• 한국작물학회지
• /
• 제7권1호
• /
• pp.85-92
• /
• 1969

우리나라 수도작개량의 방향은 단위당 수량을 향상시키되 어디까지나 지역전체에 고도기술의 적용으로 고단수를 평준화시키면서 능률면에서도 공동작업관리로 이끌어 노동생산성을 높혀야 한다는데 있으므로 이를 구현하는 생산체제인 수도집단재배의 수행을 위한 이론을 연구하고 그 발전을 위한 기대효과와 전망을 구명하여야 하는바 현재까지의 결과를 요약하면 다음과 같다. 1. 수도작지대에서 고단수의 평준화를 위한 생산기술을 보급하고 고능율의 경영을 위해서는 집단재배가 가장 효과적인 방법의 하나이다. 2. 효과적인 집단재배를 실천하기 위하여는 단지의 수리, 토양, 품종 기타 영농상황을 보다 체계화하여야 한다. 3. 이와같은 기간재배기술은 경작농가의 협의에 의하여 재배력을 작성하고 단지내에서의 모든 재배법은 재배력에 의하여 통일되며 기간기술의 작업은 공동으로 하고 기타 세부작업은 개발로 한다. 4. 개별경영에서는 충실하게 도입되기 어려웠던 기술이 집단재배에서는 용이하게 도입될 수 있었으며 1968년도의 전국 집단재배 성적을 보면 일반재배에 비하여 평균 23.3%의 증수를 보이고 있다. 5. 현재 우리나라 집단재배의 형태는 초보적인 협정 영농형을 중심으로 추진되고 있는데 일부작업에는 공동작업형 및 청부작업형도 드물게 결합되고 있는 것이 보이고 있으나 앞으로 보다 안정다수확을 위한 성력화 또는 대규모의 기계화를 위해서는 공동작업형을 촉진시키면서 입지조건에 따라 기술신탁형이나 청부작업형을 결합시켜 나가는 방향으로 발전되어야 할것이다.

• Reproductive and Developmental Biology
• /
• 제31권2호
• /
• pp.91-96
• /
• 2007

In vitro development of porcine embryo is affected by culture condition. One possible factor is osmolarity of culture medium. 1his study examined whether high osmolarity of culture medium at the early culture stage improves development of preimplantation porcine in vitro fertilization (IVF) and nuclear transfer (NT) embryos. NT and IVF embryos were divided into three groups and the basic medium was PZM-3 ($250{\sim}270$ mOsmol, control group). The control group of embryos was cultured in PZM-3 for whole culture period. Other two groups of embryos were cultured in a modified PZM-3 with 0.05 M sorbitol or 0.05 M sucrose ($300{\sim}320$ mOsmol, sorbitol or sucrose group) for the first 2 days, and then cultured in PZM-3 for further culture. NT embryos cultured in sucrose group showed a significantly higher developmental rate to the blastocyst stage with a decreased apoptosis rate compared to the sorbitol (p<0.05). For IVF, sucrose group showed a significantly increased the blastocyst formation rate with a decreased apoptosis rate compared to the control (p<0.05). This study represents that the high osmolarity in the early embryo culture stage can enhance the in vitro development of porcine NT and IVF embryos to the blastocyst stage with reduced apoptosis of cells.

• 생물환경조절학회지
• /
• 제7권2호
• /
• pp.144-150
• /
• 1998

PUR 배지와 펄라이트 배지를 이용하여 방울토마토를 재배할 경우 적산일사량에 따른 관수가 과실의 당도에 미치는 효과에 대해서 연구하였다. PUR 배지경의 경우 관수량이 많을수록 당도가 낮은 경향을 보였으며 상위화방으로 갈수록 차이가 크게 나타났다 배액량을 25%로 했을 때 펄라이트 배지에 비해 PUR 배지에서 당도가 높게 나타났으며 생육이 진행됨에 따라서 그 차이가 커졌다. 과실의 붉은 정도를 나타내는 a＊값은 PUR 배지간에는 관수량에 따라 큰 차이를 보이지 않았으나 펄라이트 배지와는 상당한 차이를 보여 3, 4, 5화방에서는 뚜렷하게 PUR이 높은 값을 나타내었다. 과중은 펄라이트 재배에서 높았으며 PUR 배지경에서는 배액량을 25%로 하는 것이 고당도 과실을 다수확 할 수 있는 것으로 나타났다.

• 한국동물생명공학회지
• /
• 제34권2호
• /
• pp.130-138
• /
• 2019

Although somatic cell nuclear transfer (SCNT)-derived embryonic stem cells (ESCs) in pigs have great potential, their use is limited because the establishment efficiency of ESCs is extremely low. Accordingly, we tried to develop in-vitro culture system stimulating production of SCNT blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts for enhancing production efficiency of porcine ESCs. For these, SCNT blastocysts produced in various types of embryo culture medium were cultured in different ESC culture medium and optimal culture medium was determined by comparing colony formation efficiency. As the results, ICM of porcine SCNT blastocysts produced through sequential culture of porcine SCNT embryos in the modified porcine zygote medium (PZM)-5 and the PZM-5F showed the best formation efficiency of colonies in α-MEM-based medium. In conclusion, appropriate combination of the embryo culture medium and ESC culture medium will greatly contribute to successful establishment of ESCs derived from SCNT embryos.

• 농촌계획
• /
• 제22권1호
• /
• pp.25-35
• /
• 2016

This study targets Korean culture theme park in Yeongju-Zone and World confucian scholar culture park in Bonghwa-Zone among the Three Major Cultural Areas Projects supervised by Ministry of Culture, Sports and Tourism. The study analyzes the Historical and Cultural Authenticity of each project on the basis of the reports and the report materials for meeting with related organizations. The results of the study are drawn as follows. First, through the theoretical consideration, this study drew the types of authenticity: originality, identity, specificity and visibility, which are evaluation items on the Historical and Cultural Authenticity. Second, Bonghwa-Zone succeeded in acquiring originality with tangible cultural properties but Yeongju-Zone chose a project site without it. Third, with originality, Bonghwa-zone was evaluated as having resources and concept with high traditional culture connectivity and fulfilled identity. It led the feature showing the high affinity between originality and identity. Fourth, compared to the projects of Andong-Zone in the Three Major Cultural Areas Projects, these two projects failed to acquire the distinctions since the primary and the secondary influence area and major facilities & programs coincided with those of the projects of Andong-Zone. Fifth, compared to Bonghwa-Zone, Yeongju-Zone realized visibility faithfully by the conceptual flexibility of "Korean Culture" and a large-scale development. Sixth, in terms of the Historical and Cultural Authenticity of project plan, it is evaluated that Yeongju-Zone and Bonghwa-Zone only fulfilled visibility and specificity respectively.

• 복식문화연구
• /
• 제6권2호
• /
• pp.77-91
• /
• 1998

The present study collected and analyzed the Korean's folk-belief related to Koran traditional costume culture which are handed down from ancestor and are known to present Korean society as of 1990's. The purpose of the present study is to understand emotion and the view of Korean people, and to understand the social roles of the costumes in Koran traditional society more deeply and more comprehensively. The study was conducted by document analysis and interview. The results of the study are summerized as follows: Folk-belief related to spinning(gilssam), sericultural industry and weaving are mostly enduced from the hope for god quality of work and acquisition of good skill of weaving. There has been also a belief hat gilssam has a magic power to easy delivery and fecundity. Most Folk-belief of taboo related to clothing constraction proposed to people pay great care while they are sawing. Some ways of management of clothing has been related to the manners of which are associated with the differentiation between high and low level and predominance of men over women that was originated fro m confucianism. Socks(busun) and shoes are also considered to have magic power to prevent from exorcism and demon of ill health. This study suggests that folk-belief give us the wisdom a guideline and lessons for better life, high quality arts and techniques which are handed down from ancestors. Therefore, folk-beliefs having a great value, non-shaped treasures of traditional culture heritage should be collected as many as possible, recorded, and preserved.

• Journal of Microbiology and Biotechnology
• /
• 제10권4호
• /
• pp.470-475
• /
• 2000

Genetic chracterstics of the structural gene of guamerin (a novel elastase inhibitor from Korean leech), integrated into the HIS4 locus of chromosomal DNA of Pichia pastoris along with the $\alpha$-factor leader sequence, were investigated. In the selected clone from candidates, two copies of the integration cassette including the structural gene copies of the integration cassette including the structural gene of guamerin were found in the integration site of the chromosomal DNA of P.pastoris. It was demonstrated that the integrated structural gene of guamerin was stable up to about 70 generations in the relay flask culture. Then, a high-cell-density culture could be fulfilled easily by DO-stat fed-batch culture, in which the cell growth and the recombinant guamerin production reached about 250 of OD600nm and 260 mg/l, respectively. Finally, it was revealed that the DNA sequence of the integrated structural gene of guamerin in P. pastoris was maintained correctly in the end of production cells of relay flask culture and high-cell-density culture.

• Plant Resources
• /
• 제6권1호
• /
• pp.1-6
• /
• 2003

Ginseng(Panax ginseng C. A. Meyer) is important medicinal plant but requires 4-year cultivation for root harvest because of slow growth. In contrast, ginseng hairy roots induced by introducing Ri-plasmid of Agrobacterium rhizogenes into genomic DNA of plant cells show vigorous growth, and the hairy roots produce the same or more saponins than natural ginseng roots. Therefore, hairy roots can be used for commercial purposes. The present study was carried out to induce hairy roots with both active growth and high saponin contents. Numerous hairy roots of Panax ginseng were obtained after root disks of three-year old roots were infected with Agrobacterium rhizogenes R1000 A4T in dark condition after one month of culture. About 3 hundred lines of hairy roots were selected according as morphological characters on medium with carbenicillin. After pre-selection of fifteen lines of hairy roots with active growth, KGHR-l and KGHR-8 lines were finally selected which had characters of high content of ginsenoside-Rd and ginsenoside-Re, respectively. The optimum growth of hairy roots was achieved in the culture of 1/2 MS liquid medium in dark (22 $^{\circ}C$) under 60 rpm gyratory shaking. Hairy roots grew well in 5L Erlenmeyer flasks, lL roller drums, 10L jar-fermenters, and especially in 20L air-lift culture vessels.

• 식물조직배양학회지
• /
• 제25권5호
• /
• pp.319-322
• /
• 1998

여러 계통의 고추(Capsicm annuum L.)의 약을 이용하여 식물체 재분화 조건을 확립하였다. 꽃받침 위로 꽃잎이 2mm 올라온 미성숙 화뢰에서 상부로부터 절반 이하로 착색된 약을 채취하여 0.1mg/L 2,4-D와 0.1mg/L kinetin이 첨가된 Dumas 고체배지에 치상하였다. 배양 4주후부터 배가 발생하였으며, 8주후의 배발생 빈도는 최고 58.3%였다. 배는 MS 기본배지로 옮겨주었을 때 95이상이 발아하여 식물체로 재분화되었다.

• 패션비즈니스
• /
• 제4권4호
• /
• pp.55-70
• /
• 2000

The purpose of this thesis is to suggest the new orientalism fashion through the comprehensive approach to analyze the art of the Tang dynasty, China. Tang dynasty has high culture, economy, polity and art. Also the character of Tang's culture is very international. Therefore that point is coincide with fusion culture in the early 2000's. As a method of accomplishing this research, the documents and fashion magazines related to the art and fashion were examined. Through analyzing the art in the Tang's related to fusion culture, reach a conclusion that the modern fashion has four characters. 1. The cause of high culture and economy, the trend of fashion was decorative 2. The cause of realism, the trend of fashion were modern and simple. 3. The cause of rhythmical line, the trend of fashion had natural drape. 4. The cause of globalism, the trend of fashion mixed east with west This research keep going for showing new orientalism and coinciding with 21th century's trend.