• 드라이브 ㆍ 컨트롤
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• 제21권2호
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• pp.30-35
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• 2024

Domestic rice is more expensive than imported products, so it is necessary to reduce production costs to secure competitiveness. Low-density planting developed in Japan is a cultivation technology that reduces labor and production costs without yield loss. The area of low-density cultivation is continuously increasing. However, research on how rice transplanters adapt to low-density planting has not been conducted. Therefore, this study was carried out to determine the optimal working conditions of a rice transplanter for low-density planting. Three types of rice transplanters were used and treated based on 3 conveying distance levels. The number of picked seedlings, pick missing rate, the number of planted seedlings, and the mis-planted rate were investigated to evaluate planting accuracy according to the transfer distance to the seedling tray. The results showed that the number of planted seedlings was 4.31~4.95 EA with an L1 seedling tray transfer distance (horizontal 9 mm, vertical 8 mm), but the mis-planted rate was higher than in other conditions. At L2 (horizontal 9 mm, vertical 10 mm) and L3 (horizontal 11 mm, vertical 8 mm) transfer distance conditions, the number of planted seedlings were 4.89-5.68 EA and 4.69-5.66 EA, respectively, with a low mis-planted rate of less than 3%. The results showed that if the transfer distance is adjusted properly, a rice transplanter can be used for low-density planting with high planting accuracy.

• 현장농수산연구지
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• 제21권2호
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• pp.77-86
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• 2019

To determine none top soil covering in rice seedling nursery method for the sparse machine transplanting, four different sowing methods were tested. Shoot and root length, fresh weight, leaf number and color using leaf color chart(LCC) and SPAD were collected as the data comparison of methods. The seedling height showed the highest growth according to the conventional (230g seed rate of pre-emerged seeds and top-soil covering) > high sowing density 1 (290g seed rate of pre-emerged seeds and top-soil covering) ≥ high sowing density 2(290g seed rate of pre-emerged seeds and none top-soil covering) > high sowing density 3(290g seed rate of iron-coated seeds and none top-soil covering). There was any statistical difference between groups in root length, leaf number, LCC, and SPAD values. Thus, a high sowing density of 290g for rice nursery seedling box was recommended to the sparse machine transplanting in rice cultivation with the none top-soil covering method, enabling convenient handling in transportation and machine transplanting work.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.845-848
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• 2001

For understanding physiology and metabolism under various culture conditions, combined analysis of transcriptome and proteome is attractable way. We have manufactured DNA microarray containing 2,850 genes including all functionally known and putative ones. In this study, we report analysis of transcriptome and proteome during the high cell density culture of E. coli by using DNA microarray and 2-DE. Fed-batch fermentation of E. coli was carried out by exponential feeding of nutrients until the maximum cell density reached 74 g dry cell weight/L (g DCW/L). Changes in transcriptome and proteome during the HCDC are analyzed qualitatively and quantitatively to provide their physiological and metabolic meanings.

• Biotechnology and Bioprocess Engineering:BBE
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• 제1권1호
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• pp.51-53
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• 1996

Fed-batch culture of Pseudomonas oleovorans was carried out for the production of medium-chain-length polyhydroxyalkanoates (MCL-PHAs) using octanoate as a carbon source. Octanoate and the salt solution containing ammounium sulfate and magnesium sulfate were intermittently fed in the course of fermentation. Cell mass and PHA concentrations of 42.8 and 16.8g/L, respectively, could be obtained in 40 h. The PHA content and the PHA productivity were 39.2% and 0.42 g PHA/L-h, respectively. The yields of cell mass and PHA were 0.71 g dry cell mass/g octanoate and 0.28g PHA/g octanoate, respectively. Therefore, octanoate can be used for the production of MCL-PHAs to a high concentration with high productivity.

• ALGAE
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• 제35권3호
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• pp.277-292
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• 2020

Omega-3 fatty acids, including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are polyunsaturated fatty acids beneficial to human health. A limited number of microalgae have been used for commercial omega-3 production, which necessitates the identification of new microalgae with high omega-3 contents. We explored the fatty acid composition and EPA and DHA contents of the mixotrophic dinoflagellate Gymnodinium smaydae fed with the optimal algal prey species Heterocapsa rotundata. Cells of G. smaydae were found to be rich in omega-3 fatty acids. In particular, the DHA content of G. smaydae was 21 mg g^-1 dry weight, accounting for 43% of the total fatty acid content. The percentage of DHA in the total fatty acid content of G. smaydae was the highest among the reported microalgae except for Crypthecodinium cohnii. Moreover, to determine if the prey supply interval affected the growth rate of G. smaydae and its fatty acid content, three different prey supply intervals (daily, once every 2 d, and once for 4 d) were tested. Daily prey supply yielded the highest total fatty acid and DHA contents in G. smaydae. Furthermore, we successfully produced high-density G. smaydae cultures semi-continuously for 43 d with daily prey supply. During the semi-continuous cultivation period, the highest density of G. smaydae was 57,000 cells mL^-1, with an average growth rate of 0.7 d^-1. Taken together, the percentage of EPA and DHA in the total fatty acid content was maintained in the range of 54.2-56.9%. The results of this study support G. smaydae as a promising microalgal candidate for commercial DHA production and demonstrate that daily supply of prey can efficiently produce high-density G. smaydae cultures for more than a month.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2000년도 춘계학술대회 학술발표 논문집
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• pp.59-62
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• 2000

In cope with insufficient agricultural labor and requirement of high quality product Hydroponics is a really good method. It makes the high density agriculture possible and all the growing environments controllable. So its research is so much progressing to maximize the quantity and quality of farm products. Furthermore, the big progress, in the research of a future agriculture, is systematically conducted for the automatic controlled system. In this paper, a new approach to the automation of the cultivation in a green house is suggested and a practical automatic control cultivation system is implemented. To automatically control and optimize the very nonlinear and time-varying growth of farm products, a hybrid strategy(FECA; Fuzzy Expertized Control Algorithm) is proposed which serially combines a fuzzy expert system with the fuzzy logic control. The fuzzy expert system(FMES; Fuzzy Model-based Expert System) is intended to overcome the non-linearity of the growth of farm products. The part of fuzzy controller is incorporated to solve the time-variance of the growth of farm products. Finally, the efficiency and the effectiveness of the implemented agricultural automation system is presented through the cultiviation results.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1603-1611
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• 2009

High-cell-density cultivation of yeast was investigated using the agricultural waste products corn steep liquor (CSL) and molasses. The Saccharomyces cerevisiae KV-25 cell mass was significantly dependent on the ratio between C and N sources. The concentrations of molasses and CSL in the culture medium were statistically optimized at 10.25% (v/v) and 16.87% (v/v), respectively, by response surface methodology (RSM). Batch culture in a 5-l stirred tank reactor using the optimized medium resulted in a cell mass production of 36.5 g/l. In the fed-batch culture, the feed phase was preceded by a batch phase using the optimized medium, and a very high dried-cell-mass yield of 187.63 g/l was successfully attained by feeding a mixture of 20% (v/v) molasses and 80% (v/v) CSL at a rate of 22 ml/h. In this system, the production of cell mass depended mainly on the agitation speed, the composition of the feed medium, and the glucose level in the medium, but only slightly on the aeration rate.

• 한국육종학회지
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• 제41권4호
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• pp.556-559
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• 2009

"홍소"는 국립원예특작과학원에서 1989년 "양광"에 "홍로"를 인공교배 하여 얻은 실생 중 유망한 계통을 2002년 1차 선발하여 2003년부터 2006년까지 4년간 '원교 가-30호'로 군위, 춘천 등 5개 지역에서 적응시험을 거쳐 2006년에 최종 선발하였다. 과실의 적숙기는 9월 상순으로 "홍로"와 비슷하다. 과형은 원원추형, 과피색은 선홍색, 바탕색은 녹황색, 과육색은 백색이다. 과실크기는 295 g으로 중 대과종이며 가용성고형물 함량은 $14.3^{\circ}Brix$, 산 함량은 0.34%로 "홍로"에 비해 산도가 높으며 당산미가 조화되어 식미가 우수하였다. 수자는 반개장성이고 수세는 약한 편이고, 조기결실성이며 새가지 생장이 적고 단과지형이나 액화 결실이 적어서 생력형 밀식재배 품종으로 유망하였다. 점무늬낙엽병에는 강하고 동녹 발생이 비교적 많은 편이며, 특히, 주요 재배품종과의 교배 친화성이 높다.

• 한국토양비료학회지
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• 제41권5호
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• pp.279-284
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• 2008

화산회토 감귤원에서 제초제를 사용하는 청경재배, 자연초종을 이용하는 자연초생재배, 켄터키블루그라스를 이용하는 인위초생재배를 4년간 동일한 방법으로 토양 및 시비관리를 하면서 토양의 물리성과 미생물체량, 토양인산효소 활성을 분석하였다. 자연초생재배가 청경재배보다 입단계수(>0.5 mm)는 26.7% 높았고 토양경도는 11.8 mm 낮았다. 토양의 용적밀도와 공극율은 차이가 없었다. 초생재배가 청경재배보다 인산효소 활성이 높았으며 토양 미생물체량은 인위초생재배가 $525.4mg\;kg^{-1}$로 청경재배보다 2배 이상 높았다. 결론적으로 초생재배는 입단형성이 잘되고 토양 경도가 개선되는 효과가 있었으며 토양 미생물활성이 높았다. 비화산회토 감귤농가 중 5년 이상 된 제초제를 이용하는 관행재배, 자연 초생재배 저농약, 유기농 인증 재배 감귤원 5개를 선정하여 감귤나무의 개화기인 5월에 토양을 채취 후 인지질 지방산과 토양인산효소 활성, 미생물체량을 분석하였다. 인지질지방산함량은 유기재배 감귤원이 $112.2n\;mol\;g^{-1}$로 관행재배보다 2배 이상 많았다. 인지질 지방산의 미생물지표에 따른 유기재배토양의 그램 음성세균은 15.1%, 방선균은 6.6%로 관행재배토양 보다 높았다. 유기재배 감귤원이 관행재배보다 토양 미생물체량은1.5배 정도 많았고 토양인산효소 활성은 17.6% 높았다.

• 한국산림과학회지
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• 제101권1호
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• pp.77-82
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• 2012

표고를 재배하기 위하여 톱밥배지를 $65^{\circ}C$로 저온살균하는 과정은 살균시설비용이 적게들고 배지성분도 보전되는 장점이 있으나, 오염 확률이 높아서 효과는 일정하지가 않았다. 본 연구는 참나무 톱밥배지의 저온살균 후 성공률이 높은 효과적인 배양조건을 알고자 하였다. 참나무톱밥배지를 $65^{\circ}C$, $100^{\circ}C$, $121^{\circ}C$로 살균한 후, 표고톱밥종균을 접종하고 $15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$에서 배양하거나, 표고종균의 접종간격을 1 cm, 2 cm, 4 cm(표면적 비율은 4%, 11%, 25%)로 하여 표고균사의 생장과 배지의 오염정도를 비교하였다. 저온살균된 배지에서 표고균사생장은 배양온도 $25^{\circ}C$ 이하에서는 $100^{\circ}C$나 $121^{\circ}C$ 살균된 배지에서 처럼 양호하였으나, $30^{\circ}C$ 배양에서는 표고균사생장이 크게 위축되었다. 그리고 저온살균배지에서 표고종균 접종밀도를 1 cm 간격으로 하였을 때에는 배지가 표고균사로 완전점유되었으나, 2~4cm 간격 접종밀도에서는 14~33%의 배지가 오염되었다. 따라서 저온살균배지는 $25^{\circ}C$ 이하에서 25% 이상의 충분한 종균을 접종하여 배양하는 것이 성공적이라고 결론짓는다.