• Korean Journal of Microbiology
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• v.45 no.2
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• pp.140-147
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• 2009

In this study, anaerobic enrichment cultivation was performed with the sediments from the Gimpo and Inchon areas. Lactate as an electron donor and PCE as an electron acceptor was injected into the serum bottle with an anaerobic medium. After the incubation of 8 weeks, the reductive dechlorination of PCE was observed in 7 sites among 16 sites (43%). Three enrichment cultures showed completely dechlorination of PCE to ethene, while four enrichment culture showed transformation of PCE to cis-DCE. The bacterial community structure was analyzed by PCR-DGGE. Dechlorinating bacteria were detected by species-specific primers. The dominant species in seven anaerobic enrichments were found to belong to the genus of Dehalococcoides sp. and Geobacter sp., and Dehalobacter sp.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.11
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• pp.5448-5454
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• 2013

The purpose of this study is to provide preliminary data to improve the quality of vocational education program by analyzing basic occupational skills and acquired knowledge of vocational college graduates. A total of 236 graduates were sampled to examine their competency importance level - competency performance level, and study differences between competency importance level and contribution level of college education. The paired sample t-test and importance-performance analysis were employed to investigate meaningful differences between competency importance level and Competency performance level in regard to acquired knowledge and basic occupational skills. Meaningful differences were found in 12 areas at significance level of 0.01. According to importance-performance analysis, differences were presented in 4 areas in the first quadrant, 1 in the second quadrant, 4 in the third quadrant, and 3 in the fourth quadrant. In comparison study between competency importance level and college contribution level, the latter was found to be relatively less influential as differences were presented in all of 12 areas at significance level of 0.01. This research will helps to enhance the practicality and effectiveness of education program in vocational college.

• Journal of Pharmaceutical Investigation
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• v.38 no.1
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• pp.15-21
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• 2008

Liposomes as one of the efficient drug carriers have some shortcomings such as their short circulation time, fast clearance from human body by reticuloendothelial system (RES) and limited intracellular uptake to target cell. In this study, polyethylenglycol (PEG)-incorporated cationic liposomes were prepared by ionic complexation of positively charged liposomes with carboxylated polyethyleneglycol (mPEG-COOH). The cationic liposomes had approximately $98.6{\pm}1.0nm$ of mean particle diameter and $42.8{\pm}0.8mV$ of zeta potential value. The PEG-incorporated cationic liposomes had $110.1{\pm}1.2nm$ of mean particle diameter with an increase of about 10 nm compared to the cationic liposomes. Zeta potential value of them was $12.9{\pm}0.6mV$ indicating 30mV decrease of cationic charge compared to the cationic liposomes. The amount of PEG which was incorporated onto the cationic liposomes was assayed by using picrate assay method and the incorporation efficiency was $58.4{\pm}1.1%$. Loading efficiency of model drug, doxorubicin, into cationic liposomes or PEG-incorporated cationic liposomes was about $96.0{\pm}0.7%$. Results of intracellular uptake which were evaluated by flow cytometry analysis of doxorubicin loaded liposomes showed that intracellular uptake of PEG-incorporated cationic liposomes was higher than the cationic liposomes or DSPE-mPEG liposomes. In addition, cytotoxicity of PEG-incorporated cationic liposomes was comparable to cationic liposomes. Consequently, the PEG-incorporated cationic liposomes of which surface was incorporated with PEG by ionic complex may be applicable as anticancer drug carriers that can increase therapeutic efficacy.

• Development and Reproduction
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• v.13 no.4
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• pp.313-320
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• 2009

The aim of this study was to test if 3,5,3'-triiodothyronine (T3) are involved in the osmoregulatory actions in euryhaline starry flounder Platichthys stellatus. We investigated osmoregulatory parameters ($Na^+,\;Cl^-$ and osmolality), blood cortisol and glucose in starry flounder acclimated to seawater (SW, 33 psu) and that were transferred and allowed to acclimate to freshwater (FW, 0 psu). Fish in SW were injected with T3 (5, 10, and $15{\mu}g$/g body weight) or vehicle (0.9% NaCl), and then transferred to FW. They were sampled 3 days after the transfer. With T3 at $10{\mu}g$/g, levels of plasma $Na^+$ and $Cl^-$ were significantly higher than in sham (only saline) and control fish (without hormone and saline). Osmolality was significantly higher after injection with T3 at 10 and $15{\mu}g$/g than in the control. However, T3 at $5{\mu}g$/g had no effect on hyper-osmoregulation. In this study, all dose of T3 induced a significant increases in plasma cortisol without glucose. These results suggest a positive hyper-osmoregulatory role of T3 in starry flounder to hypoosmotic environment, maybe a positive interaction of T3 with cortisol for maintenance of hyper-osmoregulatory ability.

• Journal of Korean Society of Disaster and Security
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• v.13 no.2
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• pp.11-25
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• 2020

This study is a basic study to improve responsiveness in the event of a disaster in the landside area of the Airport Passenger Terminal, and it investigated the airport emergency plan and the status of emergency response in the event of a disaster by the airline's ground staff. The Airport Emergency Plan examined the airport evacuation plan of Korea's Gimpo Airport and Japan's Ministry of Land, Infrastructure, and Transport, confirming that the domestic airport emergency plan was not specific. In addition, a survey of 100 airline ground employees working at check-in counters found that 69% of the respondents were unaware of their role in disaster situations, 90% were not educated in emergency response and 98% were not trained in disaster training in landside areas. Cross-analysis confirmed significant results in the safety of the airport where the service life worked and whether it was a role in the event of a disaster. Therefore, it is suggested that the airport emergency response plan should be improved to improve the ability to respond to disaster sites in the landside area of the airport passenger terminal and that the airline's ground staff should be educated and trained to enhance disaster response.

• The Korean Journal of Pesticide Science
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• v.10 no.3
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• pp.189-195
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• 2006

The purpose of the current study was to develop a standard method to assess the ecological effect of pesticides using the representative Korean freshwater cladocerans. The indigenous cladocerans, Daphnia obtusa, Daphnia sp., Moina macrocopa, Simocephalus vetulus and Daphnia magna formally endorsed by the major international organizations were tested to select optimal culture media in laboratory condition. All of the Korean water flea tested showed good growth and fecundity in the moderately hard water ($CaCO_3$ ranged 80 to 100 mg $L^{-1}$) and soft water ($CaCO_3$ ranged 40 to 48 mg $L^{-1}$). However, considering that the total hardness of fresh water collected from 5 rivers ranged from 60.2 to 127.2 mg $L^{-1}$ ($CaCO_3$) and Ca deficiency could be a major determinant of species success and community structure among crustacean zooplankton, the present study suggests that the optimal water hardness for growing Daphnia sp., Daphnia obtusa, Moina macrocopa and Simocephalus vetulus is $80{\sim}100$ mg $L^{-1}$ of $CaCO_3$.

• The Korean Journal of Pesticide Science
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• v.9 no.3
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• pp.221-230
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• 2005

Temperature is an important ambient factor affecting the physiology and metabolism of aquatic invertebrates. In this study, we studied about the survival, reproduction and growth effects of 4 different temperatures(16, 20, 24, $28^{\circ}C$) in four Korean cladoceran, Daphnia sp., Daphnia obtusa, Moina macrocopa, Simocephalus vetulus and Daphnia magna as an international standard species. All 5 water flea tested showed that molting time, fecundity and intrinsic rate of natural increase(r) in high temperature condition were higher than those in lower temperature. On the other hand, lower survivals and longer time to start of offspring were showed in high temperature. Our results suggest that the optimal temperatures of Daphnia sp.,, Moina macrocopa and Simocephalus vetulus seem to be about $20^{\circ}C$ except for Daphnia obtusa which as showed good survivals and reproductions in $16^{\circ}C$.

• The Korean Journal of Pesticide Science
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• v.10 no.3
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• pp.196-200
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• 2006

In this study we investigated fecundity and survivorship of four staple Korean freshwater cladocerans for their whole life to develop a new standard method used for ecological risk assessment of pesticide. The tested Korean freshwater cladocerans were Daphnia obtusa, Daphnia sp., Moina macrocopa and Simocephalus vetulus. In addition, standard test species, Daphnia magna endorsed formally by the major international organizations was tested together in order to compare with the reproductive characteristics of Korean cladocerans. A total of 358 young was produced by D. magna, whereas, 297 young was reduced by Daphnia sp. throughout the entire life. The average life span of Daphnia magna was 50 days much longer than any other Korean species. Nevertheless all of the Korean water flea tested showed good fecundity, produced over 60 young for 21 days as the validation criterion for reproduction toxicity test in Ecological Effects Test Guidelines published by USEPA. Especially Moina macrocopa produced their first brood in 4 days. Therefore 10 day reproduction test with Moina macrocopa may be an alternative good method to save cost and time to elucidating the effects of hazardous substances on the reproduction of aquatic invertebrates.

• The Korean Journal of Pesticide Science
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• v.3 no.1
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• pp.57-65
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• 1999

The midge Chironomus riparius is a member of widespread dipteran insect which has been used for sediment toxicity test. The 2nd-3rd larvae Chironomus riparius were exposed to $^{14}C$-lambda-cyhalothrin in laboratory water only system(A) and sediment-water system(B) at $20{\pm}2^{\circ}C$. The concentration was nominally in the range of 0.16 to $20{\mu}g/{\ell}$ and 62.5 to 8000 ${\mu}g/kg$, respectively. System(A) was prepared by applying the chemical to 250 ml water and introducing Chironomus riparius larvae into the system(day 0). System(B) was prepared by applying the chemical to the 25 g of soil and 250 ml water and shaking and rolling them for two hours. After allowing the system to settle for two days, Chironomus riparius larvae were introduced into the system. After 72 hours, the two systems were terminated and dead larvae were observed. The measurement of residue in water was conducted at the start(day 0) and the end of the study(day 3). The residue of $^{14}C$-lambda-cyhalothrin in water was accounted for approximately 65% of nominal concentration in system(A) and $0.2{\sim}0.4%$ in system) on 3 days. $LC_{50}$ was less than $0.156{\mu}g/{\ell}$ in system(A) and 889 ${\mu}g/kg$ in system(B), based on the nominally applied concentration.

• Korean Security Journal
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• no.51
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• pp.173-195
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• 2017

Recently, in South Korea, security management has been strengthened, but there have been an increasing number of cases where the main infrastructure of the country is hacked in the cyber space. South Korea is equipped with sophisticated information and communication technologies, such as Internet, but is threatened by cyber terrorism of North Korea and terrorist organizations. Nevertheless, there is a limit to how to develop a policy and strategic plan for the country, which is related to domestic terrorism and lacks legal and regulatory facilities, and therefore, in this study, proposed suggestions for building adaptive and efficient policy formulation. Based on the theoretical analysis framework of the Strategic Plan for achieving the objectives of the research, we compared the UK 's security strategy with the national security policy of the domestic government. As a result, several problems were derived: First, the domestic security strategy did not take into account the external environment. Secondly, lack of coordination with domestic cyber security goals setting and strategy is causing ambiguity and confusion. Third, the detailed plan of implementation of national security in each province is designed to ensure that there is a possibility that a mixed side effect between ministries and agencies will arise. Fourth, it was found that there was a limit to prepare the evaluation standards for the evaluation and return of domestic security policies in the country. Therefore, in order to establish a policy for the response of domestic cyber terrorism, we set up a vision from long-term perspectives and concrete targets based on the strategic approach of the security policy, It is necessary to present an assignment and formulate an efficient execution plan. It is necessary to maintain and improve the domestic safeguards in order to be able to complement the problems through evaluation and feedback.