• 제목/요약/키워드: histological section

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Falciform ligament(간겸상인대)에서 관찰되는 프리모 조직의 조직학적 특성 연구 (Histological study of the primo vascular system on the falciform ligament)

  • 연선희;권오상;이새봄;조성진;최광호;이상훈;최선미;류연희
    • 한국한의학연구원논문집
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    • 제18권2호
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    • pp.131-137
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    • 2012
  • Objectives : Primo vascular system is known to new circulatory system and suggested as a anatomical structure of meridian system. Primo-vessels are present throughout the whole body. The purpose of this study is to identify primo tissues taken from falciform ligament and to compare with organ surface primo tissue, blood vessel and lymph vessel. Methods : Male Sprague-Dawley rats (8weeks old, 250~320g) used for this study. The medial line of the abdomen was dissected and searched for primo tissues in falciform ligament and on the internal organs using stereomicroscope. We performed serial cross section and histological investigations. The tissues were stained with hematoxylin-eosin and Masson's trichrome. Results : 1. The primo tissues attached on the falciform ligament had white color and length of 5~8mm. 2. We could observe primo tissues are classified with ligament tissues. 3. Histologically, primo tissue on falciform ligament and organ surface primo tissue could be considered same tissue. Conclusions : In this study, we observed primo tissue discovered on the falciform ligament. And we also histologically compared such discovered primo tissue with organ surface primo tissue. Consequently, we could consider that two tissues have histological similarity and possibility of connection in one network system.

The influence of leukocyte-platelet-rich plasma on accelerated orthodontic tooth movement in rabbits

  • Nakornnoi, Theerasak;Leethanakul, Chidchanok;Samruajbenjakun, Bancha
    • 대한치과교정학회지
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    • 제49권6호
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    • pp.372-380
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    • 2019
  • Objective: To determine the effects of a local injection of leukocyte-platelet-rich plasma (L-PRP) on orthodontic tooth movement in rabbits. Methods: Twenty-three male New Zealand white rabbits were included in a split-mouth design. Tooth movement with a 100-g nickel-titanium closed-coil spring was performed on the maxillary first premolars. L-PRP was injected submucosally at the buccal and lingual areas of the first premolar in one random side of the maxilla and the other side served as the control and received normal saline. The amount of tooth movement was assessed on three-dimensional digital models on days 0, 3, 7, 14, 21, and 28. Histological findings and osteoclast numbers were examined on day 0 as the baseline and on days 7, 14, and 28. Results: The L-PRP group showed significantly greater cumulative tooth movement at all observed periods. However, a significantly higher rate of tooth movement was observed only on days 0-7 and 7-14. The osteoclast numbers were significantly increased in the L-PRP group on days 7 and 14. Conclusions: Local injection of L-PRP resulted in a transient increase in the rate of tooth movement and higher osteoclast numbers.

현사시나무 3배체의 핵 DNA 함량 및 조직학적 특성 (The Nuclear DNA Content and Histological Characteristics of Triploid Poplars Grown In Vitro)

  • 배은경;이효신;이재순;최영임;박소영
    • 한국산림과학회지
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    • 제102권2호
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    • pp.198-203
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    • 2013
  • 바이오매스 생산과 분자육종 연구를 위한 소재로 육성된 3배체 현사시나무(Populus alba ${\times}$ P. glandulosa $F_1$)의 핵 DNA 함량과 조직학적 특성을 조사하였다. 유세포 분석 결과 3계통의 3배체 현사시나무(Line-1, Line-17, Line-18)의 핵 DNA 함량이 2배체 현사시나무에 비하여 1.6배 이상 큰 것으로 나타났다. 조직학적 분석 결과 3배체 현사시나무는 계통별로 조금 다른 차이를 나타내었지만 'ine-18'은 줄기 단면적이 2배체 현사시나무에 비해 1.6배 이상 넓고, 수와 피층/사부의 면적이 2.0배와 1.6배 이상 각각 증가한 것으로 나타났다. 또한 'ine-18'은 잎의 공변세포의 길이와 면적이 2배체에 비하여 1.2배 이상 증가한 것으로 나타났다. 본 연구결과는 3배체 포플러의 계통 육성을 통한 바이오매스 생산 및 산업적 이용 가능성을 탐색하기 위한 기초자료로 이용될 것으로 기대된다.

Overview of Benign and Malignant Prostatic Disease in Pakistani Patients: A Clinical and Histopathological Perspective

  • Arshad, Huma;Ahmad, Zubair
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권5호
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    • pp.3005-3010
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    • 2013
  • Background: To present the overall clinical and histological perspective of benign and malignant prostatic disease as seen in our practice in the Section of Histopathology, Department of Pathology and Microbiology, Aga Khan University Hospital, Karachi, Pakistan. Materials and Methods: All consecutive prostate specimens (transurethral resection or TUR, enucleation, needle biopsies) received between July 1, 2012 and December 31, 2012 were included in the study. Results: Of the total of 785 cases, 621 (79.1%) were TUR specimens, 80 (10.2%) enucleation specimens, and 84 (10.7%) needle biopsies. Some 595 (75.8%) were benign, while 190 (24.2%) were malignant. Mean weight of BPH specimens was 19 grams and 43 grams for TUR and enucleation specimens respectively. Almost 67% of adenocarcinomas were detected on TUR or enucleation specimens. Of the above cases, 41.7% were clinically benign while 58.3% were clinically malignant. The average volume of carcinoma in all cases ranged between 60 to 65%. The average number of cores involved in needle biopsies was 5. In general, higher Gleason scores were seen in TUR/enucleation specimens than in needle biopsies. Overall, in all types of specimens, commonest Gleason score was 7, seen in 74 (38.9%) cases, followed by Gleason score 9 seen in 47 (24.7%) cases. Out of the 63 needle biopsies with carcinoma, radical prostatectomy was performed in 16 cases (25.4%). Conclusions: Benign prostatic hyperplasia (BPH) is extremely common and constitutes the bulk of prostate specimens. TMajority of prostatic carcinomas are still diagnosed on TUR or enucleation specimens. These included both clinically benign and clinically malignant cases. The volume of carcinoma in these specimens was quite high indicating extensive disease. Gleason scores were also generally high compared with scores from needle biopsies. Commonest Gleason score in all type of specimens was 7. Pathologic staging was possible in very few cases since radical prostatectomies are rarely performed.

Germ Cell Apoptosis in the Testis of Transgenic Pigs

  • Chung, Hak-Jae;Kim, Bong-Ki;Ko, Yeoung-Gyu;Woo, Jei-Hyun;Kim, Jeom-Soon;Jung, Jin-Kwan;Chang, Won-Kyong
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2004년도 춘계학술발표대회
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    • pp.233-233
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    • 2004
  • PURPOSE: Gene expression and apoptosis in testicular germ cells has been demonstrated in many transgenic animals. However, little is known about the transgenic pig and rates of apoptosis during spermatogenesis. METHODS : Morphological and biochemical features of apoptosis reported in other species were used to confirm that the TdT-mediated dUTP Nick end labeling (TUNEL) assay is an acceptable mothos for idendtification and quantification of apoptotic transgenic germ cells in histological tissue section from transgenic pig testis. (omitted)

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회양목의 생약학적 연구 (Pharmacognostical Studies on Bux Wood)

  • 이선주;김정희
    • 생약학회지
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    • 제2권1호
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    • pp.15-18
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    • 1971
  • The histological structures of Buxus koreana were studied by cross section and compared with those of Buxus koreana var. elongata, B. koreana var. insularis, B. microphylla subsp. sinica (Taiwan) and B. liukiuensis(Okinawa). Many different points showed that B. koreana var. insularis is similar to B. microphylla subsp. sinica while B. koreana to B. liukiuensis. The extracts of Buxus koreana by three solvents are as follows: ether ext. 3.34%, dilute alcohol ext. 16.98%, water ext. 11.67%. The total alkaloid of Buxus koreana is approximately 0.01%. The general pharmacological effects of dilute alcohol ext. of Buxus koreana were studied and the following conclusions were obtained. Contraction of excised uterus of rabbit was augmented by treatment of dilute alcohol ext. Motility of excised intestine of rabbit was stopped for a moment and stimulated at once by treatment of dilute alcohol ext.

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A Forgotten Entity following Breast Implant Contracture: Does Baker Need a Change?

  • Pagani, Andrea;Aitzetmuller, Matthias M.;Larcher, Lorenz
    • Archives of Plastic Surgery
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    • 제49권3호
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    • pp.360-364
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    • 2022
  • Although capsular contracture represents one of the most important complications after breast augmentation, local inflammation and fibrosis can lead, to capsular calcification, an often-forgotten radiological sign of capsular contracture. In this article, the authors present a clinical case of breast implant calcification in an 81-year-old patient. Although this complication has been rarely described, the literature was reviewed to clarify the role of the local microenvironment in capsular contracture and calcification. At present, capsular contracture patients are classified using the conventional Baker score and the histological Wilflingseder classification. As it was not possible to consider capsular calcification when classifying our patient using the traditional scores, the authors propose an updated version of the current scale.

The Effect of Silk Membrane Plus 3% 4-hexylresorcinol on Guided Bone Regeneration in a Rabbit Calvarial Defect Model

  • Seok, Hyun;Lee, Sang-Woon;Kim, Seong-Gon;Seo, Dong-Hyun;Kim, Han Sung;Kweon, Hae Yong;Jo, You-Young;Kang, Tae Yeon;Lee, Myung-Jin;Chae, Weon-Sik
    • International Journal of Industrial Entomology and Biomaterials
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    • 제27권1호
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    • pp.209-217
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    • 2013
  • The objective of this study was to evaluate the bone regeneration capability of silk membrane plus 3% 4-hexylresorcinol (3% 4-HR plus SM) in a rabbit calvarial defect model. Twenty New Zealand white rabbits were used in this study. Bilateral round shaped defects were created in the parietal bone (diameter: 8.0 mm). And the defects were covered with (1) 3% 4-HR plus SM, (2) collagen membrane (CM), (3) no graft material. After surgery, the animals were sacrificed at 4 weeks and 8 weeks. Bone regeneration was analyzed in each section by micro-computerized tomography (${\mu}$-CT). And Hematoxylin and eosin stains were used for histological analysis. As measured by ${\mu}$-CT analysis 4 weeks after surgery, the average of new bone formation in animals treated with 3% 4-HR plus SM was greater than that of animals treated with CM. and the difference was statistically significant. And well organized lamella bones were observed in the histological view of the 3% 4-HR plus SM group. Therefore, more bone regeneration was seen in animals treated with 3% 4-HR plus SM than in those treated with CM or uncovered control.

해부병리조직에 대한 칼라 영상분석 (Color Image Analysis of Histological tissue Sections)

  • 최흥국
    • 한국정보처리학회논문지
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    • 제6권1호
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    • pp.253-260
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    • 1999
  • 본 논문에서는 조합된 텍스쳐와 칼라 정보로부터 다변수의 선형 구별 알고리즘을 사용하여 영상분할에 대한 새로운 방법론의 개발을 제시한다. 그 칼라 텍스쳐는 칼라 영상의 공간과 색깔의 밴드로부터 한 화소가 갖는 3X3의 마스크에서 Haralick 과 Pressman의 텍스쳐 특성들을 계산했다. 모두 9X28개의 텍스쳐 특성들 중에서 학습을 기반으로 크게 식별자(classifier)에 영향을 주는 특성들을 도출하였으며 결과적으로 뽑혀진 10개의 특성이 한 영상을 4부분으로 분할하는데 사용되어졌다. 이 방법론의 결과로 얻어진 영상은 고전적인 칼라와 텍스쳐 분할 방법론의 상자식별자(Box Classifier)와 Maximum Likelihood 식별기들과 비교했다. 이것은 Fastred-Lightgreen으로 염색된 전립선암이 조직에서 얻은 영상을 통해 비교를 했을 경우에 잘 나타난다. 학습 데이터를 통해 나타난 이 새로운 방법론은 97.5%의 정확한 식별성으로 또한 검증된 최상의 방법론중의 하나이다. 이 결과들이 더 많은 영상에 사용된다면, 이 방법론은 칼라와 텍스쳐가 분할에 관련됨으로써 보다 정확한 영상을 분할하기 위한 효율적인 도구가 될 것이다.

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Progesterone의 이화(異化)효소, 20α-hydroxysteroid dehydrogenase의 활성 및 활용에 관한 연구 II. 비장내(脾臟內) macrophage의 활성 및 난소에서의 조직학적 변화 (Activity and application of 20α-hydroxysteroid dehydrogenase in rat II. Changes in activities of the splenic macrophages and ovarian histological findings)

  • 강정부;곽수동
    • 대한수의학회지
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    • 제34권4호
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    • pp.873-880
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    • 1994
  • Progesterone catabolizing enzyme, the enzyme $20{\alpha}$-hydroxysteroid deltydrgenase($20{\alpha}$-HSD) is pivotal in the regulation of ovarian function in rodents, which catabolizes progesterone into biologically inactive $20{\alpha}$ hydroxypregn-4-en-3-one($20{\alpha}$-OHP). In this study was carried out the influence of $20{\alpha}$-HSD activity to ovarian function and regulation such as ovulation, formation of corpus luteum, maintenance of estrous cycles and pregnancy, we investigated changes in activities of the splenic macrophages and ovarian histological findings in rat. During the estrous cycles, the ratious of phagocytotic macrophage in splenic adherent cells were highest on the proestrous, but they were lowest on metestrous. During the pregnancy, the ratious of phagocytotic macrophages in splenic adhrent cells were lowest by 1 day and then significantly increased toward the pregnancy, which were highest pregnancy on day 12. On histological findings in rat ovary, the mean number of growing, antral and mature follicles were 15.9(72.9%), 3.4(15.9%), and 2.4(11.2%), respectively. Growing follicles reached to lowest number at diestrus and mature follicle reached to lowest number at metestrus. The numbers of corpus luteums per tissue section of the ovary were 14.4 and the number of normal and atretic follicles were 11.0(76.4%) and 3.4(23.6%), respectively. The number of corpus luteum with vacolated cell were 1.7(11.8%). In this study suggests splenic macrophages are as a source of the substance which maintainer progesterone secretion from luteal cells, and are recognized pass the vessels and reside in the fresh corpora lutea soon after ovulation via inflammatory reactions, and these macrophages are felt to have a stimulatory effect on the formation of cropora lutea. In view of the results include previous report, ovarian $20{\alpha}$-hydroxysteroid dehydrogenase enzyme and splenic macrophages consider play central role in the control and maintenance of estrous cycles and pregnancy, and also applicable to both clinical and research in a wide variety such as control of reproductive system.

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