • 제29권8호
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    • pp.1166-1172
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    • 2016
  • This study was conducted to determine the effect of dietary supplementation of liquid DL-methionine hydroxy analog free acid (DL-MHA) on growth performance and gastrointestinal conditions of piglets. One hundred and eighty crossbred barrow piglets (Large White${\times}$Landrace, body weight: $12.48{\pm}0.33kg$) were divided into three groups with ten replications of six piglets each. Piglets received DL-MHA in diet at a concentration of 0 (control group), 0.15%, or 0.24%. The results indicated that increasing the standardized ileal digestible (SID) of sulfur amino acids (SAA) to lysine (SID SAA:Lys) ratio by supplementation of DL-MHA tended to increase (quadratic; p<0.10) weight gain and ADG, and showed slightly greater (linear; p<0.10) gain:feed ratio. The pH in the diet and cecum linearly decreased (p<0.01), whereas pH in colon had a quadratic response (p<0.01) with increasing supplementation of DL-MHA. By greater supplementation of DL-MHA, the population of Lactobacillus spp. in rectum was likely to increase (quadratic; p<0.10), but Escherichia coli population in the diet was reduced (quadratic; p<0.05). Acetic acid concentration and total short-chain fatty acids in cecum linearly increased (p<0.05), whereas valeric acid in cecum quadratically increased (p<0.05) with increasing DL-MHA levels. Moreover, the villous height of the jejunum quadratically increased (p<0.01) as the supplementation of DL-MHA was increased. It is concluded that the addition of DL-MHA in diet improved the growth performance and the morphology of gastrointestinal tract of piglets.

Endophytic Bacillus sp. CY22 from a Balloon Flower (Platycodon grandiflorum) Produces Surfactin Isoforms

  • Cho, Soo-Jeong;Hong, Su-Young;Kim, Jin-Young;Park, Sang-Ryeol;Kim, Min-Keun;Lim, Woo-Jin;Shin, Eun-Chule;Kim, Eun-Ju;Cho, Yong-Un;Yun, Han-Dae
    • Journal of Microbiology and Biotechnology
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    • 제13권6호
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    • pp.859-865
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    • 2003
  • Surfactin is a mixture of cyclic lipopeptides built from variants of a heptapeptide and a ${\beta}-hydroxy$ fatty acid produced by several strains of Bacillus sp. Surfactin isoforms produced by endophytic Bacillus sp. CY22 from a balloon flower were isolated and characterized. It was found that the purified surfactin had three isoforms with protonated masses of m/z 1,008, 1,022, and 1,036, and different structures in combination with Na, K, Ca ions using MALDI-TOF MS, ESI-MS/MS, and ICP MS, respectively. In the MS/MS analysis, the isolated surfactin had the identical amino acid sequence (LLVDLL) and hydroxy fatty acids (with 13 to 15 carbons in length), even though isolated from different Bacillus strains. The sfp22 gene, required for producing the surfactin, consisted of an open reading frame (ORF) of 675 bp encoding 224 amino acid residues with a signal peptide of 20 amino acids. The predicted amino acid sequence of sfp22 was very similar to that of Ipa-8.

Integrative Omics Reveals Metabolic and Transcriptomic Alteration of Nonalcoholic Fatty Liver Disease in Catalase Knockout Mice

  • Na, Jinhyuk;Choi, Soo An;Khan, Adnan;Huh, Joo Young;Piao, Lingjuan;Hwang, Inah;Ha, Hunjoo;Park, Youngja H
    • Biomolecules & Therapeutics
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    • 제27권2호
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    • pp.134-144
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    • 2019
  • The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased with the incidence of obesity; however, the underlying mechanisms are unknown. In this study, high-resolution metabolomics (HRM) along with transcriptomics were applied on animal models to draw a mechanistic insight of NAFLD. Wild type (WT) and catalase knockout (CKO) mice were fed with normal fat diet (NFD) or high fat diet (HFD) to identify the changes in metabolic and transcriptomic profiles caused by catalase gene deletion in correspondence with HFD. Integrated omics analysis revealed that cholic acid and $3{\beta}$, $7{\alpha}$-dihydroxy-5-cholestenoate along with cyp7b1 gene involved in primary bile acid biosynthesis were strongly affected by HFD. The analysis also showed that CKO significantly changed all-trans-5,6-epoxy-retinoic acid or all-trans-4-hydroxy-retinoic acid and all-trans-4-oxo-retinoic acid along with cyp3a41b gene in retinol metabolism, and ${\alpha}/{\gamma}$-linolenic acid, eicosapentaenoic acid and thromboxane A2 along with ptgs1 and tbxas1 genes in linolenic acid metabolism. Our results suggest that dysregulated primary bile acid biosynthesis may contribute to liver steatohepatitis, while up-regulated retinol metabolism and linolenic acid metabolism may have contributed to oxidative stress and inflammatory phenomena in our NAFLD model created using CKO mice fed with HFD.

BHT (Butylated hydroxy toluene)의 항산화성(抗酸化性)에 대한 citric acid의 상승효과(相乘效果) (Synergistic Effect of Citric Acid on Antioxidant Property of BHT)

  • 이희봉
    • Journal of Nutrition and Health
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    • 제8권2호
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    • pp.31-36
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    • 1975
  • 식용대두유(食用大豆油)에 0.02% BHT, 0.01% citric acid 및 0.1 ppm $CuSo_4\;5H_2O$를 단독 또는 혼합 첨가하여 암소저장조건하(暗所貯藏條件下)$(40{\pm}0.5^{\circ}C)$ 및 일사광선조사조건하(日射光線照射條件下)에서 저장(貯藏)하여 과산화물가(過酸化物價)와 유리지방산가(遊離脂肪酸價)의 변화(變化)를 측정하여 다음과 같은 결과(結果)를 얻었다. 1. 일반적(一般的)으로 일사광선조사조건(日射光線照射條件)에서는 암소저장조건(暗所貯藏條件)에서 보다 과산화물가(過酸化物價)와 유리지방산가(遊離脂肪酸價)가 빠른 속도로 상승하였다. 2. $CuSO_4{\cdot}5H_2O$를 첨가한 시료구(試料區)에서는 일사광선조건(日射光線條件)과 암소저허조건(暗所貯虛條件)에서 과산화물(過酸化物)과 유리지방산(遊離脂肪酸)을 강(强)하게 형성(形成)하였다. 3. BHT에 대한 citric acid의 상승효과는 암소저장조건(暗所貯藏條件)과 일사광선조건(日射光線條件)에서 모두 강(强)하게 나타났다.

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수영만의 해양세균의 분포와 우점속의 균체 지방산 조성 (Distribution of Marine Bacteria and Celluar Fatty Acid Composition of Dominated Genus in Suyeong Bay)

  • 강원배;성희경;문창호;이원재
    • 한국수산과학회지
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    • 제30권4호
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    • pp.640-651
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    • 1997
  • 수영만에서 서식하고 있는 해양세균의 분포 및 생태학적 특성을 조사하기 위하여 수영만에서 해양세균의 시${\cdot}$공간적 분포 양상의 변화 및 조사기간동안 수영만에서 분리된 해양세균중 우점속의 균체 지방산 조성을 조사하였다. 조사기간동안 연구 해역의 총균수와 생균수의 분포는 각각 $10^7\~10^8/m\ell$$10^4\~10^6\;cfu/m\ell$ 범위였으며, 총균수는 표층과 저층간에 큰 차이를 보이지 않았지만, 생균수는 저층의 경우 동계에서 하계로 갈수록 점차 감소하는 경향을 보였다. 또한 해양세균 총 303균주가 분리 동정되었으며, 그 중 Pseudomonas spp. $(30.4\%)$가 가장 많이 출현하였고, 그 다음으로 Acinetobacter spp. $(18.8\%)$, Vibrio spp. $(13.2\%)$, Flavobacterium spp. $(12.2\%)$, Bacillus spp. $(8.9\%)$ 등이 우점하였다. 수영만에서 분리된 해양세균 중 Gram 음성세균 중에서 가장 우점한 Pseudomonas속과 Gram 양성 세균중에서 가장 우점한 Baillus속 각각 5균주의 균체 지방산 조성을 조사한 결과 검출된 균체 지방산은 총 31종이었으며, Pseudomonas속에 비해 Bacillus속의 지방산 형태는 비교적 단순했다. 또한 Pseudomonas속의 균체 지방산에서는 단순불포화지방산이 높은 비율로 검출되었고 Bacillus속 에서는 쇄상지방산이 우점한 지방산 형태였다. 또한 Pseudomonas속에서 검출된 OHFA와 CFA는 Baillus속의 균체 지방산 조성에서는 전혀 검출되지 않았다. Pseudomonas속의 균체 지방산은$C_{16:1},\;C_{17:1},\;C_{18:1}\;and\;C_{16:0}$이 우점했고, Baillus속 세균들에서는 $C_{15:0}\;iso\;,\;C_{15:0}\;anteiso,\;C_{17:0}\;iso\;,C_{17:0}$가 많이 검출되었다.

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Effects of Aspergillus Oryzae Culture and 2-Hydroxy-4-(Methylthio)-Butanoic Acid on In vitro Rumen Fermentation and Microbial Populations between Different Roughage Sources

  • Sun, H.;Wu, Y.M.;Wang, Y.M.;Liu, J.X.;Myung, K.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권9호
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    • pp.1285-1292
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    • 2014
  • An in vitro experiment was conducted to evaluate the effects of Aspergillus oryzae culture (AOC) and 2-hydroxy-4-(methylthio)-butanoic acid (HMB) on rumen fermentation and microbial populations between different roughage sources. Two roughage sources (Chinese wild rye [CWR] vs corn silage [CS]) were assigned in a $2{\times}3$ factorial arrangement with HMB (0 or 15 mg) and AOC (0, 3, or 6 mg). Gas production (GP), microbial protein (MCP) and total volatile fatty acid (VFA) were increased in response to addition of HMB and AOC (p<0.01) for the two roughages. The HMB and AOC showed inconsistent effects on ammonia-N with different substrates. For CWR, neither HMB nor AOC had significant effect on molar proportion of individual VFA. For CS, acetate was increased (p = 0.02) and butyrate was decreased (p<0.01) by adding HMB and AOC. Increase of propionate was only occurred with AOC (p<0.01). Populations of protozoa ($p{\leq}0.03$) and fungi ($p{\leq}0.02$) of CWR were differently influenced by HMB and AOC. Percentages of F. succinogenes, R. albus, and R. flavefaciens (p<0.01) increased when AOC was added to CWR. For CS, HMB decreased the protozoa population (p = 0.01) and increased the populations of F. succinogenes and R. albus ($p{\leq}0.03$). Populations of fungi, F. succinogenes (p = 0.02) and R. flavefacien (p = 0.03) were increased by adding AOC. The HMB${\times}$AOC interactions were noted in MCP, fungi and R. flavefacien for CWR and GP, ammonia-N, MCP, total VFA, propionate, acetate/propionate (A/P) and R. albus for CS. It is inferred that addition of HMB and AOC could influence rumen fermentation of forages by increasing the number of rumen microbes.

아조벤젠을 함유한 장쇄 지방산 단분자 막의 광이성화 현상에 관한 연구 (A Study on the Photoisomerization of Monolayer Film of Long Chain Fatty Acids Containing Azobenzene)

  • 김무군;박태곤;박근호
    • 한국응용과학기술학회지
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    • 제13권1호
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    • pp.75-85
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    • 1996
  • The absorption spectra of synthesis of long chain fatty acid containing azobenzene start from p-(p'-hydroxy phenyl azo)-benzoic acid was investigated by ultraviolet spectrophotometery in chloroform solvent at the various temperature. In addition, The pressure-area of the water-air interface was obtained and the LB film was fabricated onto a quartz slide and quartz crystal by conventional Langmuir-Blodgett(LB) method. The UV absorption spectra of Langmuir-Blodgett(LB) film on quartz slide and spectrum of monolayer formed on quartz crystal have been measured. Long chain fatty acid containing azobenzene are induced phtoisomerization by the application of u. v. and visible light irradiation alternatively the reversibility of phtoisomerization was more clear difference when the number of $C_{n}$ increased but, not so good at $C_{14}-azo$. At the pressure-area isotherms, the value of surface pressure increment were decreased when the number of $C_{n}$ increased. A surface pressure of 20mN/m was obtained as a proper one for a film deposition. The photoisomerization at LB films were induced by application of UV and visible light irradiation alternatively. So the LB film of long chain fatty acid containing azobenzene has possibility to being applied to functional molecular devices such as photomemory and light switching.

기체크로마토그래피법에 의한 타액내 유기산의 신속한 스크리닝 (Rapid Gas Chromatographic Screening of Saliva Samples for Organic Acids)

  • 김경례;김정한;박영준;김정옥
    • 약학회지
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    • 제39권3호
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    • pp.283-288
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    • 1995
  • Rapid gas chromatographic profiling method was applied to saliva from healthy subjects for the analysis of free organic acids. Saliva samples were first saturated with NaHCO$_{3}$ and extracted with diethyl ether. The aqueous phase was solid-phase extracted using Chromosorb P as the adsorbent and diethyl ether as the eluent after the acidification and NaCl saturation, followed by triethylamine treatment. The resulting tiiethylammonium salts of acids were directly converted into stable tert.-butyl-dimethylsflyl derivatives, with subsequent analysis by dual-capiuary column gas chromatography and gas chromatograpy -mass spectrometry. From the ten saliva samples studied, twenty eight free organic acids including various fatty acids, hydroxy acids, dicarboxylic acids, md aromatic acids were tentatively identified. Among the acids identified , the concentration of lactic acid was highest for five saliva samples while $\alpha$-hydroxyisocaproic acid was most abundant for me sample, and succinic acid and glycolic acid for two samples. respectively. When the GC profiles were simplified to the corresponding acid retention index spectra of bar graphical form, they presented characteristic patterns for each individual.

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