• 제목/요약/키워드: high-yield strains

검색결과 158건 처리시간 0.031초

생육온도에 따른 느타리버섯 자실체의 형태 및 재배적 특성변화 (The effect of temperature on morphological of fruiting body and cultivated characteristics of oyster mushroom)

  • 전창성;윤형식;장갑열;공원식;이강효;이찬중
    • 한국버섯학회지
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    • 제9권4호
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    • pp.155-160
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    • 2011
  • 본 연구는 느타리버섯 자실체가 생육온도에 따른 수량성과 자실체의 형태적 특성을 확인하기 위하여 느타리버섯 품종 중에서 저온, 중온, 고온성 품종을 구분하여 12개 품종에 대하여 실험을 실시하였다. 수량성에 있어서는 품종의 온도 반응적 특성에 맞는 온도에서 수량성이 가장 높게 나타났다. 형태적 특성에 있어서는 갓크기는 온도처리에 따른 일정한 경향을 보이지 않았으며, 갓두께에 있어서는 저온 처리인 $10^{\circ}C$에서 품종에 관계없이 가장 두껍게 나타났다. 대길이는 삼복 외 4품종은 온도가 증가하면서 같이 증가하였으며, 그 외의 품종은 품종에 따라 약간의 차이를 보였다. 대굵기에 있어서는 대체적으로 온도 증가에 따라 가늘어지는 경향이나 품종간 약간의 차이가 나타났다. 버섯수확소요일수에서는 $10^{\circ}C$처리에서 가장 기간이 길었으며, 품종별 가장 짧은 소요일수를 보인 것은 품종의 온도 특성에 따라 다르게 나타났다. 품종에 따라서 온도에 반응하여 기형적 형태를 보이는 경우가 있었는데 이런 특이적 현상은 수한1호의 경우 저온 처리인 $10^{\circ}C$에서 버섯이 발이후 어린 버섯 갓 표면에 백색 인피가 형성하며 성장 후에는 갓의 일부분에만 발생하거나 없어지는 현상이 있었으며, 춘추2호에서는 고온인 $20^{\circ}C$ 이상에서는 대가 뒤틀리며, 대기 휘어지는 특이적 현상이 발생하였다.

Isolation and Characterization of Monokaryotic Strains of Lentinula edodes Showing Higher Fruiting Rate and Better Fruiting Body Production

  • Ha, Byeong-Suk;Kim, Sinil;Ro, Hyeon-Su
    • Mycobiology
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    • 제43권1호
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    • pp.24-30
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    • 2015
  • The effects of monokaryotic strains on fruiting body formation of Lentinula edodes were examined through mating and cultivation of the mated dikaryotic mycelia in sawdust medium. To accomplish this, monokaryotic strains of L. edodes were isolated from basidiospores of the commercial dikaryotic strains, Chamaram (Cham) and Sanjo701 (SJ701). A total of 703 matings (538 self-matings and 165 outcrosses) were performed, which generated 133 self-mates and 84 outcross mates. The mating rate was 25% and 50% for self-mating and outcross, respectively. The bipolarity of the outcross indicated the multi-allelic nature of the mating type genes. The mating was only dependent on the A mating type locus, while the B locus showed no effect, implying that the B locus is multi-allelic. Next, 145 selected dikaryotic mates were cultivated in sawdust medium. The self-mated dikaryotic progenies showed 51.3% and 69.5% fruiting rates for Cham and SJ701, respectively, while the fruiting rate of the outcross mates was 63.2%. The dikaryotic mates generated by mating with one of the monokaryotic strains, including A20, B2, E1, and E3, showed good fruiting performance and tended to yield high fruiting body production, while many of the monokaryotic strains failed to form fruiting bodies. Overall, these findings suggest that certain monokaryotic strains have traits enabling better mating and fruiting.

돼지의 웅돈계열에 따른 도체형질 및 육질 변화에 관한 연구 (Breed Effects of Terminal Sires on Carcass Traits and Meat Quality in Commercial Pig Industry)

  • 선두원;서현우;김병우;양한술;주선태;이정규
    • 한국축산식품학회지
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    • 제33권1호
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    • pp.125-132
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    • 2013
  • 본 연구는 돼지의 웅돈 계열에 따른 효과를 알아보기 위하여 425두에 대하여 도체특성, 부분육 생산량 및 육질특성을 조사하였다. 웅돈계열은 수입국과 품종에 따라 5계열(D1, D2, B1, B2, BD)로 나누었다. 결과를 살펴보면, D1계열과 D2계열에서 높은 1+등급 출현율을 보였고, 육량등급에서는 B1, B2, D1계열에서 높은 A등급 출현율을 보였다. 부분육 생산량에서 D1계열이 목심, 갈비, 앞다리, 등심, 삼겹살, 안심에서 많은 부분육 생산량을 보였다. 일반성분에서 수분함량은 B1계열에서 가장 높은 수분함량을 보였으며, D1계열에서 유의적으로 높은 단백질함량과 지방함량은 보였다(p<0.05). 전단가는 B1계열에서 유의적으로 낮은 값을 보였다(p<0.05). 육색은 D1, D2, BD계열에서 유의적으로 높은 명도 값을 보였고, B2계열에서 유의적으로 낮은 값을 보였으며, D1, D2, BD계열에서 유의적으로 높은 황색도 값을 보였다(p<0.05). 관능평가는 D1과 D2계열에서 유의적으로 낮은 육즙 삼출을 보였고, 마블링과 종합적인 기호도에서 유의적으로 높은 값을 보였다(p<0.05). 따라서 본 연구의 결과를 종합해 볼 때 웅돈의 선택에 있어서 1+등급 출현율이 높고, 삼겹살 중량이 많고, 명도 값이 높으며, 관능평가에서 좋은 결과를 보여준 D1과 D2계열을 이용하는 것이 생산자 및 농가 소득에 도움이 될 것으로 사료된다.

Substrate-Dependent Auxin Production by Rhizobium phaseoli Improves the Growth and Yield of Vigna radiata L. Under Salt Stress Conditions

  • Zahir, Z.A.;Shah, M. Kashif;Naveed, M.;Akhter, M. Javed
    • Journal of Microbiology and Biotechnology
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    • 제20권9호
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    • pp.1288-1294
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    • 2010
  • Rhizobium phaseoli strains were isolated from the mung bean (Vigna radiata L.) nodules, and the most salt tolerant and high auxin producing rhizobial isolate N20 was evaluated in the presence and absence of L-tryptophan (L-TRP) for improving the growth and yield of mung bean under saline conditions in a pot experiment. Mung bean seeds were inoculated with peat-based inoculum and NP fertilizers were applied at 30-60 kg/ha, respectively. Results revealed that imposition of salinity reduced the growth and yield of mung bean. On the contrary, the separate application of L-TRP and Rhizobium appeared to mitigate the adverse effects of salt stress. However, their combined application produced more pronounced effects and increased the plant height (28.2%), number of nodules per plant (71.4%), plant biomass (61.2%), grain yield (65.3%), and grain nitrogen concentration (22.4%) compared with untreated control. The growth promotion effect might be due to higher auxin production in the rhizosphere and improved mineral uptake that reduced the adverse effects of salinity. The results imply that supplementing Rhizobium inoculation with L-TRP could be a useful approach for improving the growth and yield of mung bean under salt stress conditions.

Isolation, Root Colonization and Evaluation of Some Plant Growth-promoting Rhizobacteria in Paddy Rice

  • Kang, Ui-Gum;Park, Hyang-Mi;Ko, Jee-Yeon;Lee, Jae-Saeng;Jeon, Weon-Tai;Park, Chang-Young;Park, Ki-Do;Chebotar, Vladimir K.
    • 한국토양비료학회지
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    • 제50권3호
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    • pp.135-149
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    • 2017
  • In order to obtain promising rice growth-promoting microbial strains that can be used as substitutes for chemical fertilizers, 172 bacterial strains were isolated from rice roots grown in Korean and Russian soils. Out of them, the strains KR076, KR083, KR181 and RRj228 showed plant growth-promoting activities on maize seedlings. Bacillus megaterium KR076 and Bacillus sp. KR083 showed both nitrogen-fixing and plant growth-promoting activities, while Rhizobium sp. KR181 and Pseudomonas sp. RRj228 appeared to support only plant growth-promotion, but not $N_2$ fixation. Especially, RRj228 showed high growth promoting activity at low concentrations. Inoculation studies with KR083 and RRj228 revealed a high affinity to the Japonica rice variety such as Junambyeo than the Korean Tongil type variety such as Arumbyeo. Both KR083 and RRj228 strains showed rhizoplane and/or endophytic colonization in Japonica and Tongil types rice when soaked with the bacterial suspension of $1.1{\times}10^5cfu\;ml^{-1}$ for six and twelve hours. However, the total bacterial cell numbers were higher in the roots of Japonica variety than in the Tongil type. In inoculation trials with Daesanbyeo rice variety, the seedlings inoculated with KR181 and RRj228 at the rate of $2.0{\times}10^6cfu\;ml^{-1}$ showed yield increment of 35% and 33% (p < 0.01), respectively, so that they contributed to the replacement of chemical fertilizer at half doses of N, $P_2O_5$, and $K_2O$ in pots. In Junambyeo rice seedlings, the strain RRj228, when inoculated with a cell suspension of $1.8{\times}10^6cfu\;ml^{-1}$, promoted 3.4% higher yield at 70% dose than at a full dose level of N $110kg\;ha^{-1}$ in field. These results suggest that the rhizobacteria KR181 and RRj228 are prospective strains for enhancing rice performance.

Effects of Glycerol and Shikimic Acid on Rapamycin Production in Streptomyces rapamycinicus

  • La, Huyen Thi Huong;Nguyen, Thao Kim Nu;Dinh, Hang Thuy;Nguyen, Quyen Minh Huynh;Nguyen, Minh Hong
    • 한국미생물·생명공학회지
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    • 제48권3호
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    • pp.296-302
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    • 2020
  • Rapamycin, derived from Streptomyces rapamycinicus, is an important bioactive compound having a therapeutic value in managing Parkinson's disease, rheumatoid arthritis, cancer, and AIDS. Because of its pharmaceutical activity, studies over the past decade have focused on the biosynthesis of rapamycin to enhance its yield. In this study, the effect of rapG on rapamycin production was investigated. The rapG expression vector was constructed by utilizing the integration vector pSET152 under the control of the erythromycin resistance gene (ermE), a strong constitutive promoter. The rapamycin yield of wild type (WT) and WT/rapG overexpression mutant strains, under fermentation conditions, was analyzed by high-performance liquid chromatography (HPLC). Our results revealed that overexpression of rapG increased rapamycin production by approximately 4.9-fold (211.4 mg/l) in MD1 containing 15 g/l of glycerol, compared to that of the WT strain. It was also found that Illicium verum powder (10 g/l), containing shikimic acid, enhanced rapamycin production in both WT and WT/rapG strains. Moreover, the amount of rapamycin produced by the WT/rapG strain was statistically higher than that produced by the WT strain. In conclusion, the addition 15 g/l glycerol and 15 g/l I. verum powder produced the optimal conditions for rapamycin production by WT and WT/rapG strains.

SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus

  • Oh, Youn-Lee;Choi, In-Geol;Jang, Kab-Yeul;Kim, Min-Seek;Oh, Min ji;Im, Ji-Hoon
    • Mycobiology
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    • 제49권6호
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    • pp.589-598
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    • 2021
  • White strains of Hypsizygus marmoreus are more difficult to cultivate than are brown strains; therefore, new white strain breeding strategies are required. Accordingly, we constructed the genetic map of H. marmoreus with 1996 SNP markers on 11 linkage groups (LGs) spanning 1380.49 cM. Prior to analysis, 82 backcrossed strains (HM8 lines) were generated by mating between KMCC03106-31 and the progenies of the F1 hybrid (Hami-18 × KMCC03106-93). Using HM8, the first 23 quantitative trait loci (QTLs) of yield-related traits were detected with high limit of detection (LOD) scores (1.98-9.86). The length, thickness, and hardness of the stipe were colocated on LG 1. Especially, length of stipe and thickness of stipe were highly correlated given that the correlation coefficients were negative (-0.39, p value ≤ .01). And a typical biomodal distribution was observed for lightness of the pileus and the lightness of the pileus trait belonged to the LG 8, as did traits of earliness and mycelial growth in potato dextrose agar (PDA) medium. Therefore, results for color traits can be suggested that color is controlled by a multi-gene of one locus. The yield trait was highly negatively correlated with the traits for thickness of the stipe (-0.45, p value ≤ .01). Based on additive effects, the white strain was confirmed as recessive; however, traits of mycelial growth, lightness, and quality were inherited by backcrossed HM8 lines. This new genetic map, finely mapped QTLs, and the strong selection markers could be used in molecular breeding of H. marmoreus.

구강에서 분리된 세균에 대한 오미자(Schizandra chinensis Baill) 추출물의 영향 (Effect of the Extract of Schizandra chinensis Baill on Bacteria Isolated from Oral Cavity)

  • 정현자;이영애;지원대
    • 치위생과학회지
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    • 제2권2호
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    • pp.85-88
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    • 2002
  • 구강에서 분리한 세균에 대한 오미자 추출물의 증식저해를 조사하였다. 물을 추출용매로 사용할 때의 추출수율은 53.8%로 메탄올보다 높았고, 추출온도가 높은 것이 보다 유리하였다. 구강에서 분리된 세균의 생육에 대한 오미자 물추출물의 영향을 조사한 결과, 오미자 물추출물은 강한 항균력을 보였다. 구강에서 분리된 세균에 대한 오미자 물추출물의 최소저해 농도는 ${125{\sim}1000{\mu}g/ml}$의 범위인 것으로 나타났다.

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느타리버섯과 사철느타리버섯의 균주간(菌株間) 및 종간(種間) 교배(交配) (Intra- and Intermatings among Strains of Pleurotus ostreatus and P. florida)

  • 고승주;차동열;박영환
    • 한국균학회지
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    • 제9권1호
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    • pp.13-18
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    • 1981
  • 1. 느타리버섯 농기(農技) 201호(號), 기존보급품종(旣存普及品種), 사철느타리버섯은 사극성(四極性) 교배형식(交配形式)을 가지고 있으며 각(各) 균주간(菌株間)에 는 교배(交配)가 가능(可能)하였다. 2. 균주간(菌株間) 교배균주(交配菌株)는 균주내(街株內) 교배종주(交配種株)보다 균사생장(菌絲生長)이 빠르고 자실체(子實體) 수량(收量)도 높았다. 3. 다수성(多收性)인 느타러버섯 농기(農技) 201호(號) 및 사철느타리버섯의 1핵(核) 균사체(菌絲體)를 교배(交配)할 때 자실체(子寶體) 수량(收量)이 가장 높았다.

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구름버섯의 균사배양액 및 자실체로부터 얻어진 단백다당체의 수율비교 (Comparisons of Protein-bound Polysaccharide Contents Obtained from Mycelial Cultured Broth and Fruit body of Coriolus versieolor)

  • 박영도;홍용기;황완균;허재두;박신
    • 한국균학회지
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    • 제17권4호
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    • pp.223-228
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    • 1989
  • 구름버섯의 균사체배양액과 자실체로부터 높은수율의 단백다당체를 얻기 위하여 균주 및 배지별에 따른 차이와 폐기물을 이용한 균배양에 대하여 실험을 실시한 결과를 요약하면 다음과 같다. 계통이 다른 4종의 균주 중에서 16002 균주가 0.58%로 가장 높은 수율을 보였으며 나머지 3균주 간에는 큰 차이가 없었다. 평판배지상에서 균사의 생장이 양호했던 배지들 중 액내 배양하여 단백다당체를 추출한 결과 CVT-III가 0.51%로 가장 양호했으며 각 배지간에는 큰 차이를 보였다. 약재추출박과 구름버섯의 자실체 추출박을 배지의 성분으로 이용했을 때 구름버섯의 추출박을 이용한 배지에서는 대조군에 비해 대등한 수율을 나타냈으며 인삼추출박을 첨가했을 때는 11.4-48%의 수율증가를 보였다. 인공재배를 한 자실체간에 있어 단백다당체의 수율을 비교했을 때 균사배양액에서와 같은 경향을 보였으며 높은 수율의 단백다당체를 얻기 위해서는 어린시기에 자실체를 수확하는 것이 효과적이었다.

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