Browse > Article

Analysis of Microbial Community During the Anaerobic Dechlorination of Tetrachloroethylene (PCE) in Stream of Gimpo and Inchon Areas  

Kim, Byung-Hyuk (Environmental Bio Research Center, KRIBB)
Baek, Kyung-Hwa (Environmental Bio Research Center, KRIBB)
Cho, Dea-Hyun (Environmental Bio Research Center, KRIBB)
Sung, Youl-Boong (Research Institute of Industrial Science & Technology)
Ahn, Chi-Yong (Environmental Bio Research Center, KRIBB)
Oh, Hee-Mock (Environmental Bio Research Center, KRIBB)
Koh, Sung-Cheol (Division of Civil & Environmental Engineering, Korea Maritime University)
Kim, Hee-Sik (Environmental Bio Research Center, KRIBB)
Publication Information
Korean Journal of Microbiology / v.45, no.2, 2009 , pp. 140-147 More about this Journal
Abstract
In this study, anaerobic enrichment cultivation was performed with the sediments from the Gimpo and Inchon areas. Lactate as an electron donor and PCE as an electron acceptor was injected into the serum bottle with an anaerobic medium. After the incubation of 8 weeks, the reductive dechlorination of PCE was observed in 7 sites among 16 sites (43%). Three enrichment cultures showed completely dechlorination of PCE to ethene, while four enrichment culture showed transformation of PCE to cis-DCE. The bacterial community structure was analyzed by PCR-DGGE. Dechlorinating bacteria were detected by species-specific primers. The dominant species in seven anaerobic enrichments were found to belong to the genus of Dehalococcoides sp. and Geobacter sp., and Dehalobacter sp.
Keywords
Dehalococcoides sp.; Geobacter sp.; PCR-DGGE; reductive dechlorination;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 환경부. 2008. 2008 환경통계연감
2 Futagami, T., M. Goto, and K. Furukawa. 2008. Biochemical and genetic bases of dehalorespiration. Chem. Rec. 8, 1-12   DOI   ScienceOn
3 He, J., K.M. Ritalahti, K.L. Yang, S.S. Koenigsberg, and F.E. Loffler. 2003. Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium. Nature 424, 62-65   DOI   ScienceOn
4 Loffler, F.E., Q. Sun, J. Li, and J.M. Tiedje. 2000. 16S rRNA genebased detection of tetrachloroethene-dechlorinating Desulfuromonas and Dehalococcoides species. Appl. Environ. Microbiol. 66, 1369-1374   DOI   ScienceOn
5 Lane, D.J. 1991. 16S/23S rRNA sequencing. In E. Stackebrandt and M. Goodfellow (eds.), Nucleic acid techniques in bacterial systematics. John Wiley & Sons, Inc., New York, N.Y., USA.
6 Lee, J.W., B.H. Kim, C.Y. Ahn, H.S. Kim, B.D. Yoon, and H.M. Oh. 2005. Analysis of microbial community during the anaerobic dechlorination of perchloroethylene and trichloroethylene. Kor. J. Microbiol. 41, 281-286   과학기술학회마을   ScienceOn
7 Paster, B.J., F.E. Dewhirst, I. Olsen, and G.J. Fraser. 1994. Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria. J. Bacteriol. 176, 725-732   DOI   PUBMED   ScienceOn
8 Sung, Y., K.M. Ritalahti, R.P. Apkarian, and F.E. Loffler. 2006. Quantitative PCR confirms purity of strain GT, a novel trichloroethene-to-ethene-respiring Dehalococcoides isolate. Appl. Environ. Microbiol. 72, 1980-1987   DOI   ScienceOn
9 Wild, A., R. Hermann, and T. Leisinger. 1996. Isolation of an anaerobic bacterium which reductively dechlorinates tetrachloroethene and trichloroethene. Biodegradation 7, 507-511   DOI   ScienceOn
10 문부영, 이태호, 박태주. 2004. PCR-DGGE법을 이용한 혐기성 PCE 탈염소화 배양액의 미생물 군집해석. 대한환경공학회 2004 춘계학술연구발표회 논문집 C-4-3
11 Duhamel, M. and E.A. Edwards. 2006. Microbial composition of chlorinated ethene-degrading cultures dominated by Dehalococcoides. FEMS Microbiol. Ecol. 58, 538-549   DOI   ScienceOn
12 Sung, Y., K. Ritalahti, R. Sanford, J. Urbance, S. Flynn, J.M. Tiedje, and F.E. Loffler. 2003. Characterization of two tetrachloroethene-reducing, acetate-oxdizing anaerobic bacteria and their description as Desulfuromonas michiganensis sp. nov. Appl. Environ. Microbiol. 69, 2964-2974   DOI   ScienceOn
13 환경부. 2007. 2007년 지하수 수질측정망 운영결과
14 Muller, J.A., B.M. Rosner, G.V. Abendroth, G. Meshulam-Simon, P.L. McCarty, and A.M. Spormann. 2004. Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. Appl. Environ. Microbiol. 70, 4880-4888   DOI   ScienceOn
15 Sung, Y., K.E. Fletcher, K.M. Ritalahti, R.P. Apkarian, N. Ramos-Hernandez, R.A. Sanford, N.M. Mesbah, and F.E. Loffler. 2006. Geobacter lovleyi sp. nov. strain SZ, a novel metal-reducing and tetrachloroethene-dechlorinating bacterium. Appl. Environ. Microbiol. 72, 2775-2782   DOI   ScienceOn
16 Holliger, C., D. Hahn, H. Harmsen, W. Ludwig, W. Schumacher, B. Tindall, F. Vazquez, N. Weiss, and A.J.B. Zehnder. 1998. Dehalobacter restrictus gen. nov. and sp. nov., a strictly anaerobic bacterium that reductively dechlorinates tetra- and trichloroethene in an anaerobic respiration. Arch. Microbiol. 169, 313-321   DOI   ScienceOn
17 Kirk, J.L., L.A. Beaudette, M. Hart, P. Moutoglis, J.N. Klironomos, H. Lee, and J.T. Trevors. 2004. Methods of studying soil microbial diversity. J. Microbiol. Methods 58, 169-188   DOI   ScienceOn
18 Grostern, A. and E.A. Edwards. 2006. Growth of Dehalobacter and Dehalococcoides spp. during degradation of chlorinated ethanes. Appl. Environ. Microbiol. 72, 428-436   DOI   ScienceOn
19 Fantroussi, S., J. Mahillon, H. Naveau, and S.N. Agathos. 1997. Introduction of anaerobic dechlorinating bacteria into soil slurry microcosms and nested-PCR monitoring. Appl. Environ. Microbiol. 63, 806-811   PUBMED   ScienceOn
20 Middeldorp, P.J.M., M.L.G.C. Luijten, B.A.V.D. Pas, M.H.A.V. Eekert, S.W.M. Kengen, G. Schraa, and A.J.M. Stams. 1999. Anaerobic microbial reductive dehalogenation of chlorinated ethenes. Bioremed. J. 3, 151-169   DOI   ScienceOn
21 Gu, A.Z., B.P. Hedlund, J.T. Staley, S.E. Strand, and H.D. Stensel. 2004. Analysis and comparison of the microbial community structures of two enrichment cultures capable of reductively dechlorinating TCE and cis-DCE. Environ. Microbiol. 6, 45-54   DOI   ScienceOn
22 Lovley, D.R., D.E. Holmes, and K.P. Nevin. 2004. Dissimilatory Fe(III) and Mn(IV) reduction. Ad. Microb. Physiol. 49, 119-286   DOI   ScienceOn
23 Vogel, T.M., C.S. Criddle, and P.L. McCarty. 1987. Transformations of halogenated aliphatic compounds. Environ. Sci. Technol. 21, 722-736   DOI   PUBMED   ScienceOn
24 환경부. 2007. 2007 환경통계연감
25 Maymo-Gatell, X., Y.T. Chien, J.M. Gossett, and S.H. Zinder. 1997. Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. Science 276, 1568-1571   DOI   PUBMED   ScienceOn
26 Anderson, R.T., H.A. Vrionis, I. Ortiz-Bernad, C.T. Resch, P.E. Long, R. Dayvault, K. Karp, S. Marutzky, D.R. Metzler, A. Peacock, D.C. White, M. Lowe, and D.R. Lovley. 2003. Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer. Appl. Environ. Microbiol. 69, 5884-5891   DOI   ScienceOn
27 EPA. 2006. Toxicological profile for vinyl chloride
28 Drzyzga, O., J. Gerritse, J.A. Dijk, H. Elissen, and J.C. Gottschal. 2001. Coexistence of a sulphate-reducing Desulfovibrio species and the dehalorespiring Desulfitobacterium frappieri TCE1 in defined chemostat cultures grown with various combinations of sulphate and tetrachloroethene. Environ. Microbiol. 3, 92-99   DOI   ScienceOn
29 Holliger, C. 1995. The anaerobic microbiology and biotreatment of chlorinated ethenes. Curr. Opin. Biotechnol. 6, 347-351   DOI   ScienceOn
30 최정동, 배해득, 이동선, 안영호. 2006. 염화에텐으로 오염된 지하수의 혐기성 탈염소화와 미생물학적 군집의 특성. 한국폐기물학회 2006년도 정기총회 및 춘계학술연구발표회 논문집 Haz 05
31 Muyzer, G., E.C. de-Waal, and A.G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59, 695-700   PUBMED   ScienceOn
32 He, J., Y. Sung, R. Krajmalnik-Brown, K.M. Ritalahti, and F.E. Loffler. 2005. Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethenerespiring anaerobe. Environ. Microbiol. 7, 1442-1450   DOI   ScienceOn