• Title/Summary/Keyword: high salinity

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Characterization of Oszinc626, knock-out in zinc finger RING-H2 protein gene, in Ac/Ds mutant lines of rice(Oryza sativar L.) (Zinc finger RING-H2 protein관련 Ac/Ds전이인자 삽입 변이체 Oszinc626 유전자의 특성 분석)

  • Park, Seul-Ah;Jung, Yu-Jin;Ahn, Byung-Ohg;Yun, Doh-Won;Ji, Hyeon-So;Park, Yong-Hwan;Eun, Moo-Young;Suh, Seok-Cheol;Lee, Soon-Youl;Lee, Myung-Chul
    • Journal of Plant Biotechnology
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    • v.35 no.3
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    • pp.177-183
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    • 2008
  • Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless(Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626(NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2(C3-H2-C3 type) domain for this group of protein is $Cys-X_2-Cys-X_{28}-Cys-X-His-X_2-His-X_2-Cys-X_{14}-Cys-X_2-Cys$. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.

Study on the Salt and Sodium Content of Middle School Lunch Meals in Gyeongsangbuk-do Area - Focus on Application of 'SamSam Foodservice' - (경북 일부지역 중학교 점심급식의 소금 및 나트륨 함량 분석 - 삼삼급식소 적용을 위한 기초조사를 중심으로 -)

  • Park, So-Young;Lee, Kyung-A
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.5
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    • pp.757-764
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    • 2016
  • The purpose of this study was to investigate the salt and sodium content of lunch meals served at middle schools in Gyeongsangbuk-do. Four middle schools were investigated for their salt and sodium content and salt content contributing rate per meal. Average salt content of every lunch meal was 4.41 g, and average sodium content was 1,736.02 mg. During the investigation period, the lowest sodium content was 943.19 mg while the highest was 2,827.56 mg. Samples were classified into 16 food items and investigated for salt and sodium content. Total average salinity was 1.06%. Kimchi was the saltiest, followed by fresh-vegetables, boiled-vegetables, stir-fried foods, pan-fried foods, and hard-boiled foods. Total average salt content was 0.74 g, and most salty dishes were single dish rice noodles, followed by stews, steamed foods, broths, soups, kimchies, stir-fried foods, roasted foods, and hard-boiled foods. Samples were classified into seven menu groups, including cooked rice, single dish rice noodles, soup stew, main dish, side dish, kimchi, and desserts. Contributing rate of total average salt content was high in single dish rice noodles (40.56%), soup stew (23.23%), kimchies (20.30%), and main dish (18.13%). These results can be useful for establishing a database for sodium contents of meals in middle schools. 'SamSam foodservice' should be initiated in school foodservice to reduce sodium intake.

Characteristic Analysis and Production of Short-Ripened Korean Traditional Soy Sauce Added with Rice Bran (미강 첨가량에 따른 단기숙성 간장의 제조 및 특성 분석)

  • Jeong, Su-Ji;Shin, Mee-Jin;Jeong, Seong-Yeop;Yang, Hee-Jong;Jeong, Do-Youn
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.550-556
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    • 2014
  • Rice bran contains both excellent nutritional value and functional advantages. Its utilization is limited due to reducing texture and low storage. To satisfy various tastes, Bacillus spp. having high amylase and protease activities were selected. Using the strains, we made whole grain soybean Meju with a reduced manufacturing period by increasing the concentration of total nitrogen. We made soy sauces with mashing ratios of soy bean and rice bran at 10:0, 9:1, 7:3, and 5:5, and then compared their physiochemical properties. After 2 weeks of fermentation, the sugar content increased from 21~22% to 30~32%. However, pH and salinity showed no differences. At a ratio of 9:1, total nitrogen, amino nitrogen content, and total free amino acid contents were the highest at 1.62%, 652.52 mg%, and 8,804.03 mg/kg, respectively, compared to other mashing ratios of soy bean and rice bran. Especially, the contents of aspartic and glutamic acid, which increase delicate flavoring, were higher in our soy sauce compared to those of general traditional soy sauce and brewed soy sauce, which were 504.25 and 1,262.25 mg/kg, respectively. Serine and alanine, which are related to sweet taste, were present at 49.50 and 518.75 mg/kg, respectively, which were the highest among all mixing ratios, at a ratio of 9:1. Compared to general traditional soy sauce and brewed soy sauce, the contents of histamine and tyramine among biogenic amines decreased to 35.85 and 41.04 mg/kg, respectively. Finally, a soy bean and rice bran mixing ratio of 9:1 was determined to be the optimal mixing ratio in the sensory evaluation.

Gold-Silver Mineralization of the Geojae Area (거제(巨濟)지역 금(金)-은(銀)광상의 광화작용(鑛化作用) 연구)

  • Choi, Seon-Gyu;Chi, Se-Jung;Yun, Seong-Taek;Koh, Yong-Kwon;Yu, Jae-Shin
    • Economic and Environmental Geology
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    • v.22 no.4
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    • pp.303-314
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    • 1989
  • The electrum-silver-sulfide mineralization of the Geojae island area was deposited in three stages (I, II, and carbonate) of quartz and calcite veins that crosscut Late Cretaceous volcanic rocks and granodiorite(83 m.y.). Stages I and II were terminated by the onset of fractunng and breCCIation events. Fluid inclusion data suggest that the gold-sulfide-bearing stages I and II each evolved from an initial high temperature( near $370^{\circ}C$) to a later low temperature(near $200^{\circ}C$). Each of those stages represented a separate mineralizing system which cooled prior to the onset of the next stage. The relationship between homogenization temperature and salinity in stages I and II suggests a complex history of boiling, cooling and dilution. Evidence of boiling indicates a pressure of < 100 bars, corresponding to a depth of 500 to 1,250m assummg hthostatlc and hydrostatic pressure regimes, respectively. Fluid inclusion and mineralogical evidence suggest that the electrum-silver mineralization was deposited at a temperature of $220-260^{\circ}C$ from ore fluids with salinities between 1.9 and 8.1 equivalent wt.% NaCl. Total sulfur concentration is estimated to be $10^{-3}$ to $10^{-4}$ molal. The estimated $fs_2$ and $fo_2$ range from $10^{-11.8}$ to $10^{-14}$ atm and $10^{-35}$ to $10^{-36}$ atm, respectively. The chemical conditions indicate that the dominant sulfur species in the ore forming fluids was a reduced form($H_2S$). Rapid cooling and dilution of ore-forming fluids by mixing with less-evolved meteoric waters led to gold-silver deposition through the breakdown of the bisulfide complex($Au(HS)_2$) as the activity of $H_2S$ decreased.

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Studies on the Forming Mechanism of The Fishing Ground of Yellow tail, seriola quinqueradiata, in The Adjacent Sea of Jeju Island (제주도 주변해역의 방어어장형성기구에 관한 연구)

  • 김준택;노홍길;김상현
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.38 no.1
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    • pp.20-35
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    • 2002
  • We studied the fishing ground of a yellowtail and oceanographic conditions around Chujado and Marado around Jeju Island from autumn to winter. Also we investigate the forming mechanism of fishing ground around Marado, which shows the best catch of yellowtail near Jeju island. The obtained results are summarized as follow ; 1. When the high temperature water about 15.0∼19.0 in depth of 50m distributed around Chujado and Marado, the fishing ground of Yellowtail is shows a good catch. On the contrary, when the low temperature water about 11.0∼14.0 is shows a poor catch. 2. Fishing grounds of yellowtail by handline fishing are formed with a school of Yellowtail migrating southward from the coastal sea of Korea and slaying around Marado to winter and spawn due to following factors. Firstly, a front of temperature and salinity is formed between inshore water and oceanic water around Marado. Secondly, small-size eddies and excellent horizontal and vortical mix which is created by strong tide and geographical features including irregular underwater ground and an isolated island. An abundant school of yellow tails stays in front of the tide where a good fishing ground was made.

Fermentative Properties and Immunomodulating Activity of Low-sodium Kimchi Supplemented with Acanthopanax senticosus and Glycyrrhizae uralensis Extracts (가시오가피와 감초 추출물을 첨가한 저 나트륨 김치의 발효특성과 면역 활성능)

  • Yu, Kwang-Won;Suh, Hyung-Joo;Hwang, Jong-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.25 no.4
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    • pp.878-887
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    • 2012
  • This study investigated the fermentative characteristics and immunomodulating activity in Kimchi added with various salts (salt replacement and herb-salt with Acanthopanax senticosus and Glycyrrhizae uralensis) for the reduction of Na concentration in Kimchi. Kimchi using a salt replacement and herb-salt showed a higher level of acidity (0.8~0.84%) than that of the control (0.7%) at 7-day fermentation. Kimchi using a salt replacement and herb-salt showed a lower level of salinity (1.72~1.98%) than that of control (2.3~2.57%) during fermentation. The growth of Lactobacillus spp. and Leuconostoc spp. recorded the highest level ($2.3{\times}10^8$ and $2.8{\times}10^6cfu/g$, respectively) in control at 6 day-fermentation. However, those levels in Kimchi prepared with salt replacement and herb-salt were $3.5{\sim}5.4{\times}10^8$ and $6.1{\times}10^6cfu/g$, respectively. It is assumed that the high level of acidity of Kimchi prepared with salt replacement and herb-salt was caused by the increase in the growth of Lactobacillus spp. and Leuconostoc spp.. When the macrophage stimulating activity of salt replacement kimchi (Salt-R kimchi) supplemented with hot-water extract from Acanthopanax sentisus (AS) or Glycyrrhiza uralensis (GU) was investigated on aging period, Salt-RA kimchi with AS 5% at 6 days (2.78-fold of saline control at $100{\mu}g/m{\ell}$) and Salt-RG kimchi with GU 5% at 9 days (2.02-fold) significantly increased compared to the Salt-RA kimchi without AS or GU. In addition, Salt-RAG kimchi with AS 3% and GU 3% improved the bitter taste of Salt-RA and potently stimulated the macrophage at 6 days (1.28-fold of Salt-R kimchi) even though its activity was lower than Salt-RA (5%, 1.39-fold).

Extractive Nitrogenous Constituents in Commercial Tohajeot, a Salted and Fermented Freshwater Shrimp (Caridina denticulata denticulata), and their Quality Index (시판(市販) 토하(土蝦)젓의 함질소(含窒素) 엑스성분(成分) 조성(組成) 및 품질지표(品質指標)에 관(關)한 연구(硏究))

  • Park, Choon-Kyu;Park, Jung-Nim
    • Korean Journal of Food Science and Technology
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    • v.29 no.2
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    • pp.230-239
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    • 1997
  • In order to investigate the composition and the actual condition of extractive nitrogenous constituents in Tohajeot (a salted and fermented freshwater shrimp, Caridina denticulata denticulata) and in seasoned Tohajeot which were sold in the markets, the extract was analyzed separately into extractive nitrogen, free amino acids, oligopeptides, nucleotides and related compounds, quaternary ammonium bases, and guanidino compounds, using specimens collected at the fish markets of Yosu and Naju cities in 1994 and 1995. The salinity of Tohajeot was very high $(23.6{\sim}25.1)%$, but seasoned Tohajeot was relatively low $(8.4{\sim}11.4%)$. The extractive nitrogen in the extracts of Tohajeot and seasoned Tohajeot was $311{\sim}531\;mg\;and\;256{\sim}429\;mg$, and the total of free amino acids in them were $1,159{\sim}2,584\;mg\;and\;1,012{\sim}1,672\;mg$ respectively. Glutamic acid, leucine, lysine, histidine, alanine, ornithine, and tyrosine were the major amino acids in Tohajeot extract, and glutamic acid, lysine, arginine, aspartic acid, histidine, leucine and alanine were the main amino acids in seasoned Tohajeot. As for nucleotides and related compounds in them were $2.64{\sim}4.82\;{\mu}mol\;and\;1.08{\sim}1.93\;{\mu}mol$ respectively. Homarine, trigonelline, glycinebetaine and ${\beta}-alaninebetaine$ were detected in them. Homarine was the most abundant, ranging from 18 mg to 86 mg, but the others were very low. The content of major nitrogenous constituents in Tohajeot extract, such as extractive nitrogen, free amino acids, oligopeptides, nucleotides and related compounds, and betaines, was more abundant than that in seasoned Tohajeot extract. But the nitrogenous constituents of Tohajeot extract were poorer than those of anchovy sauce which was sold in the markets. Possibly, the extractive nitrogenous components, which consisted of total betains, total free amino acids, and phenylalanine might be recommended as the quality indices of standardizing Tohajeot and seasoned Tohajeot.

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Patterns of Leaching and Distribution of Cations in Reclaimed Soil according to Gypsum Incorporation Rate (석고 혼합량에 따른 간척지토양의 양이온 용탈 및 분포)

  • Ryu, Jin-Hee;Chung, Doug-Young;Hwang, Seon-Woong;Lee, Kyeng-Do;Lee, Sang-Bok;Choi, Weon-Young;Ha, Sang-Keun;Kim, Si-Ju
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.596-601
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    • 2010
  • Serious problems in reclaimed land for agriculture are high soil salinity and very poor vertical drainage. However, desalinization in these soils is very difficult. To identify the change of soil permeability by the gypsum incorporation in soils we observed elution patterns and salt distribution of the soil using soil columns packed with reclaimed saline soil with various rates of gypsum amendment. Saturated hydraulic conductivity ($K_{sat}$) of the top soil of reclaimed saline soils without gypsum incorporation was close 0 cm $hr^{-1}$ while $K_{sat}$ increased up to 0.3 cm $hr^{-1}$ with increasing amount of gypsum for 0.4% or more gypsum. Also $K_{sat}$ of the reclaimed saline soils for top soil was drastically increased to 1.0 cm $hr^{-1}$ or slightly greater after 141 hour's elution experiment. The cumulative amount of cation recovered in the effluent also increased in the order of gypsum incorporation rate 0.8% > 0.6% > 0.4 > 0.2%. Soil EC in soil columns decreased from initial 33.9 dS $m^{-1}$ to less than 0.4 dS $m^{-1}$ and exchangeable Ca 2+ increased by 32~140% according to gypsum incorporation rate.

Effects of Application of Solidified Sewage Sludge on the Growth of Bioenergy Crops in Reclaimed Land (간척지토양에서 하수슬러지 고화물 처리가 에너지작물의 생육에 미치는 영향)

  • An, Gi-Hong;Lee, Sun-Il;Koo, Bon-Cheol;Choi, Yong-Hwan;Moon, Youn-Ho;Cha, Young-Lok;Bark, Surn-Teh;Kim, Jung-Kon;Kim, Byung-Chul;Kim, Sang-Pyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.4
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    • pp.299-307
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    • 2011
  • This study was carried out to obtain the basic data for selecting the cultivatable bioenergy crops through application of solidified sewage sludge in reclaimed lands. The experimental plots consisted of the mixing with solidified sewage sludge plot (SS50), the covering with solidified sewage sludge plot (SS100), and the original reclaimed land plot (ORL) on reclaimed land for the intended landfill in Sudokwon Landfill Site Management Corporation (SLC). The growth of energy crops (Geodae-Uksae 1, Miscanthus sacchariflorus, and Phragmites australis) were investigated from May to October, 2010 in each experimental plot. The soil from ORL showed higher salinity with high contents of exchangeable $Na^+$ cation than that of SS50 and SS100. Soil properties on reclaimed land used in this study must be improved by increasing the buffering capacity of saline with the treatment of solidified sewage sludge due to the fact that the contents of organic matter (OM) in both of SS50 and SS100 were higher than that of the ORL. Thus the growth of energy crops cultivated in the solidified sewage sludge plots were better than in ORL. Geodae-Uksae 1 which showed an excellent adaptability on reclaimed land treated with the solidified sewage sludge has considerably higher biomass than those of other energy crops (M. sacchariflorus and P. australis). This study suggested that Geodae-Uksae 1 is the most suitable biomass feedstock crop for bioenergy productions, and the solidified sewage sludge may be possible to utilize as a soil cover materials for cultivation of bioenergy crops in reclaimed land.

The Early-Stage Changes of Water Qualities after the Saemangeum Sea-dike Construction (새만금 방조제 체절 이후 초기의 수질변화에 관한 연구)

  • Yang, Jae-Sam;Jeong, Yong-Hoon;Ji, Kwang-Hee;Kim, Hyun-Soo;Choi, Joeng-Hoon;Kim, Won-Jang
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.11 no.4
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    • pp.199-213
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    • 2008
  • Saemangeum salt-water Lake has been created by the completion of the sea-dike in April 2006. To monitor the water qualities of the lake during the sea-dike construction, salinity, SS, nutrients(DIN, DIP, DISi), and chlorophyll-$\alpha$ was analyzed for the surface water from 1999 to 2007. Due to the dike construction, weaker tidal current and lesser resuspension of bottom sediment resulted in the marked decrease of the concentrations of SS in the lake water. Consequently the clearer lake water has provided better condition for primary production with deeper penetration of sunlight into the water column and sufficient nutrient content in the water. Finally the chlorophyll-$\alpha$ content became approximately double in the concentration after the dike construction. Highly stimulated algal production with the marked decrease of the concentrations of SS was decreased the concentration of DIP in the surface water. On the other hand the concentration of DIN and DISi in surface water was increased after dike construction due to the expansion of the freshwater and the supply from bottom layer. As a result, the lake revealed an extremely high NIP ratio and a DIP-limited ecosystem. The lake has been transformed from a typical coastal ecosystem to a brackish one. Since the dike completion, the lake has shown a similar change pattern to the Geum River estuary. Due to the salt-wedge intrusion of seawater, it is highly probable to expect the formation of low-oxygen zone at the bottom layer near the river-mouth area of the lake during the summer. Therefore we need a continuous sentinel monitoring of bottom water qualities in the near future.

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