Optimized Analytical Method of Ether Extract in Leaf Tobacco by XT15(ANCOM) (AOCS Method)

XT15(ANCOM)를 활용한 잎담배 에테르 추출물의 최적 분석방법 구명(AOCS Method)

  • Received : 2010.11.08
  • Accepted : 2010.11.29
  • Published : 2010.12.24

Abstract

The ether extract(EE) of leaf tobacco contains diverse compounds such as lipid, resinoid, paraffin hydrocarbons and pigments. Although the correlation of EE concentertion with leaf tobacco has not been established clearly, it is known that in some concentration range, EE concentration of leaf tobacco exerts good influence on the quality of tobacco. Recently, The American Oil Chemist's Society introduced new method(Am 5-04; AOCS, 2009) to determine EE concentration by AT15 extractor. This method is based on extraction with organic solvent at relatively high temperature and pressure, which significantly reduces the extraction time and ensures safety. The aim of this study is to optimize analytical condition of AOCS method for analyzing EE concentration of leaf tobacco. When sample pre-drying time and extraction time of XT15 were set to 3 hour and 30 minute and 30 minute respectively, EE concentration obtained from AOAC method. Statistical analysis(T-test) showed that there is no difference(P>0.05) between EE concentrations from two methods.

Keywords

References

  1. AOAC (2000) Official methods of analysis 17th Ed., Gaithersburg, MD, official Method 991:36, 2004.
  2. AOCS (2004) Rapid determination of oil/fat utilizing high temperature solvent extraction, Aocs Approved Procedure Am 5-04
  3. Palmquist, D. L. and Jenkins, T. C. (2003) Challenges with fats and fatty acid methods, J. Anim. Sci., 81: 3250-3254. https://doi.org/10.2527/2003.81123250x
  4. Thiex, N. J., Anderson, S. and Gildemeister, B. (2003) Crude fat, diethyl ether extraction, in feed, cereal grain, and forage (Randall/Soxtec/Submersion Method) : Collaborative Study. J. AOAC Internation, 86(5), : 888-898.
  5. Silva, P. T., Detmann, E., ValadaresFilho, S. C., Detmann, K. S. C., Barros, L. V., Martins, S. C. V., Morais, L. E. and Costa, V. A. C. (2011) Evaluation of total and non-fatty ether extract in feeds and cattle feces using two analytical methods, Anim. Feed Sci. Technol. 163(2): 111-117. https://doi.org/10.1016/j.anifeedsci.2010.10.012
  6. Tso, T.C. (1990) Production, physiology and biochemistry of tobacco plant. institute of international development & education in agricultural and life sciences, USA. 610.
  7. 한국인삼연초연구소 (1991) 담배성분분석법, 146-148.
  8. 한국연초학회 (1997) 담배 연구의 최근동향, 14-15.