• Applied Biological Chemistry
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• v.47 no.2
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• pp.192-196
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• 2004

In order to measure cellular physiological activity including ion channel activity, protoplasts were isolated from the root tissue of tomato plant. The general methods recommended were not efficient enough to make protoplasts from the root tissue. Among various conditions tested, we found that a two-step treatment of osmosis is very efficient for the isolation of protoplasts. In this procedure, root tissues were preincubated in a solution containing 300 mM sorbitol for 30 min. Then, they moved to the reaction solution containing 700 mM sorbitol as well as cell wall-digesting enzymes. The formation of protoplast was greatly increased by this method. In order to find the optimal condition of the two-step method, various conditions of pH, osmotic pressure, incubation time, and the concentrations of cell wall-digesting enzymes were tested. The yield of protoplast isolation was maximal at pH 5.0 after 2 hr incubation. Mixed enzymes of 3% cellulase, 1 % macerozyme, and 0.1 % pectolyase showed maximal protoplast isolation. The physiological activity of isolated protoplast evaluated by measuring the cellular ATPase activity was as high as that measured from the preparation of root tissue. The protoplasts isolated by this method were remained healthy up to 4 hrs which is enough time to measure the cellular physiological activity. These results show that the two-step treatment of osmotic pressure was successful to obtain high yield of healthy protoplast from tomato root tissue.

• Journal of the Society of Disaster Information
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• v.14 no.4
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• pp.466-473
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• 2018

Purpose: Nowadays, it is the trend that seismic isolation method and combined method are used for seismic retrofitting, if concrete sphere foundation(CSF) system is applied to mid and low rise RC structure for the seismic isolation, the characteristics of concrete sphere, etc split tensile strength will be need. Method: The various experiments are carried out to know the split strength of high strength concrete sphere(60Mpa) and the ratio of split strength of concrete sphere to standard cylinder specimen, the size effect of concrete sphere with diameter. Results: It was purposed that the split strength of concrete sphere with diameter 150mm will be lower than that of cylinder specimen but, the average value is 4.39 Mpa and the ratio is higher than that of cylinder specimens, each 3.8% and 13.7%, the reason of this result is thought that the internal stress action of spot load and line load are different. Conclusion: There is a standard method for split tensile strength of cylinder type specimen, but there are few studies for the tensile split of concrete sphere. And therefore, in this study, theoretical and experimental details of concrete sphere will be served for the concrete sphere foundation or other sequent studies.

• Science of Emotion and Sensibility
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• v.26 no.4
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• pp.3-14
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• 2023

Recently, the effect of using social media on psychological well-being has been highlighted. However, studies exploring factors that may predict the quality of social media relationships are relatively rare. The present study investigated whether social media activity and psychological states, such as loneliness and depression, can predict the quality of social media relationships during the COVID-19 quarantine period using a machine learning technique. Ninety-five participants completed a self-report survey on loneliness, Instagram activity, quality of social media relationships, and depression at different time points (during the self-isolation and after the release of self-isolation). Similarity analyses, including multidimensional scaling (MDS), representational similarity analysis (RSA), and classification analyses, were conducted separately at each point in time. The results of MDS revealed that time spent on social media and depression were distinguished from others in the first dimension, and loneliness and passive use were distinguished from others in the second dimension. We divided the data into two groups based on the quality of social media relationships (high and low), and we conducted RSA on each group. Findings indicated an interaction between the quality of the social media relationships and the situation. Specifically, the effect of self-isolation on the high-quality social media relationship group is more pronounced than that on the low-quality group. The classification results also revealed that the predictors of social media relationships depend on whether or not they are isolated. Overall, the results of this study imply that social media relationship could be well predicted when people are not in isolated situations.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07a
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• pp.147-150
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• 2003

In this paper, We fabricated a high temperature pressure sensor using SBD(silicon- direct-bonding) wafer of $Si/SiO_2$/Si-sub structure. This sensor was very sensitive because the piezoresistor is fabricated by single crystal silicon of the first layer of SDB wafer. Also, it was possible to operate the sensor at high temperature over $120^{\circ}C$ which is the temperature limitation of general silicon sensor because the piezoresistor was dielectric isolation from silicon substrate using silicon dioxide of the second layer. The sensitivity of this sensor is very high as the measured result of D2200 shows $183.6\;{\mu}V/V{\cdot}kPa$. Also, the output characteristic of linearity was very good. This sensor was available at high temperature as $300^{\circ}C$.

• Journal of the Korean Society for Railway
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• v.5 no.3
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• pp.142-147
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• 2002

This paper proposes the Systematic Design Methodology of the Korean High Speed Train. High Speed Train does not operate in isolation and is a part of a rail system which is influenced through input and output effects from the environment and from the neighbouring system. To fullfil its overall desired function, such input and output relationships between the systems must be considered in the system boundary conditions. Therefore, the overall interrelationship of all these effects has to be carefully considered during the design process. Here proposed methodology may provide a guide line and criteria for the systematic problem solving method of that highly complex High Speed Train System.

• Journal of information and communication convergence engineering
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• v.1 no.3
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• pp.163-168
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• 2003

This paper presents a cascade multilevel PWM rectifier without the isolation transformers for energy build-up at each inverter modules. The features and advantages of the proposed PWM rectifier can be summarized as follows; I) It realizes the high power high voltage AC/DC power conversion, 2) It uses no transformer which is bulky and heavy, 3) It has hybrid structure so that switching devices can be effectively utilized, 4) It produces high quality AC current even in high power high voltage applications, 5) The input power factor remains unity by simple modulation index control. The multilevel rectifier is analyzed by using the circuit DQ transformation whereby the characteristics and control equations are obtained. Finally, it will be shown that the system simulation reveals the validity of analyses.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.6
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• pp.1026-1031
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• 2008

We report on the design and experimental results of a large diameter faraday rotator for the high-power laser system(KLF: Kaeri laser facility) that was completed in late 2007s at Korea Atomic Energy Research Institute. The design involves modelling the magnetic field of cylindrical coil with large diameter(110 mm). Magnetic field generation coil is designed by 6 layers using a rectangular wire with cross-sectional area $3{\times}5[mm^2]$. We obtain an isolation ratio for optical feedback of 35 dB at 1064 nm and magnetic field strengths ${\sim}25kG$. We expect that the design can be widely used optical isolators in high-power laser system.

• Proceedings of the KIPE Conference
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• 1997.07a
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• pp.393-399
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• 1997

Single-phase and three-phase AC to DC power converters are becoming frequently used for high voltage/high power applications such as telecommunications. They often require input/output transformer isolation for safety, a unity input power factor for minimum reactive power, free input harmonic currents fed back to the AC Power distribution system and, finally, high efficiency and high power density for minimum weight and volume. The proposed boost converter for power factor correction (PFC) provides an unity input power factor, low harmonic distortion and high efficiency along with reduced volume and weight. Single-phase 220VAC input/380VDC 1KW output prototype is constructed and experimental results will be verified with those of PSpice simulation.

• Proceedings of the KIPE Conference
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• 2001.10a
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• pp.576-580
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• 2001

This paper presents a casca multilevel PWM rectifier without the isolation transformers for energy build-up at each inverter modules; The features and advantages of the proposed PWM rectifier can be summarized as follows; 1) It realizes the high power high voltage AC/DC power conversion, 2) It uses no transformer which is bulky and heavy, 3) It has hybrid structure so that switching devices can be effectively utilized, 4) It produces high quality AC current even in high power high voltage applications, 5) The input power factor remains unity by simple modulation index control. The multilevel rectifier is analyzed by using the circuit DQ transformation whereby the characteristics and control equations are obtained. Finally, it will be shown that the system simulation reveals the validity of analyses

• Journal of Electrical Engineering and Technology
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• v.10 no.4
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• pp.1566-1577
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• 2015

A novel high step-up converter is presented herein, which combines the conventional buck-boost converter, the charge pump capacitor and the coupling inductor. By doing so, a quite high voltage conversion ratio due to not only the turns ratio but also the duty cycle, so as to increase design feasibility. It is noted that the denominator of the voltage conversion ratio is the square of one minus duty cycle. Above all, there is no voltage spike across the switch due to the leakage inductance and hence no passive or active snubber is needed, and furthermore, the used switch is driven without isolation and hence the gate driving circuit is relatively simple, thereby upgrading the industrial application capability of this converter. In this paper, the basic operating principles and the associated mathematical deductions are firstly described in detail, and finally some experimental results are provided to demonstrate the effectiveness of the proposed high step-up converter.