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http://dx.doi.org/10.3839/jabc.2019.036

Anti-atherosclerotic effect of herbal extracts in N(G)-nitro-L-arginine methyl ester-treated rats  

Nagarajan, Senthil (Department of Biology Education, College of Education, Chungbuk National University)
Balamurugan, Rangachari (Department of Biology Education, College of Education, Chungbuk National University)
Shin, Eunju (Univera Co., Ltd.)
Shim, Kyu-Suk (Univera Co., Ltd.)
Kim, Min Jung (Univera Co., Ltd.)
Lee, Jeong Jun (R&D center, Naturetech Co., Ltd.)
Lee, Jae Kwon (Department of Biology Education, College of Education, Chungbuk National University)
Publication Information
Journal of Applied Biological Chemistry / v.62, no.3, 2019 , pp. 265-273 More about this Journal
Abstract
This study aimed to evaluate the anti-atherosclerotic and anti-hypertensive effects of six different plant extracts using a N(G)-nitro-L-arginine-methyl ester (L-NAME)-induced rat model of hypertension. All extracts were administered orally for six weeks. At the end of the study period blood pressure, blood flow, aortic histopathology, and hepatic endothelial nitric oxide synthase (eNOS) expression were measured. Subsequently, we also measured the levels of intracellular reactive oxygen species, nitric oxide (NO), and anti-inflammatory cytokines in vitro. Based on these screening results, we selected extracts of Cinnamomum cassia (C. cassia) and Salvia miltiorrhiza (S. miltiorrhiza) for further evaluation. C. cassia and S. miltiorrhiza extracts ameliorated hypertension and atherosclerosis in L-NAME-treated rats in a dose-dependent manner. In addition, a mixture of C. cassia and S. miltiorrhiza had an additive effect to reduce blood pressure, increase blood flow, and normalize aortic tissue. This mixture demonstrated anti-oxidative and anti-inflammatory activities in vitro. In conclusion, although further analysis of the therapeutic mechanism is required, the anti-hypertensive and anti-atherosclerotic effects of this mixture are likely mediated by increased eNOS expression, and its anti-oxidative and anti-inflammatory activities.
Keywords
Anti-oxidation; Atherosclerosis; Hypertension; Inflammation;
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