• 제목/요약/키워드: high culture

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Increased Lipolytic Activity by High-pungency Red Pepper Extract(var. Chungyang) in Rat Adipocytes in vitro

  • Do, Myoung-Sool;Hong, Sung-Eui;Ha, Jung-Heun;Park, Sun-Mi;Ahn, In-Sook;Yoon, Ji-Young;Park, Kun-Young
    • Preventive Nutrition and Food Science
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    • 제9권1호
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    • pp.34-38
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    • 2004
  • High-pungency red pepper and capsaicin modulate circulating hormone levels and induce lipolysis in adipose tissue in vivo. This study was designed to investigate the lipolytic activity of adipocytes by high-pungency red pepper extract in vitro. High-pungency red pepper (var. Chungyang) powder showed 126.1 mg% of capsaicinoid which was 3 x higher than low-pungency red pepper powder (var. Daemyung). To study the effects of high-pungency red pepper extract on lipolytic activity, preadipocytes were separated from the epidermal fat of 14 day-old rats, induced to differentiate into adipocytes and were treated with red pepper extracts. The amount of glycerol released from adipocytes into the culture medium was analysed to measure lipolytic activity. Glycerol release from adipocytes was increased in a dose-dependent manner with high-pungency red pepper extract treatment. However, there was no significant change in the glycerol release when adipocytes were treated with low-pungency red pepper extract. To investigate whether lipolysis by high-pungency red pepper extract is caused by capsaicin, glycerol release was detected after the treatment of adipocytes with capsaicin. Glycerol release was significantly increased by capsaicin. These results suggest that high-pungency red pepper extract might have a direct lipolytic activity in adipocytes that is mediated by capsaicin.

양파 육질 및 껍질의 섭취가 고철분식이를 공급한 노령흰쥐의 항산화능에 미치는 영향 (Effect of Onion Flesh or Peel Feeding on Antioxidative Capacity in 16-Month-old Rats Fed High Iron Diet)

  • 박주연;김미경
    • 한국식생활문화학회지
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    • 제20권6호
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    • pp.721-730
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    • 2005
  • Recently, epidemiological evidence has raised concerns that moderate elevation in body iron stores may increase oxidative stress and the risk of cardiovascular disease and cancer. Onion flesh or peel contains antioxidant such as flavonoids and alk(en)ylcysteine sulphoxides. This study was conducted to examine the effect of onion flesh or peel feeding on antioxidative capacity in aged rats supplemented with high dietary iron. Thirty-two Sprague-Dawley male 16-month-old rats weighing $618{\pm}6g$ were acclimated for 10 days with medium-iron diet (35ppm in diet), and blocked into 4 groups according to their body weights and raised for 3 months on either control diets (adequate iron-35ppm or high iron-350ppm) or experimental diets containing onion flesh/peel (5% w/w in diet) with high iron (350ppm). Rats fed high iron-onion peel diet had significantly high quercetin and isorhamnetin levels in plasma whereas rats fed high iron-onion flesh diet did not show. Plasma TBARS level was lowered by onion flesh or peel diet with high iron supplementation. However, there was no significant difference in cellular DNA damage in brain and kidney tissue among all experimental groups. We concluded that high iron diet (10 times higher than requirement) tend to increase oxidative stress and it is plausible that onion flesh or peel feeding enhances antioxidative capacity in the elderly even with iron supplementation.

서울지역 고등학생들의 외식서비스 특성에 대한 중요인지도와 수행만족도 비교 (Importance-performance Analysis of High School Students in Seoul towards Restaurant Service Attributes)

  • 양일선;이진미;차진아;한재정
    • 한국식생활문화학회지
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    • 제11권5호
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    • pp.663-671
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    • 1996
  • The purpose of this study was to compare high-school students importance and performance toward restaurant service attributes for the marketing strategy development. Specific objectives were to: a) investigate restaurant patronage characteristics of high school students; b) identify the difference of patronage behavior among three types of restaurants; and c) analyze the importance and performance among three types of restaurants. A questionnaire was developed and hand-delivered to 400 students enrolled 9th grade in 4 different high schools in Seoul. A total of 320 students (80%) was responded to this study. The questionnaire was composed of two parts with 47 restaurant service attribute statements. Results of this study were as follows: 1. A total of 57% was female and 61% of respondents spent less than ₩5,000 per week on eating out. 2. The frequency of visiting the low-priced restaurant was 8.9 times per week. 3. Reasons for being a patronage to low- (${\geq}$₩2,000) and mid-priced ( <₩2,000 and ${\geq}$₩5,000) restaurants were hunger, appointment, and seeking favorites with freinds but the reason for high-priced (<₩5,000) restaurants was celebrating special days with parents. 4. The main source of information for selecting restaurants was family and friends, T.V. advertising, and bulletin board. 5. For the low-priced restaurants, food, hygiene, price, and location were rated as important; location, price, menu, and food were rated as satisfied. 6. For the mid-priced restaurants, hygiene, food, price, and menu were rated as important; food, hygiene, service, and menu were rated as satisfied. 7. For the high-priced restaurants, hygiene, food, and atmosphere were rated as important; food, hygiene, atmosphere, and menu were as satisfied. 8. According to paired t-test, the score gap between importance and performance was the highest in the hygiene attribute; differences were high with the low-priced and low with high-priced restaurants.

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명아주(Chenopodium album)와 바랭이( Digitaria sanguinalis)의 경쟁이 에너지 분해에 미치는 효과 (The Effect of Energy Allocation on Competition of Chenopodium album and Digitaria sanguinalis)

  • Park, Bong-Kyu;Kim, Ok-Kyung
    • The Korean Journal of Ecology
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    • 제9권1호
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    • pp.73-78
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    • 1986
  • The effects of density and soil nutrient regime on competition between individuals in pure and mixed populations of two annuals. Chenopodium album and Digitaria sanguinalis were investigated at the level of enegy allocation. Seedling emergence of two species was extremely high (>90%) in both pure and mixed culture irrespective of the density and nutrient regime, but percentage of seedling establishment or fertile plant became gradually low with increasing density owing to 'self-thinning'. The mean plant dry weight was significantly reduced with increasing density and decreasing soil fertility. The dry matter production of D. sanguinalis in mixture was markedly greater than in pure culture under medium and high density. Also, as considered the number of seed production as reproductive allocation, relationship between them and the number of seed production per plant showed a similar tendency. Therefore, these results indicated that D. sanguinalis had a greater competitive advantage than C. album and energy allocations to variious organs were regulated by plastic response rather than determined genetically.

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Fed-batch Production of High-Content RNA Yeast by Using Industrial Medium

  • 김재범;남수완
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 춘계학술발표대회
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    • pp.295-298
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    • 2000
  • In order to maximize the RNA accumulation and biomass production in Saccharomyces cerevisiae MTY62, a high-content RNA yeast strain, fed-bach cultures were performed with optimized industrial medium including molasses and corn steep liquor. Among the feeding modes examined, the constant feeding mode resulted in the cell concentration of 35.7 g-DCW/L and the RNA concentration of 5434 ${\mu}g-RNA/mL$, which were about 2-fold increased levels, compared to the results of bach culture. However, the RNA content (153 mg-RNA/g-DCW) in the fed-batch cultures was lower than that in the batch culture (171 mg-RNA/g-DCW).

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Combined analysis of transcriptome and proteome for high cell density cultivation of Escherichia coli

  • 윤성호;한미정;임근배;이상엽
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2001년도 추계학술발표대회
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    • pp.845-848
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    • 2001
  • For understanding physiology and metabolism under various culture conditions, combined analysis of transcriptome and proteome is attractable way. We have manufactured DNA microarray containing 2,850 genes including all functionally known and putative ones. In this study, we report analysis of transcriptome and proteome during the high cell density culture of E. coli by using DNA microarray and 2-DE. Fed-batch fermentation of E. coli was carried out by exponential feeding of nutrients until the maximum cell density reached 74 g dry cell weight/L (g DCW/L). Changes in transcriptome and proteome during the HCDC are analyzed qualitatively and quantitatively to provide their physiological and metabolic meanings.

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네크라인과 헤어스타일이 얼굴 이미지 및 형태 지각에 미치는 영향 (Effect of Neckline-Hairstyle Combinations on the Perception of Face Image and Type)

  • 이영미;서미아
    • 복식문화연구
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    • 제6권4호
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    • pp.13-25
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    • 1998
  • This study focuses on the visual effects of various neckline-haristyle combinations on the perception of face image and type. The study employed a model with oval face and examined 35 combinations made up of five necklines and seven hairstyles. Looking at various face images depending upon different necklines, in case of round, V, boat square, and high necklines, long wave hair and medium wave hair produced a feminine image of marked individuality; long straight hair that covers the forehead and medium straight hair gave an image of charm and purity as well as an image of neatness; and long straight hair short cut hair showed an intellectual image. Regarding the perceptual type of face depending upon the different necklines of round, V, square, and high, the long straight hair covering the forehead and medium wave hair had the effect of an optical illusion that made the face look short and round; and short cut hair made the face line look distinct and the face look oval and slender.

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비소를 제거한 두부응고제를 사용한 콩의 종류에 따른 두부의 제조 특성 (Characteristics of Soybean Curd according to Various Soybean by Using Soybean Coagulant Removed with Arsenic)

  • 이은숙;최원식
    • 한국산업융합학회 논문집
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    • 제20권5호
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    • pp.441-446
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    • 2017
  • Among the many foods, it is hard to find perfect food with nutrition and functionality like beans. Korean food culture is the main ingredient of korean culture, kochujang, soybean paste, and soy sauce, and processed soybean tofu is the main ingredient. Soybean meets high quality protein and fat, and it has excellent results in prevention and treatment of all kinds of diseases. Soybean food is becoming a new generation health food. In countries where animal protein intake is low, soybean is used as a protein source instead of animal protein. Tofu, a processed food, is a complete food with high digestibility. In order to publicize the superiority of soybean nutritional value, Tofu processing and powder were investigated by observing the size, shape and characteristics of bean powder using domestic soybeans and imported soybean, and the variation of the amount of coagulant.

A Study on the Relationship between Snack Sugar Intake and Dietary Diversity in Elementary School Students

  • Yun, Hye-Jin;Chang, Moon-Jeong
    • Food Quality and Culture
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    • 제2권2호
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    • pp.80-84
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    • 2008
  • In this study, sixth grade school children in the Guri area were surveyed via questionnaire regarding their sociodemographic characteristics, snacking patterns, and intakes of different food groups and sugared snacks. Students with employed mothers had more frequent snacking events and were more dependent on store-purchased snacks compared to those having an unemployed mother. A high consumption of snack sugar was associated with a higher dietary diversity score. There was a positive relationship between snack sugar intake and the intakes of meat and dairy products and foods in the oil and nut group, respectively. A high snack sugar intake had a negative effect on fruit intake. Specific nutrient intake data will be required before conclusions can be made on whether nutrient imbalances are a concern in elementary school children having sweet snacks. Yet, the current data indicate that an increased snack sugar intake can decrease fruit intake, which could result in certain nutrient deficits. Specific dietary guidance may be necessary to address the snacking habits of elementary school children.

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Dextranase 생산균주의 분리, 동정 및 효소생산 (Isolation and Identification of Dextranase Production Strains and Enzyme Production)

  • 이종태;이동희;곽이성;김영호;성현순;김찬조
    • 한국미생물·생명공학회지
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    • 제23권4호
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    • pp.405-410
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    • 1995
  • In order to screen dextranase with high dextranolytic activity from microbial origins, dextranase producing fungal isolates were isolated from soil of the Taeion area. 197 strains with dextranolytic activities were isolated, out of which 3 strains with high dextranolytic activities were selected in the first screening. A strain (GR-98) with a best dextranolytic activity was selected in the second screening. The strain was identified to be similiar Aspergillus ustus by the morphological and cultural characteristics. The optimum culture temperature and initial pH for the dextranase production of the strain was 30$\circ$C and 7.0, respectively. The optimum culture medium was composed of 2% dextran, 0.3% KNO$_{3}$, 0.05% K$_{2}$HPO$_{4}$, 0.02% MgSO$_{4}$-7H$_{2}$O, 0.05% KC1, and 2.5 $\mu$g/ml pyridoxamine, and the enzyme production was maximum when the strain was subcultured at 30$\circ$C for 7 days.

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