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http://dx.doi.org/10.11002/kjfp.2017.24.4.542

Isolation of antioxidant peptide from sandfish (Arctoscopus japonicus) roe hydrolysate  

Jang, Hye Lim (Department of Food and Nutrition, Yeungnam University)
Shin, Seung Ryeul (Faculty of Herbal Cuisine and Nutrition, Daegu Haany University)
Yoon, Kyung Young (Department of Food and Nutrition, Yeungnam University)
Publication Information
Food Science and Preservation / v.24, no.4, 2017 , pp. 542-549 More about this Journal
Abstract
In this study, a peptide exhibiting antioxidant activity was isolated from sandfish (Arctoscopus japonicus) roe hydrolysate (SRH) in order to evaluate their practical uses as materials for manufacturing functional foods. The A. japonicus roe protein was hydrolyzed using Collupulin MG, and isolation of antioxidant peptide was performed using ultrafiltration (UF), prep-HPLC, and RP-HPLC. The SRH with a molecular weight below 3 kDa constituted about 38% of the whole hydrolysate, and the fraction with a molecular weight below 3 kDa showed significantly greater antioxidant activity compared to the original SRH and other fractions. The isolation fold of the antioxidant peptide isolated from SRH throughout the four-step procedure was 7.11-fold, and protein yield was 14.8%. The DPPH radical scavenging activity of isolated antioxidant peptide was above 90% at a concentration of 1.0 mg/mL, which was similar to that of the Trolox at a concentration of 0.1 mg/mL. These results suggested that the antioxidant peptide derived from A. japonicus roe could be a useful additive for producing functional foods and protein supplements. However, it is necessary to perform further study the structural characteristics of this antioxidant peptide isolated from A. japonicus roe.
Keywords
sandfish; Arctoscopus japonicus; hydrolysate; isolation; antioxidant peptide;
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1 Liu Z, Dong S, Xu J, Zeng M, Song H, Zhao Y (2008) Production of cysteine-rich antimicrobial peptide by digestion of oyster (Crassostrea gigas) with alcalase and bromelin. Food Control, 19, 231-235   DOI
2 Mendis E, Rajapakse N, Kim SK (2005) Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate. J Agric Food Chem, 53, 581-587   DOI
3 Duarte J, Vinderola G, Ritz B, Perdigon G, Matar C (2006) Immunomodulating capacity of commercial fish protein hydrolysate for diet supplementation. Immunobiology, 211, 341-350   DOI
4 Pihlanto-Leppala A (2000) Bioactive peptides derived from bovine whey proteins: opioid and ace-inhibitory peptides. Trends Food Sci Technol, 11, 347-356   DOI
5 Intarasirisawat R, Benjakul S, Wu J, Visessanguan W (2013) Isolation of antioxidative and ACE inhibitory peptides from protein hydrolysate of skipjack (Katsuwana pelamis) roe. J Funct Foods, 5, 1854-1862   DOI
6 Kumar NSS, Nazeer RA, Jaiganesh R (2012) Purification and identification of antioxidant peptides from the skin protein hydrolysate of two marine fishes, horse mackerel (Magalaspis cordyla) and croaker (Otolithes ruber). Amino Acids, 42, 1641-1649   DOI
7 Chi CF, Hu FY, Wang B, Ren XJ, Deng SG, Wu CW (2015) Purification and characterization of three antioxidant peptides from protein hydrolyzate of croceine croaker (Pseudosciaena crocea) muscle. Food Chem, 168, 662-667   DOI
8 Chi CF, Wang B, Hu FY, Wang YM, Zhang B, Deng SG, Wu CW (2015) Purification and identification of three novel antioxidant peptides from protein hydrolysate of bluefin leatherjacket (Navodon septentrionalis) skin. Food Res Int, 73, 124-129   DOI
9 Chi CF, Wang B, Wang YM, Zhang B, Deng SG (2015) Isolation and characterization of three antioxidant peptides from protein hydrolysate of bluefin leatherjacket (Navodon septentrionalis) heads. J Funct Foods, 12, 1-10   DOI
10 Kangsanant S, Thongraung C, Jansakul C, Murkovic M, Seechamnanturakit V (2015) Purification and characterisation of antioxidant and nitric oxide inhibitory peptides from Tilapia (Oreochromis niloticus) protein hydrolysate. Int J Food Sci Technol, 50, 660-665   DOI
11 Kou X, Gao J, Xue Z, Zhang Z, Wang H, Wang X (2013) Purification and identification of antioxidant peptides from chickpea (Cicer arietinum L.) albumin hydrolysates. LWT-Food Sci Technol, 50, 591-598   DOI
12 Song R, Wei RB, Ruan GQ, Luo HY (2015) Isolation and identification of antioxidative peptides from peptic hydrolysates of half-fin anchovy (Setipinna taty). LWT-Food Sci Technol, 60, 221-229   DOI
13 Jang HL, Liceaga AM, Yoon KY (2016) Purification, characterisation and stability of an antioxidant peptide derived from sandfish (Arctoscopus japonicus) protein hydrolysates. J Funct Foods, 20, 433-442   DOI
14 Kang N, Ko SC, Samarakoon K, Kim EA, Kang MC, Lee SC, Kim J, Kim YT, Kim JS, Kim H, Jeon YJ (2013) Purification of antioxidative peptide from peptic hydrolysates of Mideodeok (Styela clava) flesh tissue. Food Sci Biotechnol, 22, 541-547   DOI
15 Zeng WC, Zhang WH, He Q, Shi B (2015) Purification and characterization of a novel antioxidant peptide from bovine hair hydrolysates. Process Biochem, 50, 948-954   DOI
16 Chyung M (1977) The Fishes of Korea. Il Ji Sa, Seoul, Korea, p 727
17 Choi SH, Chun YY, Son SJ, Suh HK (1983) Age, growth and maturity of sandfish, Arctoscopus japonicus (Steindachner ) in the eastern sea of Korea. Bull Nat Fish Res Dev Agency, 31, 7-19
18 Statistics Korea. Index of agriculture, forestry and fishing production. http://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1EW0005&vw_cd= (accessed May 2017)
19 Sathivel S, Smiley S, Prinyawiwatkul W, Bechtel PJ (2005) Functional and nutritional properties of red salmon (Oncorhynchus nerka) enzymatic hydrolysates. J Food Sci, 70, c401-c406   DOI
20 Klompong V, Benjakul S, Kantachote D, Shahidi F (2007) Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chem, 102, 1317-1327   DOI
21 Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem, 150, 76-85   DOI
22 Souissi N, Bougatef A, Triki-Ellouz Y, Nasri M (2007) Biochemical and functional properties of sardinella (Sardinella aurita) by-product hydrolysates. Food Technol Biotechnol, 45, 187-194
23 Ovissipour M, Kenari AA, Motamedzadegan A, Nazari RM (2012) Optimization of enzymatic hydrolysis of visceral waste proteins of yellowfin tuna (Thunnus albacares). Food Bioprocess Technol, 5, 696-705   DOI
24 Bhaskar N, Benila T, Radha C, Lalitha RG (2008) Optimization of enzymatic hydrolysis of visceral waste proteins of Catla (Catla catla) for preparing protein hydrolysate using a commercial protease. Bioresour Technol, 99, 335-343   DOI
25 Bhaskar N, Mahendrakar NS (2008) Protein hydrolysate from visceral waste proteins of Catla (Catla catla): Optimization of hydrolysis conditions for a commercial neutral protease. Bioresour Technol, 99, 4105-4111   DOI
26 Deeslie WD, Cheryan M (1992) Fractionation of soy protein hydrolysates using ultrafiltration membranes. J Food Sci, 57, 411-413   DOI
27 Zhou K, Sun S, Canning C (2012) Production and functional characterisation of antioxidative hydrolysates from corn protein via enzymatic hydrolysis and ultrafiltration. Food Chem, 135, 1192-1197   DOI
28 You L, Zhao M, Regenstein JM, Ren J (2010) Purification and identification of antioxidative peptides from loach (Misgurnus anguillicaudatus) protein hydrolysate by consecutive chromatography and electrospray ionizationmass spectrometry. Food Res Int, 43, 1167-1173   DOI
29 You L, Zhao M, Liu RH, Regenstein JM (2011) Antioxidant and antiproliferative activities of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion. J Agric Food Chem, 59, 7948-7953   DOI
30 Kim SY, Je JY, Kim SK (2007) Purification and characterization of antioxidant peptide from hoki (Johnius belengerii) frame protein by gastrointestinal digestion. J Nutr Biochem, 18, 31-38   DOI
31 Wang JS, Zhao MM, Zhao QZ, Jiang YM (2007) Antioxidant properties of papain hydrolysates of wheat gluten in different oxidation systems. Food Chem, 101, 1658-1663   DOI
32 Cheung LM, Cheung PCK, Ooi VEC (2003) Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem, 81, 249-255   DOI
33 Ranathunga S, Rajapakse N, Kim SK (2006) Purification and characterization of antioxidative peptide derived from muscle of conger eel (Conger myriaster ). Eur Food Res Technol, 222, 310-315   DOI
34 Chen HM, Muramoto K, Yamauchi F, Nokihara K (1996) Antioxidant activity of designed peptides based on the antioxidative peptide isolated from digests of a soybean protein. J Agric Food Chem, 44, 2619-2623   DOI
35 Sheih IC, Fang TJ, Wu TK, Lin PH (2010) Anticancer and antioxidant activities of the peptide fraction from algae protein waste. J Agric Food Chem, 58, 1202-1207   DOI
36 Lee JK, Jeon JK, Byun HG (2011) Effect of angiotensin I converting enzyme inhibitory peptide purified from skate skin hydrolysate. Food Chem, 125, 495-499   DOI