• 한국식품영양과학회지
• /
• 제21권2호
• /
• pp.136-142
• /
• 1992

냉동 건조한 귤(Citrus sinensis)의 껍질로 부터 methanol과 butanol추출물인 crude bioflavonoids를 건조중량으로 최초 시료대비 약 0.26%의 수율을 얻을수 있었다. 이 crude bioflavonoids를 gel filtration, HPLC를 이용하여 분리, 정제하였고 TLC, HPLC, UV spectrum, NMR spectral analysis를 통해 narirutin과 hesperidin으로 동정할 수 있었다. 또한 HPLC 정량분석 결과, narirutin과 hesperidin은 crude bioflavonoids의 g당 42mg과 530mg을 얻을 수 있었으며 귤 껍질의 주요 bioflavonoid는 hesperidin으로 판명되었다. 한편 실험 동물인 Sprague-Dawley종의 흰쥐를 대상으로 혈압 변화를 측정한 결과 귤 껍질의 주요 bioflavonoid성분인 hesperidin은 약물 투여 후 유의성있게 (p< 0.001) 혈압 저하효과가 있음을 관찰 할 수 있었다.

• Advances in Traditional Medicine
• /
• 제9권4호
• /
• pp.292-302
• /
• 2009

Ischemia/reperfusion injury, which is commonly seen in the field of renal surgery or transplantation, is a major cause of acute renal failure. Previous studies showed that antioxidant treatments attenuated renal ischemia/reperfusion injury. The objective of this study was to examine the role of hesperidin in modulating reactive oxygen species induced inflammation and apoptosis after renal ischemia/reperfusion injury. Rats were subjected to right nephrectomy, 15 days later 45 min of renal ischemia and 24 h reperfusion with or without treatment with hesperidin. Renal function, inflammation and apoptosis were compared at 24 h after reperfusion injury. Hesperidin improved the renal dysfunction and reduced inflammation and apoptosis after ischemia/reperfusion injury. In conclusion, hesperidin shows potent anti-apoptotic and antiinflammatory properties due to antioxidant property. These findings may have major implications in the treatment of human ischemic acute renal failure.

• Preventive Nutrition and Food Science
• /
• 제11권4호
• /
• pp.289-297
• /
• 2006

This study examined the effect of hesperidin supplementation with an ethanol diet on lipid and antioxidant metabolism in rats. Male Sprague-Dawley rats were divided into two groups (n=10), and were assigned to one of two dietary categories: $E_8$, ethanol diet (50 g/L) for 8 wks; $E_8H_4$, ethanol diet for the first 4 wks and hesperidin (0.02%, w/w) supplemented ethanol diet for the last 4 wks. The plasma and hepatic lipids, hepatic cholesterol regulating enzyme activity, hepatic antioxidant enzyme activity and lipid peroxidation were determined. Supplementation with hesperidin for the last 4 wks during the 8 wks period of the ethanol diet, significantly increased the ADH activity. In conjunction with the chronic administration of ethanol, hesperidin supplementation resulted in a significant decrease in the hepatic cholesterol and triglyceride concentrations compared to the $E_8$ group. The hepatic HMG-CoA reductase and ACAT activities were significantly lower in the hesperidin-supplemented group. When comparing hepatic antioxidant enzyme activities, SOD, GSH-Px, and G6PD activities and GSH level were significantly higher in the $E_8H_4$ group than in the E8 group. Plasma TBARS levels were significantly lower in rats fed ethanol with hesperidin compared to the rats fed only ethanol; however, the hepatic TBARS levels were not significantly different between the groups. Accordingly, the additional hesperidin supplement with an ethanol diet might be effective for improving the hepatic lipid metabolism and antioxidant defense system.

• Natural Product Sciences
• /
• 제21권2호
• /
• pp.141-145
• /
• 2015

Hesperidin, which is the most abundant flavonoid of Citrus unshiu (Rutaceae), has been reported to possess diverse activities and widely used as functional foods and cosmetics. For the development of functional products, extraction procedure is indispensable. Extraction conditions affect the composition of extract as well as its biological activity. Therefore, we tried to optimize extraction conditions such as extraction solvent, extraction time and extraction temperature for maximum yield of hesperidin using response surface methodology with threelevel-three-factor Box-Behnken design (BBD). Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as ethanol concentration, 59.0%; temperature $71.5^{\circ}C$ and extraction time, 12.4 h. The hesperidin yield under the optimal condition was found to be $287.8{\mu}g$ per 5 mg extract, which was well matched with the predicted value of 290.5 μg. These results provides optimized extraction condition for hesperidin and might be useful for the development of hesperidin as functional products like health supplements, cosmetics and medicinal products.

• Biomolecules & Therapeutics
• /
• 제24권3호
• /
• pp.312-319
• /
• 2016

Human skin cells undergo pathophysiological processes via generation of reactive oxygen species (ROS) upon excessive exposure to ultraviolet B (UVB) radiation. This study investigated the ability of hesperidin ($C_{28}H_{34}O_{15}$) to prevent apoptosis due to oxidative stress generated through UVB-induced ROS. Hesperidin significantly scavenged ROS generated by UVB radiation, attenuated the oxidation of cellular macromolecules, established mitochondrial membrane polarization, and prevented the release of cytochrome c into the cytosol. Hesperidin downregulated expression of caspase-9, caspase-3, and Bcl-2-associated X protein, and upregulated expression of B-cell lymphoma 2. Hesperidin absorbed wavelengths of light within the UVB range. In summary, hesperidin shielded human keratinocytes from UVB radiation-induced damage and apoptosis via its antioxidant and UVB absorption properties.

• 한국식품과학회지
• /
• 제34권1호
• /
• pp.132-135
• /
• 2002

제주재래종 감귤류의 9종의 미숙과의 naringin, hesperidin, neohesperidin 함량 변화를 분석하고 원료 소재로서의 활용가능성을 검토하고자 하였다. Neohesperidin과 naringin 함량은 '당유자'와 '지각'에서 높았고, hesperidin 함량은 '빈귤'과 '동정귤'에서 높았으나 모두 현재 이들 flavonoid 소재의 상업적 추출에 이용되고 있는 품종들에 비하면 낮았다. 그러나 본 실험에서 수확을 시작한 초기에 각 flavonoid 함량의 급격한 감소가 일어남에 따라 경쟁력 있는 소재의 선발을 위해서는 보다 이른 시기의 미숙과에 대한 검토가 필요하며 특히 '당유자' 및 '지각'의 neohesperidin 및 naringin 소재로서의 활용가능성이 높은 것으로 평가되었다.

• Animal cells and systems
• /
• 제13권4호
• /
• pp.357-362
• /
• 2009

Hesperidin, the most abundant polyphenolic compound found in citrus fruits, has been known to possess neuroprotective, sedative, and anticonvulsive effects on the nervous system. In a recent electrophysiological study, it was reported that hesperidin induced biphasic change in population spike amplitude in hippocampal CA1 neurons in response to both single spike stimuli and theta-burst stimulation depending on its concentration. However, the precise mechanism by which hesperidin acts on neuronal functions has not been fully elucidated. Here, using whole-cell patch-clamp recording, we revealed that hesperidin did not affect excitatory synaptic activities such as basal synaptic transmission and theta-burst LTP. Moreover, in a current injection experiment, spike number, resting membrane potential and action potential threshold also remained unchanged. Taken together, these results indicate that the effects of hesperidin on the neuronal functions such as spiking activity might not be attributable to either modification of excitatory synaptic transmissions or changes in membrane excitability in hippocampal CA1 neuron.

• 분석과학
• /
• 제14권4호
• /
• pp.340-348
• /
• 2001

감귤류와 한국산 청피에 함유된 glycosided flavonoids (naringenin, naringin, narirutin, hesperidin, hesperetin)를 HPLC, UV, 그리고 NMR로 분리하고 분석하였다. Glycosided flavonoids의 함량을 감귤종류와 성숙도에 따라 비교하였다. 청피의 주성분을 hesperidin이었고, narirutin, hesperetin도 상당량 들어있었다. 지실의 주성분은 naringin, narirutin, naringenin이었고, 지각의 주성분은 narirutin, hesperidin이었다. 밀감과 오렌지의 주성분은 narirutin, hesperidin이었으며, glycosided flabonoids의 함량은 감귤의 성숙도에 따라 감소하였다.

• 생물환경조절학회지
• /
• 제22권1호
• /
• pp.55-59
• /
• 2013

본 연구는 레몬과즙의 헤스페리딘 함량이 가장 많은 수확시기 지표를 결정하기 위해 수관내부 및 외부에 착과된 과실에 대해 시기별 헤스페리딘 함량을 착색시기 및 과중과 연관시켜 평가했다. 그 함량은 과실생장에 따라 달랐으며 수관내부 및 외부에 있어 착색이 막 시작되는 개화 후 162일과 176일째에 가장 많았다. 그리고 수관외부가 내부보다 많았다. 이상의 결과는 레몬과실의 헤스페리딘 함량이 가장 많은 적정 수확 시기는 착색이 막 시작이 되고 과실의 생장이 멈추기 직전이라는 것을 나타냈다.

• 한국식품저장유통학회지
• /
• 제19권5호
• /
• pp.652-658
• /
• 2012

수확시기에 따른 제주산 가공용 감귤의 이화학적 특성과 hesperidin함량을 검토하였다. 수확시기가 늦어짐에 따라 가용성 고형물의 함량이 증가되는 경향을 보였으며, 당산비도 증가하는 경향을 보였다. 이는 pH의 변화와 비슷한 경향을 보였다. 그러나, 적정산도는 약간 감소하는 경향을 보였다. 색도를 측정한 결과, L, b값과 탁도는 수확시기가 늦어질수록 약간 감소하는 경향을 보였으나, a값은 수확시기가 늦어질수록 약간 증가하는 경향을 보였다. 백탁현상의 주요원인이 되는 hesperidin함량은 수확시기가 늦어짐에 따라 감소하는 경향을 보였다. 이는 빠르게 수확한 과즙을 이용하여 가공품을 제조 시 혼탁이 적을 것으로 예상된다.