Browse > Article
http://dx.doi.org/10.12750/JARB.34.2.130

Establishment of In-Vitro Culture System for Enhancing Production of Somatic Cell Nuclear Transfer (SCNT) Blastocysts with High Performance in the Colony Formation and Formation of Colonies Derived from SCNT Blastocysts in Pigs  

Han, Na Rae (Department of Animal Life Science, Kangwon National University)
Baek, Song (Department of Animal Life Science, Kangwon National University)
Lee, Yongjin (College of Veterinary Medicine, Kangwon National University)
Lee, Joohyeong (Institute of Veterinary Medicine, Kangwon National University)
Yun, Jung Im (Institute of Animal Resources, Kangwon National University)
Lee, Eunsong (College of Veterinary Medicine, Kangwon National University)
Lee, Seung Tae (Department of Animal Life Science, Kangwon National University)
Publication Information
Journal of Animal Reproduction and Biotechnology / v.34, no.2, 2019 , pp. 130-138 More about this Journal
Abstract
Although somatic cell nuclear transfer (SCNT)-derived embryonic stem cells (ESCs) in pigs have great potential, their use is limited because the establishment efficiency of ESCs is extremely low. Accordingly, we tried to develop in-vitro culture system stimulating production of SCNT blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts for enhancing production efficiency of porcine ESCs. For these, SCNT blastocysts produced in various types of embryo culture medium were cultured in different ESC culture medium and optimal culture medium was determined by comparing colony formation efficiency. As the results, ICM of porcine SCNT blastocysts produced through sequential culture of porcine SCNT embryos in the modified porcine zygote medium (PZM)-5 and the PZM-5F showed the best formation efficiency of colonies in α-MEM-based medium. In conclusion, appropriate combination of the embryo culture medium and ESC culture medium will greatly contribute to successful establishment of ESCs derived from SCNT embryos.
Keywords
alpha-minimum essential medium; embryonic stem cells; pig; porcine zygotic medium; somatic cell nuclear transfer;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Rubessa M, Polkoff K, Bionaz M, Monaco E, Milner DJ, Holllister SJ, Goldwasser MS, Wheeler MB. 2017. Use of Pig as a Model for Mesenchymal Stem Cell Therapies for Bone Regeneration. Anim Biotechnol. 28:275-287.   DOI
2 Singh VK, Thrall KD, Hauer-Jensen M. 2016. Minipigs as models in drug discovery. Expert Opin Drug Discov. 11:1131-1134.   DOI
3 Siriboon C, Lin YH, Kere M, Chen CD, Chen LR, Chen CH, Tu CF, Lo NW, Ju JC. 2015. Putative porcine embryonic stem cell lines derived from aggregated four-celled cloned embryos produced by oocyte bisection cloning. PLoS One. 10:e0118165.   DOI
4 Son HY, Kim JE, Lee SG, Kim HS, Lee E, Park JK, Ka H, Kim HJ, Lee CK. 2009. Efficient Derivation and Long Term Maintenance of Pluripotent Porcine Embryonic Stem-like Cells. Asian-Australas J Anim Sci. 22:26-34.   DOI
5 Telugu BP, Ezashi T, Sinha S, Alexenko AP, Spate L, Prather RS, Roberts RM. 2011. Leukemia inhibitory factor (LIF)-dependent, pluripotent stem cells established from inner cell mass of porcine embryos. J Biol Chem. 286:28948-28953.   DOI
6 Vackova I, Ungrova A, Lopes F. 2007. Putative embryonic stem cell lines from pig embryos. J Reprod Dev. 53:1137-1149.   DOI
7 Walters EM and Prather RS. 2013. Advancing swine models for human health and diseases. Mo Med. 110:212-215.
8 Wang WH, Day BN, Wu GM. 2003. How does polyspermy happen in mammalian oocytes? Microsc Res Tech. 61:335-341.   DOI
9 Whyte JJ and Prather RS. 2011. Genetic modifications of pigs for medicine and agriculture. Mol Reprod Dev. 78:879-891.   DOI
10 Xue B, Li Y, He Y, Wei R, Sun R, Yin Z, Bou G, Liu Z. 2016. Porcine Pluripotent Stem Cells Derived from IVF Embryos Contribute to Chimeric Development In Vivo. PLoS One. 11:e0151737.   DOI
11 Cheong SA, Kim E, Kwak SS, Jeon Y, Hyun SH. 2015. Improvement in the blastocyst quality and efficiency of putative embryonic stem cell line derivation from porcine embryos produced in vitro using a novel culturing system. Mol Med Rep. 12:2140-2148.   DOI
12 Yamanaka K, Sugimura S, Wakai T, Kawahara M, Sato E. 2009. Difference in sensitivity to culture condition between in vitro fertilized and somatic cell nuclear transfer embryos in pigs. J Reprod Dev. 55:299-304.   DOI
13 Yoshioka K, Suzuki C, Tanaka A, Anas IM, Iwamura S. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol Reprod. 66:112-119.   DOI
14 Baek S, Han NR, Yun JI, Hwang JY, Kim M, Park CK, Lee E, Lee ST. 2017. Effects of Culture Dimensions on Maintenance of Porcine Inner Cell Mass-Derived Cell Self-Renewal. Mol Cells. 40:117-122.   DOI
15 Bogliotti YS, Wu J, Vilarino M, Okamura D, Soto DA, Zhong C, Sakurai M, Sampaio RV, Suzuki K, Izpisua Belmonte JC, Ross PJ. 2018. Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts. Proc Natl Acad Sci USA. 115:2090-2095.   DOI
16 Cha HJ, Yun JI, Han NR, Kim HY, Baek S, Lee SH, Lee J, Lee E, Park CK, Lee ST. 2018. Generation of embryonic stem-like cells from in vivo-derived porcine blastocysts at a low concentration of basic fibroblast growth factor. Reprod Domest Anim. 53:176-185.   DOI
17 Fischer K, Kraner-Scheiber S, Petersen B, Rieblinger B, Buermann A, Flisikowska T, Flisikowski K, Christan S, Edlinger M, Baars W, Kurome M, Zakhartchenko V, Kessler B, Plotzki E, Szczerbal I, Switonski M, Denner J, Wolf E, Schwinzer R, Niemann H, Kind A, Schnieke A. 2016. Efficient production of multi-modified pigs for xenotransplantation by 'combineering', gene stacking and gene editing. Sci Rep. 6:29081.   DOI
18 Hou DR, Jin Y, Nie XW, Zhang ML, Ta N, Zhao LH, Yang N, Chen Y, Wu ZQ, Jiang HB, Li YR, Sun QY, Dai YF, Li RF. 2016. Derivation of Porcine Embryonic Stem-Like Cells from In Vitro-Produced Blastocyst-Stage Embryos. Sci Rep. 6:25838.   DOI
19 Hall VJ, Christensen J, Gao Y, Schmidt MH, Hyttel P. 2009. Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development. Dev Dyn. 238:2014-2024.   DOI
20 Hall VJ and Hyttel P. 2014. Breaking down pluripotency in the porcine embryo reveals both a premature and reticent stem cell state in the inner cell mass and unique expression profiles of the naive and primed stem cell states. Stem Cells Dev. 23:2030-2045.   DOI
21 Jung SK, Kim HJ, Kim CL, Lee JH, You JY, Lee ES, Lim JM, Yun SJ, Song JY, Cha SH. 2014. Enhancing effects of serum-rich and cytokine-supplemented culture conditions on developing blastocysts and deriving porcine parthenogenetic embryonic stem cells. J Vet Sci. 15:519-528.   DOI
22 Lee SG, Park JK, Choi KH, Son HY, Lee CK. 2015. Embryo Aggregation Promotes Derivation Efficiency of Outgrowths from Porcine Blastocysts. Asian-Australas J Anim Sci. 28:1565-1572.   DOI
23 Lee Y, Lee H, Park B, Elahi F, Lee J, Lee ST, Park CK, Hyun SH, Lee E. 2016. Alpha-linolenic acid treatment during oocyte maturation enhances embryonic development by influencing mitogen-activated protein kinase activity and intraoocyte glutathione content in pigs. J Anim Sci. 94:3255-3263.   DOI
24 Mujibi FD, Okoth E, Cheruiyot EK, Onzere C, Bishop RP, Fevre EM, Thomas L, Masembe C, Plastow G, Rothschild M. 2018. Genetic diversity, breed composition and admixture of Kenyan domestic pigs. PLoS One. 13:e0190080.   DOI
25 Perleberg C, Kind A, Schnieke A. 2018. Genetically engineered pigs as models for human disease. Dis Model Mech. 11:dmm030783.   DOI