• Korean Journal of Microbiology
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• v.44 no.4
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• pp.293-298
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• 2008

HCV is transmitted via various plasma derived products. Current methods to detect hepatitis C virus (HCV) are based on its antibody detection in the donated blood and plasma. Viral contamination can potentially escape such detection during the window period of infection, when no antibody is present or the level of antibody is too low to detect. It is trying to application of nucleic acid amplification tests (NAT) for the direct detection of HCV. The objective of this study was to develop a reliable NAT for the HCV RNA detection from plasma-derived products. The most useful primers was selected for NAT among 5 sets of primers. We have also found that QIAamp viral RNA isolation kit was the most efficient for HCV RNA isolation. The highest sensitivity and specificity was appeared in $48^{\circ}C$ annealing temperature and 30 pmol of primers. With a spiking of HCV to albumin, immunoglobulins and coagulation factors, NAT can detect up to 100 IU/ml. Meanwhile, COBAS amplicor HCV 2.0 afforded a lower sensitivity in high concentrated intramuscular immunoglobulins to below 500 IU/ml. Our results suggested that NAT appears to be a highly sensitive and specific method for HCV RNA detection in plasma-derived products.

• Journal of Korean Home Economics Education Association
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• v.32 no.1
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• pp.89-105
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• 2020

This study aimed at analyzing the clothing management units of technology and home economics textbooks in terms of their contents related to Education for Sustainable Development(ESD). The inquisitive tendency of the textbooks was also examined adopting the modified Romey's method, because it is known as an important factor in increasing student's class participation in sustainable clothing management and inducing practical behavior. First, the learning contents of clothing management unit were analyzed according to the social/political/cultural, environmental, and economic perspectives of ESD. As a result, ESD contents from social/political/cultural perspectives (safety, and health and food), environmental perspectives (natural resources, energy, and environmental problem), and the economic perspectives (sustainable production and consumption) were identified, with a heavy focus on environmental perspective. Secondly, Romey's inquisitive content analysis method was modified to analyze texts, pictures/charts, and learning activities of the clothing management unit. In all textbooks, facts and definitions were the dominant types of contents, which means less opportunities for development of inquiry ability. In conclusion, the ESD contents of the clothing management unit are inclined to the environmental perspective, and it is necessary that textbook development would need to be balanced between three perspectives. Also, future textbook authors will need to improve the inquisitive tendency of the textbooks to encourage student participation in class and induce practical application in real life.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.1
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• pp.1-6
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• 2006

Through the application of a microwave-assisted extraction, the aim of study was to investigate the extraction conditions for the total yield, electron donating ability, total polyphenol contents and nitrite scavenging effect from Pleurotus eryngii. This experiment was conducted under the following extraction conditions: the microwave power (60, 90, 120 W), sample to solvent ratio (1:50, 1:20, 1:10), extraction time (1, 5, 10, 15 mins), and extractant like water, $50\%$ EtOH and $99\%$ EtOH. During the increase of microwave power ranging from 60 to 120 W, the maximized effective components were obtained when the microwave power was set at 60 W. By decreasing the ratio down to 1:10 (g/mL), the extraction of effective components but not total yield was the highest at 1:10 (g/mL). As going up to 15 min, the maximum extraction of effective components was achieved at 1 min for the extraction time and was little affected at over 5 min. In conclusion, the most appropriate conditions for the extraction of effective compounds from Pleurotus eryngii were as follows: the microwave power at 60 W, 1:10 of the sample to solvent ratio, extraction time at 1 min and water as the extractant.

• Science of Emotion and Sensibility
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• v.12 no.2
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• pp.161-168
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• 2009

In this study, we have developed the patch type HAMS (Heart Activity Monitoring System) which is non-restricted, non-awarable and non-invasive. The module using wireless telecommunication to receive the ECG (electrocardiogram) signal at the computer has mobility which it easily monitors the heart activity of subjects in no time for long term at any time and places. We developed the small patch type electrode which can be attached on the chest. Also the reliability and moving artifact of ECG signal measured by this electrode have been verified. Using HAMS, we measured the HRV (Heart Rate Variability) parameters, the questionnaire evaluation for anxiety and stress and the amount of stress hormone (cotisol) to evaluate the stress effect in HRV on the same subject. As a result of comparing the values under non stressed and stressed condition, there was significant difference on many parameters. And the parameter highly related with stress on Pearson's Correlation Coefficient has been examined. These show that using HAMS is able to evaluate the function of autonomic nervous system. Therefore, we can predict heart problem in daily life by using HAMS. Also we expect that this module can be applied for more application as health monitoring system.

• Korean Journal of Environmental Agriculture
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• v.30 no.3
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• pp.339-345
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• 2011

BACKGROUND: Recently in Korea, the import of agricultural products is rising due to the increasing amount of trade. Unregistered pesticides, allidochlor, propachlor, propham, cycloate, diallate and pebulate are widely used as pesticides for rice cultivation in foreign countries, while they are not registered in Korea. Therefore, the residue amount of imported agri-foods should be verified using the proper official analytical method for each of them that has not registered in Korea. METHODS AND RESULTS: This work was conducted to apply the official method of Korea Food & Drug Administration (KFDA) for determining multi class pesticide multiresidues in agricultural commodities. Brown rice and orange which have different characteristics as a matrix were selected as representative samples for residue analysis. The recoveries of cycloate, diallate and pebulate by GC/MS in fortified brown rice and orange with levels of 0.04~0.4 mg/kg were ranged from 82.8% to 110.3%. The quantification limits of three pesticides in brown rice and orange were 0.04 mg/kg. CONCLUSION: As a result, this method can surely be used as an official method for routine analysis of unregistered pesticides in Korea for imported agri-food.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.6
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• pp.4040-4047
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• 2015

The purpose of this study was to analyze a change in Post-traumatic Stress Disorder (PTSD) level and the continuity of the effects in music therapy by applying short-term music therapy program in fire fighters with Post-traumatic Stress (PTS). Forty two fire fighters in 3 districts were randomly assigned to experimental group (21 subjects) and control group (21 subjects). Music therapy was applied in totally 10 sessions by two sessions (120 minutes) a day for 5 days. PTS level was measured immediately after the end of program, after 4 weeks, and after 12 weeks. As a result, PTS level has significantly decreased into $8.90{\pm}4.55$ from $26.52{\pm}2.32$ right after the music therapy program(p<0.05). And the effect was shown to be continued significantly up to after 4 weeks($11.95{\pm}4.57$) and after 12 weeks($13.76{\pm}5.62$)(p<0.05). As a result of research, it is considered that the music therapy is effective approach for reduction in PTS as for fire fighters who belong to the group with high risk of PTSD. Accordingly, the application of regular program for managing fire fighters' mental health is understood to likely to contribute to enhancing the field service competence.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.7
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• pp.3220-3226
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• 2011

In order to monitor internal risk factors such as fire, terror, etc. on the subway station, the surveillance systems using CCTV and various kinds of sensors have been implemented and recently, introduction of surveillance systems using an advanced IT technology, sensor network technology is tried on several areas. Since 2007, Korean government has made an effort to develop the intelligent surveillance and monitoring system, which can monitor fire, intrusion, passenger congestion, health-state of structure, etc., by using wireless sensor network technology and intelligent video analytic technique. For that purpose, this study carried out field wireless communication environment test on Chungmuro Station of Seoul Metro on the basis of ZigBee that is considered as a representative wireless sensor network before field application of the intelligent integrated surveillance system being developed, arranged and analyzed and ZigBee based wireless communication environment test results on the platform and waiting room of Chungmuro Station on this paper. Results of wireless spectrum analysis on the platform and waiting room showed that there is no radio frequency overlapped with that of ZigBee based sensor network and no frequency interference with adjacent frequencies separated 10MHz or more. As results of wireless data transmission test using ZigBee showed that data transmission is influenced by multi-path fading effect from the number and flow rate of passengers on the platform or the waiting room rather than effects from entrance and exit of the train to/from the platform, it should be considered when implementing the intelligent integrated surveillance system on the station.

• Restorative Dentistry and Endodontics
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• v.36 no.2
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• pp.98-107
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• 2011

Caries remains prevalent throughout modern society and is the main disease in the field of dentistry. Although studies of this disease have used diverse methodology, recently, X-ray microtomography has gained popularity as a non-destructive, 3-dimensional (3D) analytical technique, and has several advantages over the conventional methods. According to X-ray source, it is classified as monochromatic or polychromatic with the latter being more widely used due to the high cost of the monochromatic source despite some advantages. The determination of mineral density profiles based on changes in X-ray attenuation is the principle of this method and calibration and image processing procedures are needed for the better image and reproducible measurements. Using this tool, 3D reconstruction is also possible and it enables to visualize the internal structures of dental caries. With the advances in the computer technology, more diverse applications are being studied, such automated caries assessment algorithms.

• Journal of radiological science and technology
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• v.25 no.2
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• pp.57-64
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• 2002

The recent development of embodiment technology of the medical images makes most medical institutions introduce PACS(Picture Archiving and Communication System) in haste. However lots of PACS solutions, currently developed and distributed, haven't been able to serve the convenience of users and to satisfy user's demand because of economic limitations and administrator-oriented considerations in the process of development. So we have developed MIH(Medical Image History), by which we can search and refer to the patient's medical images and information with few restrictions of time and space for diagnosis and treatment. This program will contribute to the improvement in the medical environment and meet the clients' need. We'll make more effort to develop the application which insures the better quality of medical images. MIH manages the patient's image files and medical records like film chart in connection with time. This trial will contribute to the reduction of the economical loss caused by unnecessary references and improve the quality in the medical services. The demand on the development or the program which refers to the medical +ata quickly and keeps them stable will be continued by the medical institute. This will satisfy the client's demand and improve the service to the patients in that tile program will be modified from the standpoint of the users. MIH is trying to keep user-oriented policy and to apply the benefit of the analog system to the digital environment. It is necessary to lead the public to the better understanding that the systematic management and referring of the medical images is as important as the quality of the images.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.27 no.1
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• pp.70-76
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• 2017

Objectives: This on-site study was performed to evaluate the reduction efficiency of an air cleaner on particulate matters and biological agents in a swine facility. Materials and Methods: Particulate matter was measured using a real-time monitoring recorder and biological agents were sampled with a one-stage impactor and then analyzed based on the microbial culture method. An experimental process for the reduction effect on airborne pollutants through air cleaner operation consisted of three conditions: no treatment, wet scrapper by water spray and wet scrapper by disinfectant spray. Results: Geometric mean levels of particulate matter(TSP, $PM_{10}$, $PM_{2.5}$ and $PM_1$) were presented at $1,608{\mu}g/m^3$, $1,373.8{\mu}g/m^3$, $401.8{\mu}g/m^3$ and $144.5{\mu}g/m^3$ for no treatment; $1,503{\mu}g/m^3$, $1,017{\mu}g/m^3$, $159.4{\mu}g/m^3$ and $69.8{\mu}g/m^3$ for wet scrapper by water spray; and $1,222.17{\mu}g/m^3$, $477.17{\mu}g/m^3$, $33.2{\mu}g/m^3$ and $11.1{\mu}g/m^3$ for wet scrapper by disinfectant spray, respectively. In the case of biological agents, the geometric averaged concentrations of total airborne bacteria and fungi were as follows: $45,371cfu/m^3$ and $13,474cfu/m^3$ for no treatment, $43,286cfu/m^3$ and $8,610cfu/m^3$ for wet scrapper by water spray, and $2,440cfu/m^3$ and 1,867 cfu/ for wet scrapper by disinfectant spray, respectively. Regardless of particulate matter and biological agent, the highest concentrations were found for no treatment, while the lowest concentrations were found with wet scrapper by disinfectant spray. Conclusions: Based on the results obtained from this on-site evaluation, there was a significant reduction effect on particulate matter and biological agents through the application of an air cleaner in this study.