Biotechnology and Bioprocess Engineering:BBE

  • 제10권3호
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  • Pages.198-204
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  • 2005
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Variability of Azadirachtin in Azadirachta indica (neem) and Batch Kinetics Studies of Cell Suspension Culture

  • Prakash Gunjan (Plant Cell Culture Laboratory, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi) ;
  • Emmannuel C.J.S.K. (Arid Forest Research Institute) ;
  • Srivastava Ashok K. (Plant Cell Culture Laboratory, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi)
  • 발행 : 2005.06.01
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초록

Seeds of neem were collected from different parts of India and analyzed for their azadirachtin content by High Performance Liquid Chromatography (HPLC). In order to assess the effects of genotypic and geographical variation on azadirachtin content in cell cultures, callus development was attempted in the seeds containing high and low concentration of azadirachtin. The concentration of azadirachtin in callus cultures was significantly affected by the explant source. Seed kernels with higher azadirachtin content produced higher azadirachtin content in callus cultures and lower azadirachtin content was seen in callus cultures produced from seed kernels with low azadirachtin content. The protocol for development of elite stock culture of Azadirachta indica was established with the objective of selecting a high azadirachtin-producing cell line. The highest azadirachtin-producing cell line was selected and the effects of different media and illumination conditions on growth and azadirachtin production were studied in shake flask suspension culture. Detailed batch growth kinetics was also established. These studies provided elite starter culture and associated protocols for cultivation of A. indica plant cell culture in the bioreactor.

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참고문헌

  1. Govindchari, T. R. (1992) Chemistry and biological investigation on Azadirachta indica (the neem tree). Curr. Sci. 63: 117-121
  2. Koul, O. M., B. Isman, and C. M. Ketkar (1990) Properties and uses of neem, Azadirachta indica. Can. J. Bot. 68: 1-11 https://doi.org/10.1139/b90-001
  3. Jacbson, M. (1988) Focus on phytochemical pesticides. pp. 101-140. In: M. Jacbson (ed.). The neem Tree. CRC Press, Boca Raton Fla, USA
  4. Schmutterer, H. (1990) Properties and potential of natural pesticides from the neem tree, Azadirachta indica. Ann. Rev. Entomol. 35: 271-297 https://doi.org/10.1146/annurev.en.35.010190.001415
  5. Mordue, A. J. and A. Blackwell (1993) Azadirachtin: An update. J. Insect Physiol. 39: 903-924 https://doi.org/10.1016/0022-1910(93)90001-8
  6. Benge, M. D. (1989) Cultivation and propagation of the neem tree. pp. 1-18. In: M. Jacbson (ed.). Focus on Phytochemical Pesticides, The neem Tree. CRC Press, Boca Raton, USA
  7. Ermel, K. (1995) Azadirachtin content of neem seed kernels from different regions of the world. pp. 222-230. In. M. Schmutterer (ed.). The neem Tree: Source of Unique Natural Products for Integrated Pest Management, Medicine, Industry and Other Purposes. VCH, Weinheim, Germany
  8. Kumar, J. and B. S. Parmar (1997) Neem oil content and its key chemical constituents in relation to the agroclimatic factors and regions of India. Pestic. Res. J. 9: 216-225
  9. Prakash, G., S. S. Bhojwani, and A. K. Srivastava (2002) Production of azadirachtin from plant tissue culture: State of the art and future prospects. Biotechnol. Bioprocess Eng. 7: 185-193 https://doi.org/10.1007/BF02932968
  10. Alfermann, A. W. and M. Petersen (1995) Natural product formation from plant cell biotechnology. Plant Cell Tissue Org. Cult. 43: 199-205 https://doi.org/10.1007/BF00052176
  11. Fowler, M. W. and A. H. Scragg (1998) Natural Products from higher plants and plant cell culture. pp. 165-177. In: M. S. S. Pais, F. Marituna, and J. M. Novais (eds.). Plant Cell Biotechnology NATO ASI Series. Berlin: Springer Verlag, Germany
  12. Constable, F., W. G. W. Kurz, and J. P. Kutney (1982) Variation in cell cultures of perwinkle, Catharanthus roseus. pp. 301-314. In: A. Fujiwara and M. Maruzen (eds.). Plant Tissue Culture. Tokyo, Japan
  13. Evans, D. A., W. R. Sharp, and H. P. Medina-Filho (1984) Somaclonal and gametoclonal variation. Am. J. Bot. 71: 759-774 https://doi.org/10.2307/2443467
  14. Sidhu, O. P. and H. M. Behl (1996) Seasonal variation in azadirachtin in seeds of Azadirachta indica. Curr. Sci. 70: 1084-1085
  15. Sidhu, O. P., V. Kumar, and H. M. Behl (2003) Variability in neem (Azadirachta indica) with respect to azadirachtin content. J. Agric. Food Chem. 51: 910-915 https://doi.org/10.1021/jf025994m
  16. Chattopadhyay, S., S. Farkya, A. K. Srivastava, S. S. Bhojwani, and V. S. Bisaria (2002) Bioprocess Considerations for production of secondary metabolites by plant cell suspension cultures. Biotechnol. Bioprocess Eng. 7: 138-149 https://doi.org/10.1007/BF02932911
  17. Zenk M. K., H. Shagi (El), H. Arens, J. Stockgt, E. J. W. Weiter, and B. Dens (1977) Formation of indole alkaloids serpantine and ajmalicine in cell cultures of Catharanthus roseus. pp. 27-44. In: W. Barz, E. Reinhard, and M. K. Zenk (eds.). Plant Tissue Culture and Its Biotechnological Application. Berlin: Springer-Verlag, Germany
  18. Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  19. Bhojwani S. S. and M. K. Rajdan (1996) Production of secondary metabolites. pp. 537-562. In: Plant Tissue Culture. Theory and Practice. Elsevier, NY, USA
  20. Miller, G. M. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428 https://doi.org/10.1021/ac60147a030
  21. Kaushik, N. (2002) Prospecting chemical diversity of neem (Azadirachta indica) trees growing in India for value addition. Proceedings of 3rd World neem Conference. India
  22. Wewetzer, A. (1998) Callus culture of Azadirachta indica and their potential for the production of azadirachtin. Phytoparasitica 26: 47-52 https://doi.org/10.1007/BF02981265
  23. Smollny, T., H. Wichers, S. Kalenberg, A. Shahsavar, M. Petersen, and A. W. Alfermann (1998) Accumulation of podophyllotoxin and related lignans in cell suspension culture of Linum album. Phytochem. 48: 975-979 https://doi.org/10.1016/S0031-9422(97)00957-6
  24. Fujita, Y., S. Takahasi, and Y. Yamada (1985) Selection of cell lines with high productivity of shikonin derivatives by protoplast culture of Lithospermum erythorhizon cells. Agric. Biol. Chem. 49: 1755-1759 https://doi.org/10.1271/bbb1961.49.1755
  25. Aiken, M. M. and M. M. Yeoman (1986) A rapid screening technique for the selection of high yielding capsaicin cell lines of Capasicum frutescence Mill. In: P. Morris (ed.). Secondary Metabolism in Plant Cell Cultures. Cambridge University Press, London, UK
  26. Hara, Y., T. Laugel, T. Morimoto, T. J. Hiratsuka, T. Yoshioka, Y. Fujita, and Y. Yamada (1989) Flow cytometric analysis of cellular berberin contents in high-and low producing cell lines of Coptis japonica obtained by repeated selection. Planta Med. 55: 151-154 https://doi.org/10.1055/s-2006-961910
  27. Adamse, P. (1990) Selection of high yielding cell lines Tagets using flow cytometry. pp. 726-731. In: H. J. Nijkamp (ed.). Progress in Plant Cellular and Molecular Biology. Kluwer, Dordecht
  28. Songstad, D. D., K. L. Giles, J. Park, D. Novakovski, E. Epp, and L. Friesen (1990) Use of nurse cultures to select for Papaver somniferum cell lines capable of enhanced sanguinarine production. J. Plant Physiol. 136: 236-239 https://doi.org/10.1016/S0176-1617(11)81672-X
  29. Sundaram, K. M. S. and J. Curry (1996) Effect of UV light absorbers on the photostablization of azadirachtin, a neem based biopesticide. Chemosphere 32: 649-659 https://doi.org/10.1016/0045-6535(95)00370-3
  30. Mathur, A., Y. N. Shukla, M. Pal, P. S. Ahuja, and Uniyal G. C (1994) Saponin production in callus and cell suspension cultures of Panax quinquefolium. Phytochem. 35: 1221-1225 https://doi.org/10.1016/S0031-9422(00)94824-6
  31. Raval, K., S. Hellwig, G. Prakash, A. R. Plasencia, A. K. Srivastava, and J. Buchs (2003) Necessity of a two-stage process for the production of azadirachtin-related limonoids in suspension culture of Azadirachta indica. J. Biosci. Bioeng. 96: 16-22 https://doi.org/10.1016/S1389-1723(03)90091-0
  32. Allan, E. J., J. P. Easwara, and A. Wood (1994) The production of azadirachtin by in vitro tissue culture of neem, Azadirachta indica. Pesti. Sci. 42: 147-152 https://doi.org/10.1002/ps.2780420302
  33. Veersham, C., M. R. Kumar, D. Sowjanya, C. K. Kokate, and S. S. Apte (1998) Production of azadirachtin from callus cultures of Azadirachta indica. Fitoterapia 69: 423-424
  34. Balaji, K. (2001) Studies on Tissue Culture of Azadrachta indica (neem). Ph.D. Thesis. Kakatiya University, Warangal, AP, India
  35. Kearney, M. L., E. J. Allan, J. E. Hooker, and A. J. Mordue (1994) Antifeedant effects of in vitro culture extract of neem tree, Azadirachta indica against the deser locust (Shistocerca gregaria). Plant Cell Tiss. Org. Cult. 37: 67-71 https://doi.org/10.1007/BF00048119
  36. Kuruvilla, T., P. Komaraiah, and S. V. Ramakrishna (1999) Enhanced secretion of azadirachtin by permeabilized margosa (Azadirachta indica) cells. Ind. J. Exp. Bot. 37: 89-91
  37. Balaji, K., C. Veeresham, K. Srisilam, and C. Kokate (2003) Azadirachtin, a novel biopesticide from cell cultures of Azadirachta indica. J. Plant Biotechnol. 5: 121-129
  38. Allan, E. J., J. P. Eeswara, A. Jarvis, A. J. Mordue (luntz), E. Morgan, and T. Stucbbury (2002) Induction of hairy root culture of Azadirachta indica A. Juss and their production of azadirachtin and other important insect bioactive metabolites. Plant Cell Rep. 21: 374-379 https://doi.org/10.1007/s00299-002-0523-3