한국산림과학회지 (Journal of Korean Society of Forest Science)

  • 제102권2호
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  • Pages.198-203
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  • 2013
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  • 2586-6613(pISSN)
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  • 2586-6621(eISSN)
한국산림과학회 (Korean Society of Forest Science)
권호 표지

현사시나무 3배체의 핵 DNA 함량 및 조직학적 특성

The Nuclear DNA Content and Histological Characteristics of Triploid Poplars Grown In Vitro

  • 배은경 (국립산림과학원 산림생명공학과) ;
  • 이효신 (국립산림과학원 산림생명공학과) ;
  • 이재순 (국립산림과학원 산림생명공학과) ;
  • 최영임 (국립산림과학원 산림생명공학과) ;
  • 박소영 (충북대학교 원예과학과)
  • Bae, Eun-Kyung (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Lee, Hyoshin (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Lee, Jae-Soon (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Choi, Young-Im (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Park, So-Young (Department of Horticultural Science, Chungbuk National University)
  • 발행 : 2013.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

바이오매스 생산과 분자육종 연구를 위한 소재로 육성된 3배체 현사시나무(Populus alba ${\times}$ P. glandulosa $F_1$)의 핵 DNA 함량과 조직학적 특성을 조사하였다. 유세포 분석 결과 3계통의 3배체 현사시나무(Line-1, Line-17, Line-18)의 핵 DNA 함량이 2배체 현사시나무에 비하여 1.6배 이상 큰 것으로 나타났다. 조직학적 분석 결과 3배체 현사시나무는 계통별로 조금 다른 차이를 나타내었지만 'ine-18'은 줄기 단면적이 2배체 현사시나무에 비해 1.6배 이상 넓고, 수와 피층/사부의 면적이 2.0배와 1.6배 이상 각각 증가한 것으로 나타났다. 또한 'ine-18'은 잎의 공변세포의 길이와 면적이 2배체에 비하여 1.2배 이상 증가한 것으로 나타났다. 본 연구결과는 3배체 포플러의 계통 육성을 통한 바이오매스 생산 및 산업적 이용 가능성을 탐색하기 위한 기초자료로 이용될 것으로 기대된다.

Herein we analyzed the nuclear DNA content and the histological characteristics of the triploid of the 'Hyunsasi' (Populus alba ${\times}$ P. glandulosa $F_1$) which were developed for biomass production and molecular breeding research. The flow cytometric analysis showed that the nuclear DNA content of the 3 triploids were 1.6 times greater than those of the diploid. In terms of histological characteristics, the cross-section area of the stem of 'Line-18' was 1.6 times larger than that of the diploid. The area of pith, and cortex and phloem of the stem of 'Line-18' was also 1.6 and 2.0 times larger than that of the diploid, respectively. Moreover, the length and area of guard cell of 'Line-18' was 1.2 times larger than that of the diploid. These results helps to understand the cytological characteristics of the triploid poplar clones, and further investigations in the growth rate and wood properties of the triploids growing in the field will determine whether the triploid poplars are good candidates for molecular breeding programs and for the improvement of industrial biomass productivity.

키워드

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