• Title/Summary/Keyword: high-pressure extract method

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Quality Characteristics of Fish, Crab and Red-Crab Stock Prepared by High Pressure Extract Method (고압 가열 추출 방식을 이용한 생선 육수, 붉은 대게 육수, 대게 육수의 품질 특성)

  • Bae, Gum-Kwang;Byun, Gwang-In;Cho, Soo-Keun
    • Culinary science and hospitality research
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    • v.13 no.4
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    • pp.293-304
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    • 2007
  • Using the high-pressure heating extraction process suitable for mass-production, crab stock(CS), red crab stock(RCS) and fish stock(FS) were manufactured and their quality and organic features were compared. The results are as follows. In mineral content, the content of essential amino acids, the content of delicious amino acids and preference, CS and RCS were rated higher than FS, and especially RCS had the most abundant essential amino acid content and had the highest rating in organic evaluation. Also, in terms of cost, RCS was more economical than regular CS, which is relatively expensive. Therefore, it is believed that replacing fish bones with RCS in making stock will be more cost-saving and have higher preference.

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The Analysis of Physicochemical and Sensory Characteristics in Brown Stock - Comparison of Traditional Method and High-Pressure Extracted Method - (갈색 육수의 이화학적 및 관능적 특성 분석 - 전통 방식과 고압 가열 방식 비교 -)

  • Choi, Soo-Keun;Jang, Hyuk-Rae;Rha, Young-Ah
    • Culinary science and hospitality research
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    • v.14 no.3
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    • pp.196-209
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    • 2008
  • This study was conducted to mass-produce brown stock optimized by using a high-pressure heating extractor and to use brown stock as a material for developing various products. For these purposes, we attempted to produce standardized brown stock by extracting brown stock using a high-pressure heating extractor and compared it with brown stock extracted by the traditional method in terms of general elements and mechanical and sensory characteristics. With regard to how to prepare optimal brown stock, the best brown stock was that extracted seven times repeatedly by the traditional method, but the method had a large economic loss in terms of material consumption and took a long time in extraction. Thus, considering time and labor, it was concluded that extraction at 120$^{\circ}C$ for 15 hours using a high-pressure heating extractor is the optimal extraction condition in terms of economic efficiency and quality. The results of this study are expected to be useful as a practical material for making brown stock production process more convenient, applying cooks' traditional cooking techniques to mass production, maintaining standardized superior quality and taste, and improving shelf life.

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Extract of high hydrostatic pressure-treated danshen (Salvia miltiorrhiza) ameliorates atherosclerosis via autophagy induction

  • Ko, Minjeong;Oh, Goo Taeg;Park, Jiyong;Kwon, Ho Jeong
    • BMB Reports
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    • v.53 no.12
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    • pp.652-657
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    • 2020
  • Danshen (Salvia miltiorrhiza) is a traditional medicinal plant widely used in Asian countries for its pharmacological activities (e.g., amelioration of cardiovascular diseases). In this study, we investigated the anti-atherosclerotic activity of raw danshen root extract prepared using high hydrostatic pressure (HHP) at 550 MPa for 5 min and hot water extraction. This method was useful for elimination of bacteria from cultured danshen plants and for better extraction yield of active principles. The HHP-treated danshen extract (HDE) inhibited proliferation of human umbilical vein endothelial cells (HUVECs) and induced autophagy that was assessed by LC3 conversion and p62 degradation. HDE suppressed foam cell formation in oxLDL-induced RAW264.7 macrophages; lysosomal activity simultaneously increased, measured by acridine orange staining. HDE also reduced atherosclerotic plaque development in vivo in apolipoprotein E knock-out (ApoE-/-) mice fed a high cholesterol diet. Taken together, these results indicated that HDE exhibited anti-atherosclerotic activity via autophagy induction.

Effects of Ethanol Extract of the Rhodiola Sachalinensis for Anti-oxidation and Blood Pressure and Skeletal Muscles Contractility in Rat (고산흥경천 에탄올 추출물의 항산화작용과 쥐의 혈압 및 골격근 수축성에 미치는 영향)

  • Jin, Yinzhe;Cheng, Yu-dong;Lee, Kwang-Jin;Jung, Yong-An;Row, Kyung-Ho
    • KSBB Journal
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    • v.26 no.3
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    • pp.211-216
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    • 2011
  • To study the effects of ethanol extract of the Rhodiola Sachalinensis on the anti-oxidation and blood pressure and skeletal muscles contractility in rats. The ethanol extracts and fractions of Rhodiola Sachalinensis were determinated the anti-oxidation effects by DPPH(1,1-dipenyl-2-picrylhydrazyl) method and comparing with the BHA (Butylated hydroxyanisole) and AA(Ascorbic acid). The gastrocnemius and soleus muscle contractility were observed by stimulating the sciatic nerve with electricity after affusing stomach with ethanol extract of the 200 mg/kg dosage of Rhodiola Sachalinensis for 4 weeks. The ethanol extract of the Rhodiola Sachalinensis could shorten latent period, increase maximal contractive extent and time and relaxative time at the twitch and complete tetanus of gastrocnemius and soleus muscles. The ethanol extract of the Rhodiola Sachalinensis shows high anti-oxidation effect and can enhance skeletal muscles contractility in rats.

Enhancement of Skin Immune Activities of Spirulina maxima by High Pressure Extraction Process (Spirulina maxima 초고압 추출물의 피부 면역 활성 증진)

  • Oh, Sung-Ho;Kang, Do-Hyung;Choi, Woon-Yong;Seo, Yong-Chang;Heo, Soo-Jin;Abu, Affan Md.;Jeong, Kyung-Hwan;Lee, Hyeon-Yong
    • Ocean and Polar Research
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    • v.32 no.2
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    • pp.157-164
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    • 2010
  • A marine alga, Spirulina maxima, was extracted under high pressure and low temperature conditions at 500 MPa and $60^{\circ}C$ for 5 and 10 min. A high pressure of 500 MPa was applied to improve process yields because of low temperature extraction. This method resulted in highest higher extraction yield of 26.1% (w/w) in comparison to those results obtained from conventional extraction methods which produced a yield of 17.6% (w/w) from water. The extracts from this process also showed 19% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. The crude extract significantly reduced the production of Prostaglandin $E_2$ ($PGE_2$) from CCD-986sk cells and increased nitric oxide production by macrophages. These higher activities of enhancing skin immune functions were found to have high antioxidant extract properties, like a 98% increase in DPPH radical scavenging activity. The extracts from the high pressure process showed a higher elution of active components than other processes and generated new compounds based on HPLC analysis. This clearly indicates that the extracts from high pressure and low temperature conditions have higher skin immune activation properties that have not been previously reported.

A Study on the Anti-microbial Effect on S. mutans and Anti-oxidant Effect of Zanthoxylum pericarpium Extract (산초 추출물의 S. mutans 항균활성 및 항산화 효능 연구)

  • Lee, Sang-Gon;Park, Chan-Ik
    • The Korea Journal of Herbology
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    • v.26 no.4
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    • pp.181-185
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    • 2011
  • Objectives : The Zanthoxylum pericarpium has been used as oriental spicy seasoning and a medicinal plant from old times. This study was performed to determine the anti-oxidant efficacy of Zanthoxylum pericarpium extract and the anti-microbial effects. Methods : We got Zanthoxylum pericarpium extract using PSE (pressurized solvent extraction) method. The anti-microbial effect of Zanthoxylum pericarpium extract was assessed on Streptococcus mutans(S. mutans) and anti-oxidant effect of the extract was assessed by measuring DPPH radical scavenging activity and SOD like activity. Results : 1. Zanthoxylum pericarpium extract had high anti-microbial activity on S. mutans. 2. DPPH radical scavenging activity significantly increased in the Zanthoxylum pericarpium extract. 3. SOD like activity also significantly increased in the Zanthoxylum pericarpium extract. Conclusions : The PSE extract from Zanthoxylum pericarpium has good anti-microbial and anti-oxidant effects in a concentration-dependent manner.

The Analysis of Minerals and Free Amino Acid in Brown Stockwith Extracted Methods Varied (추출방법을 달리한 브라운 스톡의 무기질 및 유리아미노산 분석)

  • Jang, Hyuk-Rae;Lee, Bo-Soon;Choi, Soo-Keun
    • Culinary science and hospitality research
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    • v.14 no.3
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    • pp.210-222
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    • 2008
  • This study showed that the brown stock, which is the base of demi-glace sauce, extracted by using a high pressure heating extractor is more advantageous than that extracted by the traditional extraction method for the mass production. We compared the former with the latter in terms of minerals and free amino acids. The results of this study are summarized as follows. When mineral contents were compared, the brown stock extracted by high.pressure heating extraction showed the tendency of increase in mineral contents in proportion to heating temperature and heating time, but, from extraction temperature of 140$^{\circ}C$, the contents of K, Mg, Na and P decreased with the increase of extraction time. In addition, mineral contents in the brown stock extracted by high-pressure heating extraction were generally lower than those in brown stock extracted by the traditional extraction method. This result was produced probably because materials were added repeatedly in the traditional method. Amino acids contents in brown stock according to the extraction methods were also examined. They increased with the increase in the number of extractions in the brown stock extracted by the traditional method, and those in the brown stock extracted using a high pressure heating extractor increased with the increase in heating temperature and extraction time. The results of this study are expected to be useful as a practical material for the mass production of brown stock products.

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Compositional differences of Bojungikgi-tang decoctions using pressurized or non-pressurized extraction methods with variable extraction times

  • Kim, Jung-Hoon;Seo, Chang-Seob;Kim, Seong-Sil;Shin, Hyeun-Kyoo
    • The Korea Journal of Herbology
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    • v.28 no.4
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    • pp.1-6
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    • 2013
  • Objectives : In other to determine the optimal extraction conditions, the various Bojungikgi-tang (BJIGT) decoctions prepared by different pressure levels and different extraction times were compared and evaluated in terms of the extract yield and the total soluble solid content. Methods : Decoctions were prepared by the pressure levels of 0 (non-pressurized) and 1 $kgf/cm^2$ (pressurized) for 60, 120 and 180 min. The extract yield and the total soluble solids content of decoctions were measured, and the amounts of the reference compounds in decoctions were investigated by the analysis using high performance liquid chromatography. Results : The extract yield and the total soluble solid content were higher in decoctions extracted by the pressurized method than those from decoction with non-pressurized method. The patterns of yield and contents showed a proportional increase to the extraction time. In analysis of the linear regression for four reference compounds such as liquiritin, nodakenin, hesperidin, and glycyrrhizin, the good linearity with the correlation coefficient more than 0.9999 was observed. The highest contents for four reference compounds were observed at 180 min of both the pressurized method and the non-pressurized method. Conclusions : This study suggests that the pressure in extraction method and the extraction time affect the compositional constituents in BJIGT decoctions. The extraction time of 180 min could be chosen in both pressurized and non-pressurized method as optimal extraction condition.

Rapid Isolation of Cyanidin 3-Glucoside and Peonidin 3-Glucoside from Black Rice (Oryza sativa) Using High-Performance Countercurrent Chromatography and Reversed-Phase Column Chromatography

  • Jeon, Heejin;Choi, Janggyoo;Choi, Soo-Jung;Lee, Chang Uk;Yoon, Shin Hee;Kim, Jinwoong;Yoon, Kee Dong
    • Natural Product Sciences
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    • v.21 no.1
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    • pp.30-33
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    • 2015
  • Anthocyanins are water soluble plant pigments which are responsible for the blue, red, pink, violet colors in several plant organs such as flowers, fruits, leaves and roots. In recent years, anthocyanin-rich foods have been favored as dietary supplements and health care products due to diverse biological activities of anthocyanins including antioxidant, anti-allergic, anti-diabetic, anti-microbial, anti-cancer and preventing cardiovascular disease. High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase medium pressure liquid chromatography (RP MPLC) method was applied for the rapid and efficient isolation of cyanidin 3-glucoside (C3G) and peonidin 3-glucoside (P3G) from black rice (Oryza sativa L., Poaceae). The crude black rice extract (500 mg) was subjected to HPCCC using two-phase solvent system composed of tert-butyl methyl ether/n-butanol/ acetonitrile/0.01% trifluoroacetic acid (TBME/B/A/0.01% TFA, 1 : 3 : 1 : 5, v/v, flow rate - 4.5 mL/min, reversed phase mode) to give enriched anthocyanin extract (37.4 mg), and enriched anthocyanin extract was sequentially chromatographed on RP-MPLC to yield C3G (16.5 mg) and P3G (8.7 mg). The recovery rate and purity of isolated C3G were 76.0% and 98.2%, respectively, and those of P3G were 58.3% and 96.3%, respectively. The present study indicates that HPCCC coupled with RP-MPLC method is more rapid and efficient than multi-step conventional column chromatography for the separation of anthocyanins.

Optimized Analytical Method of Ether Extract in Leaf Tobacco by XT15(ANCOM) (AOCS Method) (XT15(ANCOM)를 활용한 잎담배 에테르 추출물의 최적 분석방법 구명(AOCS Method))

  • Na, Seung-Ju;Lee, Jung-Lae;Ha, Ah-Rang;Kim, Suk-Jae;Seo, Jung-Kyu
    • Journal of the Korean Society of Tobacco Science
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    • v.32 no.2
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    • pp.88-94
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    • 2010
  • The ether extract(EE) of leaf tobacco contains diverse compounds such as lipid, resinoid, paraffin hydrocarbons and pigments. Although the correlation of EE concentertion with leaf tobacco has not been established clearly, it is known that in some concentration range, EE concentration of leaf tobacco exerts good influence on the quality of tobacco. Recently, The American Oil Chemist's Society introduced new method(Am 5-04; AOCS, 2009) to determine EE concentration by AT15 extractor. This method is based on extraction with organic solvent at relatively high temperature and pressure, which significantly reduces the extraction time and ensures safety. The aim of this study is to optimize analytical condition of AOCS method for analyzing EE concentration of leaf tobacco. When sample pre-drying time and extraction time of XT15 were set to 3 hour and 30 minute and 30 minute respectively, EE concentration obtained from AOAC method. Statistical analysis(T-test) showed that there is no difference(P>0.05) between EE concentrations from two methods.