• 한국식품저장유통학회지
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• 제24권4호
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• pp.542-549
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• 2017

In this study, a peptide exhibiting antioxidant activity was isolated from sandfish (Arctoscopus japonicus) roe hydrolysate (SRH) in order to evaluate their practical uses as materials for manufacturing functional foods. The A. japonicus roe protein was hydrolyzed using Collupulin MG, and isolation of antioxidant peptide was performed using ultrafiltration (UF), prep-HPLC, and RP-HPLC. The SRH with a molecular weight below 3 kDa constituted about 38% of the whole hydrolysate, and the fraction with a molecular weight below 3 kDa showed significantly greater antioxidant activity compared to the original SRH and other fractions. The isolation fold of the antioxidant peptide isolated from SRH throughout the four-step procedure was 7.11-fold, and protein yield was 14.8%. The DPPH radical scavenging activity of isolated antioxidant peptide was above 90% at a concentration of 1.0 mg/mL, which was similar to that of the Trolox at a concentration of 0.1 mg/mL. These results suggested that the antioxidant peptide derived from A. japonicus roe could be a useful additive for producing functional foods and protein supplements. However, it is necessary to perform further study the structural characteristics of this antioxidant peptide isolated from A. japonicus roe.

• Journal of Applied Biological Chemistry
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• 제62권3호
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• pp.265-273
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• 2019

This study aimed to evaluate the anti-atherosclerotic and anti-hypertensive effects of six different plant extracts using a N(G)-nitro-L-arginine-methyl ester (L-NAME)-induced rat model of hypertension. All extracts were administered orally for six weeks. At the end of the study period blood pressure, blood flow, aortic histopathology, and hepatic endothelial nitric oxide synthase (eNOS) expression were measured. Subsequently, we also measured the levels of intracellular reactive oxygen species, nitric oxide (NO), and anti-inflammatory cytokines in vitro. Based on these screening results, we selected extracts of Cinnamomum cassia (C. cassia) and Salvia miltiorrhiza (S. miltiorrhiza) for further evaluation. C. cassia and S. miltiorrhiza extracts ameliorated hypertension and atherosclerosis in L-NAME-treated rats in a dose-dependent manner. In addition, a mixture of C. cassia and S. miltiorrhiza had an additive effect to reduce blood pressure, increase blood flow, and normalize aortic tissue. This mixture demonstrated anti-oxidative and anti-inflammatory activities in vitro. In conclusion, although further analysis of the therapeutic mechanism is required, the anti-hypertensive and anti-atherosclerotic effects of this mixture are likely mediated by increased eNOS expression, and its anti-oxidative and anti-inflammatory activities.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2020년도 정기학술대회 발표논문집
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• pp.221-221
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• 2020

Curcumin, a hydrophobic polyphenol derived from turmeric, has been used a food additive and as a herbal medicine for the treatment of various diseases. In the present study, we found the functional role of a nanosphere loaded with curcumin (CN) in the promotion of the motility of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) during the wound closure. We found that the efficacy of hUCB-MSCs migration induced by CN was 1000-fold higher than that of curcumin powder. CN significantly increased the motility of hUCB-MSCs by activating c-Src, which is responsible for the phosphorylation of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK). CN induced the expression levels of α-actinin-1, profilin-1 and filamentous-actin, as regulated by the phosphorylation of nuclear factor-kappa B during its promotion of cell migration. In a mouse skin excisional wound model, we found that transplantation of UCB-MSCs pre-treated with CN enhances wound closure, granulation, and re-epithelialization at mouse skin wound sites. These results indicate that CN is a functional agent that promotes the mobilization of UCB-MSCs for cutaneous wound repair.

• 생명과학회지
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• 제27권4호
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• pp.417-422
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• 2017

본 연구는 천연물 소재인 정금나무 열매(Vaccinum oldhami fruit) 에탄올 추출물을 이용하여 항염증 효과에 대하여 검증하였다. 대식세포인 RAW 264.7 세포를 이용하여 정금나무 열매 추출물로 처리했을 때 세포 생존율을 확인한 결과 $500{\mu}g/ml$ 농도에서 118%를 나타내었다. 대식세포를 이용하여 정금나무 열매 추출물의 농도가 증가함에 따라서 변화하는 NO 생성량을 측정한 결과 최고농도 $1,000{\mu}g/ml$에서 47.3%로 감소한 것을 확인할 수 있었다. 정금나무 열매 에탄올 추출물을 Western blot을 이용하여 단백질 발현을 측정한 결과 처리한 세포군에서 농도가 증가함에 따라 iNOS와 COX-2의 단백질 발현양이 감소하여 최고 농도인 $500{\mu}g/ml$에서 각각 36.13%, 29.61%의 발현 억제를 보여주었다. Reverse-transcription PCR을 통하여 mRNA 발현양을 측정한 결과 iNOS와 COX-2의 mRNA 발현양이 감소하여 $500{\mu}g/ml$ 농도에서 62.25%, 90.07%로 내었다. 이러한 결과로 보아 정금나무 열매 에탄올 추출물의 항염증에 관한 그 효능을 확인할 수 있었고, 따라서 정금나무 열매 추출물을 이용한 천연 항염증 화장품 소재개발 이용 가능성을 넓힐 수 있을 것으로 사료된다.

• 한국식품저장유통학회지
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• 제19권1호
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• pp.12-18
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• 2012

본 연구는 유근피 물 추출물을 각각 0%, 20%, 40%, 60%, 80%, 100% 첨가한 유근피 멥쌀식혜 및 찹쌀식혜의 $4^{\circ}C$에서 15일간, $25^{\circ}C$에서 7일간 저장하면서 저장성 및 관능 특성을 검토하였으며 그 결과는 다음과 같다. 유근피 멥쌀식혜 및 찹쌀식혜의 저장 중 pH 및 산도변화는 유근피 추출물의 첨가량이 적을수록 pH가 낮았고 산도는 높았으며, 저장 기간이 길어질수록 계속 pH는 저하되었고 산도는 높아졌다. 유근피 멥쌀식혜와 찹쌀식혜의 저장 중 총균수는 $4^{\circ}C$의 경우 제조 당일의 균수는 4.6~5.0 Log CFU/g이었으며 저장 7일까지 0%첨가구의 경우 8.8~9.0 Log CFU/g까지 증가하였으나 유근피추출물 80% 및 100% 첨가한 경우는 7.8 Log CFU/g 및 6.9 Log CFU/g로 유근피 추출물의 첨가량이 많을수록 총균수 증가가 적었다. 0%~60% 유근피 첨가식혜의 경우 저장기간 7일 까지 총균수가 최대에 도달하여 그 이후로는 변화가 없었으나 80% 및 100% 첨가구의 경우는 10~13일에 균수가 최대에 도달하는 결과로 유근피에 의하여 저장기간이 연장됨을 알 수 있었다. $25^{\circ}C$의 경우 총균수는 제조 당일 4.6~5.0 Log CFU/g에서 저장기간동안 계속 균수가 증가하여 저장 7일째에는 8.9~9.5 Log CFU/g까지 늘어났으며 유근피 추출물의 첨가량에 따른 차이는 없었다. 유근피 멥쌀식혜 및 찹쌀식혜의 관능적 특성은 유근피 멥쌀식혜는 전체적인 기호도에서 20%첨가구가 $4.23{\pm}0.95$로 가장 높았으며, 유근피 찹쌀식혜는 전체적인 기호도에서 40% 첨가구가 $3.95{\pm}0.95$로 가장 높았다. 20%, 40%의 유근피추출액을 함유한 식혜에서 맛, 향, 단맛, 종합적 기호도에서 유의적으로 높은 값을 보였다(p<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제20권9호
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• pp.1453-1461
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• 2007

This study was conducted to test the hypothesis that dietary supplementation with an herbal extract of Acanthopanax senticosus (AS) enhances the immune response in weaned piglets. Sixty piglets weaned at 21 days of age were randomly assigned to 3 treatment groups representing the addition of 0 or 1 g/kg of the AS extract or 0.2 g/kg of colistin (an antibiotic) to maize- and soybean meal-based diets (n = 20 per group). On days 7, 14 and 28 after initiation of the addition, total and differential counts of leucocytes, proliferating activity of peripheral lymphocytes, serum levels of immunoglobulins (Ig) and cytokines and the spleen index were determined. The AS extract decreased (p<0.05) the number of neutrophils on days 7 and 28 in comparison with the control group and reduced (p<0.05) serum interleukin-$1{\beta}$ level on day 28 compared with the other 2 groups. Dietary supplementation with the AS extract increased (p<0.05) the lymphocyte/leukocyte ratio on day 28 compared with the control group and increased the proliferating activity of lymphocytes on days 14 and 28 compared with the other 2 groups. The AS extract increased (p<0.05) the serum content of IgG on day 7 and of IgG and IgM on day 28 compared with the other 2 groups, as well as increasing the serum content of tumor necrosis factor on day 7 and spleen index on days 7 and 28 compared with the control group. Collectively, these findings suggest that the AS extract as a dietary additive enhances the cellular and humoral immune responses of weaned piglets by modulating the production of immunocytes, cytokines and antibodies.

• 대한한의학방제학회지
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• 제19권1호
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• pp.35-49
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• 2011

Objectives : This study aims to reveal the characteristics of formulas in "Xi$\v{a}$o' Er Y$\`{a}$o Zh$\`{e}$ng Zh$\'{\i}$ Ju$\'{\e}$. Methods : For that objectives, We analyzes formulas in "Xi$\v{a}$o' Er Y$\`{a}$o Zh$\`{e}$ng Zh$\'{\i}$ Ju$\'{\e}$. In the text, 132 formulas were described. To comprehend the formulas, we classified them as several bases. Results : After those analyses, we bring to a conclusion as follows. 1. 30 formulas are described that treated convulsive diseases (j$\={i}$ngf$\={e}$ng, 惊風). Next, g$\={a}$n(疳), parasite infection, diarrhea/dysentery, dermatosis and etc were in the order. 2. Classified by the formulation, Yu$\'{\a}$nj$\`{i}$(圓劑) was the best(70 kinds of formulas, 53%). S$\v{a}$nj$\`{i}$(散劑) was a form of 41 formulas(31%). T$\={a}$ngj$\`{i}$(湯劑) and g$\={a}$oj$\`{i}$(膏劑) were a form of 5 formulas each. 10 formulas were assumed the form of w$\`{a}$iy$\`{o}$ngj$\`{i}$(外用劑). 3. We researched in-depth analysis of Yu$\'{\a}$nj$\`{i}$. As a results, dosage, additive(輔料) and the time to take of Yu$\'{\a}$nj$\`{i}$ were decomposed. Also, the formulas that treated convulsive diseases were analyzed by the herbs classification. Conclusions : Though the formulas that treated convulsive diseases were hard to application at local clinic, overall nosology of pediatrics was reflected comparatively. "Xi$\v{a}$o' Er Y$\`{a}$o Zh$\`{e}$ng Zh$\'{\i}$ Ju$\'{\e}$ was expected to play a role for reconsideration of formulas' formulation.

• 대한한방내과학회지
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• 제24권2호
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• pp.220-232
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• 2003

Objective : The purpose of this study was to analyze the effects of HwangRyunHaeDok-Tang and combinations of constituent herbs on the arterial contraction. Methods : In order to investigate the effects Scutellariae Radix. Coptidis Rhizoma, Phellodendri Cortex and Gardeniae Fructus, in which one of them, two of them, and all of them, were used to exam. Results : The results were summarized as follows; 1. HwangRyunHaeDok-Tang significantly inhibited the contraction of artery induced by Norepinephrine(NE). However the atonic effect was slightly blunted when the vascular endothelial cell was removed. No significant change in the atonic effect of HwangRyunHaeDok-Tang was found when $_L-NNA$ was used as a preliminary treatment. These results indicate that the vascular atonic effect by HwangRyunHaeDok-Tang is slightly dependent on the endothelial cell, and that the HwangRyunHaeDok-Tang works directly to the vascular smooth muscle in creating the vascular atonic effect. 2. The pretreatment of HwangRyunHaeDok-Tang extract significantly inhibited the contractile response to additive application of $Ca^{2+}$ in the strips which were contracted by NE in $Ca^{2+}$-free solution. 3. HwangRyunHaeDok-Tang extract increased the contraction of arterial smooth muscle induced by KCl. Therefore, it can be concluded that HwangRyunHaeDok-Tang may block the NE-receptor or receptor-operated $Ca^{2+}$ channel. 4. It was determined that Scutellariae Radix, Coptidis Rhizoma and Phellodendri Cortex among the ingredients of HwangRyunHaeDok-Tang have a vascular atonic effect. In addition, those ingredients plays a role in strengthening the atonic effect by working with other herbal medicines. Gardeniae Fructus causes the blood vessel to contract. but it does not influence the atonic effects of other herbal medicines. However Gardeniae Fructus tends to inhibit the vascular atonic effect of Phellodendri Cortex. Conclusion : Based on the above results, it can be said that HwangRyunHaeDok-Tang can be applied to cure hypertension considering those three herbs have significant effects of relaxation.

• Natural Product Sciences
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• 제11권1호
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• pp.45-49
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• 2005

Previously, a flavonoid fraction, which consisted mainly of protocatechuic acid, fustin, fisetin, sulfuretin, and butein, here named RCMF [${\underline{R}}hus$ verniciflua Stokes (RVS) ${\underline{c}}hloroform-{\underline{m}}ethanol\;{\underline{f}}raction$], was prepared from a crude acetone extract of RVS which is traditionally used as a food additive and as an herbal medicine. In the present study, we investigated the effects of $TNF-{\alpha}$ on RCMF-induced growth inhibition and apoptosis induction using human osteosarcoma (HOS) cells. The results from tritium uptake and MTT assays showed that $TNF-{\alpha}$ treatment itself (10 ng/ml) did not induce any cytotoxicity, but it actively accelerated RCMF-mediated cytotoxicity of HOS cells. RCMF-induced cytotoxicity and its facilitation by $TNF-{\alpha}$ was verified to be apoptotic, based on the increased DNA fragmentation and low fluorescence intensity in nuclei after propidium iodide (PI) staining of HOS cells. This speculation was further demonstrated by monitoring the Annexin V/PI double staining which could discriminate the difference between apoptotic and necrotic deaths. Collectively, our findings indicate that $TNF-{\alpha}$ accelerates RCMF-induced cytotoxicity in HOS cells.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1562-1568
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• 2013

This study was conducted to evaluate the effects of indigenous herbal supplements on growth, blood metabolites and carcass characteristics in the late fattening period of Hanwoo steers. In a 6 month feeding trial, thirty Hanwoo steers ($647{\pm}32$ kg) were allotted to one of 5 treatment groups, control (basal diet contained lasalocid), licorice, clove, turmeric and silymarin, with six steers per pen. All groups received ad libitum concentrate and 1 kg rice straw/animal/d throughout the feeding trial. Blood samples were collected at the beginning, middle, and the end of the experiment and the steers were slaughtered at the end. Blood glucose, triglyceride, total protein, and albumin concentrations were higher in the turmeric treatment compared with other treatments. Blood urea nitrogen and creatinine concentrations were highest (p<0.003 and p = 0.071, respectively) in steers treated with silymarin. Alanine aminotransferase activity was lower (p<0.06) for licorice and silymarin compared with the control group. There were no alterations in serum aspartate aminotransferase and gamma glutamyltransferase activities as a consequence of herb treatments (p = 0.203 and 0.135, respectively). Final body weight, body weight gain, average dairy gain and dry matter intake were not significantly different among treatments. Yield grade, marbling score and quality grade were higher for silymarin group than those of the control group (p<0.05). Therefore, the results suggest that silymarin can be used an effective dietary supplement as an alternative to antibiotic feed additive and a productivity enhancer, providing safe and more consumer acceptable alternative to synthetic compounds during the late fattening period of steers.