• Title/Summary/Keyword: hierarchical cluster analysis (HCA)

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Monthly Fish Species Composition Caught by Fyke Net in Eastern Coast Yeosu, the South Sea of Korea (남해 여수 동부연안 각망에 어획된 어류의 종조성 및 월별 출현 양상)

  • Sang-Hun Cha;Tae-Sik Yu;Kyeong-Ho Han;Sung-Hoon Lee
    • Korean Journal of Ichthyology
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    • v.36 no.2
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    • pp.174-181
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    • 2024
  • This study examines the community characteristics of fish species collected by fyke net from the eastern coastal waters of Yeosu, Korea, during the period from 2020 to 2021. A total of 4,539 fish were sampled and classified into 43 species, 28 families, and 7 orders. The dominant species included Mugil cephalus (14.4%), Konosirus punctatus (10.9%), Lateolabrax japonicus (8.3%), Acanthopagrus schlegelii (7.9%), and Pennahia argentata (7.2%). The diversity index was highest in October (H'=2.888) and lowest in February (H'=2.075). Furthermore, the dominance index was highest in March (DI=0.524) and lowest in December (DI=0.178). Hierarchical cluster analysis (HCA) revealed that fish communities could be divided into three groups based on Bray-Curtis similarity. Group 1, consisting mainly of species collected from June to October, formed one cluster. Group 2, including winter spawning species such as Pseudopleuronectes yokohamae, Clupea pallasii, and Hexagrammos otakii, sampled from November to February, formed another cluster. Group 3, which included species such as Larimichthys polyactis, Amblychaeturichthys hexanema, and Sebastes inermis, formed a cluster collected from March to May. The eastern coast of Yeosu is considered a suitable habitat for fisheries resources, including highly economically valuable species, and it is also assumed to be an important spawning and nursery ground for many fish species.

Impurity profiling and chemometric analysis of methamphetamine seizures in Korea

  • Shin, Dong Won;Ko, Beom Jun;Cheong, Jae Chul;Lee, Wonho;Kim, Suhkmann;Kim, Jin Young
    • Analytical Science and Technology
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    • v.33 no.2
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    • pp.98-107
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    • 2020
  • Methamphetamine (MA) is currently the most abused illicit drug in Korea. MA is produced by chemical synthesis, and the final target drug that is produced contains small amounts of the precursor chemicals, intermediates, and by-products. To identify and quantify these trace compounds in MA seizures, a practical and feasible approach for conducting chromatographic fingerprinting with a suite of traditional chemometric methods and recently introduced machine learning approaches was examined. This was achieved using gas chromatography (GC) coupled with a flame ionization detector (FID) and mass spectrometry (MS). Following appropriate examination of all the peaks in 71 samples, 166 impurities were selected as the characteristic components. Unsupervised (principal component analysis (PCA), hierarchical cluster analysis (HCA), and K-means clustering) and supervised (partial least squares-discriminant analysis (PLS-DA), orthogonal partial least squares-discriminant analysis (OPLS-DA), support vector machines (SVM), and deep neural network (DNN) with Keras) chemometric techniques were employed for classifying the 71 MA seizures. The results of the PCA, HCA, K-means clustering, PLS-DA, OPLS-DA, SVM, and DNN methods for quality evaluation were in good agreement. However, the tested MA seizures possessed distinct features, such as chirality, cutting agents, and boiling points. The study indicated that the established qualitative and semi-quantitative methods will be practical and useful analytical tools for characterizing trace compounds in illicit MA seizures. Moreover, they will provide a statistical basis for identifying the synthesis route, sources of supply, trafficking routes, and connections between seizures, which will support drug law enforcement agencies in their effort to eliminate organized MA crime.

Sensory Properties and Drivers of Liking for Pizza Crust (피자 크러스트의 특성과 소비자 기호 유도 인자)

  • Lee, Jisun;Ahn, Sungsoo;Chung, Lana
    • Journal of the Korean Society of Food Culture
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    • v.31 no.6
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    • pp.624-633
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    • 2016
  • This study identified the sensory properties of samples of pizza dough at three pizza companies and three masonry oven pizzerias from Seoul, Korea and compared consumer acceptability among panels of university students. Six pizza dough samples were prepared (pan pizzas from Pizza Hut, Mr.pizza, and Dominos pizza, masonry oven baked pizzas from Appleteen, Mr.Lee's, and Pizza factory). Consumer tests were employed involving 97 Korean consumers. Consumers evaluated overall liking (OL), liking of appearance (APPL), odor (ODL), flavor (FLL), and texture (TXTL), willing to try (WT), and willing to recommend (WR) for the samples using a nine-point hedonic scale. Analysis of variance (ANOVA) indicated that HutP, MrP, and DomP samples had significantly (p<0.05) high scores for roughness, porosity, crust color, grain size, brownness, dairy food aroma, savory taste, and yeast aroma, which had the highest OL, ODL, and FLL scores. LeeP, ATeenP, and PFacP samples had high elasticity, cohesiveness, and adhesiveness. Consumers favored the appearance characteristics and color, dairy product flavor, and savory flavor of the pan pizza and preferred cohesiveness, toughness, and stickiness of masonry oven baked pizza.

Assessment of radioactivity levels and radiation hazards in building materials in Egypt

  • Ahmed E. Abdel Gawad;Mohamed Y. Hanfi;Mostafa N. Tawfik;Mohammed S. Alqahtani;Hamed I. Mira
    • Nuclear Engineering and Technology
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    • v.56 no.2
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    • pp.707-714
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    • 2024
  • Different degrees of natural radioactivity found in quartz can have negative consequences on health. Quartz vein along the investigated Abu Ramad area, Egypt, had its natural radioactivity assessed. The HPGe spectrometer was used to determine the role played by the radionuclides 238U, 232Th, and 40K in the gamma radiation that was emitted, and the results showed that these concentrations are 484.64 ± 288.4, 36.8 ± 13.1 and 772.2 ± 134.6 Bq kg-1 were higher than the corresponding reported global limits of 33, 45, and 412 Bq kg-1 for each radionuclide (238U, 232Th, and 40K). Among the radiological hazard parameters, the excess lifetime cancer risk (ELCR) is estimated and it's mean value of ELCR (1.2) is higher than the permissible limit of 0.00029. The relationship between the radionuclides and the associated radiological hazard characteristics was investigated based on multivariate statistical methods including Pearson correlation, principal component analysis (PCA), and hierarchical cluster analysis (HCA). According to statistical research, the radioactive risk of quartz is primarily caused by the 238U, 232Thand 40K. Finally, applying quartz to building materials would pose a significant risk to the public.

Pattern Recognition of the Herbal Drug, Magnoliae Flos According to their Essential Oil Components

  • Jeong, Eun-Sook;Choi, Kyu-Yeol;Kim, Sun-Chun;Son, In-Seop;Cho, Hwang-Eui;Ahn, Su-Youn;Woo, Mi-Hee;Hong, Jin-Tae;Moon, Dong-Cheul
    • Bulletin of the Korean Chemical Society
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    • v.30 no.5
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    • pp.1121-1126
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    • 2009
  • This paper describes a pattern recognition method of Magnoliae flos based on a gas chromatographic/mass spectrometric (GC/MS) analysis of the essential oil components. The botanical drug is mainly comprised of the four magnolia species (M. denudata, M. biondii, M. kobus, and M. liliflora) in Korea, although some other species are also being dealt with the drug. The GC/MS separation of the volatile components, which was extracted by the simultaneous distillation and extraction (SDE), was performed on a carbowax column (supelcowax 10; 30 m{\time}0.25 mm{\time}0.25{\mu}m$) using temperature programming. Variance in the retention times for all peaks of interests was within RSD 2% for repeated analyses (n = 9). Of the 74 essential oil components identified from the magnolia species, approximately 10 major components, which is $\alpha$-pinene, $\beta$-pinene, sabinene, myrcene, d-limonene, eucarlyptol (1,8-cineol), $\gamma$-terpinene, p-cymene, linalool, $\alpha$-terpineol, were commonly present in the four species. For statistical analysis, the original dataset was reduced to the 13 variables by Fisher criterion and factor analysis (FA). The essential oil patterns were processed by means of the multivariate statistical analysis including hierarchical cluster analysis (HCA), principal component analysis (PCA) and discriminant analysis (DA). All samples were divided into four groups with three principal components by PCA and according to the plant origins by HCA. Thirty-three samples (23 training sets and 10 test samples to be assessed) were correctly classified into the four groups predicted by PCA. This method would provide a practical strategy for assessing the authenticity or quality of the well-known herbal drug, Magnoliae flos.

HPLC-tandem Mass Spectrometric Analysis of the Marker Compounds in Forsythiae Fructus and Multivariate Analysis

  • Cho, Hwang-Eui;Ahn, Su-Youn;Son, In-Seop;Hwang, Gyung-Hwa;Kim, Sun-Chun;Woo, Mi-Hee;Lee, Seung-Ho;Son, Jong-Keun;Hong, Jin-Tae;Moon, Dong-Cheul
    • Natural Product Sciences
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    • v.17 no.2
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    • pp.147-159
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    • 2011
  • A high-performance liquid chromatography-electrospray ionization-tandem mass spectrometric method was developed to determine simultaneously eight marker constituents of Forsythiae fructus, and subsequently applied it to classify its two botanical origins. The marker compounds of Forsythia suspensa were phillyrin, pinoresinol, phillygenin, lariciresinol and forsythiaside; those of F.viridissima were arctiin, arctigenin and matairesinol. Separation of the eight analytes was achieved on a phenyl-hexyl column (150${\times}$2.0 mm i.d., 3 ${\mu}M$) using gradient elution with the mobile phase: (A) 10% acetonitrile in 0.5% acetic acid, (B) 40% aqueous acetonitrile. A few fragment ions specific to the types of lignans, among the product ions generated by collisonally induced dissociation (CID) of molecular ion clusters, such as [M-H]$^-$ or [M+OAc]$^-$ were used not only for fingerprinting analysis but for the quantification of each epimer by using multiple-reaction monitoring mode. It was shown good linearity ($r^2{\geq}$ 0.9998) over the wide range of all analytes; intra- and inter-day precisions (RSD, %) were within 9.14% and the accuracy ranged from 84.3 to 115.1%. The analytical results of 40 drug samples, combined with multivariate statistical analyses - principal component analysis (PCA) and hierarchical cluster analysis (HCA) - clearly demonstrated the classification of the test samples according to their botanical origins. This method would provide a practical strategy for assessing the authenticity or quality of the herbal drug.

Species composition of the catches collected by trammel net in the coastal waters off Ulleungdo of Korea (울릉도해역에서 삼중자망에 의한 어획물의 종조성)

  • CHUNG, Sangdeok;CHA, Hyung Kee;LEE, Jae Bong;LEE, Hae Won;YANG, Jae Hyeong
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.51 no.4
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    • pp.567-575
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    • 2015
  • Species composition in the coastal waters off Ulleungdo of Korea were examined based on catches bimonthly collected by trammel net in 2013. A total of 711 individuals and 181.9 Kg were caught and catches were composed of 4 classes 15 orders 27 families 52 species including 44 Pisces, 4 Gastropoda, 3 Cephalopoda, and 1 Echinodermata. The dominant species in biomass were File fish (Thamnaconus modestus), Atka mackerel (Pleurogrammus azonus), and Greenling (Hexagrammos otakii). Data were summarized using hierarchical cluster analysis (HCA) and detrended correspondence analysis (DCA) to examine similarity in species composition for each month, and community structure in Ulleungdo was divided into two groups. Community structures in February, April and December with low temperature and well-mixed surface water were distinguished from those in June, August and October with high temperature and strong stratification, which could be attributed to temporal changes in dominant species. Atka mackerel and Spear squid mainly caught in February and April, disappearing in June, August and October, and File fish outburst was shown in October. Because the water off Ulleungdo has been under low human pressure, it could be a good case study to elucidate effects of climate change on community structure and ecosystem in the East sea. Continuous surveys and further studies are required to demonstrate migration route and distribution of dominant species and long-term changes in community structure in the water of Ulleungdo.

Comparison of Benthic Macroinvertebrate Communities at Two Headwater Streams Located with Different Temperature Regions in South Korea (온도 분포가 다른 두 산림 하천의 저서성대형무척추동물 군집 특성 비교)

  • Lee, Da-Yeong;Lee, Dae-Seong;Park, Chanwoo;Yun, Soon Jin;Lim, Jong-Hwan;Park, Young-Seuk
    • Korean Journal of Ecology and Environment
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    • v.54 no.2
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    • pp.87-95
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    • 2021
  • Macroinvertebrates in forest streams affect the overall health of other streams in the same water system. In this study, we compared differences in the benthic macroinvertebrate community at two headwater streams located at different latitudes in the southern and northern parts of South Korea. We calculated the community temperature index (CTI), which represents the thermal preferences of the benthic communities. Hierarchical cluster analyses (HCA) were conducted to compare the similarities among sampling sites. In addition, we analyzed the relationship between community composition and environmental and community characteristics using non-metric multidimensional scaling (NMDS). Our results showed that CTI was significantly different between the two regions, indicating that these benthic macroinvertebrate communities have different thermal preferences. These two regions were clearly distinguished from each other in the HCA; furthermore, seasonal differences in benthic community composition were observed within each region. The functional feeding groups present in the benthic macroinvertebrate communities were different even though their habitat was similar.

Cancer: Scenario and Relationship of Different Geographical Areas of the Globe with Special Reference to North East-India

  • Sharma, Jagannath Dev;Kalit, Manoj;Nirmolia, Tulika;Saikia, Sidhartha Protim;Sharma, Arpita;Barman, Debanjana
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.8
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    • pp.3721-3729
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    • 2014
  • Background: Cancer is becoming the most important public health burden around the globe. As per the GLOBOCAN 2008 estimates, about 12.7 million cancer cases and 7.6 million cancer deaths were estimated to have occurred in 2008. The burden of cancer cases for India in the year 2020 is calculated to be 1,148,757 (male 534,353; female 614,404) compared to 979,786 in 2010. The pattern of cancer incidence is varying among geographical regions, esophageal cancer for example being high in China, lung cancer in USA, and gallbladder cancer in Chile. The question remains why? Is it due to the diversity in genome pool, food habits, risk factor association and role of genetic susceptibility or some other factors associated with it? In India, the North East (NE)-India region is seeing a marked increase in cancer incidence and deaths, with a very different cancer incidence pattern compared to mainland India. The genome pool of the region is also quite distinct from the rest of India. Northeastern tribes are quite distinct from other groups; they are more closely related to East Asians than to other Indians. In this paper an attempt was made to see whether there is any similarity among the pattern of cancer incidence cases for different sites of NE-India region to South or East-Asia. Materials and Methods: Principal Component Analysis (PCA), Hierarchical Cluster Analysis (HCA), Pearson Correlation coefficient test was assessed to evaluate the linkage of North-East India region to other regions. A p value <0.05 was considered as statistically significant. Results: The results clearly shows that there are similarities in occurrence of cancer incidence patterns for various cancer sites of NE-India with South and East-Asian regions, which may lead to the conclusion that there might be a genetic linkage between these regions.