• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.3
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• pp.198-204
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• 2005

Seeds of neem were collected from different parts of India and analyzed for their azadirachtin content by High Performance Liquid Chromatography (HPLC). In order to assess the effects of genotypic and geographical variation on azadirachtin content in cell cultures, callus development was attempted in the seeds containing high and low concentration of azadirachtin. The concentration of azadirachtin in callus cultures was significantly affected by the explant source. Seed kernels with higher azadirachtin content produced higher azadirachtin content in callus cultures and lower azadirachtin content was seen in callus cultures produced from seed kernels with low azadirachtin content. The protocol for development of elite stock culture of Azadirachta indica was established with the objective of selecting a high azadirachtin-producing cell line. The highest azadirachtin-producing cell line was selected and the effects of different media and illumination conditions on growth and azadirachtin production were studied in shake flask suspension culture. Detailed batch growth kinetics was also established. These studies provided elite starter culture and associated protocols for cultivation of A. indica plant cell culture in the bioreactor.

• The Journal of the Korea Contents Association
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• v.21 no.7
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• pp.533-545
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• 2021

This study aimed to examine the differences in gender equality consciousness between latent profiles based on corporate workers' organizational culture types. To achieve the study's purpose, descriptive statistics, correlation analysis, and latent profile analysis (LPA) were conducted using 253 Korean corporate workers' data. As a result of the analysis, the latent profiles of organizational culture recognized by corporate workers were classified into 5 profiles. We named each profile as high compete-low create culture, high compete-low collaborate culture, collaborate culture, high create culture, and high control culture based on each profile's organizational culture perception. The ANOVA test found statistically significant differences between the groups in the gender equality consciousness. This study is meaningful in that it reveals the relationship between the organization culture perception and the gender equality consciousness and suggested implications for the organizational-level intervention to improve the gender equality consciousness. It will provide basic data for your research.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.2
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• pp.123-129
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• 2000

A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis menium ion. The semi-fed-batch operation was employer to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.3
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• pp.1153-1162
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• 2021

The innovation of enterprises allowed firms to promote technological innovation as an important choice to improve sustainable competitiveness. This study aims to investigate the relationship between absorptive capacity and innovation performance of Chinese high-tech enterprises and focuses on the mediating role of innovation culture in high-tech enterprises. Data came from surveying high-tech enterprises in China, and the reliability analysis, factor analysis, and correlation analysis, path analysis (SEM) were analyzed using SPSS23, AMOS. The results show that intellectual capital composed of human capital, structural capital, and relational has a significant impact on acquisition performance; intellectual capital is composed of human capital; structural capital has a significant influence on innovation performance; and absorptive capital has a significant impact on innovation performance. In addition, innovative culture plays a partial mediating role between absorptive capacity and innovation performance. The findings of this study suggest that, to ensure the better absorption and operation of knowledge, high-tech enterprises can accumulate more knowledge, promote the transformation of knowledge into technology, and strengthen the capability of knowledge absorptive capacity, and at the same time, create an innovation culture atmosphere and encourage employees to develop new products to achieve enterprise goals in order to promote the improvement of innovation performance.

• The Research Journal of the Costume Culture
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• v.10 no.1
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• pp.13-27
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• 2002

Applying H. Gans' taste culture theory to the comtemporary fashion phenomena this study had an object to classify the fashion phenomena in Korea and Japan from 1995 to 1999, and look into and compare them. First of all, the characters of each fashion group of the two countries show that the details of the Korean traditional costume are grafted into the most popular style in each season in Korean high fashion and oriental look used the formative method far Japanese traditional costume and Tokyo street style are reflected in the Japanese high fashion. Mass fashion in Korea equally comes under the influence of European high fashion, Korean street fashion, and Japanese mass fashion, And mass fashion in Japan reflects European high fashion and japanese street fashion. The Street fashion in Korea was affected by Korean entertainers'fashion, 'Tongdaemun market fashion' in Seoul, and Japanese street fashion. And street fashion in Japan is also affected by the pursuit of powerful personality, the absolute imitation if Japanese entertainers' fashion, and 'Tongdaemun market fashion'. All of two countries exercise considerable influence over mass fashion each other.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.560-567
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• 2008

An efficient method to produce water-soluble polysaccharides from Lentinus lepideus is described. The productivity of both endopolysaccharides (PPS) and exopolysaccharides (EPS) was compared under various culture conditions. The effect of treating their own PPS and EPS on immune cytokine production was also studied in relation to culture factors. High yield production of EPS required a moderate culture temperature $(25^{\circ}C)$ as well as long culture period (16-20 days). In contrast, PPS production required a high culture temperature $(30^{\circ}C)$ and short culture period (8 days). Most of the carbon sources did not affect polysaccharides and mycelial production except for sucrose. Immune cytokine levels in the EPS treatment varied among carbon sources or culture periods. PPS did not appear to affect much on the production of cytokines, regardless of the culturing factors, except for the culture period. These results suggest that the optimal culture conditions for L. lepideus vary according to culture purposes, and different culture conditions should be used for different targets including mycelial biomass, EPS, and PPS. Whereas the immunomodulating activitiy of EPS appeared to be affected by culture conditions in L. lepideus, that of PPS did not.

• Korean System Dynamics Review
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• v.16 no.3
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• pp.5-29
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• 2015

The importance of the concept of safety culture has increased in the security of high-risk facility after Chernobyl accident in 1986. This paper elaborated the concept of safety culture and its main factors by Causal Loop Diagram. Due to the decline of safety culture, the occurrence of incidents and accidents require more and more corrective actions to the members of high-risk facilities and thereby increasing their workloads. Employees who must complete the task within the given time have to have time pressures and don't comply with the rules and procedures. Also, a schedule pressure is a big stress for employees, causing mistakes in precision work. In order to improve these problems, CLD of the safety culture in this paper suggests hiring more workers, re-allocation of given workloads and strengthen the learning, communication capabilities and safety leadership. In addition, the two real accident cases were analyzed to test the feasibility of the System Dynamic simulation model through the process of structuring the fault trees on the stationary black out accident in Kori unit 1 in South Korea and Kleen Energy power station explosion in US. The simulation results show that the various safety factors cause the serious accident combined with mechanical failure and safety culture will reduce the possibility of the accidents in these high-risk organizations. This simulation model can contribute to analyzing the impact of the organizational and human factors of safety culture and can provide the alternatives in high-risk facilities.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.6
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• pp.753-757
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• 1999

The growth of rotifer, Brachionus rotundiformis was evaluated at different culture conditions. Rotifer was fed on condensed freshwater Chlorella. The productivity of rotifer in the high density culture system was compared to that of rotifer in the batch culture system, in which rotifer was fed on baker's yeast. The growth rate of rotifer increased as temperature increased in the culture system supplied with air or oxygen gas. The maximum density of rotifer in the culture systems supplied with air was in range of 16,300$\~$17,000 ind./ml at $24^{\circ}C$. In the culture systems supplied with oxygen gas, it ranged 26,300$\~$30,500 ind/ml at $28^{\circ}C$. When the concentration of dissolved oxygen in the culture system supplied with air reached to below 1 ppm or when the concentration of undissolved ammonia in the culture system supplied with oxygen gas reached 16.6$\~$22.6 ppm, the growth of rotifer decreased. When oxygen gas was supplied and pH was adjusted to 7, the maximum density of rotifer reached to 43,000 ind/ml at $32^{\circ}C$. The production costs for 10 billion rotifer in the high density culture and batch culture were 693,000 and 961,000 won, respectively. Therefore, it was concluded that the productivity of rotifer in the high density culture was higher than that in a batch culture.

• The Journal of Information Systems
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• v.24 no.1
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• pp.45-66
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• 2015

This study empirically investigated the relationships among organizational culture, target costing, knowledge management activities, and corporate innovations. The results of this study showed that innovative and supportive culture positively affects the adoption degrees of target costing. According to the results, it was observed that target costing as well as innovative and supportive culture have a positive impact on levels of knowledge management activities(i.e., knowledge creation, sharing, storage, and application). It was also demonstrated that organizational culture has an indirect effect on activation of knowledge management activities through target costing. Thus, to enhance knowledge management activities, target costing must be aligned with appropriate types of organizational culture. In examining the impact of knowledge management activities on the frequencies of product and process innovations, no significant effect was found. Additional analyses that compare across three groups(i.e., low level group, middle level group and high level group in knowledge management activities) and between two groups(i.e., between high level group and middle level group or between high level group and low level group) were performed. The results of comparison showed that the degrees of product and process innovations are highest in high level group, but no significant differences are found in the degrees of innovations between middle level group and low level group.

• Journal of Veterinary Science
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• v.21 no.1
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• pp.13.1-13.14
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• 2020

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.