• KSBB Journal
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• 제19권6호
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• pp.462-466
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• 2004

S. coelicolor A3(2)로부터 항산화 저분자 thiol분자인 MSH를 HPLC 및 monobromobimane 형광 검출 방법으로 분리${\cdot}$정제하여 그 존재를 확인하였다. 표준물질인 MSH-bimane과 동일하게 용출되는 MSH 분획을 확인하였으며 여러 thiol 분획 중 MSH 분획이 가장 많은 것으로 보아 MSH가 S. coelicolor의 주된 thiol 화합물로 판단되었다. MSH 생합성에 관여하는 효소 중 I-1-Pase의 유전자의 기능을 알아보기 위하여 이 유전자를 방선균에서 분리한 후 대장균에 클로닝하여 과도발현시켰다. 발현된 I-1-Pase를 Ni-NTA column을 사용하여 정제하였다. 정제된 I-1-Pase는 soluble protein으로 281개 아미노산으로 구성되어 있으며 분자량은 32 kDa이었다. 인간 및 대장균의 I-1-Pase와 각각 24와 $25\%$의 sequence homology를 보였으며, 기존의 I-1-Pase가 가지고 있는 공통의 I-1-Pase motif A와 motif B를 S. coelicolor A3(2)도 가지고 있는 것으로 확인되었다.

• BMB Reports
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• 제55권10호
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• pp.488-493
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• 2022

The specific pair of heat shock protein 70 (Hsp70) and Hsp40 constitutes an essential molecular chaperone system involved in numerous cellular processes, including the proper folding/refolding and transport of proteins. Hsp40 family members are characterized by the presence of a conserved J-domain (JD) that functions as a co-chaperone of Hsp70. Tumorous imaginal disc 1 (Tid1) is a tumor suppressor protein belonging to the DNAJA3 subfamily of Hsp40 and functions as a co-chaperone of the mitochondrial Hsp70, mortalin. In this work, we performed nuclear magnetic resonance spectroscopy to determine the solution structure of JD and its interaction with the glycine/phenylalanine-rich region (GF-motif) of human Tid1. Notably, Tid1-JD, whose conformation was consistent with that of the DNAJB1 JD, appeared to stably interact with its subsequent GF-motif region. Collectively with our sequence analysis, the present results demonstrate that the functional and regulatory mode of Tid1 resembles that of the DNAJB1 subfamily members rather than DNAJA1 or DNAJA2 subfamily proteins. Therefore, it is suggested that an allosteric interaction between mortalin and Tid1 is involved in the mitochondrial Hsp70/Hsp40 chaperone system.

• 대한가정학회지
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• 제44권1호
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• pp.101-113
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• 2006

The purpose of this study was to investigate and compare the characteristics of the motifs on cultural goods in In-Sa-Dong Street, Seoul, with those in Art Street, City Hall, Kwang-ju. For this study, 607 cultural goods were collected from the two cities: 265 in Seoul and 342 in Kwang-ju. Total cultural goods were classified by accessories, decoration pieces, stationery and ceramics and were studied by repetition patterns, motifs types, representative techniques, and representative types. The results were as follows. First, for repetition patterns of motifs, there were 219 simple repetition patterns in Seoul, and 289 in Kwang-ju, and 46 compound repetition patterns in Seoul, and 53 in Kwang-ju. The ratio of simple repetition pattern was higher than that of compound repetition pattern in both cities. Second, for motif types, floral, animal, letter, and graphic motifs were used far more frequently than any other motif types. Third, for representative techniques, embroidery was far more frequently used than any other representative techniques. Metallic crafts, chil-bo and paper techniques were also favored. Fourth, for representative types, realistic types were more common than abstract types.

• 복식문화연구
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• 제15권1호
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• pp.68-80
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• 2007

Surrealism's method of expression and inspiration has been a continuous influence on fashion. What is important is how to identify and research a source of inspiration, and how to use this inspiration to guide the designs that can be worn on a real human body. Therefore, the purpose of this study was to show how to develop ideas and designs from Surrealist objects and how to contribute to the development of designs to the diversity in visual and functional effects along with the changes in consumer's interest. Documentary studies about Surrealism and Surrealist-inflected fashion applying the works of Surrealist and fashion images as a tool of historical research were proceeded. And the ideas for theme, the motifs for print patterns, and the concept of designs were borrowed from Surrealist objects touching on the imagery between the real and reflected, optical and artificial, and the designs were developed with several different visual and functional effects changing color, pattern, size, and length with the function of zippers in each item. It also gives the person wearing it amusement because it includes the concept of play and D.I.Y. With effective planning, it is possible to attract consumer's interest in the market.

• Molecules and Cells
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• 제45권5호
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• pp.343-352
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• 2022

The advent of the assay for transposase-accessible chromatin using sequencing (ATAC-seq) has shown great potential as a leading method for analyzing the genome-wide profiling of chromatin accessibility. A comprehensive reference to the ATAC-seq dataset for disease progression is important for understanding the regulatory specificity caused by genetic or epigenetic changes. In this study, we present a genome-wide chromatin accessibility profile of 44 liver samples spanning the full histological spectrum of nonalcoholic fatty liver disease (NAFLD). We analyzed the ATAC-seq signal enrichment, fragment size distribution, and correlation coefficients according to the histological severity of NAFLD (healthy control vs steatosis vs fibrotic nonalcoholic steatohepatitis), demonstrating the high quality of the dataset. Consequently, 112,303 merged regions (genomic regions containing one or multiple overlapping peak regions) were identified. Additionally, we found differentially accessible regions (DARs) and performed transcription factor binding motif enrichment analysis and de novo motif analysis to determine new biomarker candidates. These data revealed the gene-regulatory interactions and noncoding factors that can affect NAFLD progression. In summary, our study provides a valuable resource for the human epigenome by applying an advanced approach to facilitate diagnosis and treatment by understanding the non-coding genome of NAFLD.

• Bulletin of the Korean Chemical Society
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• 제32권spc8호
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• pp.3009-3016
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• 2011

To investigate the possible isosteric replacement of imidazolidinone moiety in 4-phenyl-N-arylsulfonylimidazolidinone for broad and potent anticancer agents, a series of 4-phenyl-l(N)-arylsulfonylimidazolidinones 6a-k, imidazolidinethione analogs 7a-i, and imidazolidine oxime analogs 8a-c were prepared and evaluated for their in vitro anticancer activity against four human cancer cell lines (human lung A549, human colon COLO205, human leukemia K562, human ovary SK-OV-3). Among all the derivatives of N-arylsulfonylimidazolidinone 6a-k, compounds 6f and 6g showed the best inhibition comparable to doxorubicin against all cancer cell lines. Increasing the carbon chain on alkyl moieties of carbamates as shown in 6c-g did not alter the activity. The imidazolidinethione analogs 7a-i and imidazolidin-2-one oxime derivatives 8a-c did not possess any good activity. Therefore, imidazolidinone moiety is the best pharmacophore among the 4-phenyl-Narylsulfonylimidazolidinone derivatives.

• 한국생활과학회지
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• 제8권3호
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• pp.565-577
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• 1999

In this world of globalism, achieving the winning position in the competitive international market is essential in the area of fashion industry. For this reason, we should accomplish this task by adapting our own traditional beauty to the modern fashion design through the modernization of the Korean traditional style. Therefore, this study attempted to analyze the fashion design works of the active Korean fashion designer, Sul Yoon-Hyung, focusing on the subject how she incorporated the modern fashion design with the traditional motives. In order to study this research subject, both quantitative and qualitative research methods were used to collect and analyse the textile motives used in the designer's works found from the major fashion magazines, Bazaar, Vogue, Marie Claire, ELLE, WWD, and Fashion Today during the last seven years(1993-1999). The collected research data, 68 works, was counted and analyzed in terms of the categories of Korean traditional motives they were adapted from, the application techniques, and the aesthetic characteristics. As a result of the study, it was found that Sul Yoon-Hyung has adapted different kinds of Korean traditional motives including motives borrowed from the nature such as flowers and animals, geometrical forms, and Chinese letters with different specific symbolic meanings for each motif and many different techniques were utilized to apply these motives. It was apparent to note that the Korean traditional motives were successfully incorporated into her fashion design works expressing the beauty of oriental grace, naturalism, sophistication, and above all, the modern sense.

• 한국정보과학회 언어공학연구회:학술대회논문집(한글 및 한국어 정보처리)
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• 한국정보과학회언어공학연구회 1989년도 한글날기념 학술대회 발표논문집
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• pp.183-190
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• 1989

본 논문에서는 한글 X 윈도우 시스템의 개발 과정을 소개하고 이용 바탕으로 한 GUI의 한글화 방안을 제시한다. X 윈도우 시스템은 이식성이 높고 다양한 사용자 접속을 제공한다. 특히 소스가 거의 공개되어 있어 UNIX 워크스테이션의 표준 윈도우 시스템으로서의 위치를 굳혀가고 있다. 한글 X는 여기에 한글의 입출력 기능을 추가한 것이다. 현재 UNIX 와 X 윈도우를 기반으로 한 그래픽 사용자 접속 (GUI)을 표준화하려는 움직임이 세계적으로 높아가고 있다. OSF와 UI의 두 단체에서 각각 Motif와 OpenLook을 표준 GUI로 내놓고 있는데 이 두 GUI는 모두 나름대로의 지지 기반을 가지고 사용될 것으로 보인다. 때문에 한글 GUI의 개발은 시급하고 중요한 문제라고 할 수 있다. 한글 X의 개발은 X 윈도우 환경에서 한글을 사용할 수 있게 되었다는 것뿐만 아니라 GUI 한글화의 기반을 마련했다는 점에서 그 의의를 갖는다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.195-195
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• 2004

To discover germ cell-specific transcripts, we prepared a cDNA library from adult testes of 35-day old mice and subtracted it with mRNA from the testes of juvenile mice. Real-time RT-PCR analysis indicated that 42 cDNA clones in the subtracted library were expressed more intensely in the adult testes than in the juvenile testes. One clone identified by subtraction is expressed preferentially in the late spermatid and is located on chromosome 17E3 in mouse and 2p22 in human. (omitted)

• Molecules and Cells
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• 제40권12호
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• pp.954-965
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• 2017

Mammalian genomes are well established, and highly conserved regions within odorant receptors that are unique from other G-protein coupled receptors have been identified. Numerous functional studies have focused on specific conserved amino acids motifs; however, not all conserved motifs have been sufficiently characterized. Here, we identified a highly conserved 18 amino acid sequence motif within transmembrane domain seven (CAS-TM7) which was identified by aligning odorant receptor sequences. Next, we investigated the expression pattern and distribution of this conserved amino acid motif among a broad range of odorant receptors. To examine the localization of odorant receptor proteins, we used a sequence-specific peptide antibody against CAS-TM7 which is specific to odorant receptors across species. The specificity of this peptide antibody in recognizing odorant receptors has been confirmed in a heterologous in vitro system and a rat-based in vivo system. The CAS-TM7 odorant receptors localized with distinct patterns at each region of the olfactory epithelium; septum, endoturbinate and ectoturbinate. To our great interests, we found that the CAS-TM7 odorant receptors are primarily localized to the dorsal region of the olfactory bulb, coinciding with olfactory epithelium-based patterns. Also, these odorant receptors were ectopically expressed in the various non-olfactory tissues in an evolutionary constrained manner between human and rats. This study has characterized the expression patterns of odorant receptors containing particular amino acid motif in transmembrane domain 7, and which led to an intriguing possibility that the conserved motif of odorant receptors can play critical roles in other physiological functions as well as olfaction.