Germ Cell Apoptosis in the Testis of Transgenic Pigs

  • Chung, Hak-Jae (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstration) ;
  • Kim, Bong-Ki (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
  • Ko, Yeoung-Gyu (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
  • Woo, Jei-Hyun (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
  • Kim, Jeom-Soon (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
  • Jung, Jin-Kwan (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstratio) ;
  • Chang, Won-Kyong (Animal Biotechnology Division, National Livestock Research Institute, Rural Development Adminstration)
  • Published : 2004.06.01

Abstract

PURPOSE: Gene expression and apoptosis in testicular germ cells has been demonstrated in many transgenic animals. However, little is known about the transgenic pig and rates of apoptosis during spermatogenesis. METHODS : Morphological and biochemical features of apoptosis reported in other species were used to confirm that the TdT-mediated dUTP Nick end labeling (TUNEL) assay is an acceptable mothos for idendtification and quantification of apoptotic transgenic germ cells in histological tissue section from transgenic pig testis. (omitted)

Keywords