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Effect of Osmolarity of Culture Medium on the Preimplantation Development of Porcine NT and IVF Embryos  

Hwang, In-Sun (National Livestock Research Institute, RDA)
Park, Mi-Rung (National Livestock Research Institute, RDA)
Moon, Hyo-Jin (National Livestock Research Institute, RDA)
Shim, Joo-Hyun (National Livestock Research Institute, RDA)
Kim, Dong-Hoon (National Livestock Research Institute, RDA)
Yang, Byoung-Chul (National Livestock Research Institute, RDA)
Ko, Yeoung-Gyu (National Livestock Research Institute, RDA)
Yang, Boh-Suk (National Livestock Research Institute, RDA)
Cheong, Hee-Tae (School of Veterinary Medicine. Kangwon National University)
Im, GI-Sun (National Livestock Research Institute, RDA)
Publication Information
Reproductive and Developmental Biology / v.31, no.2, 2007 , pp. 91-96 More about this Journal
Abstract
In vitro development of porcine embryo is affected by culture condition. One possible factor is osmolarity of culture medium. 1his study examined whether high osmolarity of culture medium at the early culture stage improves development of preimplantation porcine in vitro fertilization (IVF) and nuclear transfer (NT) embryos. NT and IVF embryos were divided into three groups and the basic medium was PZM-3 ($250{\sim}270$ mOsmol, control group). The control group of embryos was cultured in PZM-3 for whole culture period. Other two groups of embryos were cultured in a modified PZM-3 with 0.05 M sorbitol or 0.05 M sucrose ($300{\sim}320$ mOsmol, sorbitol or sucrose group) for the first 2 days, and then cultured in PZM-3 for further culture. NT embryos cultured in sucrose group showed a significantly higher developmental rate to the blastocyst stage with a decreased apoptosis rate compared to the sorbitol (p<0.05). For IVF, sucrose group showed a significantly increased the blastocyst formation rate with a decreased apoptosis rate compared to the control (p<0.05). This study represents that the high osmolarity in the early embryo culture stage can enhance the in vitro development of porcine NT and IVF embryos to the blastocyst stage with reduced apoptosis of cells.
Keywords
Osmolarity; NT embryos; Sorbitol; Sucrose; Apoptosis;
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1 Byrne AT, Southgate J, Brison DR, Leese HJ (1999): Analysis of apoptosis in the preimplantation bovine embryo using TUNEL. J Reprod Fertil 117:97-105   DOI   ScienceOn
2 Hardy K, Spanos S, Becker D, Iannelli P, Winston RM, Stark J (2001): From cell death to embryo arrest: mathematical models of human preimplantation embryo development. Proc Natl Acad Sci USA 98: 1665-1660
3 Kano K, Miyano T, Kato S (1998): Effects of glycosaminoglycans on the development of in vitromatured and -fertilized porcine oocytes to the blastocyst stage in vitro. Biol Reprod 58:1226-1232   DOI   ScienceOn
4 Mckiernan SH, Bavister BD (1990): Environmental variables influencing in vitro development of hamster 2-cell embryos to the blastocyst stage. Biol Reprod 43:404-413   DOI   ScienceOn
5 Neuber E, Luetjens CM, Chan AWS, Schatten GP (2002): Analysis of DNA fragmentation of in vitro cultured bovine blastocysts using TUNEL. Theriogenology 57:2193-2202   DOI   ScienceOn
6 Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H, Perry ACF (2000): Pig cloning by microinjection of fetal fibroblast nuclei. Science 289:1188-1190   DOI   ScienceOn
7 Wang WH, Abeydeera LR, Prather RS, Day BN (2000): Actin filament distribution in blocked and developing pig embryos. Zygote 8:353-358   DOI   ScienceOn
8 Miyoshi K, Funahashi H, Okuda K, Niwa K (1994): Development of rat one-cell embryos in a chemically defined medium: effects of glucose phosphate and osmolarity. J Reprod Fertil 100:21-26   DOI   ScienceOn
9 Funahashi H, Kim NH, Stumpf TT, Cantley TC, Day BN (1996): Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. Biol Reprod 54:1412-1419   DOI   ScienceOn
10 Liu L, Trimarchi JR, Keef DL (2002): Haploid but parthenogenetic activation leads to increased incidence of apoptosis in mouse embryos. Biol Reprod 66:204-210   DOI   ScienceOn
11 Mercola M, Stiles CD (1998): Growth factor superfamilies and mammalian embryogenesis. Development 102:451-460
12 Nguyen VT, Kure-bayashi S, Harayama H, Nagai T, Miyake M (2003): Stage specific effects of the osmolarity of a culture medium on the development of parthenogenetic diploids in the pig. Theriogenology 59:719-734   DOI   ScienceOn
13 Walker SC, Shin TY, Zaunbrecher GM, Romano JE, Johnson GA, Bazer FW, Piedrahita JA (2002): A highly efficient method for porcine cloning by nuclear transfer using in vitro-matured oocytes. Cloning Stem Cells 4:105-112   DOI   ScienceOn
14 Petters RM, Johnson BH, Reed ML, Archibong AE (1990): Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro. J Reprod Fertil 89:269-275   DOI   ScienceOn
15 Feugang JM, Roover DE, Leonard S, Dessy F, Donnay I (2002): Kinetics of apoptosis in preimplantation bovine embryos produced in vitro and in vivo. Theriogenology 57:494
16 Naglee DL, Maurer RA, Foote HR (1969): Effect of osmolarity on in vitro of rabbit embryos in a chemically defined medium. Exp Cell Res 58:331-333   DOI
17 Gjorret JO, Wengle J, King WA, Schellander K, Hyttel P (2002): Occurrence of apoptosis in bovine embryos reconstructed by nuclear transfer or derived in vivo. Theriogenology 57:495
18 Abeydeera LR, Wang WH, Cantley TC, Rieke A, Murphy CN, Prather RS, Day BN (2000): Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology 54:787-797   DOI   ScienceOn
19 Betthauser J, Forsberg E, Augenstein M, Childs L, Eilertsen K, Enos J, Forsythe T, Golueke P, Jurgella G, Koppang R, Lesmeister T, Mallon K, Mell G, Misica P, Pace M, Pfister-Genskow M, Sterlchenko N, Voelker G, Watt S, Thompson S and Bishop M (2000): Production of cloned pigs from in vitro systems. Nat Biotechnol 18:1055-1059   DOI   ScienceOn
20 Kim MK, Kwon DJ, Park CK, Yang BK (2005): Effects of culture medium and osmolarity on in vitro maturation of follicular oocytes and development of parthenogenetic embryos in porcine. Reprod Dev Biol 29:169-174   과학기술학회마을
21 Levy RR, Cordonier H, Czyba JC, Goerin JF (2001): Apoptosis in preimplantation mammalian embryo and genetics. Int J Anat Embryol 106 (suppl) 2:101-108
22 Abeydeera LR, Wang WH, Cantley TC (1998a): Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Mol Reprod Dev 51:395-401   DOI   ScienceOn
23 Hardy K, Handyside AH, Winston RML (1989): The human blastocyst: cell number, death and allocation during late preimplantation development in vitro. Development 107:597-604
24 Jurisicova A, Latham KE, Casper RF, Varmuza SL (1998): Expression and regulation of genes associated with cell death during murine pre-implantation embryo development. Mol Reprod Dev 51: 243-253   DOI   ScienceOn
25 Andrew JK, Abeydeera LR, Alvarez IM, Day BN, Buhi WC (2000): Effects of the porcine oviductspecific glycoprotein on fertilization, polyspermy, and embryonic development in vitro. Biol Reprod 63: 242-250   DOI   ScienceOn
26 Brinster RL (1965): Study on the development of mouse embryos in vitro I : the effect of osmolarity and hydrogen ion concentration. J Exp Zool 158:49-58   DOI   ScienceOn
27 Im GS, Lai L, Liu Z, Hao Y, Wax D, Bonk A, Prather RS (2004): In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Theriogenology 61:1125-1135   DOI   ScienceOn
28 Whitten WK (1971): Nutrient requirements for the culture of preimplantation embryos in vitro. In: Raspa G (ed) Advances in the Bioscience. Oxford: Pergamon Press 6:129-141
29 Gandhi AP, Lane M, Gardner DK, Krisher RL (2001): Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture media. Mol Reprod Dev 58:269-275   DOI   ScienceOn
30 Bavister BD, Leibfried ML, Lieberman G (1983): Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod 28:235-247   DOI   ScienceOn
31 Im GS, Yang BS, Lai L, Liu Z, Hao Y, Prather RS (2005): Fragmentation and development of preimplantation porcine embryos derived by parthenogenetic activation and nuclear transfer. Mol Reprod Dev 71(2):159-165   DOI   ScienceOn
32 Abeydeera LR, Wang WH, Cantley TC, Ridke A, Day BN (1998b): Co-culture with follicular shell pieces can enhance the developmental competence of pig oocytes after in vitro fertilization: relevance to intracellular glutathione. Biol Reprod 58:213-218   DOI   ScienceOn